Experiment: KB with Nitrofurantoin 125 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0853 and Psyr_0854 overlap by 4 nucleotides Psyr_0854 and Psyr_0855 overlap by 11 nucleotides Psyr_0855 and Psyr_0856 are separated by 144 nucleotides Psyr_0856 and Psyr_0857 overlap by 8 nucleotides
Psyr_0853: Psyr_0853 - conserved hypothetical protein, at 971,484 to 974,303
_0853
Psyr_0854: Psyr_0854 - conserved hypothetical protein, at 974,300 to 975,007
_0854
Psyr_0855: Psyr_0855 - conserved hypothetical protein, at 974,997 to 976,274
_0855
Psyr_0856: Psyr_0856 - conserved hypothetical protein, at 976,419 to 977,225
_0856
Psyr_0857: Psyr_0857 - [SSU ribosomal protein S18P]-alanine acetyltransferase, at 977,218 to 977,670
_0857
Position (kb)
974
975
976
977 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 974.068 kb on + strand at 974.068 kb on + strand at 974.068 kb on + strand at 974.068 kb on + strand at 974.068 kb on + strand at 974.069 kb on - strand at 974.069 kb on - strand at 974.069 kb on - strand at 974.069 kb on - strand at 974.069 kb on - strand at 974.069 kb on - strand at 974.069 kb on - strand at 974.069 kb on - strand at 974.069 kb on - strand at 974.069 kb on - strand at 974.069 kb on - strand at 974.069 kb on - strand at 974.069 kb on - strand at 974.089 kb on + strand at 974.089 kb on + strand at 974.090 kb on - strand at 974.090 kb on - strand at 974.116 kb on + strand at 974.117 kb on - strand at 974.117 kb on - strand at 974.206 kb on + strand at 974.206 kb on + strand at 974.206 kb on + strand at 974.207 kb on - strand at 974.207 kb on - strand at 974.547 kb on + strand, within Psyr_0854 at 974.548 kb on - strand, within Psyr_0854 at 974.551 kb on + strand, within Psyr_0854 at 974.627 kb on + strand, within Psyr_0854 at 974.627 kb on + strand, within Psyr_0854 at 974.628 kb on - strand, within Psyr_0854 at 974.823 kb on + strand, within Psyr_0854 at 974.823 kb on + strand, within Psyr_0854 at 974.823 kb on + strand, within Psyr_0854 at 974.824 kb on - strand, within Psyr_0854 at 974.824 kb on - strand, within Psyr_0854 at 974.826 kb on + strand, within Psyr_0854 at 974.826 kb on + strand, within Psyr_0854 at 974.826 kb on + strand, within Psyr_0854 at 974.827 kb on - strand, within Psyr_0854 at 974.827 kb on - strand, within Psyr_0854 at 974.827 kb on - strand, within Psyr_0854 at 974.878 kb on + strand, within Psyr_0854 at 974.878 kb on + strand, within Psyr_0854 at 974.879 kb on - strand, within Psyr_0854 at 974.914 kb on - strand, within Psyr_0854 at 974.994 kb on - strand at 975.098 kb on - strand at 975.160 kb on + strand, within Psyr_0855 at 975.352 kb on + strand, within Psyr_0855 at 975.352 kb on + strand, within Psyr_0855 at 975.353 kb on - strand, within Psyr_0855 at 975.353 kb on - strand, within Psyr_0855 at 975.358 kb on + strand, within Psyr_0855 at 975.358 kb on + strand, within Psyr_0855 at 975.358 kb on + strand, within Psyr_0855 at 975.358 kb on + strand, within Psyr_0855 at 975.359 kb on - strand at 975.359 kb on - strand, within Psyr_0855 at 975.359 kb on - strand, within Psyr_0855 at 975.359 kb on - strand, within Psyr_0855 at 975.732 kb on + strand, within Psyr_0855 at 975.732 kb on + strand, within Psyr_0855 at 975.732 kb on + strand, within Psyr_0855 at 975.736 kb on + strand, within Psyr_0855 at 975.736 kb on + strand, within Psyr_0855 at 975.736 kb on + strand, within Psyr_0855 at 975.736 kb on + strand, within Psyr_0855 at 975.737 kb on - strand, within Psyr_0855 at 975.737 kb on - strand, within Psyr_0855 at 975.737 kb on - strand, within Psyr_0855 