Experiment: D-Glucosamine Hydrochloride (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_516 and Pf6N2E2_517 are separated by 148 nucleotides Pf6N2E2_517 and Pf6N2E2_518 are separated by 31 nucleotides
Pf6N2E2_516 - Inosose isomerase (EC 5.3.99.-), at 585,558 to 586,379
_516
Pf6N2E2_517 - Epi-inositol hydrolase (EC 3.7.1.-) (from data) , at 586,528 to 588,459
_517
Pf6N2E2_518 - Myo-inositol 2-dehydrogenase (EC 1.1.1.18), at 588,491 to 589,501
_518
Position (kb)
586
587
588
589 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 585.553 kb on + strand at 585.553 kb on + strand at 585.553 kb on + strand at 585.554 kb on - strand at 585.554 kb on - strand at 585.554 kb on - strand at 585.554 kb on - strand at 585.554 kb on - strand at 585.554 kb on - strand at 585.554 kb on - strand at 585.554 kb on - strand at 585.727 kb on + strand, within Pf6N2E2_516 at 585.728 kb on - strand, within Pf6N2E2_516 at 585.829 kb on - strand, within Pf6N2E2_516 at 585.969 kb on + strand, within Pf6N2E2_516 at 585.970 kb on - strand, within Pf6N2E2_516 at 586.063 kb on + strand, within Pf6N2E2_516 at 586.063 kb on + strand, within Pf6N2E2_516 at 586.063 kb on + strand, within Pf6N2E2_516 at 586.064 kb on - strand, within Pf6N2E2_516 at 586.279 kb on - strand, within Pf6N2E2_516 at 586.279 kb on - strand, within Pf6N2E2_516 at 586.279 kb on - strand, within Pf6N2E2_516 at 586.441 kb on - strand at 586.441 kb on - strand at 586.585 kb on + strand at 586.585 kb on + strand at 586.586 kb on - strand at 586.780 kb on + strand, within Pf6N2E2_517 at 586.781 kb on - strand, within Pf6N2E2_517 at 586.781 kb on - strand, within Pf6N2E2_517 at 586.868 kb on + strand, within Pf6N2E2_517 at 586.869 kb on - strand at 586.869 kb on - strand, within Pf6N2E2_517 at 586.869 kb on - strand, within Pf6N2E2_517 at 586.885 kb on + strand, within Pf6N2E2_517 at 586.982 kb on - strand, within Pf6N2E2_517 at 587.111 kb on - strand, within Pf6N2E2_517 at 587.126 kb on - strand, within Pf6N2E2_517 at 587.183 kb on + strand, within Pf6N2E2_517 at 587.183 kb on + strand, within Pf6N2E2_517 at 587.512 kb on - strand, within Pf6N2E2_517 at 587.675 kb on - strand, within Pf6N2E2_517 at 587.686 kb on + strand, within Pf6N2E2_517 at 587.847 kb on + strand, within Pf6N2E2_517 at 587.895 kb on + strand, within Pf6N2E2_517 at 587.895 kb on + strand, within Pf6N2E2_517 at 587.895 kb on + strand, within Pf6N2E2_517 at 587.895 kb on + strand, within Pf6N2E2_517 at 587.895 kb on + strand, within Pf6N2E2_517 at 587.896 kb on - strand, within Pf6N2E2_517 at 587.896 kb on - strand, within Pf6N2E2_517 at 587.896 kb on - strand, within Pf6N2E2_517 at 587.896 kb on - strand, within Pf6N2E2_517 at 587.896 kb on - strand, within Pf6N2E2_517 at 587.896 kb on - strand, within Pf6N2E2_517 at 587.896 kb on - strand, within Pf6N2E2_517 at 587.899 kb on + strand at 587.899 kb on + strand, within Pf6N2E2_517 at 587.899 kb on + strand, within Pf6N2E2_517 at 587.900 kb on - strand, within Pf6N2E2_517 at 587.911 kb on + strand, within Pf6N2E2_517 at 587.912 kb on - strand, within Pf6N2E2_517 at 587.933 kb on - strand, within Pf6N2E2_517 at 587.986 kb on + strand, within Pf6N2E2_517 at 588.010 kb on + strand, within Pf6N2E2_517 at 588.010 kb on + strand, within Pf6N2E2_517 at 588.010 kb on + strand, within Pf6N2E2_517 at 588.010 kb on + strand, within Pf6N2E2_517 at 588.010 kb on + strand, within Pf6N2E2_517 at 588.011 kb on - strand, within Pf6N2E2_517 at 588.011 kb on - strand, within Pf6N2E2_517 at 588.011 kb on - strand, within Pf6N2E2_517 at 588.011 kb on - strand, within Pf6N2E2_517 at 588.011 kb on - strand, within Pf6N2E2_517 at 588.227 kb on - strand, within Pf6N2E2_517 at 588.241 kb on + strand, within Pf6N2E2_517 at 588.242 kb on - strand, within Pf6N2E2_517 at 588.242 kb on - strand, within Pf6N2E2_517 at 588.352 kb on + strand at 588.352 kb on + strand at 588.352 kb on + strand at 588.353 kb on - strand at 588.353 kb on - strand at 588.454 kb on + strand at 588.472 kb on + strand at 588.473 kb on - strand at 588.473 kb on - strand at 588.473 kb on - strand at 588.488 kb on + strand at 588.488 kb on + strand at 588.488 kb on + strand at 588.488 kb on + strand at 588.488 kb on + strand at 588.488 kb on + strand at 588.488 kb on + strand at 588.489 kb on - strand at 588.489 kb on - strand at 588.489 kb on - strand at 588.489 kb on - strand at 588.489 kb on - strand at 588.489 kb on - strand at 588.489 kb on - strand at 588.489 kb on - strand at 588.489 kb on - strand at 588.541 kb on + strand at 588.653 kb on + strand, within Pf6N2E2_518 at 588.876 kb on - strand, within Pf6N2E2_518 at 588.876 kb on - strand, within Pf6N2E2_518 at 588.876 kb on - strand, within Pf6N2E2_518 at 588.981 kb on - strand, within Pf6N2E2_518 at 588.981 kb on - strand, within Pf6N2E2_518 at 588.981 kb on - strand, within Pf6N2E2_518 at 589.055 kb on + strand, within Pf6N2E2_518 at 589.055 kb on + strand, within Pf6N2E2_518 at 589.055 kb on + strand, within Pf6N2E2_518 at 589.055 kb on + strand, within Pf6N2E2_518 at 589.055 kb on + strand, within Pf6N2E2_518 at 589.055 kb on + strand, within Pf6N2E2_518 at 589.055 kb on + strand, within Pf6N2E2_518 at 589.055 kb on + strand, within Pf6N2E2_518 at 589.056 kb on - strand, within Pf6N2E2_518 at 589.056 kb on - strand, within Pf6N2E2_518 at 589.056 kb on - strand, within Pf6N2E2_518 at 589.188 kb on - strand, within Pf6N2E2_518 at 589.329 kb on - strand, within Pf6N2E2_518 at 589.329 kb on - strand, within Pf6N2E2_518
