Experiment: KB with Nitrofurantoin 125 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0131 and Psyr_0132 are separated by 100 nucleotides Psyr_0132 and Psyr_0133 are separated by 83 nucleotides
Psyr_0131: Psyr_0131 - HAD-superfamily hydrolase, subfamily IA, variant 3:HAD-superfamily hydrolase, subfamily IA, variant 1, at 139,587 to 140,261
_0131
Psyr_0132: Psyr_0132 - conserved hypothetical protein, at 140,362 to 140,610
_0132
Psyr_0133: Psyr_0133 - transcriptional regulator, AsnC family, at 140,694 to 141,164
_0133
Position (kb)
140
141 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 139.460 kb on + strand at 139.580 kb on + strand at 139.586 kb on + strand at 139.586 kb on + strand at 139.587 kb on - strand at 139.710 kb on - strand, within Psyr_0131 at 139.710 kb on - strand, within Psyr_0131 at 139.827 kb on + strand at 139.828 kb on - strand, within Psyr_0131 at 140.014 kb on - strand, within Psyr_0131 at 140.014 kb on - strand, within Psyr_0131 at 140.059 kb on + strand, within Psyr_0131 at 140.059 kb on + strand, within Psyr_0131 at 140.059 kb on + strand, within Psyr_0131 at 140.059 kb on + strand, within Psyr_0131 at 140.059 kb on + strand, within Psyr_0131 at 140.059 kb on + strand, within Psyr_0131 at 140.060 kb on - strand, within Psyr_0131 at 140.205 kb on + strand at 140.205 kb on + strand at 140.205 kb on + strand at 140.205 kb on + strand at 140.206 kb on - strand at 140.206 kb on - strand at 140.206 kb on - strand at 140.206 kb on - strand at 140.206 kb on - strand at 140.206 kb on - strand at 140.206 kb on - strand at 140.206 kb on - strand at 140.206 kb on - strand at 140.206 kb on - strand at 140.265 kb on + strand at 140.305 kb on + strand at 140.305 kb on + strand at 140.306 kb on - strand at 140.306 kb on - strand at 140.306 kb on - strand at 140.306 kb on - strand at 140.306 kb on - strand at 140.306 kb on - strand at 140.355 kb on + strand at 140.355 kb on + strand at 140.355 kb on + strand at 140.355 kb on + strand at 140.355 kb on + strand at 140.356 kb on - strand at 140.356 kb on - strand at 140.356 kb on - strand at 140.435 kb on + strand, within Psyr_0132 at 140.435 kb on + strand, within Psyr_0132 at 140.436 kb on - strand, within Psyr_0132 at 140.436 kb on - strand, within Psyr_0132 at 140.436 kb on - strand, within Psyr_0132 at 140.436 kb on - strand, within Psyr_0132 at 140.436 kb on - strand, within Psyr_0132 at 140.540 kb on + strand, within Psyr_0132 at 140.593 kb on + strand at 140.593 kb on + strand at 140.593 kb on + strand at 140.593 kb on + strand at 140.593 kb on + strand at 140.593 kb on + strand at 140.593 kb on + strand at 140.593 kb on + strand at 140.593 kb on + strand at 140.593 kb on + strand at 140.593 kb on + strand at 140.594 kb on - strand at 140.594 kb on - strand at 140.594 kb on - strand at 140.594 kb on - strand at 140.594 kb on - strand at 140.713 kb on + strand at 140.713 kb on + strand at 140.714 kb on - strand at 140.714 kb on - strand at 140.806 kb on + strand, within Psyr_0133 at 140.807 kb on - strand, within Psyr_0133 at 140.807 kb on - strand, within Psyr_0133 at 140.854 kb on + strand, within Psyr_0133 at 140.854 kb on + strand, within Psyr_0133 at 140.854 kb on + strand, within Psyr_0133 at 140.854 kb