Experiment: D-Glucosamine Hydrochloride (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4750 and Pf6N2E2_4751 are separated by 252 nucleotides Pf6N2E2_4751 and Pf6N2E2_4752 are separated by 228 nucleotides Pf6N2E2_4752 and Pf6N2E2_4753 are separated by 512 nucleotides
Pf6N2E2_4750 - Predicted signal-transduction protein containing cAMP-binding and CBS domains, at 5,485,968 to 5,487,908
_4750
Pf6N2E2_4751 - response regulator, at 5,488,161 to 5,488,607
_4751
Pf6N2E2_4752 - Malate synthase G (EC 2.3.3.9), at 5,488,836 to 5,491,013
_4752
Pf6N2E2_4753 - Glycine cleavage system transcriptional antiactivator GcvR, at 5,491,526 to 5,492,044
_4753
Position (kb)
5488
5489
5490
5491
5492 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 5487.903 kb on - strand at 5487.909 kb on + strand at 5487.909 kb on + strand at 5487.910 kb on - strand at 5487.987 kb on + strand at 5487.987 kb on + strand at 5487.987 kb on + strand at 5487.987 kb on + strand at 5487.987 kb on + strand at 5487.987 kb on + strand at 5487.987 kb on + strand at 5487.987 kb on + strand at 5487.987 kb on + strand at 5487.987 kb on + strand at 5487.987 kb on + strand at 5487.987 kb on + strand at 5487.987 kb on + strand at 5487.987 kb on + strand at 5487.988 kb on - strand at 5487.988 kb on - strand at 5487.988 kb on - strand at 5487.988 kb on - strand at 5487.988 kb on - strand at 5487.988 kb on - strand at 5487.988 kb on - strand at 5487.988 kb on - strand at 5487.988 kb on - strand at 5487.993 kb on + strand at 5487.994 kb on - strand at 5488.006 kb on - strand at 5488.017 kb on + strand at 5488.018 kb on - strand at 5488.018 kb on - strand at 5488.030 kb on + strand at 5488.057 kb on + strand at 5488.057 kb on + strand at 5488.057 kb on + strand at 5488.057 kb on + strand at 5488.058 kb on - strand at 5488.058 kb on - strand at 5488.058 kb on - strand at 5488.058 kb on - strand at 5488.058 kb on - strand at 5488.141 kb on + strand at 5488.142 kb on - strand at 5488.150 kb on + strand at 5488.150 kb on + strand at 5488.150 kb on + strand at 5488.150 kb on + strand at 5488.150 kb on + strand at 5488.150 kb on + strand at 5488.150 kb on + strand at 5488.150 kb on + strand at 5488.151 kb on - strand at 5488.151 kb on - strand at 5488.175 kb on + strand at 5488.175 kb on + strand at 5488.175 kb on + strand at 5488.176 kb on - strand at 5488.427 kb on + strand, within Pf6N2E2_4751 at 5488.427 kb on + strand, within Pf6N2E2_4751 at 5488.428 kb on - strand, within Pf6N2E2_4751 at 5488.428 kb on - strand, within Pf6N2E2_4751 at 5488.477 kb on + strand, within Pf6N2E2_4751 at 5488.605 kb on + strand at 5488.605 kb on + strand at 5488.695 kb on + strand at 5488.695 kb on + strand at 