Experiment: D-Mannose (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1521 and Pf6N2E2_1522 are separated by 170 nucleotides Pf6N2E2_1522 and Pf6N2E2_1523 overlap by 11 nucleotides
Pf6N2E2_1521 - Xylulose-5-phosphate phosphoketolase (EC 4.1.2.9) @ Fructose-6-phosphate phosphoketolase (EC 4.1.2.22), at 1,777,559 to 1,779,931
_1521
Pf6N2E2_1522 - Alcohol dehydrogenase (EC 1.1.1.1), at 1,780,102 to 1,781,085
_1522
Pf6N2E2_1523 - Universal stress protein family 5, at 1,781,075 to 1,781,968
_1523
Position (kb)
1780
1781
1782 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1779.115 kb on + strand, within Pf6N2E2_1521 at 1779.116 kb on - strand, within Pf6N2E2_1521 at 1779.234 kb on + strand, within Pf6N2E2_1521 at 1779.234 kb on + strand, within Pf6N2E2_1521 at 1779.255 kb on + strand, within Pf6N2E2_1521 at 1779.255 kb on + strand, within Pf6N2E2_1521 at 1779.255 kb on + strand, within Pf6N2E2_1521 at 1779.255 kb on + strand, within Pf6N2E2_1521 at 1779.255 kb on + strand, within Pf6N2E2_1521 at 1779.256 kb on - strand, within Pf6N2E2_1521 at 1779.256 kb on - strand, within Pf6N2E2_1521 at 1779.256 kb on - strand, within Pf6N2E2_1521 at 1779.256 kb on - strand, within Pf6N2E2_1521 at 1779.256 kb on - strand, within Pf6N2E2_1521 at 1779.376 kb on + strand, within Pf6N2E2_1521 at 1779.474 kb on + strand, within Pf6N2E2_1521 at 1779.551 kb on + strand, within Pf6N2E2_1521 at 1779.621 kb on + strand, within Pf6N2E2_1521 at 1779.637 kb on + strand, within Pf6N2E2_1521 at 1779.648 kb on + strand, within Pf6N2E2_1521 at 1779.649 kb on - strand, within Pf6N2E2_1521 at 1779.649 kb on - strand, within Pf6N2E2_1521 at 1779.649 kb on - strand, within Pf6N2E2_1521 at 1779.846 kb on + strand at 1779.846 kb on + strand at 1779.846 kb on + strand at 1779.847 kb on - strand at 1779.895 kb on + strand at 1779.896 kb on - strand at 1780.001 kb on + strand at 1780.002 kb on - strand at 1780.022 kb on - strand at 1780.029 kb on + strand at 1780.029 kb on + strand at 1780.030 kb on - strand at 1780.030 kb on - strand at 1780.465 kb on + strand, within Pf6N2E2_1522 at 1780.604 kb on - strand, within Pf6N2E2_1522 at 1780.666 kb on + strand, within Pf6N2E2_1522 at 1780.666 kb on + strand, within Pf6N2E2_1522 at 1780.666 kb on + strand, within Pf6N2E2_1522 at 1780.666 kb on + strand, within Pf6N2E2_1522 at 1780.667 kb on - strand, within Pf6N2E2_1522 at 1781.006 kb on - strand at 1781.072 kb on + strand at 1781.073 kb on - strand at 1781.073 kb on - strand at 1781.112 kb on + strand at 1781.112 kb on + strand at 1781.113 kb on - strand at 1781.290 kb on + strand, within Pf6N2E2_1523 at 1781.291 kb on - strand, within Pf6N2E2_1523 at 1781.397 kb on + strand, within Pf6N2E2_1523 at 1781.398 kb on - strand, within Pf6N2E2_1523 at 1781.626 kb on + strand, within Pf6N2E2_1523 at 1781.703 kb on - strand, within Pf6N2E2_1523 at 1781.822 kb on + strand, within Pf6N2E2_1523 at 1781.822 kb on + strand, within Pf6N2E2_1523 at 1781.822 kb on + strand, within Pf6N2E2_1523 at 1781.823 kb on - strand, within Pf6N2E2_1523 at 1781.823 kb on - strand, within Pf6N2E2_1523 at 1781.946 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Mannose (C) remove 1,779,115 + Pf6N2E2_1521 0.66 +0.1 1,779,116 - Pf6N2E2_1521 0.66 -0.8 1,779,234 + Pf6N2E2_1521 0.71 -0.0 1,779,234 + Pf6N2E2_1521 0.71 -0.6 1,779,255 + Pf6N2E2_1521 0.71 +1.1 1,779,255 + Pf6N2E2_1521 0.71 +2.2 1,779,255 + Pf6N2E2_1521 0.71 -0.7 1,779,255 + Pf6N2E2_1521 0.71 +0.2 1,779,255 + Pf6N2E2_1521 0.71 +0.7 1,779,256 - Pf6N2E2_1521 0.72 -0.8 1,779,256 - Pf6N2E2_1521 0.72 +0.8 1,779,256 - Pf6N2E2_1521 0.72 +0.3 1,779,256 - Pf6N2E2_1521 0.72 +0.4 1,779,256 - Pf6N2E2_1521 0.72 +0.3 1,779,376 + Pf6N2E2_1521 0.77 +0.9 1,779,474 + Pf6N2E2_1521 0.81 -0.9 1,779,551 + Pf6N2E2_1521 0.84 -0.0 1,779,621 + Pf6N2E2_1521 0.87 +0.9 1,779,637 + Pf6N2E2_1521 0.88 +0.1 1,779,648 + Pf6N2E2_1521 0.88 -0.5 1,779,649 - Pf6N2E2_1521 0.88 +0.3 1,779,649 - Pf6N2E2_1521 0.88 -0.5 1,779,649 - Pf6N2E2_1521 0.88 +0.4 1,779,846 + -0.4 1,779,846 + +0.0 1,779,846 + +0.6 1,779,847 - +0.7 1,779,895 + +0.5 1,779,896 - -0.3 1,780,001 + -0.4 1,780,002 - -0.3 1,780,022 - -0.9 1,780,029 + -0.2 1,780,029 + +0.4 1,780,030 - -0.3 1,780,030 - +0.9 1,780,465 + Pf6N2E2_1522 0.37 -0.1 1,780,604 - Pf6N2E2_1522 0.51 -1.6 1,780,666 + Pf6N2E2_1522 0.57 -0.7 1,780,666 + Pf6N2E2_1522 0.57 +0.0 1,780,666 + Pf6N2E2_1522 0.57 -2.7 1,780,666 + Pf6N2E2_1522 0.57 +0.1 1,780,667 - Pf6N2E2_1522 0.57 -2.2 1,781,006 - +1.1 1,781,072 + -0.3 1,781,073 - -0.9 1,781,073 - -0.6 1,781,112 + +0.1 1,781,112 + +0.1 1,781,113 - -1.0 1,781,290 + Pf6N2E2_1523 0.24 -0.5 1,781,291 - Pf6N2E2_1523 0.24 +0.4 1,781,397 + Pf6N2E2_1523 0.36 -0.2 1,781,398 - Pf6N2E2_1523 0.36 +0.1 1,781,626 + Pf6N2E2_1523 0.62 -0.7 1,781,703 - Pf6N2E2_1523 0.70 +0.4 1,781,822 + Pf6N2E2_1523 0.84 +0.4 1,781,822 + Pf6N2E2_1523 0.84 -1.6 1,781,822 + Pf6N2E2_1523 0.84 +0.6 1,781,823 - Pf6N2E2_1523 0.84 -0.1 1,781,823 - Pf6N2E2_1523 0.84 -0.7 1,781,946 - +0.0
Or see this region's nucleotide sequence