Experiment: KB with Nitrofurantoin 125 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0439 and Psyr_0440 are separated by 309 nucleotides Psyr_0440 and Psyr_0441 are separated by 692 nucleotides
Psyr_0439: Psyr_0439 - conserved hypothetical protein, at 476,310 to 477,029
_0439
Psyr_0440: Psyr_0440 - Cobyrinic acid a,c-diamide synthase, at 477,339 to 478,118
_0440
Psyr_0441: Psyr_0441 - conserved hypothetical protein, at 478,811 to 479,206
_0441
Position (kb)
477
478
479 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 476.527 kb on + strand, within Psyr_0439 at 476.527 kb on + strand, within Psyr_0439 at 476.566 kb on - strand, within Psyr_0439 at 476.566 kb on - strand, within Psyr_0439 at 476.566 kb on - strand, within Psyr_0439 at 476.614 kb on + strand, within Psyr_0439 at 476.614 kb on + strand, within Psyr_0439 at 476.614 kb on + strand, within Psyr_0439 at 476.614 kb on + strand, within Psyr_0439 at 476.614 kb on + strand, within Psyr_0439 at 476.615 kb on - strand, within Psyr_0439 at 476.615 kb on - strand, within Psyr_0439 at 477.066 kb on + strand at 477.067 kb on - strand at 477.141 kb on + strand at 477.141 kb on + strand at 477.141 kb on + strand at 477.142 kb on - strand at 477.145 kb on - strand at 477.160 kb on + strand at 477.160 kb on + strand at 477.161 kb on - strand at 477.181 kb on + strand at 477.181 kb on + strand at 477.181 kb on + strand at 477.181 kb on + strand at 477.182 kb on - strand at 477.182 kb on - strand at 477.256 kb on + strand at 477.257 kb on - strand at 477.267 kb on + strand at 477.268 kb on - strand at 477.268 kb on - strand at 477.437 kb on - strand, within Psyr_0440 at 477.474 kb on + strand, within Psyr_0440 at 477.576 kb on - strand, within Psyr_0440 at 477.579 kb on + strand, within Psyr_0440 at 477.579 kb on + strand, within Psyr_0440 at 477.579 kb on + strand, within Psyr_0440 at 477.579 kb on + strand, within Psyr_0440 at 477.580 kb on - strand, within Psyr_0440 at 477.580 kb on - strand, within Psyr_0440 at 477.580 kb on - strand, within Psyr_0440 at 477.580 kb on - strand, within Psyr_0440 at 477.580 kb on - strand, within Psyr_0440 at 477.716 kb on + strand, within Psyr_0440 at 477.717 kb on - strand, within Psyr_0440 at 477.829 kb on - strand, within Psyr_0440 at 477.984 kb on + strand, within Psyr_0440 at 477.984 kb on + strand, within Psyr_0440 at 478.144 kb on + strand at 478.145 kb on - strand at 478.303 kb on - strand at 478.303 kb on - strand at 478.303 kb on - strand at 478.332 kb on - strand at 478.332 kb on - strand at 478.332 kb on - strand at 478.363 kb on + strand at 478.363 kb on + strand at 478.492 kb on + strand at 478.492 kb on + strand at 478.492 kb on + strand at 478.493 kb on - strand at 478.493 kb on - strand at 478.493 kb on - strand at 478.493 kb on - strand at 478.493 kb on - strand at 478.493 kb on - strand at 478.493 kb on - strand at 478.493 kb on - strand at 478.494 kb on + strand at 478.494 kb on + strand at 478.494 kb on + strand at 478.494 kb on + strand