at 975.737 kb on - strand, within Psyr_0855 at 975.737 kb on - strand, within Psyr_0855 at 975.737 kb on - strand, within Psyr_0855 at 975.737 kb on - strand, within Psyr_0855 at 975.737 kb on - strand, within Psyr_0855 at 975.737 kb on - strand, within Psyr_0855 at 975.858 kb on + strand, within Psyr_0855 at 975.859 kb on - strand, within Psyr_0855 at 975.940 kb on + strand, within Psyr_0855 at 975.970 kb on + strand, within Psyr_0855 at 975.970 kb on + strand, within Psyr_0855 at 975.971 kb on - strand, within Psyr_0855 at 976.190 kb on + strand at 976.190 kb on + strand at 976.191 kb on - strand at 976.313 kb on + strand at 976.313 kb on + strand at 976.313 kb on + strand at 976.313 kb on + strand at 976.332 kb on + strand at 976.332 kb on + strand at 976.332 kb on + strand at 976.333 kb on - strand at 976.333 kb on - strand at 976.333 kb on - strand at 976.333 kb on - strand at 976.380 kb on + strand at 976.380 kb on + strand at 976.394 kb on + strand at 976.399 kb on + strand at 977.231 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Nitrofurantoin 125 ug/ml remove 974,068 + +0.4 974,068 + -0.7 974,068 + +0.6 974,068 + -0.1 974,068 + +1.2 974,069 - +0.8 974,069 - -0.3 974,069 - +1.4 974,069 - +0.5 974,069 - -0.8 974,069 - -0.8 974,069 - -0.4 974,069 - +0.0 974,069 - +1.1 974,069 - -0.1 974,069 - -0.1 974,069 - +0.7 974,069 - +1.4 974,089 + +0.3 974,089 + +0.4 974,090 - -0.2 974,090 - -2.0 974,116 + -0.2 974,117 - +0.4 974,117 - +0.2 974,206 + +1.5 974,206 + -0.8 974,206 + +0.8 974,207 - +0.8 974,207 - -1.5 974,547 + Psyr_0854 0.35 +0.2 974,548 - Psyr_0854 0.35 +0.1 974,551 + Psyr_0854 0.35 -0.9 974,627 + Psyr_0854 0.46 -0.8 974,627 + Psyr_0854 0.46 -0.2 974,628 - Psyr_0854 0.46 +0.0 974,823 + Psyr_0854 0.74 -0.8 974,823 + Psyr_0854 0.74 +0.4 974,823 + Psyr_0854 0.74 -0.7 974,824 - Psyr_0854 0.74 -0.2 974,824 - Psyr_0854 0.74 +0.2 974,826 + Psyr_0854 0.74 +0.4 974,826 + Psyr_0854 0.74 +0.7 974,826 + Psyr_0854 0.74 +0.5 974,827 - Psyr_0854 0.74 +0.0 974,827 - Psyr_0854 0.74 +0.2 974,827 - Psyr_0854 0.74 +0.4 974,878 + Psyr_0854 0.82 +0.6 974,878 + Psyr_0854 0.82 +0.2 974,879 - Psyr_0854 0.82 +0.6 974,914 - Psyr_0854 0.87 -0.4 974,994 - +0.4 975,098 - +2.4 975,160 + Psyr_0855 0.13 +1.4 975,352 + Psyr_0855 0.28 +0.2 975,352 + Psyr_0855 0.28 -1.2 975,353 - Psyr_0855 0.28 -0.4 975,353 - Psyr_0855 0.28 +1.0 975,358 + Psyr_0855 0.28 +0.3 975,358 + Psyr_0855 0.28 +0.4 975,358 + Psyr_0855 0.28 +0.8 975,358 + Psyr_0855 0.28 +1.1 975,359 - +0.4 975,359 - Psyr_0855 0.28 +0.4 975,359 - Psyr_0855 0.28 +1.2 975,359 - Psyr_0855 0.28 -0.2 975,732 + Psyr_0855 0.58 +0.5 975,732 + Psyr_0855 0.58 -0.4 975,732 + Psyr_0855 0.58 +1.8 975,736 + Psyr_0855 0.58 -1.8 975,736 + Psyr_0855 0.58 +0.1 975,736 + Psyr_0855 0.58 +0.6 975,736 + Psyr_0855 0.58 +1.7 975,737 - Psyr_0855 0.58 +1.2 975,737 - Psyr_0855 0.58 -0.0 975,737 - Psyr_0855 0.58 +0.6 975,737 - Psyr_0855 0.58 +0.1 975,737 - Psyr_0855 0.58 +0.6 975,737 - Psyr_0855 0.58 +0.5 975,737 - Psyr_0855 0.58 +0.8 975,737 - Psyr_0855 0.58 -0.2 975,737 - Psyr_0855 0.58 +0.8 975,858 + Psyr_0855 0.67 +0.4 975,859 - Psyr_0855 0.67 +0.3 975,940 + Psyr_0855 0.74 +0.0 975,970 + Psyr_0855 0.76 +0.2 975,970 + Psyr_0855 0.76 +0.1 975,971 - Psyr_0855 0.76 -0.2 976,190 + +0.7 976,190 + +0.8 976,191 - -1.2 976,313 + -0.4 976,313 + +0.1 976,313 + +0.1 976,313 + -0.0 976,332 + +0.8 976,332 + +0.6 976,332 + -1.2 976,333 - -0.8 976,333 - +0.2 976,333 - -0.7 976,333 - -1.0 976,380 + +0.4 976,380 + +0.3 976,394 + -1.1 976,399 + +1.2 977,231 + -1.2
Or see this region's nucleotide sequence