Experiment: L-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5603 and Pf1N1B4_5604 are separated by 260 nucleotides Pf1N1B4_5604 and Pf1N1B4_5605 are separated by 84 nucleotides
Pf1N1B4_5603 - Threonine dehydrogenase and related Zn-dependent dehydrogenases, at 26,732 to 27,931
_5603
Pf1N1B4_5604 - Permeases of the major facilitator superfamily, at 28,192 to 29,352
_5604
Pf1N1B4_5605 - LysR family regulatory protein, at 29,437 to 30,378
_5605
Position (kb)
28
29
30 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 27.302 kb on + strand, within Pf1N1B4_5603 at 27.302 kb on + strand at 27.302 kb on + strand, within Pf1N1B4_5603 at 27.303 kb on - strand, within Pf1N1B4_5603 at 27.303 kb on - strand, within Pf1N1B4_5603 at 27.303 kb on - strand, within Pf1N1B4_5603 at 27.303 kb on - strand, within Pf1N1B4_5603 at 27.638 kb on + strand, within Pf1N1B4_5603 at 27.638 kb on + strand, within Pf1N1B4_5603 at 27.639 kb on - strand, within Pf1N1B4_5603 at 27.639 kb on - strand at 27.761 kb on + strand, within Pf1N1B4_5603 at 27.761 kb on + strand, within Pf1N1B4_5603 at 27.762 kb on - strand, within Pf1N1B4_5603 at 27.919 kb on + strand at 28.019 kb on - strand at 28.019 kb on - strand at 28.019 kb on - strand at 28.019 kb on - strand at 28.019 kb on - strand at 28.019 kb on - strand at 28.019 kb on - strand at 28.019 kb on - strand at 28.165 kb on - strand at 28.365 kb on - strand, within Pf1N1B4_5604 at 28.437 kb on - strand, within Pf1N1B4_5604 at 28.530 kb on + strand, within Pf1N1B4_5604 at 28.559 kb on - strand, within Pf1N1B4_5604 at 28.716 kb on - strand, within Pf1N1B4_5604 at 28.716 kb on - strand at 28.975 kb on + strand, within Pf1N1B4_5604 at 28.976 kb on - strand, within Pf1N1B4_5604 at 29.205 kb on - strand, within Pf1N1B4_5604 at 29.324 kb on - strand at 29.324 kb on - strand at 29.324 kb on - strand at 29.406 kb on - strand at 29.625 kb on - strand, within Pf1N1B4_5605 at 29.902 kb on - strand, within Pf1N1B4_5605
Per-strain Table
Position Strand Gene LocusTag Fraction L-Lactate (C) remove 27,302 + Pf1N1B4_5603 0.47 +0.5 27,302 + -1.1 27,302 + Pf1N1B4_5603 0.47 +1.9 27,303 - Pf1N1B4_5603 0.48 -1.4 27,303 - Pf1N1B4_5603 0.48 +0.1 27,303 - Pf1N1B4_5603 0.48 +0.2 27,303 - Pf1N1B4_5603 0.48 -0.6 27,638 + Pf1N1B4_5603 0.76 +0.6 27,638 + Pf1N1B4_5603 0.76 -0.3 27,639 - Pf1N1B4_5603 0.76 -1.0 27,639 - -1.1 27,761 + Pf1N1B4_5603 0.86 -0.7 27,761 + Pf1N1B4_5603 0.86 +0.0 27,762 - Pf1N1B4_5603 0.86 -1.4 27,919 + -0.2 28,019 - +0.1 28,019 - +0.3 28,019 - +0.9 28,019 - -1.1 28,019 - +0.9 28,019 - -0.5 28,019 - -1.7 28,019 - +0.5 28,165 - +0.4 28,365 - Pf1N1B4_5604 0.15 +0.5 28,437 - Pf1N1B4_5604 0.21 +0.2 28,530 + Pf1N1B4_5604 0.29 -0.7 28,559 - Pf1N1B4_5604 0.32 +0.6 28,716 - Pf1N1B4_5604 0.45 +0.5 28,716 - -1.9 28,975 + Pf1N1B4_5604 0.67 +0.6 28,976 - Pf1N1B4_5604 0.68 -1.1 29,205 - Pf1N1B4_5604 0.87 -0.7 29,324 - +0.6 29,324 - -1.7 29,324 - +0.9 29,406 - +0.3 29,625 - Pf1N1B4_5605 0.20 +0.2 29,902 - Pf1N1B4_5605 0.49 +1.7
Or see this region's nucleotide sequence