5488.696 kb on - strand at 5488.772 kb on + strand at 5488.772 kb on + strand at 5488.772 kb on + strand at 5488.772 kb on + strand at 5488.772 kb on + strand at 5488.772 kb on + strand at 5488.773 kb on - strand at 5488.773 kb on - strand at 5488.894 kb on + strand at 5488.895 kb on - strand at 5488.984 kb on + strand at 5488.985 kb on - strand at 5488.985 kb on - strand at 5489.105 kb on + strand, within Pf6N2E2_4752 at 5489.105 kb on + strand, within Pf6N2E2_4752 at 5489.105 kb on + strand, within Pf6N2E2_4752 at 5489.105 kb on + strand, within Pf6N2E2_4752 at 5489.105 kb on + strand, within Pf6N2E2_4752 at 5489.106 kb on - strand, within Pf6N2E2_4752 at 5489.197 kb on + strand, within Pf6N2E2_4752 at 5489.197 kb on + strand, within Pf6N2E2_4752 at 5489.197 kb on + strand, within Pf6N2E2_4752 at 5489.197 kb on + strand, within Pf6N2E2_4752 at 5489.356 kb on + strand, within Pf6N2E2_4752 at 5489.545 kb on + strand, within Pf6N2E2_4752 at 5489.546 kb on - strand, within Pf6N2E2_4752 at 5489.546 kb on - strand, within Pf6N2E2_4752 at 5489.744 kb on + strand, within Pf6N2E2_4752 at 5489.763 kb on + strand, within Pf6N2E2_4752 at 5489.763 kb on + strand, within Pf6N2E2_4752 at 5489.763 kb on + strand, within Pf6N2E2_4752 at 5489.764 kb on - strand, within Pf6N2E2_4752 at 5489.764 kb on - strand, within Pf6N2E2_4752 at 5489.764 kb on - strand, within Pf6N2E2_4752 at 5489.764 kb on - strand, within Pf6N2E2_4752 at 5489.764 kb on - strand, within Pf6N2E2_4752 at 5489.764 kb on - strand, within Pf6N2E2_4752 at 5489.839 kb on + strand, within Pf6N2E2_4752 at 5489.839 kb on + strand, within Pf6N2E2_4752 at 5489.839 kb on + strand, within Pf6N2E2_4752 at 5489.839 kb on + strand, within Pf6N2E2_4752 at 5489.839 kb on + strand, within Pf6N2E2_4752 at 5489.839 kb on + strand, within Pf6N2E2_4752 at 5489.840 kb on - strand, within Pf6N2E2_4752 at 5489.927 kb on + strand, within Pf6N2E2_4752 at 5489.927 kb on + strand, within Pf6N2E2_4752 at 5489.928 kb on - strand, within Pf6N2E2_4752 at 5489.928 kb on - strand, within Pf6N2E2_4752 at 5489.928 kb on - strand, within Pf6N2E2_4752 at 5489.928 kb on - strand, within Pf6N2E2_4752 at 5490.052 kb on + strand, within Pf6N2E2_4752 at 5490.052 kb on + strand, within Pf6N2E2_4752 at 5490.149 kb on - strand, within Pf6N2E2_4752 at 5490.149 kb on - strand, within Pf6N2E2_4752 at 5490.436 kb on + strand, within Pf6N2E2_4752 at 5490.437 kb on - strand, within Pf6N2E2_4752 at 5490.586 kb on + strand, within Pf6N2E2_4752 at 5490.587 kb on - strand, within Pf6N2E2_4752 at 5490.587 kb on - strand, within Pf6N2E2_4752 at 5490.587 kb on - strand, within Pf6N2E2_4752 at 5490.598 kb on + strand, within Pf6N2E2_4752 at 5490.599 