at 478.495 kb on - strand at 478.495 kb on - strand at 478.495 kb on - strand at 478.743 kb on + strand at 478.743 kb on + strand at 478.743 kb on + strand at 478.743 kb on + strand at 478.743 kb on + strand at 478.812 kb on + strand at 478.829 kb on + strand at 478.829 kb on + strand at 478.829 kb on + strand at 478.829 kb on + strand at 478.830 kb on - strand at 478.830 kb on - strand at 478.830 kb on - strand at 478.830 kb on - strand at 478.830 kb on - strand at 478.830 kb on - strand at 478.830 kb on - strand at 478.830 kb on - strand at 478.830 kb on - strand at 478.830 kb on - strand at 478.969 kb on + strand, within Psyr_0441 at 478.970 kb on - strand, within Psyr_0441 at 479.041 kb on - strand, within Psyr_0441 at 479.041 kb on - strand, within Psyr_0441 at 479.041 kb on - strand, within Psyr_0441
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Nitrofurantoin 125 ug/ml remove 476,527 + Psyr_0439 0.30 -0.5 476,527 + Psyr_0439 0.30 +0.1 476,566 - Psyr_0439 0.36 +0.2 476,566 - Psyr_0439 0.36 +0.2 476,566 - Psyr_0439 0.36 -0.4 476,614 + Psyr_0439 0.42 +0.3 476,614 + Psyr_0439 0.42 -1.2 476,614 + Psyr_0439 0.42 +0.4 476,614 + Psyr_0439 0.42 +1.0 476,614 + Psyr_0439 0.42 -0.3 476,615 - Psyr_0439 0.42 +0.1 476,615 - Psyr_0439 0.42 +0.5 477,066 + -1.0 477,067 - +0.4 477,141 + -3.3 477,141 + -0.3 477,141 + +0.2 477,142 - -0.1 477,145 - -2.5 477,160 + -0.6 477,160 + -1.6 477,161 - -0.6 477,181 + -0.6 477,181 + -0.1 477,181 + +0.3 477,181 + -1.0 477,182 - +0.3 477,182 - +0.7 477,256 + -1.1 477,257 - +0.0 477,267 + -1.2 477,268 - +1.0 477,268 - +0.5 477,437 - Psyr_0440 0.13 +0.3 477,474 + Psyr_0440 0.17 +0.0 477,576 - Psyr_0440 0.30 -0.5 477,579 + Psyr_0440 0.31 -0.7 477,579 + Psyr_0440 0.31 -0.4 477,579 + Psyr_0440 0.31 -0.4 477,579 + Psyr_0440 0.31 -0.4 477,580 - Psyr_0440 0.31 -0.7 477,580 - Psyr_0440 0.31 +0.0 477,580 - Psyr_0440 0.31 +0.0 477,580 - Psyr_0440 0.31 -0.9 477,580 - Psyr_0440 0.31 -0.2 477,716 + Psyr_0440 0.48 -1.0 477,717 - Psyr_0440 0.48 -0.1 477,829 - Psyr_0440 0.63 -0.8 477,984 + Psyr_0440 0.83 +0.5 477,984 + Psyr_0440 0.83 +0.2 478,144 + -0.4 478,145 - -0.6 478,303 - +0.8 478,303 - +0.2 478,303 - +1.8 478,332 - -0.2 478,332 - -0.4 478,332 - -0.2 478,363 + +1.0 478,363 + +0.6 478,492 + -1.2 478,492 + +1.0 478,492 + -1.1 478,493 - -0.6 478,493 - +0.0 478,493 - -0.4 478,493 - -0.4 478,493 - +0.3 478,493 - -2.5 478,493 - +0.0 478,493 - +0.1 478,494 + -2.0 478,494 + +0.3 478,494 + -0.2 478,494 + +0.0 478,495 - +0.8 478,495 - +0.4 478,495 - -0.6 478,743 + +2.6 478,743 + -0.3 478,743 + -1.3 478,743 + -0.9 478,743 + -0.1 478,812 + +0.1 478,829 + -0.7 478,829 + -1.1 478,829 + +0.7 478,829 + +0.1 478,830 - +1.1 478,830 - -0.3 478,830 - +0.4 478,830 - -1.2 478,830 - +0.1 478,830 - -0.4 478,830 - -0.5 478,830 - -0.6 478,830 - +0.5 478,830 - -0.4 478,969 + Psyr_0441 0.40 +0.9 478,970 - Psyr_0441 0.40 +0.1 479,041 - Psyr_0441 0.58 +3.0 479,041 - Psyr_0441 0.58 +1.4 479,041 - Psyr_0441 0.58 -0.3
Or see this region's nucleotide sequence