Experiment: Dextran 5 mg/ml (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS05340 and HMPREF1058_RS20985 are separated by 286 nucleotides HMPREF1058_RS20985 and HMPREF1058_RS05335 are separated by 294 nucleotides HMPREF1058_RS05335 and HMPREF1058_RS05330 are separated by 91 nucleotides HMPREF1058_RS05330 and HMPREF1058_RS05325 overlap by 16 nucleotides
HMPREF1058_RS05340: HMPREF1058_RS05340 - site-specific integrase, at 186,216 to 187,445
_RS05340
HMPREF1058_RS20985: HMPREF1058_RS20985 - tyrosine-type recombinase/integrase, at 187,732 to 187,899
_RS20985
HMPREF1058_RS05335: HMPREF1058_RS05335 - hypothetical protein, at 188,194 to 188,982
_RS05335
HMPREF1058_RS05330: HMPREF1058_RS05330 - helix-turn-helix domain-containing protein, at 189,074 to 189,448
_RS05330
HMPREF1058_RS05325: HMPREF1058_RS05325 - hypothetical protein, at 189,433 to 190,656
_RS05325
Position (kb) 188
189 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 187.523 kb on + strand at 187.524 kb on - strand at 187.753 kb on + strand, within HMPREF1058_RS20985 at 187.753 kb on + strand, within HMPREF1058_RS20985 at 187.754 kb on - strand, within HMPREF1058_RS20985 at 187.850 kb on + strand, within HMPREF1058_RS20985 at 187.887 kb on - strand at 187.900 kb on + strand at 187.958 kb on + strand at 188.002 kb on + strand at 188.006 kb on + strand at 188.085 kb on - strand at 188.096 kb on - strand at 188.096 kb on - strand at 188.100 kb on + strand at 188.228 kb on + strand at 188.228 kb on + strand at 188.228 kb on + strand at 188.231 kb on - strand at 188.317 kb on + strand, within HMPREF1058_RS05335 at 188.318 kb on - strand, within HMPREF1058_RS05335 at 188.318 kb on - strand, within HMPREF1058_RS05335 at 188.344 kb on - strand, within HMPREF1058_RS05335 at 188.348 kb on + strand, within HMPREF1058_RS05335 at 188.421 kb on - strand, within HMPREF1058_RS05335 at 188.437 kb on - strand, within HMPREF1058_RS05335 at 188.489 kb on + strand, within HMPREF1058_RS05335 at 188.541 kb on + strand, within HMPREF1058_RS05335 at 188.567 kb on + strand, within HMPREF1058_RS05335 at 188.612 kb on + strand, within HMPREF1058_RS05335 at 188.612 kb on + strand, within HMPREF1058_RS05335 at 188.623 kb on - strand, within HMPREF1058_RS05335 at 188.640 kb on + strand, within HMPREF1058_RS05335 at 188.652 kb on + strand, within HMPREF1058_RS05335 at 188.721 kb on - strand, within HMPREF1058_RS05335 at 188.777 kb on + strand, within HMPREF1058_RS05335 at 188.787 kb on + strand, within HMPREF1058_RS05335 at 188.873 kb on + strand, within HMPREF1058_RS05335 at 188.874 kb on - strand, within HMPREF1058_RS05335 at 188.997 kb on + strand at 189.016 kb on + strand at 189.038 kb on - strand at 189.040 kb on + strand at 189.070 kb on + strand at 189.180 kb on + strand, within HMPREF1058_RS05330 at 189.181 kb on - strand, within HMPREF1058_RS05330 at 189.250 kb on + strand, within HMPREF1058_RS05330 at 189.264 kb on - strand, within HMPREF1058_RS05330 at 189.297 kb on - strand, within HMPREF1058_RS05330 at 189.297 kb on - strand, within HMPREF1058_RS05330 at 189.297 kb on - strand, within HMPREF1058_RS05330 at 189.390 kb on - strand, within HMPREF1058_RS05330 at 189.399 kb on + strand, within HMPREF1058_RS05330 at 189.418 kb on + strand at 189.478 kb on + strand at 189.482 kb on + strand at 189.483 kb on - strand at 189.503 kb on - strand at 189.503 kb on - strand at 189.550 kb on - strand at 189.575 kb on + strand, within HMPREF1058_RS05325 at 189.576 kb on - strand, within