Experiment: Paraquat dichloride 0.032 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS08840 and BPHYT_RS08845 are separated by 145 nucleotides BPHYT_RS08845 and BPHYT_RS08850 overlap by 11 nucleotides BPHYT_RS08850 and BPHYT_RS08855 are separated by 293 nucleotides BPHYT_RS08855 and BPHYT_RS08860 are separated by 51 nucleotides
BPHYT_RS08840: BPHYT_RS08840 - LysR family transcriptional regulator, at 2,003,850 to 2,004,764
_RS08840
BPHYT_RS08845: BPHYT_RS08845 - RNA polymerase sigma factor, at 2,004,910 to 2,005,644
_RS08845
BPHYT_RS08850: BPHYT_RS08850 - transcriptional regulator, at 2,005,634 to 2,006,569
_RS08850
BPHYT_RS08855: BPHYT_RS08855 - 30S ribosomal protein S6, at 2,006,863 to 2,007,237
_RS08855
BPHYT_RS08860: BPHYT_RS08860 - primosomal replication protein N, at 2,007,289 to 2,007,588
_RS08860
Position (kb)
2005
2006
2007 Strain fitness (log2 ratio)
-2
-1
0
1 at 2004.646 kb on - strand, within BPHYT_RS08840 at 2004.700 kb on - strand at 2004.700 kb on - strand at 2004.726 kb on + strand at 2005.013 kb on - strand, within BPHYT_RS08845 at 2005.159 kb on + strand, within BPHYT_RS08845 at 2005.642 kb on + strand at 2006.145 kb on + strand, within BPHYT_RS08850 at 2006.307 kb on - strand, within BPHYT_RS08850 at 2006.369 kb on + strand, within BPHYT_RS08850 at 2006.370 kb on - strand, within BPHYT_RS08850 at 2006.624 kb on + strand at 2006.625 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.032 mg/ml remove 2,004,646 - BPHYT_RS08840 0.87 +0.6 2,004,700 - +1.3 2,004,700 - +0.4 2,004,726 + +0.1 2,005,013 - BPHYT_RS08845 0.14 -0.5 2,005,159 + BPHYT_RS08845 0.34 -0.5 2,005,642 + -2.5 2,006,145 + BPHYT_RS08850 0.55 +0.5 2,006,307 - BPHYT_RS08850 0.72 -2.3 2,006,369 + BPHYT_RS08850 0.79 -2.5 2,006,370 - BPHYT_RS08850 0.79 -2.0 2,006,624 + -0.8 2,006,625 - -0.6
Or see this region's nucleotide sequence