Experiment: Paraquat dichloride 0.032 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS25405 and BPHYT_RS25410 are separated by 358 nucleotides BPHYT_RS25410 and BPHYT_RS25415 are separated by 76 nucleotides BPHYT_RS25415 and BPHYT_RS25420 are separated by 154 nucleotides
BPHYT_RS25405: BPHYT_RS25405 - CoA-transferase, at 1,277,126 to 1,278,196
_RS25405
BPHYT_RS25410: BPHYT_RS25410 - porin, at 1,278,555 to 1,279,670
_RS25410
BPHYT_RS25415: BPHYT_RS25415 - 3-hydroxy-2-methylbutyryl-CoA dehydrogenase, at 1,279,747 to 1,280,493
_RS25415
BPHYT_RS25420: BPHYT_RS25420 - hypothetical protein, at 1,280,648 to 1,280,842
_RS25420
Position (kb)
1278
1279
1280 Strain fitness (log2 ratio)
-2
-1
0
1 at 1277.686 kb on - strand, within BPHYT_RS25405 at 1277.785 kb on - strand, within BPHYT_RS25405 at 1277.850 kb on + strand, within BPHYT_RS25405 at 1278.292 kb on - strand at 1278.560 kb on + strand at 1278.639 kb on + strand at 1278.843 kb on + strand, within BPHYT_RS25410 at 1278.906 kb on + strand, within BPHYT_RS25410 at 1279.037 kb on + strand, within BPHYT_RS25410 at 1279.087 kb on - strand, within BPHYT_RS25410 at 1279.333 kb on - strand, within BPHYT_RS25410 at 1279.565 kb on + strand at 1279.604 kb on + strand at 1279.612 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.032 mg/ml remove 1,277,686 - BPHYT_RS25405 0.52 -0.6 1,277,785 - BPHYT_RS25405 0.62 -0.3 1,277,850 + BPHYT_RS25405 0.68 +0.0 1,278,292 - +0.3 1,278,560 + +0.4 1,278,639 + -0.2 1,278,843 + BPHYT_RS25410 0.26 -2.1 1,278,906 + BPHYT_RS25410 0.31 -1.0 1,279,037 + BPHYT_RS25410 0.43 -2.0 1,279,087 - BPHYT_RS25410 0.48 -0.6 1,279,333 - BPHYT_RS25410 0.70 -0.8 1,279,565 + -0.2 1,279,604 + +0.1 1,279,612 + -0.3
Or see this region's nucleotide sequence