Experiment: R2A with Chloride 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS04150 and CA265_RS04155 are separated by 271 nucleotides CA265_RS04155 and CA265_RS04160 are separated by 84 nucleotides
CA265_RS04150: CA265_RS04150 - glycosyl hydrolase, at 999,721 to 1,001,505
_RS04150
CA265_RS04155: CA265_RS04155 - bifunctional alpha,alpha-trehalose-phosphate synthase (UDP-forming)/trehalose-phosphatase, at 1,001,777 to 1,003,966
_RS04155
CA265_RS04160: CA265_RS04160 - response regulator receiver protein, at 1,004,051 to 1,005,403
_RS04160
Position (kb)
1001
1002
1003
1004 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1000.778 kb on + strand, within CA265_RS04150 at 1000.787 kb on - strand, within CA265_RS04150 at 1000.920 kb on - strand, within CA265_RS04150 at 1000.920 kb on - strand, within CA265_RS04150 at 1000.920 kb on - strand, within CA265_RS04150 at 1001.045 kb on + strand, within CA265_RS04150 at 1001.046 kb on - strand, within CA265_RS04150 at 1001.046 kb on - strand, within CA265_RS04150 at 1001.178 kb on - strand, within CA265_RS04150 at 1001.232 kb on - strand, within CA265_RS04150 at 1001.350 kb on - strand at 1001.352 kb on + strand at 1001.353 kb on - strand at 1001.353 kb on - strand at 1001.356 kb on + strand at 1001.434 kb on + strand at 1001.478 kb on + strand at 1001.495 kb on + strand at 1001.511 kb on + strand at 1001.512 kb on - strand at 1001.561 kb on + strand at 1001.612 kb on + strand at 1001.614 kb on + strand at 1001.617 kb on - strand at 1001.620 kb on + strand at 1001.621 kb on - strand at 1001.621 kb on - strand at 1001.685 kb on + strand at 1001.685 kb on + strand at 1001.733 kb on - strand at 1001.851 kb on - strand at 1001.853 kb on + strand at 1001.890 kb on + strand at 1001.922 kb on + strand at 1001.976 kb on + strand at 1001.977 kb on - strand at 1001.977 kb on - strand at 1002.064 kb on + strand, within CA265_RS04155 at 1002.064 kb on + strand, within CA265_RS04155 at 1002.159 kb on + strand, within CA265_RS04155 at 1002.159 kb on + strand, within CA265_RS04155 at 1002.320 kb on + strand, within CA265_RS04155 at 1002.321 kb on - strand, within CA265_RS04155 at 1002.368 kb on - strand, within CA265_RS04155 at 1002.368 kb on - strand, within CA265_RS04155 at 1002.398 kb on + strand, within CA265_RS04155 at 1002.410 kb on + strand, within CA265_RS04155 at 1002.411 kb on - strand, within CA265_RS04155 at 1002.419 kb on + strand, within CA265_RS04155 at 1002.420 kb on - strand, within CA265_RS04155 at 1002.481 kb on - strand, within CA265_RS04155 at 1002.491 kb on + strand, within CA265_RS04155 at 1002.492 kb on - strand, within CA265_RS04155 at 1002.530 kb on + strand, within CA265_RS04155 at 1002.531 kb on - strand, within CA265_RS04155 at 1002.531 kb on - strand, within CA265_RS04155 at 1002.629 kb on - strand, within CA265_RS04155 at 1002.629 kb on - strand, within CA265_RS04155 at 1002.667 kb on + strand, within CA265_RS04155 at 1002.697 kb on + strand, within CA265_RS04155 at 1002.708 kb on - strand, within CA265_RS04155 at 1002.751 kb on + strand, within CA265_RS04155 at 1002.834 kb on + strand, within CA265_RS04155 at 1002.887 kb on - strand at 1003.084 kb on + strand, within CA265_RS04155 at 1003.085 kb on - strand, within CA265_RS04155 at 1003.087 kb on - strand, within CA265_RS04155 at 1003.149 kb on + strand, within CA265_RS04155 at 1003.175 kb on + strand, within CA265_RS04155 at 1003.242 kb on - strand, within CA265_RS04155 at 1003.316 kb on + strand, within CA265_RS04155 at 1003.317 kb on - strand, within CA265_RS04155 at 1003.317 kb on - strand, within CA265_RS04155 at 1003.343 kb on + strand, within CA265_RS04155 at 1003.343 kb on + strand, within CA265_RS04155 at 1003.363 kb on + strand, within CA265_RS04155 at 1003.387 kb on + strand, within CA265_RS04155 at 1003.425 kb on - strand, within CA265_RS04155 at 1003.479 kb on - strand, within CA265_RS04155 at 1003.479 kb on - strand, within CA265_RS04155 at 1003.479 kb on - strand, within CA265_RS04155 at 1003.480 kb on + strand, within CA265_RS04155 at 1003.481 kb on - strand, within CA265_RS04155 at 1003.481 kb on - strand, within CA265_RS04155 at 1003.481 kb on - strand, within CA265_RS04155 at 1003.481 kb on - strand, within CA265_RS04155 at 1003.545 kb on - strand, within CA265_RS04155 at 1003.545 kb on - strand, within CA265_RS04155 at 1003.692 kb on - strand, within CA265_RS04155 at 1003.717 kb on - strand, within CA265_RS04155 at 1003.717 kb on - strand, within CA265_RS04155 at 1003.763 kb on + strand at 1003.852 kb on + strand at 1003.905 kb on + strand at 1003.905 kb on + strand at 1003.953 kb on - strand at 1003.955 kb on - strand at 1003.972 kb on + strand at 1003.981 kb on + strand at 1003.993 kb on + strand at 1003.993 kb on + strand at 1003.994 kb on - strand at 1004.058 kb on + strand at 1004.066 kb on + strand at 1004.111 kb on + strand at 1004.112 kb on - strand at 1004.138 kb on - strand at 1004.159 kb on + strand at 1004.201 kb on + strand, within CA265_RS04160 at 1004.274 kb on - strand, within CA265_RS04160 at 1004.323 kb on - strand, within CA265_RS04160 at 1004.327 kb on - strand, within CA265_RS04160 at 1004.327 kb on - strand, within CA265_RS04160 at 1004.327 kb on - strand, within CA265_RS04160 at 1004.327 kb on - strand, within CA265_RS04160 at 1004.336 kb on - strand, within CA265_RS04160 at 1004.458 kb on + strand, within CA265_RS04160 at 1004.458 kb on + strand, within CA265_RS04160 at 1004.633 kb on + strand, within CA265_RS04160 at 1004.633 kb on + strand, within CA265_RS04160 at 1004.755 kb on + strand, within CA265_RS04160 at 1004.779 kb on + strand, within CA265_RS04160 at 1004.779 kb on + strand, within CA265_RS04160 at 1004.780 kb on - strand, within CA265_RS04160 at 1004.780 kb on - strand, within CA265_RS04160 at 1004.781 kb on + strand, within CA265_RS04160 at 1004.781 kb on + strand, within CA265_RS04160 at 1004.801 kb on + strand, within CA265_RS04160 at 1004.801 kb on + strand, within CA265_RS04160 at 1004.802 kb on - strand, within CA265_RS04160 at 1004.934 kb on + strand, within CA265_RS04160 at 1004.938 kb on + strand, within CA265_RS04160
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloride 300 mM remove 1,000,778 + CA265_RS04150 0.59 +0.8 1,000,787 - CA265_RS04150 0.60 -0.5 1,000,920 - CA265_RS04150 0.67 -0.4 1,000,920 - CA265_RS04150 0.67 +0.0 1,000,920 - CA265_RS04150 0.67 -0.5 1,001,045 + CA265_RS04150 0.74 +0.4 1,001,046 - CA265_RS04150 0.74 -0.8 1,001,046 - CA265_RS04150 0.74 -0.7 1,001,178 - CA265_RS04150 0.82 +0.1 1,001,232 - CA265_RS04150 0.85 -0.2 1,001,350 - +0.4 1,001,352 + -0.8 1,001,353 - +1.0 1,001,353 - +1.0 1,001,356 + +0.8 1,001,434 + -0.7 1,001,478 + -0.9 1,001,495 + -0.4 1,001,511 + -1.6 1,001,512 - +0.5 1,001,561 + +0.4 1,001,612 + -0.9 