Experiment: R2A with Chloride 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS15715 and CA265_RS15720 are separated by 90 nucleotides CA265_RS15720 and CA265_RS15725 are separated by 113 nucleotides CA265_RS15725 and CA265_RS15730 are separated by 103 nucleotides CA265_RS15730 and CA265_RS15735 are separated by 89 nucleotides
CA265_RS15715: CA265_RS15715 - hypothetical protein, at 3,962,294 to 3,963,547
_RS15715
CA265_RS15720: CA265_RS15720 - hypothetical protein, at 3,963,638 to 3,964,018
_RS15720
CA265_RS15725: CA265_RS15725 - hypothetical protein, at 3,964,132 to 3,964,497
_RS15725
CA265_RS15730: CA265_RS15730 - hypothetical protein, at 3,964,601 to 3,965,347
_RS15730
CA265_RS15735: CA265_RS15735 - GTPase Obg, at 3,965,437 to 3,966,432
_RS15735
Position (kb)
3964
3965 Strain fitness (log2 ratio)
-2
-1
0
1 at 3963.197 kb on + strand, within CA265_RS15715 at 3963.245 kb on - strand, within CA265_RS15715 at 3963.298 kb on + strand, within CA265_RS15715 at 3963.444 kb on + strand at 3963.444 kb on + strand at 3963.445 kb on - strand at 3963.475 kb on + strand at 3963.534 kb on - strand at 3963.592 kb on + strand at 3963.611 kb on + strand at 3963.612 kb on - strand at 3963.613 kb on + strand at 3963.614 kb on - strand at 3963.736 kb on + strand, within CA265_RS15720 at 3963.800 kb on + strand, within CA265_RS15720 at 3963.846 kb on + strand, within CA265_RS15720 at 3963.847 kb on - strand, within CA265_RS15720 at 3963.859 kb on + strand, within CA265_RS15720 at 3963.860 kb on - strand, within CA265_RS15720 at 3963.891 kb on - strand, within CA265_RS15720 at 3963.917 kb on - strand at 3963.987 kb on + strand at 3963.987 kb on + strand at 3964.097 kb on - strand at 3964.126 kb on + strand at 3964.194 kb on - strand, within CA265_RS15725 at 3964.232 kb on + strand, within CA265_RS15725 at 3964.237 kb on - strand, within CA265_RS15725 at 3964.237 kb on - strand, within CA265_RS15725 at 3964.327 kb on + strand, within CA265_RS15725 at 3964.358 kb on + strand, within CA265_RS15725 at 3964.377 kb on + strand, within CA265_RS15725 at 3964.378 kb on - strand, within CA265_RS15725 at 3964.378 kb on - strand, within CA265_RS15725 at 3964.378 kb on - strand, within CA265_RS15725 at 3964.410 kb on + strand, within CA265_RS15725 at 3964.410 kb on + strand, within CA265_RS15725 at 3964.411 kb on - strand, within CA265_RS15725 at 3964.501 kb on + strand at 3964.599 kb on - strand at 3964.712 kb on + strand, within CA265_RS15730 at 3964.716 kb on + strand, within CA265_RS15730 at 3964.785 kb on - strand, within CA265_RS15730 at 3964.827 kb on + strand, within CA265_RS15730 at 3964.922 kb on - strand, within CA265_RS15730 at 3964.951 kb on + strand, within CA265_RS15730 at 3964.987 kb on - strand, within CA265_RS15730 at 3964.987 kb on - strand, within CA265_RS15730 at 3965.012 kb on + strand, within CA265_RS15730 at 3965.090 kb on + strand, within CA265_RS15730 at 3965.090 kb on + strand, within CA265_RS15730 at 3965.090 kb on + strand, within CA265_RS15730 at 3965.090 kb on + strand, within CA265_RS15730 at 3965.091 kb on - strand, within CA265_RS15730 at 3965.091 kb on - strand, within CA265_RS15730 at 3965.091 kb on - strand, within CA265_RS15730 at 3965.093 kb on - strand, within CA265_RS15730 at 3965.251 kb on - strand, within CA265_RS15730 at 3965.328 kb on + strand at 3965.345 kb on + strand at 3965.349 kb on + strand at 3965.357 kb on + strand at 3965.358 kb on - strand at 3965.363 kb on - strand at 3965.363 kb on - strand at 3965.419 kb on - strand at 3965.439 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloride 300 mM remove 3,963,197 + CA265_RS15715 0.72 -0.5 3,963,245 - CA265_RS15715 0.76 -0.8 3,963,298 + CA265_RS15715 0.80 +1.2 3,963,444 + -0.5 3,963,444 + +0.0 3,963,445 - +0.6 3,963,475 + +0.0 3,963,534 - +1.2 3,963,592 + -2.1 3,963,611 + -0.1 3,963,612 - -1.6 3,963,613 + -0.5 3,963,614 - -0.2 3,963,736 + CA265_RS15720 0.26 -1.0 3,963,800 + CA265_RS15720 0.43 -0.7 3,963,846 + CA265_RS15720 0.55 +1.1 3,963,847 - CA265_RS15720 0.55 -1.8 3,963,859 + CA265_RS15720 0.58 -1.1 3,963,860 - CA265_RS15720 0.58 -0.2 3,963,891 - CA265_RS15720 0.66 -0.7 3,963,917 - -0.0 3,963,987 + -0.2 3,963,987 + -0.0 3,964,097 - -0.3 3,964,126 + -0.1 3,964,194 - CA265_RS15725 0.17 -0.2 3,964,232 + CA265_RS15725 0.27 +1.6 3,964,237 - CA265_RS15725 0.29 -0.6 3,964,237 - CA265_RS15725 0.29 -0.2 3,964,327 + CA265_RS15725 0.53 +0.2 3,964,358 + CA265_RS15725 0.62 -1.4 3,964,377 + CA265_RS15725 0.67 -0.8 3,964,378 - CA265_RS15725 0.67 +0.7 3,964,378 - CA265_RS15725 0.67 -0.4 3,964,378 - CA265_RS15725 0.67 +0.2 3,964,410 + CA265_RS15725 0.76 +0.2 3,964,410 + CA265_RS15725 0.76 +0.0 3,964,411 - CA265_RS15725 0.76 -0.8 3,964,501 + -1.0 3,964,599 - -0.3 3,964,712 + CA265_RS15730 0.15 -0.5 3,964,716 + CA265_RS15730 0.15 +0.0 3,964,785 - CA265_RS15730 0.25 -0.8 3,964,827 + CA265_RS15730 0.30 -0.4 3,964,922 - CA265_RS15730 0.43 -1.4 3,964,951 + CA265_RS15730 0.47 -0.3 3,964,987 - CA265_RS15730 0.52 +0.6 3,964,987 - CA265_RS15730 0.52 +0.6 3,965,012 + CA265_RS15730 0.55 -0.5 3,965,090 + CA265_RS15730 0.65 -0.2 3,965,090 + CA265_RS15730 0.65 -1.6 3,965,090 + CA265_RS15730 0.65 -0.0 3,965,090 + CA265_RS15730 0.65 +0.4 3,965,091 - CA265_RS15730 0.66 -0.1 3,965,091 - CA265_RS15730 0.66 -0.2 3,965,091 - CA265_RS15730 0.66 +0.3 3,965,093 - CA265_RS15730 0.66 +1.1 3,965,251 - CA265_RS15730 0.87 -1.0 3,965,328 + -1.4 3,965,345 + +0.3 3,965,349 + -1.1 3,965,357 + -0.4 3,965,358 - +0.1 3,965,363 - +0.5 3,965,363 - +0.3 3,965,419 - -0.4 3,965,439 - +1.1
Or see this region's nucleotide sequence