HMPREF1058_RS05325 at 189.603 kb on - strand, within HMPREF1058_RS05325 at 189.620 kb on - strand, within HMPREF1058_RS05325 at 189.620 kb on - strand, within HMPREF1058_RS05325 at 189.651 kb on - strand, within HMPREF1058_RS05325 at 189.705 kb on + strand, within HMPREF1058_RS05325 at 189.732 kb on + strand, within HMPREF1058_RS05325 at 189.733 kb on - strand, within HMPREF1058_RS05325 at 189.735 kb on - strand, within HMPREF1058_RS05325 at 189.745 kb on - strand, within HMPREF1058_RS05325 at 189.769 kb on + strand, within HMPREF1058_RS05325 at 189.777 kb on - strand, within HMPREF1058_RS05325 at 189.787 kb on + strand, within HMPREF1058_RS05325 at 189.834 kb on - strand, within HMPREF1058_RS05325 at 189.864 kb on + strand, within HMPREF1058_RS05325 at 189.864 kb on + strand, within HMPREF1058_RS05325 at 189.912 kb on + strand, within HMPREF1058_RS05325 at 189.972 kb on - strand, within HMPREF1058_RS05325
Per-strain Table
Position Strand Gene LocusTag Fraction Dextran 5 mg/ml (C) remove 187,523 + -1.0 187,524 - -0.7 187,753 + HMPREF1058_RS20985 0.12 -2.0 187,753 + HMPREF1058_RS20985 0.12 -0.8 187,754 - HMPREF1058_RS20985 0.13 -0.1 187,850 + HMPREF1058_RS20985 0.70 -0.7 187,887 - +0.1 187,900 + -2.0 187,958 + +0.7 188,002 + +0.2 188,006 + +0.1 188,085 - -0.6 188,096 - +1.3 188,096 - -2.3 188,100 + -0.7 188,228 + -3.5 188,228 + +1.4 188,228 + +3.2 188,231 - -2.7 188,317 + HMPREF1058_RS05335 0.16 +1.2 188,318 - HMPREF1058_RS05335 0.16 -0.7 188,318 - HMPREF1058_RS05335 0.16 +0.0 188,344 - HMPREF1058_RS05335 0.19 -0.7 188,348 + HMPREF1058_RS05335 0.20 +0.2 188,421 - HMPREF1058_RS05335 0.29 -1.7 188,437 - HMPREF1058_RS05335 0.31 +0.0 188,489 + HMPREF1058_RS05335 0.37 +0.6 188,541 + HMPREF1058_RS05335 0.44 +0.1 188,567 + HMPREF1058_RS05335 0.47 -0.4 188,612 + HMPREF1058_RS05335 0.53 -0.3 188,612 + HMPREF1058_RS05335 0.53 -0.7 188,623 - HMPREF1058_RS05335 0.54 -0.3 188,640 + HMPREF1058_RS05335 0.57 -1.0 188,652 + HMPREF1058_RS05335 0.58 -1.0 188,721 - HMPREF1058_RS05335 0.67 +0.0 188,777 + HMPREF1058_RS05335 0.74 +1.3 188,787 + HMPREF1058_RS05335 0.75 +0.1 188,873 + HMPREF1058_RS05335 0.86 -0.1 188,874 - HMPREF1058_RS05335 0.86 -1.2 188,997 + -0.6 189,016 + -1.2 189,038 - -0.1 189,040 + +0.1 189,070 + +0.7 189,180 + HMPREF1058_RS05330 0.28 -1.2 189,181 - HMPREF1058_RS05330 0.29 +1.7 189,250 + HMPREF1058_RS05330 0.47 -1.2 189,264 - HMPREF1058_RS05330 0.51 -1.2 189,297 - HMPREF1058_RS05330 0.59 -2.7 189,297 - HMPREF1058_RS05330 0.59 -1.1 189,297 - HMPREF1058_RS05330 0.59 -0.6 189,390 - HMPREF1058_RS05330 0.84 -0.0 189,399 + HMPREF1058_RS05330 0.87 -0.0 189,418 + -1.3 189,478 + -0.1 189,482 + +1.3 189,483 - -1.0 189,503 - +1.9 189,503 - +0.1 189,550 - -3.2 189,575 + HMPREF1058_RS05325 0.12 -0.7 189,576 - HMPREF1058_RS05325 0.12 -0.6 189,603 - HMPREF1058_RS05325 0.14 +0.3 189,620 - HMPREF1058_RS05325 0.15 +0.2 189,620 - HMPREF1058_RS05325 0.15 -1.7 189,651 - HMPREF1058_RS05325 0.18 -1.2 189,705 + HMPREF1058_RS05325 0.22 +0.5 189,732 + HMPREF1058_RS05325 0.24 -2.4 189,733 - HMPREF1058_RS05325 0.25 +0.3 189,735 - HMPREF1058_RS05325 0.25 +0.5 189,745 - HMPREF1058_RS05325 0.25 +0.0 189,769 + HMPREF1058_RS05325 0.27 -0.8 189,777 - HMPREF1058_RS05325 0.28 -0.2 189,787 + HMPREF1058_RS05325 0.29 -1.9 189,834 - HMPREF1058_RS05325 0.33 +2.7 189,864 + HMPREF1058_RS05325 0.35 +0.1 189,864 + HMPREF1058_RS05325 0.35 -0.3 189,912 + HMPREF1058_RS05325 0.39 +0.7 189,972 - HMPREF1058_RS05325 0.44 -0.9
Or see this region's nucleotide sequence