kb on - strand, within Pf6N2E2_4752 at 5490.599 kb on - strand, within Pf6N2E2_4752 at 5490.713 kb on + strand, within Pf6N2E2_4752 at 5490.714 kb on - strand, within Pf6N2E2_4752 at 5490.745 kb on + strand, within Pf6N2E2_4752 at 5490.746 kb on - strand, within Pf6N2E2_4752 at 5490.746 kb on - strand, within Pf6N2E2_4752 at 5490.772 kb on + strand, within Pf6N2E2_4752 at 5490.772 kb on + strand, within Pf6N2E2_4752 at 5490.772 kb on + strand, within Pf6N2E2_4752 at 5490.773 kb on - strand, within Pf6N2E2_4752 at 5490.856 kb on - strand at 5491.036 kb on + strand at 5491.036 kb on + strand at 5491.036 kb on + strand at 5491.037 kb on - strand at 5491.085 kb on + strand at 5491.085 kb on + strand at 5491.085 kb on + strand at 5491.086 kb on - strand at 5491.086 kb on - strand at 5491.086 kb on - strand at 5491.086 kb on - strand at 5491.086 kb on - strand at 5491.086 kb on - strand at 5491.109 kb on - strand at 5491.109 kb on - strand at 5491.109 kb on - strand at 5491.109 kb on - strand at 5491.135 kb on + strand at 5491.135 kb on + strand at 5491.135 kb on + strand at 5491.136 kb on - strand at 5491.178 kb on - strand at 5491.191 kb on + strand at 5491.191 kb on + strand at 5491.191 kb on + strand at 5491.191 kb on + strand at 5491.192 kb on - strand at 5491.192 kb on - strand at 5491.192 kb on - strand at 5491.192 kb on - strand at 5491.199 kb on + strand at 5491.199 kb on + strand at 5491.199 kb on + strand at 5491.199 kb on + strand at 5491.199 kb on + strand at 5491.200 kb on - strand at 5491.200 kb on - strand at 5491.200 kb on - strand at 5491.200 kb on - strand at 5491.200 kb on - strand at 5491.200 kb on - strand at 5491.200 kb on - strand at 5491.200 kb on - strand at 5491.200 kb on - strand at 5491.200 kb on - strand at 5491.200 kb on - strand at 5491.203 kb on + strand at 5491.203 kb on + strand at 5491.203 kb on + strand at 5491.204 kb on - strand at 5491.204 kb on - strand at 5491.220 kb on + strand at 5491.221 kb on - strand at 5491.221 kb on - strand at 5491.221 kb on - strand at 5491.221 kb on - strand at 5491.223 kb on + strand at 5491.223 kb on + strand at 5491.223 kb on + strand at 5491.223 kb on + strand at 5491.223 kb on + strand at 5491.223 kb on + strand at 5491.223 kb on + strand at 5491.223 kb on + strand at 5491.223 kb on + strand at 5491.223 kb on + strand at 5491.224 kb on - strand at 5491.224 kb on - strand at 5491.224 kb on - strand at 5491.224 kb on - strand at 5491.224 kb on - strand at 5491.224 kb on - strand at 5491.224 kb on - strand at 5491.224 kb on - strand at 5491.224 kb on - strand at 5491.224 kb on - strand at 5491.224 kb on - strand at 5491.255 kb on + strand at 5491.255 kb on + strand at 5491.255 kb