Experiment: L-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_149 and Pf1N1B4_150 are separated by 304 nucleotides Pf1N1B4_150 and Pf1N1B4_151 are separated by 42 nucleotides
Pf1N1B4_149 - Pyoverdine sidechain non-ribosomal peptide synthetase PvdD, at 188,375 to 191,797
_149
Pf1N1B4_150 - SyrP-like protein, at 192,102 to 193,085
_150
Pf1N1B4_151 - Thioesterase in siderophore biosynthesis gene cluster, at 193,128 to 193,868
_151
Position (kb) 192
193
194 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 191.193 kb on + strand, within Pf1N1B4_149 at 191.193 kb on + strand, within Pf1N1B4_149 at 191.194 kb on - strand, within Pf1N1B4_149 at 191.194 kb on - strand, within Pf1N1B4_149 at 191.584 kb on - strand at 191.584 kb on - strand at 191.637 kb on + strand at 191.637 kb on + strand at 191.637 kb on + strand at 191.638 kb on - strand at 191.883 kb on + strand at 191.906 kb on - strand at 192.305 kb on - strand, within Pf1N1B4_150 at 192.598 kb on + strand at 192.653 kb on - strand, within Pf1N1B4_150 at 192.653 kb on - strand, within Pf1N1B4_150 at 192.712 kb on + strand, within Pf1N1B4_150 at 192.713 kb on - strand, within Pf1N1B4_150 at 192.746 kb on - strand, within Pf1N1B4_150 at 192.746 kb on - strand, within Pf1N1B4_150 at 192.746 kb on - strand, within Pf1N1B4_150 at 192.848 kb on - strand, within Pf1N1B4_150 at 192.848 kb on - strand, within Pf1N1B4_150 at 193.111 kb on - strand at 193.190 kb on + strand at 193.813 kb on + strand at 193.813 kb on + strand at 193.875 kb on + strand at 193.894 kb on + strand at 193.895 kb on - strand at 193.925 kb on + strand at 193.959 kb on + strand at 193.960 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Lactate (C) remove 191,193 + Pf1N1B4_149 0.82 -0.3 191,193 + Pf1N1B4_149 0.82 +0.1 191,194 - Pf1N1B4_149 0.82 -1.1 191,194 - Pf1N1B4_149 0.82 -0.8 191,584 - -0.5 191,584 - -0.2 191,637 + -2.1 191,637 + -0.6 191,637 + +1.5 191,638 - +0.1 191,883 + +0.4 191,906 - -0.0 192,305 - Pf1N1B4_150 0.21 -0.5 192,598 + +1.1 192,653 - Pf1N1B4_150 0.56 +0.1 192,653 - Pf1N1B4_150 0.56 -1.2 192,712 + Pf1N1B4_150 0.62 +0.7 192,713 - Pf1N1B4_150 0.62 -0.8 192,746 - Pf1N1B4_150 0.65 -1.2 192,746 - Pf1N1B4_150 0.65 -0.3 192,746 - Pf1N1B4_150 0.65 -3.1 192,848 - Pf1N1B4_150 0.76 -1.8 192,848 - Pf1N1B4_150 0.76 -0.2 193,111 - +0.1 193,190 + -0.1 193,813 + -0.7 193,813 + +0.7 193,875 + +0.4 193,894 + +2.1 193,895 - -0.4 193,925 + -0.9 193,959 + +0.8 193,960 - +0.1
Or see this region's nucleotide sequence