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (C) remove 585,553 + -1.7 585,553 + -1.5 585,553 + -0.5 585,554 - -0.1 585,554 - -0.7 585,554 - -1.0 585,554 - +1.7 585,554 - +1.2 585,554 - -1.8 585,554 - -0.3 585,554 - -1.1 585,727 + Pf6N2E2_516 0.21 -0.9 585,728 - Pf6N2E2_516 0.21 +0.6 585,829 - Pf6N2E2_516 0.33 -0.4 585,969 + Pf6N2E2_516 0.50 -1.5 585,970 - Pf6N2E2_516 0.50 -0.8 586,063 + Pf6N2E2_516 0.61 -2.0 586,063 + Pf6N2E2_516 0.61 -2.0 586,063 + Pf6N2E2_516 0.61 -1.9 586,064 - Pf6N2E2_516 0.62 +0.2 586,279 - Pf6N2E2_516 0.88 -1.7 586,279 - Pf6N2E2_516 0.88 +0.5 586,279 - Pf6N2E2_516 0.88 -0.5 586,441 - +1.5 586,441 - -0.5 586,585 + -0.2 586,585 + +0.1 586,586 - -1.8 586,780 + Pf6N2E2_517 0.13 -0.9 586,781 - Pf6N2E2_517 0.13 -2.0 586,781 - Pf6N2E2_517 0.13 -2.0 586,868 + Pf6N2E2_517 0.18 -1.9 586,869 - +1.7 586,869 - Pf6N2E2_517 0.18 -1.7 586,869 - Pf6N2E2_517 0.18 -0.5 586,885 + Pf6N2E2_517 0.18 -0.3 586,982 - Pf6N2E2_517 0.23 -0.4 587,111 - Pf6N2E2_517 0.30 +1.9 587,126 - Pf6N2E2_517 0.31 +0.9 587,183 + Pf6N2E2_517 0.34 -0.6 587,183 + Pf6N2E2_517 0.34 +0.2 587,512 - Pf6N2E2_517 0.51 -0.1 587,675 - Pf6N2E2_517 0.59 +0.2 587,686 + Pf6N2E2_517 0.60 +0.1 587,847 + Pf6N2E2_517 0.68 -0.7 587,895 + Pf6N2E2_517 0.71 -0.8 587,895 + Pf6N2E2_517 0.71 -2.1 587,895 + Pf6N2E2_517 0.71 -1.6 587,895 + Pf6N2E2_517 0.71 -1.9 587,895 + Pf6N2E2_517 0.71 +0.0 587,896 - Pf6N2E2_517 0.71 +0.0 587,896 - Pf6N2E2_517 0.71 -0.3 587,896 - Pf6N2E2_517 0.71 -2.0 587,896 - Pf6N2E2_517 0.71 -0.6 587,896 - Pf6N2E2_517 0.71 -0.5 587,896 - Pf6N2E2_517 0.71 -0.3 587,896 - Pf6N2E2_517 0.71 -0.2 587,899 + +1.7 587,899 + Pf6N2E2_517 0.71 -1.1 587,899 + Pf6N2E2_517 0.71 +1.2 587,900 - Pf6N2E2_517 0.71 +0.3 587,911 + Pf6N2E2_517 0.72 -0.1 587,912 - Pf6N2E2_517 0.72 -0.7 587,933 - Pf6N2E2_517 0.73 -1.5 587,986 + Pf6N2E2_517 0.75 -1.3 588,010 + Pf6N2E2_517 0.77 +0.5 588,010 + Pf6N2E2_517 0.77 -1.6 588,010 + Pf6N2E2_517 0.77 -0.7 588,010 + Pf6N2E2_517 0.77 -0.9 588,010 + Pf6N2E2_517 0.77 -0.4 588,011 - Pf6N2E2_517 0.77 -1.9 588,011 - Pf6N2E2_517 0.77 -0.9 588,011 - Pf6N2E2_517 0.77 -1.4 588,011 - Pf6N2E2_517 0.77 -0.6 588,011 - Pf6N2E2_517 0.77 -1.1 588,227 - Pf6N2E2_517 0.88 -0.5 588,241 + Pf6N2E2_517 0.89 -3.1 588,242 - Pf6N2E2_517 0.89 +0.3 588,242 - Pf6N2E2_517 0.89 -1.0 588,352 + -1.8 588,352 + -3.0 588,352 + -1.8 588,353 - -0.2 588,353 - +1.5 588,454 + -3.4 588,472 + -1.4 588,473 - -0.7 588,473 - -0.3 588,473 - -0.1 588,488 + +0.4 588,488 + +1.1 588,488 + -0.8 588,488 + -1.2 588,488 + +0.4 588,488 + -0.8 588,488 + -0.7 588,489 - +0.5 588,489 - +0.6 588,489 - -0.6 588,489 - -0.0 588,489 - +1.0 588,489 - +0.3 588,489 - -0.7 588,489 - -0.9 588,489 - -0.3 588,541 + -1.2 588,653 + Pf6N2E2_518 0.16 -1.9 588,876 - Pf6N2E2_518 0.38 -0.3 588,876 - Pf6N2E2_518 0.38 -0.4 588,876 - Pf6N2E2_518 0.38 -1.5 588,981 - Pf6N2E2_518 0.48 +1.4 588,981 - Pf6N2E2_518 0.48 -2.2 588,981 - Pf6N2E2_518 0.48 -0.0 589,055 + Pf6N2E2_518 0.56 -1.5 589,055 + Pf6N2E2_518 0.56 -1.0 589,055 + Pf6N2E2_518 0.56 +0.2 589,055 + Pf6N2E2_518 0.56 -1.7 589,055 + Pf6N2E2_518 0.56 -0.7 589,055 + Pf6N2E2_518 0.56 -0.6 589,055 + Pf6N2E2_518 0.56 +0.1 589,055 + Pf6N2E2_518 0.56 -1.5 589,056 - Pf6N2E2_518 0.56 -2.5 589,056 - Pf6N2E2_518 0.56 +0.7 589,056 - Pf6N2E2_518 0.56 -0.3 589,188 - Pf6N2E2_518 0.69 -0.9 589,329 - Pf6N2E2_518 0.83 +0.2 589,329 - Pf6N2E2_518 0.83 +0.8
Or see this region's nucleotide sequence