on + strand at 5491.255 kb on + strand at 5491.255 kb on + strand at 5491.255 kb on + strand at 5491.256 kb on - strand at 5491.256 kb on - strand at 5491.280 kb on - strand at 5491.287 kb on + strand at 5491.288 kb on - strand at 5491.423 kb on + strand at 5491.492 kb on + strand at 5491.492 kb on + strand at 5491.492 kb on + strand at 5491.492 kb on + strand at 5491.492 kb on + strand at 5491.492 kb on + strand at 5491.492 kb on + strand at 5491.493 kb on - strand at 5491.493 kb on - strand at 5491.493 kb on - strand at 5491.493 kb on - strand at 5491.493 kb on - strand at 5491.493 kb on - strand at 5491.493 kb on - strand at 5491.493 kb on - strand at 5491.494 kb on + strand at 5491.495 kb on - strand at 5491.495 kb on - strand at 5491.495 kb on - strand at 5491.495 kb on - strand at 5491.495 kb on - strand at 5491.495 kb on - strand at 5491.495 kb on - strand at 5491.495 kb on - strand at 5491.730 kb on + strand, within Pf6N2E2_4753 at 5491.730 kb on + strand, within Pf6N2E2_4753 at 5491.731 kb on - strand, within Pf6N2E2_4753 at 5491.731 kb on - strand, within Pf6N2E2_4753 at 5491.731 kb on - strand, within Pf6N2E2_4753 at 5491.731 kb on - strand, within Pf6N2E2_4753 at 5491.827 kb on + strand, within Pf6N2E2_4753 at 5491.828 kb on - strand, within Pf6N2E2_4753 at 5491.889 kb on + strand, within Pf6N2E2_4753 at 5491.889 kb on + strand, within Pf6N2E2_4753 at 5491.889 kb on + strand, within Pf6N2E2_4753 at 5491.889 kb on + strand, within Pf6N2E2_4753 at 5491.889 kb on + strand, within Pf6N2E2_4753 at 5491.889 kb on + strand, within Pf6N2E2_4753 at 5491.890 kb on - strand, within Pf6N2E2_4753 at 5491.890 kb on - strand, within Pf6N2E2_4753 at 5491.890 kb on - strand, within Pf6N2E2_4753 at 5491.890 kb on - strand, within Pf6N2E2_4753 at 5491.890 kb on - strand, within Pf6N2E2_4753
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (C) remove 5,487,903 - +1.2 5,487,909 + +0.9 5,487,909 + +0.7 5,487,910 - -0.6 5,487,987 + +0.8 5,487,987 + +0.6 5,487,987 + -1.5 5,487,987 + +0.6 5,487,987 + +0.1 5,487,987 + -0.1 5,487,987 + -1.8 5,487,987 + -0.1 5,487,987 + -0.0 5,487,987 + -0.1 5,487,987 + -0.6 5,487,987 + +0.5 5,487,987 + +0.7 5,487,987 + +0.4 5,487,988 - +0.0 5,487,988 - -0.7 5,487,988 - +0.2 5,487,988 - +1.4 5,487,988 - +0.3 5,487,988 - +1.9 5,487,988 - -0.2 5,487,988 - +1.4 5,487,988 - +1.2 5,487,993 + -0.1 5,487,994 - +0.4 5,488,006 - +0.5 5,488,017 + +1.0 5,488,018 - +0.2 5,488,018 - +0.4 5,488,030 + -0.0 5,488,057 + +0.2 5,488,057 + +1.1 5,488,057 + -0.6 5,488,057 + +0.6 5,488,058 - +0.3 5,488,058 - +0.1 5,488,058 - -0.2 5,488,058 - -0.7 5,488,058 - -0.5 5,488,141 + -1.1 5,488,142 - +1.4 5,488,150 + +0.6 5,488,150 + +1.6 5,488,150 + +0.6 5,488,150 + -0.0 5,488,150 + +0.0 5,488,150 + +0.0 5,488,150 + +0.4 5,488,150 + -0.0 5,488,151 - +0.6 5,488,151 - +1.1 5,488,175 + +0.6 5,488,175 + +0.4 5,488,175 + -1.0 5,488,176 - +0.6 5,488,427 + Pf6N2E2_4751 0.60 -0.2 5,488,427 + Pf6N2E2_4751 0.60 +1.6 5,488,428 - Pf6N2E2_4751 0.60 +1.3 5,488,428 - Pf6N2E2_4751 0.60 +0.2 5,488,477 + Pf6N2E2_4751 0.71 +0.2 5,488,605 + -0.0 5,488,605 + -0.2 5,488,695 + -0.3 5,488,695 + -0.1 5,488,696 - -0.2 5,488,772 + -0.6 5,488,772 + -1.0 5,488,772 + -1.8 5,488,772 + -0.3 5,488,772 + +0.6 5,488,772 + -0.3 5,488,773 - -0.2 5,488,773 - -0.0 5,488,894 + +0.2 5,488,895 - -2.4 5,488,984 + -2.3 5,488,985 - -2.4 5,488,985 - -2.8 5,489,105 + Pf6N2E2_4752 0.12 -4.1 5,489,105 + Pf6N2E2_4752 0.12 -0.7 5,489,105 + Pf6N2E2_4752 0.12 -1.4 5,489,105 + Pf6N2E2_4752 0.12 -3.9 5,489,105 + Pf6N2E2_4752 0.12 -2.1 5,489,106 - Pf6N2E2_4752 0.12 -1.9 5,489,197 + Pf6N2E2_4752 0.17 -3.1 5,489,197 + Pf6N2E2_4752 0.17 -1.1 5,489,197 + Pf6N2E2_4752 0.17 -1.6 5,489,197 + Pf6N2E2_4752 0.17 -3.5 5,489,356 + Pf6N2E2_4752 0.24 -2.6 5,489,545 + Pf6N2E2_4752 0.33 -1.8 5,489,546 - Pf6N2E2_4752 0.33 -4.2 5,489,546 - Pf6N2E2_4752 0.33 -2.6 5,489,744 + Pf6N2E2_4752 0.42 -0.8 5,489,763 + Pf6N2E2_4752 0.43 -3.1 5,489,763 + Pf6N2E2_4752 0.43 -3.7 5,489,763 + Pf6N2E2_4752 0.43 +0.2 5,489,764 - Pf6N2E2_4752 0.43 -2.9 5,489,764 - Pf6N2E2_4752 0.43 -1.3 5,489,764 - Pf6N2E2_4752 0.43 -1.8 5,489,764 - Pf6N2E2_4752 0.43 -2.2 5,489,764 - Pf6N2E2_4752 0.43 -2.9 5,489,764 - Pf6N2E2_4752 0.43 -3.6 5,489,839 + Pf6N2E2_4752 0.46 -3.0 5,489,839 + Pf6N2E2_4752 0.46 -2.0 5,489,839 + Pf6N2E2_4752 0.46 -3.3 5,489,839 + Pf6N2E2_4752 0.46 -2.4 5,489,839 + Pf6N2E2_4752 0.46 -2.0 5,489,839 + Pf6N2E2_4752 0.46 -3.4 5,489,840 - Pf6N2E2_4752 0.46 -1.3 5,489,927 + Pf6N2E2_4752 0.50 -3.4 5,489,927 + Pf6N2E2_4752 0.50 -3.0 5,489,928 - Pf6N2E2_4752 0.50 -2.1 5,489,928 - Pf6N2E2_4752 0.50 -0.6 5,489,928 - Pf6N2E2_4752 0.50 -1.3 5,489,928 - Pf6N2E2_4752 0.50 -2.3 5,490,052 + Pf6N2E2_4752 0.56 -3.5 5,490,052 + Pf6N2E2_4752 0.56 -2.5 5,490,149 - Pf6N2E2_4752 0.60 -2.9 5,490,149 - Pf6N2E2_4752 0.60 -1.4 5,490,436 + Pf6N2E2_4752 0.73 -3.2 5,490,437 - Pf6N2E2_4752 0.74 +0.2 5,490,586 + Pf6N2E2_4752 0.80 -1.5 5,490,587 - Pf6N2E2_4752 0.80 -2.8 5,490,587 - Pf6N2E2_4752 0.80 -1.8 5,490,587 - Pf6N2E2_4752 0.80 -1.9 5,490,598 + Pf6N2E2_4752 0.81 -2.4 5,490,599 - Pf6N2E2_4752 0.81 -1.9 5,490,599 - Pf6N2E2_4752 0.81 -2.9 5,490,713 + Pf6N2E2_4752 0.86 -2.7 5,490,714 - Pf6N2E2_4752 0.86 -1.4 5,490,745 + Pf6N2E2_4752 0.88 -2.8 5,490,746 - Pf6N2E2_4752 0.88 -2.9 5,490,746 - Pf6N2E2_4752 0.88 -2.3 5,490,772 + Pf6N2E2_4752 0.89 +0.5 5,490,772 + Pf6N2E2_4752 0.89 -1.6 5,490,772 + Pf6N2E2_4752 