Experiment: KB with Nitrofurantoin 125 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0260 and Psyr_0261 are separated by 224 nucleotides Psyr_0261 and Psyr_0262 are separated by 228 nucleotides Psyr_0262 and Psyr_0263 are separated by 197 nucleotides
Psyr_0260: Psyr_0260 - conserved hypothetical protein, at 276,603 to 277,070
_0260
Psyr_0261: Psyr_0261 - conserved hypothetical protein, at 277,295 to 277,582
_0261
Psyr_0262: Psyr_0262 - Transport-associated protein, at 277,811 to 278,650
_0262
Psyr_0263: Psyr_0263 - Two-component response regulator AlgB, at 278,848 to 280,194
_0263
Position (kb) 277
278
279 Strain fitness (log2 ratio) -2
-1
0
1
2 at 276.835 kb on + strand, within Psyr_0260 at 276.835 kb on + strand, within Psyr_0260 at 276.835 kb on + strand, within Psyr_0260 at 276.835 kb on + strand, within Psyr_0260 at 276.835 kb on + strand, within Psyr_0260 at 276.835 kb on + strand, within Psyr_0260 at 276.835 kb on + strand, within Psyr_0260 at 276.835 kb on + strand, within Psyr_0260 at 276.835 kb on + strand, within Psyr_0260 at 276.836 kb on - strand, within Psyr_0260 at 276.836 kb on - strand, within Psyr_0260 at 276.836 kb on - strand, within Psyr_0260 at 276.836 kb on - strand, within Psyr_0260 at 276.836 kb on - strand, within Psyr_0260 at 276.836 kb on - strand, within Psyr_0260 at 276.836 kb on - strand, within Psyr_0260 at 276.836 kb on - strand, within Psyr_0260 at 276.836 kb on - strand, within Psyr_0260 at 277.071 kb on + strand at 277.187 kb on + strand at 277.187 kb on + strand at 277.187 kb on + strand at 277.243 kb on + strand at 277.244 kb on - strand at 277.263 kb on + strand at 277.263 kb on + strand at 277.263 kb on + strand at 277.267 kb on - strand at 277.331 kb on + strand, within Psyr_0261 at 277.331 kb on + strand, within Psyr_0261 at 277.332 kb on - strand, within Psyr_0261 at 277.332 kb on - strand, within Psyr_0261 at 277.332 kb on - strand, within Psyr_0261 at 277.393 kb on + strand, within Psyr_0261 at 277.404 kb on + strand, within Psyr_0261 at 277.552 kb on + strand, within Psyr_0261 at 277.552 kb on + strand, within Psyr_0261 at 277.553 kb on - strand, within Psyr_0261 at 277.567 kb on + strand at 277.567 kb on + strand at 277.567 kb on + strand at 277.568 kb on - strand at 277.568 kb on - strand at 277.736 kb on - strand at 277.874 kb on - strand at 277.992 kb on + strand, within Psyr_0262 at 277.992 kb on + strand, within Psyr_0262 at 277.992 kb on + strand, within Psyr_0262 at 277.992 kb on + strand, within Psyr_0262 at 277.992 kb on + strand, within Psyr_0262 at 277.992 kb on + strand, within Psyr_0262 at 277.992 kb on + strand, within Psyr_0262 at 277.993 kb on - strand, within Psyr_0262 at 277.993 kb on - strand, within Psyr_0262 at 277.993 kb on - strand, within Psyr_0262 at 277.993 kb on - strand, within Psyr_0262 at 277.993 kb on - strand, within Psyr_0262 at 278.650 kb on + strand at 278.651 kb on - strand at 278.706 kb on + strand at 278.706 kb on + strand at 278.736 kb on + strand at 278.737 kb on - strand at 278.753 kb on - strand at 278.753 kb on - strand at 278.814 kb on + strand at 278.814 kb on + strand at 278.840 kb on + strand at 278.944 kb on + strand at 278.945 kb on - strand at 278.945 kb on - strand at 278.945 kb on - strand at 279.002 kb on + strand, within Psyr_0263 at 279.003 kb on - strand, within Psyr_0263 at 279.163 kb on + strand, within Psyr_0263 at 279.164 kb on - strand, within Psyr_0263 at 279.650 kb on - strand, within Psyr_0263
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Nitrofurantoin 125 ug/ml remove 276,835 + Psyr_0260 0.50 -2.3 276,835 + Psyr_0260 0.50 +0.5 276,835 + Psyr_0260 0.50 +0.5 276,835 + Psyr_0260 0.50 -0.3 276,835 + Psyr_0260 0.50 +0.5 276,835 + Psyr_0260 0.50 +0.1 276,835 + Psyr_0260 0.50 -0.1 276,835 + Psyr_0260 0.50 +0.8 276,835 + Psyr_0260 0.50 -0.2 276,836 - Psyr_0260 0.50 +1.8 276,836 - Psyr_0260 0.50 +1.5 276,836 - Psyr_0260 0.50 +0.1 276,836 - Psyr_0260 0.50 -0.6 276,836 - Psyr_0260 0.50 -0.2 276,836 - Psyr_0260 0.50 +0.5 276,836 - Psyr_0260 0.50 -2.4 276,836 - Psyr_0260 0.50 +0.1 276,836 - Psyr_0260 0.50 -0.6 277,071 + +0.5 277,187 + +0.2 277,187 + -0.9 277,187 + +1.1 277,243 + +0.5 277,244 - -0.2 277,263 + -0.0 277,263 + -1.8 277,263 + +0.9 277,267 - +0.8 277,331 + Psyr_0261 0.12 -0.1 277,331 + Psyr_0261 0.12 -0.1 277,332 - Psyr_0261 0.13 -0.9 277,332 - Psyr_0261 0.13 +0.2 277,332 - Psyr_0261 0.13 +0.3 277,393 + Psyr_0261 0.34 +0.6 277,404 + Psyr_0261 0.38 +0.6 277,552 + Psyr_0261 0.89 -0.4 277,552 + Psyr_0261 0.89 +0.1 277,553 - Psyr_0261 0.90 -0.1 277,567 + +0.1 277,567 + +0.1 277,567 + +1.6 277,568 - +0.1 277,568 - -0.1 277,736 - +0.2 277,874 - +0.1 277,992 + Psyr_0262 0.22 +0.4 277,992 + Psyr_0262 0.22 +1.4 277,992 + Psyr_0262 0.22 +0.2 277,992 + Psyr_0262 0.22 +1.1 277,992 + Psyr_0262 0.22 -0.9 277,992 + Psyr_0262 0.22 +0.5 277,992 + Psyr_0262 0.22 +0.5 277,993 - Psyr_0262 0.22 -0.1 277,993 - Psyr_0262 0.22 +0.1 277,993 - Psyr_0262 0.22 -0.4 277,993 - Psyr_0262 0.22 +0.2 277,993 - Psyr_0262 0.22 +1.2 278,650 + +0.1 278,651 - +0.7 278,706 + -0.7 278,706 + -0.4 278,736 + -0.4 278,737 - +0.2 278,753 - +1.1 278,753 - +0.5 278,814 + -0.5 278,814 + +1.7 278,840 + +0.8 278,944 + +1.4 278,945 - +1.4 278,945 - +2.0 278,945 - +1.6 279,002 + Psyr_0263 0.11 +2.4 279,003 - Psyr_0263 0.12 -0.4 279,163 + Psyr_0263 0.23 +2.6 279,164 - Psyr_0263 0.23 +0.4 279,650 - Psyr_0263 0.60 +0.8
Or see this region's nucleotide sequence