1,001,614 + -0.3 1,001,617 - -1.3 1,001,620 + +0.1 1,001,621 - +0.4 1,001,621 - +0.1 1,001,685 + -0.6 1,001,685 + -1.1 1,001,733 - -0.9 1,001,851 - -4.4 1,001,853 + -4.4 1,001,890 + -2.8 1,001,922 + -3.6 1,001,976 + -4.0 1,001,977 - -2.8 1,001,977 - -2.5 1,002,064 + CA265_RS04155 0.13 -4.2 1,002,064 + CA265_RS04155 0.13 -4.7 1,002,159 + CA265_RS04155 0.17 -2.4 1,002,159 + CA265_RS04155 0.17 -3.4 1,002,320 + CA265_RS04155 0.25 -3.6 1,002,321 - CA265_RS04155 0.25 -3.8 1,002,368 - CA265_RS04155 0.27 -3.5 1,002,368 - CA265_RS04155 0.27 -2.1 1,002,398 + CA265_RS04155 0.28 -2.3 1,002,410 + CA265_RS04155 0.29 -3.2 1,002,411 - CA265_RS04155 0.29 -3.1 1,002,419 + CA265_RS04155 0.29 -1.4 1,002,420 - CA265_RS04155 0.29 -4.5 1,002,481 - CA265_RS04155 0.32 -2.7 1,002,491 + CA265_RS04155 0.33 -3.3 1,002,492 - CA265_RS04155 0.33 -2.5 1,002,530 + CA265_RS04155 0.34 -3.5 1,002,531 - CA265_RS04155 0.34 -4.9 1,002,531 - CA265_RS04155 0.34 -3.1 1,002,629 - CA265_RS04155 0.39 -0.6 1,002,629 - CA265_RS04155 0.39 -3.0 1,002,667 + CA265_RS04155 0.41 -2.7 1,002,697 + CA265_RS04155 0.42 -4.4 1,002,708 - CA265_RS04155 0.43 -5.7 1,002,751 + CA265_RS04155 0.44 -3.7 1,002,834 + CA265_RS04155 0.48 -3.5 1,002,887 - -0.6 1,003,084 + CA265_RS04155 0.60 -1.9 1,003,085 - CA265_RS04155 0.60 -2.4 1,003,087 - CA265_RS04155 0.60 -1.4 1,003,149 + CA265_RS04155 0.63 -4.4 1,003,175 + CA265_RS04155 0.64 -4.6 1,003,242 - CA265_RS04155 0.67 -2.9 1,003,316 + CA265_RS04155 0.70 -2.2 1,003,317 - CA265_RS04155 0.70 -3.8 1,003,317 - CA265_RS04155 0.70 -1.7 1,003,343 + CA265_RS04155 0.72 -2.9 1,003,343 + CA265_RS04155 0.72 -2.6 1,003,363 + CA265_RS04155 0.72 -3.4 1,003,387 + CA265_RS04155 0.74 -3.6 1,003,425 - CA265_RS04155 0.75 -3.7 1,003,479 - CA265_RS04155 0.78 -2.0 1,003,479 - CA265_RS04155 0.78 -0.6 1,003,479 - CA265_RS04155 0.78 -2.9 1,003,480 + CA265_RS04155 0.78 -3.0 1,003,481 - CA265_RS04155 0.78 -2.1 1,003,481 - CA265_RS04155 0.78 -2.8 1,003,481 - CA265_RS04155 0.78 -3.8 1,003,481 - CA265_RS04155 0.78 -1.7 1,003,545 - CA265_RS04155 0.81 -4.6 1,003,545 - CA265_RS04155 0.81 -3.8 1,003,692 - CA265_RS04155 0.87 -3.2 1,003,717 - CA265_RS04155 0.89 -2.6 1,003,717 - CA265_RS04155 0.89 -2.6 1,003,763 + -3.1 1,003,852 + -2.1 1,003,905 + -2.8 1,003,905 + -2.0 1,003,953 - -1.7 1,003,955 - -3.0 1,003,972 + -1.4 1,003,981 + -0.7 1,003,993 + +0.6 1,003,993 + -1.7 1,003,994 - +0.1 1,004,058 + -0.5 1,004,066 + +0.8 1,004,111 + +0.4 1,004,112 - +0.1 1,004,138 - -0.5 1,004,159 + +0.2 1,004,201 + CA265_RS04160 0.11 -0.7 1,004,274 - CA265_RS04160 0.16 -0.8 1,004,323 - CA265_RS04160 0.20 -0.0 1,004,327 - CA265_RS04160 0.20 -0.7 1,004,327 - CA265_RS04160 0.20 -0.1 1,004,327 - CA265_RS04160 0.20 -0.3 1,004,327 - CA265_RS04160 0.20 +0.1 1,004,336 - CA265_RS04160 0.21 -0.1 1,004,458 + CA265_RS04160 0.30 -0.3 1,004,458 + CA265_RS04160 0.30 -0.5 1,004,633 + CA265_RS04160 0.43 +0.4 1,004,633 + CA265_RS04160 0.43 +0.1 1,004,755 + CA265_RS04160 0.52 +0.6 1,004,779 + CA265_RS04160 0.54 +0.2 1,004,779 + CA265_RS04160 0.54 -0.0 1,004,780 - CA265_RS04160 0.54 -0.0 1,004,780 - CA265_RS04160 0.54 +0.4 1,004,781 + CA265_RS04160 0.54 +0.1 1,004,781 + CA265_RS04160 0.54 +0.4 1,004,801 + CA265_RS04160 0.55 -0.7 1,004,801 + CA265_RS04160 0.55 -1.3 1,004,802 - CA265_RS04160 0.56 -0.9 1,004,934 + CA265_RS04160 0.65 -1.4 1,004,938 + CA265_RS04160 0.66 -1.1
Or see this region's nucleotide sequence