0.89 -1.0 5,490,773 - Pf6N2E2_4752 0.89 -2.4 5,490,856 - -2.4 5,491,036 + -3.8 5,491,036 + -1.8 5,491,036 + -1.3 5,491,037 - +0.6 5,491,085 + -2.5 5,491,085 + -2.8 5,491,085 + -0.0 5,491,086 - +0.2 5,491,086 - -0.8 5,491,086 - +0.4 5,491,086 - -2.2 5,491,086 - +0.6 5,491,086 - +0.8 5,491,109 - -0.6 5,491,109 - -1.2 5,491,109 - -1.1 5,491,109 - -0.1 5,491,135 + -1.3 5,491,135 + -0.4 5,491,135 + +0.7 5,491,136 - -1.0 5,491,178 - +0.2 5,491,191 + +0.0 5,491,191 + +0.4 5,491,191 + -0.2 5,491,191 + -0.2 5,491,192 - +0.4 5,491,192 - -0.3 5,491,192 - -1.1 5,491,192 - +0.0 5,491,199 + +0.1 5,491,199 + -0.3 5,491,199 + +0.8 5,491,199 + +0.1 5,491,199 + -0.2 5,491,200 - +0.9 5,491,200 - -0.2 5,491,200 - +0.1 5,491,200 - +0.1 5,491,200 - -0.3 5,491,200 - -0.0 5,491,200 - -0.7 5,491,200 - +0.2 5,491,200 - +0.1 5,491,200 - -0.4 5,491,200 - +0.1 5,491,203 + -0.1 5,491,203 + +1.1 5,491,203 + +0.6 5,491,204 - +0.1 5,491,204 - +0.4 5,491,220 + -0.2 5,491,221 - -0.3 5,491,221 - -0.3 5,491,221 - -0.7 5,491,221 - -0.8 5,491,223 + +0.6 5,491,223 + +0.5 5,491,223 + -0.6 5,491,223 + +0.1 5,491,223 + -1.1 5,491,223 + -0.2 5,491,223 + -0.5 5,491,223 + +0.3 5,491,223 + +0.1 5,491,223 + -0.4 5,491,224 - +0.5 5,491,224 - -0.4 5,491,224 - +0.7 5,491,224 - +1.5 5,491,224 - +0.8 5,491,224 - +0.1 5,491,224 - +0.6 5,491,224 - +0.4 5,491,224 - -1.5 5,491,224 - +0.6 5,491,224 - +1.2 5,491,255 + +1.0 5,491,255 + +0.6 5,491,255 + +0.6 5,491,255 + -0.9 5,491,255 + +0.3 5,491,255 + +0.9 5,491,256 - +0.1 5,491,256 - -0.4 5,491,280 - -1.0 5,491,287 + +2.9 5,491,288 - +0.9 5,491,423 + +0.7 5,491,492 + +0.3 5,491,492 + +1.0 5,491,492 + +0.8 5,491,492 + -0.4 5,491,492 + +1.1 5,491,492 + -0.3 5,491,492 + -0.3 5,491,493 - -0.3 5,491,493 - -0.6 5,491,493 - +0.8 5,491,493 - -0.0 5,491,493 - +0.3 5,491,493 - +0.8 5,491,493 - -0.3 5,491,493 - +1.3 5,491,494 + +0.1 5,491,495 - -0.3 5,491,495 - -0.3 5,491,495 - -0.1 5,491,495 - +0.3 5,491,495 - +0.8 5,491,495 - +1.5 5,491,495 - +0.7 5,491,495 - -0.4 5,491,730 + Pf6N2E2_4753 0.39 +0.1 5,491,730 + Pf6N2E2_4753 0.39 -1.7 5,491,731 - Pf6N2E2_4753 0.39 +1.6 5,491,731 - Pf6N2E2_4753 0.39 -0.5 5,491,731 - Pf6N2E2_4753 0.39 -0.3 5,491,731 - Pf6N2E2_4753 0.39 +0.5 5,491,827 + Pf6N2E2_4753 0.58 -0.9 5,491,828 - Pf6N2E2_4753 0.58 +1.1 5,491,889 + Pf6N2E2_4753 0.70 +0.8 5,491,889 + Pf6N2E2_4753 0.70 +0.6 5,491,889 + Pf6N2E2_4753 0.70 -0.4 5,491,889 + Pf6N2E2_4753 0.70 +0.6 5,491,889 + Pf6N2E2_4753 0.70 +0.1 5,491,889 + Pf6N2E2_4753 0.70 +0.9 5,491,890 - Pf6N2E2_4753 0.70 -0.2 5,491,890 - Pf6N2E2_4753 0.70 +1.3 5,491,890 - Pf6N2E2_4753 0.70 -0.5 5,491,890 - Pf6N2E2_4753 0.70 +0.4 5,491,890 - Pf6N2E2_4753 0.70 +1.1
Or see this region's nucleotide sequence