on + strand, within Psyr_0133 at 140.855 kb on - strand, within Psyr_0133 at 140.855 kb on - strand, within Psyr_0133 at 140.855 kb on - strand, within Psyr_0133 at 140.855 kb on - strand, within Psyr_0133 at 140.855 kb on - strand, within Psyr_0133 at 140.855 kb on - strand, within Psyr_0133 at 140.855 kb on - strand, within Psyr_0133 at 140.907 kb on + strand, within Psyr_0133 at 140.908 kb on - strand, within Psyr_0133 at 141.071 kb on + strand, within Psyr_0133 at 141.190 kb on + strand at 141.190 kb on + strand at 141.191 kb on - strand at 141.191 kb on - strand at 141.191 kb on - strand at 141.191 kb on - strand at 141.191 kb on - strand at 141.191 kb on - strand at 141.191 kb on - strand at 141.191 kb on - strand at 141.233 kb on - strand at 141.233 kb on - strand at 141.233 kb on - strand at 141.233 kb on - strand at 141.233 kb on - strand at 141.561 kb on - strand at 141.561 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Nitrofurantoin 125 ug/ml remove 139,460 + +0.9 139,580 + -0.2 139,586 + +0.1 139,586 + +1.2 139,587 - -0.5 139,710 - Psyr_0131 0.18 -0.9 139,710 - Psyr_0131 0.18 -0.1 139,827 + +0.5 139,828 - Psyr_0131 0.36 -0.1 140,014 - Psyr_0131 0.63 +0.7 140,014 - Psyr_0131 0.63 -0.5 140,059 + Psyr_0131 0.70 -0.9 140,059 + Psyr_0131 0.70 +0.4 140,059 + Psyr_0131 0.70 +0.7 140,059 + Psyr_0131 0.70 +0.5 140,059 + Psyr_0131 0.70 +0.8 140,059 + Psyr_0131 0.70 -0.2 140,060 - Psyr_0131 0.70 -0.1 140,205 + -1.0 140,205 + -0.6 140,205 + -0.1 140,205 + -0.3 140,206 - -0.8 140,206 - +0.5 140,206 - -1.2 140,206 - -0.4 140,206 - -1.9 140,206 - -2.1 140,206 - -0.3 140,206 - -1.4 140,206 - -0.4 140,206 - +0.7 140,265 + +0.6 140,305 + +0.1 140,305 + -0.4 140,306 - +0.7 140,306 - +0.4 140,306 - -1.1 140,306 - +1.3 140,306 - -0.1 140,306 - +0.0 140,355 + +0.6 140,355 + +1.1 140,355 + -0.1 140,355 + +0.6 140,355 + -0.9 140,356 - -0.4 140,356 - +0.9 140,356 - -0.1 140,435 + Psyr_0132 0.29 -0.9 140,435 + Psyr_0132 0.29 -0.1 140,436 - Psyr_0132 0.30 -0.3 140,436 - Psyr_0132 0.30 +1.7 140,436 - Psyr_0132 0.30 -0.5 140,436 - Psyr_0132 0.30 -2.1 140,436 - Psyr_0132 0.30 -1.6 140,540 + Psyr_0132 0.71 -1.4 140,593 + +0.5 140,593 + +0.3 140,593 + -0.2 140,593 + -0.1 140,593 + -0.4 140,593 + -0.4 140,593 + +0.1 140,593 + -0.0 140,593 + +0.1 140,593 + -0.9 140,593 + -0.2 140,594 - -0.8 140,594 - -0.1 140,594 - +1.1 140,594 - +0.3 140,594 - -0.9 140,713 + +0.1 140,713 + +0.1 140,714 - -0.6 140,714 - +0.1 140,806 + Psyr_0133 0.24 -0.1 140,807 - Psyr_0133 0.24 +1.4 140,807 - Psyr_0133 0.24 -0.3 140,854 + Psyr_0133 0.34 +0.0 140,854 + Psyr_0133 0.34 -0.3 140,854 + Psyr_0133 0.34 +0.5 140,854 + Psyr_0133 0.34 -0.1 140,855 - Psyr_0133 0.34 -0.1 140,855 - Psyr_0133 0.34 -0.5 140,855 - Psyr_0133 0.34 +0.3 140,855 - Psyr_0133 0.34 -2.2 140,855 - Psyr_0133 0.34 +0.9 140,855 - Psyr_0133 0.34 +1.1 140,855 - Psyr_0133 0.34 -0.2 140,907 + Psyr_0133 0.45 +0.1 140,908 - Psyr_0133 0.45 -0.2 141,071 + Psyr_0133 0.80 -0.3 141,190 + +0.9 141,190 + +1.2 141,191 - +0.1 141,191 - +1.7 141,191 - -0.5 141,191 - +0.8 141,191 - +1.7 141,191 - +0.2 141,191 - -0.5 141,191 - +0.6 141,233 - +0.4 141,233 - -0.2 141,233 - +1.9 141,233 - +0.4 141,233 - +0.1 141,561 - -0.4 141,561 - -0.3
Or see this region's nucleotide sequence