Experiment: D-Mannose (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5621 and Pf6N2E2_5622 are separated by 7 nucleotides Pf6N2E2_5622 and Pf6N2E2_5623 are separated by 107 nucleotides Pf6N2E2_5623 and Pf6N2E2_5624 are separated by 1 nucleotides
Pf6N2E2_5621 - DNA-binding response regulator, at 6,438,595 to 6,439,272
_5621
Pf6N2E2_5622 - Translation initiation factor 2 (IF-2; GTPase), at 6,439,280 to 6,439,690
_5622
Pf6N2E2_5623 - YciL protein, at 6,439,798 to 6,440,097
_5623
Pf6N2E2_5624 - Intracellular septation protein IspA, at 6,440,099 to 6,440,695
_5624
Position (kb)
6439
6440 Strain fitness (log2 ratio)
-1
0
1 at 6438.367 kb on + strand at 6438.551 kb on + strand at 6438.551 kb on + strand at 6438.551 kb on + strand at 6438.552 kb on - strand at 6438.554 kb on + strand at 6438.554 kb on + strand at 6438.554 kb on + strand at 6438.555 kb on - strand at 6438.555 kb on - strand at 6438.555 kb on - strand at 6438.561 kb on + strand at 6438.561 kb on + strand at 6438.561 kb on + strand at 6438.561 kb on + strand at 6438.561 kb on + strand at 6438.561 kb on + strand at 6438.562 kb on - strand at 6438.571 kb on - strand at 6438.571 kb on - strand at 6438.578 kb on - strand at 6438.578 kb on - strand at 6438.578 kb on - strand at 6438.578 kb on - strand at 6438.580 kb on + strand at 6438.580 kb on + strand at 6438.580 kb on + strand at 6438.581 kb on - strand at 6438.605 kb on + strand at 6438.605 kb on + strand at 6438.605 kb on + strand at 6438.605 kb on + strand at 6438.605 kb on + strand at 6438.606 kb on - strand at 6438.608 kb on + strand at 6438.608 kb on + strand at 6438.608 kb on + strand at 6438.608 kb on + strand at 6438.608 kb on + strand at 6438.609 kb on - strand at 6438.611 kb on + strand at 6438.694 kb on - strand, within Pf6N2E2_5621 at 6438.710 kb on + strand, within Pf6N2E2_5621 at 6438.710 kb on + strand, within Pf6N2E2_5621 at 6438.923 kb on - strand, within Pf6N2E2_5621 at 6438.977 kb on + strand, within Pf6N2E2_5621 at 6438.978 kb on - strand, within Pf6N2E2_5621 at 6439.124 kb on - strand, within Pf6N2E2_5621 at 6439.253 kb on - strand at 6439.253 kb on - strand at 6439.259 kb on - strand at 6439.259 kb on - strand at 6439.306 kb on + strand at 6439.306 kb on + strand at 6439.306 kb on + strand at 6439.306 kb on + strand at 6439.306 kb on + strand at 6439.327 kb on + strand, within Pf6N2E2_5622 at 6439.327 kb on + strand, within Pf6N2E2_5622 at 6439.347 kb on - strand, within Pf6N2E2_5622 at 6439.665 kb on - strand at 6439.665 kb on - strand at 6439.717 kb on + strand at 6439.718 kb on - strand at 6439.718 kb on - strand at 6439.718 kb on - strand at 6439.800 kb on - strand at 6439.841 kb on + strand, within Pf6N2E2_5623 at 6439.841 kb on + strand, within Pf6N2E2_5623 at 6439.842 kb on - strand, within Pf6N2E2_5623 at 6439.987 kb on + strand, within Pf6N2E2_5623 at 6440.090 kb on + strand at 6440.090 kb on + strand at 6440.120 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Mannose (C) remove 6,438,367 + +1.0 6,438,551 + -0.6 6,438,551 + +0.3 6,438,551 + -0.7 6,438,552 - +1.4 6,438,554 + -0.8 6,438,554 + -0.7 6,438,554 + +0.4 6,438,555 - +0.2 6,438,555 - +0.0 6,438,555 - +0.4 6,438,561 + -1.0 6,438,561 + +0.5 6,438,561 + +0.2 6,438,561 + -0.3 6,438,561 + -0.0 6,438,561 + -0.4 6,438,562 - -1.3 6,438,571 - -0.0 6,438,571 - +1.4 6,438,578 - -0.5 6,438,578 - -1.2 6,438,578 - -0.1 6,438,578 - +0.3 6,438,580 + +0.7 6,438,580 + +0.3 6,438,580 + +0.1 6,438,581 - +0.5 6,438,605 + +0.8 6,438,605 + -1.0 6,438,605 + +0.3 6,438,605 + -0.8 6,438,605 + -0.5 6,438,606 - -0.2 6,438,608 + -0.0 6,438,608 + -0.4 6,438,608 + +0.2 6,438,608 + +0.3 6,438,608 + +1.4 6,438,609 - +0.2 6,438,611 + +0.7 6,438,694 - Pf6N2E2_5621 0.15 -0.4 6,438,710 + Pf6N2E2_5621 0.17 +1.4 6,438,710 + Pf6N2E2_5621 0.17 -0.7 6,438,923 - Pf6N2E2_5621 0.48 +0.7 6,438,977 + Pf6N2E2_5621 0.56 +0.6 6,438,978 - Pf6N2E2_5621 0.56 +1.5 6,439,124 - Pf6N2E2_5621 0.78 -0.9 6,439,253 - +0.7 6,439,253 - -0.8 6,439,259 - +0.9 6,439,259 - +1.3 6,439,306 + +0.3 6,439,306 + +0.9 6,439,306 + -1.5 6,439,306 + +0.9 6,439,306 + -0.6 6,439,327 + Pf6N2E2_5622 0.11 -0.4 6,439,327 + Pf6N2E2_5622 0.11 -0.2 6,439,347 - Pf6N2E2_5622 0.16 -1.0 6,439,665 - +0.2 6,439,665 - -0.1 6,439,717 + -0.1 6,439,718 - +1.1 6,439,718 - +0.4 6,439,718 - +0.3 6,439,800 - +0.7 6,439,841 + Pf6N2E2_5623 0.14 +0.5 6,439,841 + Pf6N2E2_5623 0.14 -0.9 6,439,842 - Pf6N2E2_5623 0.15 +0.7 6,439,987 + Pf6N2E2_5623 0.63 +0.2 6,440,090 + -1.4 6,440,090 + +0.5 6,440,120 - -0.5
Or see this region's nucleotide sequence