Experiment: D-Glucosamine Hydrochloride (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4673 and Pf6N2E2_4674 are separated by 265 nucleotides Pf6N2E2_4674 and Pf6N2E2_4675 are separated by 194 nucleotides
Pf6N2E2_4673 - ATP-dependent 23S rRNA helicase DbpA, at 5,403,949 to 5,405,334
_4673
Pf6N2E2_4674 - Permeases of the major facilitator superfamily, at 5,405,600 to 5,407,027
_4674
Pf6N2E2_4675 - TldE/PmbA family protein, Beta/Gamma-proteobacterial subgroup, at 5,407,222 to 5,408,559
_4675
Position (kb)
5405
5406
5407
5408 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 5404.657 kb on + strand, within Pf6N2E2_4673 at 5404.657 kb on + strand, within Pf6N2E2_4673 at 5404.658 kb on - strand, within Pf6N2E2_4673 at 5404.664 kb on + strand, within Pf6N2E2_4673 at 5404.665 kb on - strand, within Pf6N2E2_4673 at 5404.767 kb on - strand, within Pf6N2E2_4673 at 5405.310 kb on + strand at 5405.310 kb on + strand at 5405.369 kb on + strand at 5405.369 kb on + strand at 5405.369 kb on + strand at 5405.370 kb on - strand at 5405.370 kb on - strand at 5405.466 kb on - strand at 5405.492 kb on - strand at 5405.602 kb on - strand at 5405.602 kb on - strand at 5405.602 kb on - strand at 5405.602 kb on - strand at 5405.602 kb on - strand at 5405.611 kb on + strand at 5405.612 kb on - strand at 5406.132 kb on + strand, within Pf6N2E2_4674 at 5406.291 kb on + strand, within Pf6N2E2_4674 at 5406.291 kb on + strand, within Pf6N2E2_4674 at 5406.480 kb on + strand, within Pf6N2E2_4674 at 5406.481 kb on - strand, within Pf6N2E2_4674 at 5406.538 kb on - strand, within Pf6N2E2_4674 at 5406.855 kb on + strand, within Pf6N2E2_4674 at 5406.855 kb on + strand, within Pf6N2E2_4674 at 5406.855 kb on + strand, within Pf6N2E2_4674 at 5406.856 kb on - strand, within Pf6N2E2_4674 at 5406.856 kb on - strand, within Pf6N2E2_4674 at 5407.039 kb on + strand at 5407.039 kb on + strand at 5407.039 kb on + strand at 5407.039 kb on + strand at 5407.039 kb on + strand at 5407.040 kb on - strand at 5407.040 kb on - strand at 5407.040 kb on - strand at 5407.040 kb on - strand at 5407.040 kb on - strand at 5407.040 kb on - strand at 5407.040 kb on - strand at 5407.064 kb on - strand at 5407.208 kb on + strand at 5407.223 kb on + strand at 5407.223 kb on + strand at 5407.223 kb on + strand at 5407.223 kb on + strand at 5407.224 kb on - strand at 5407.224 kb on - strand at 5407.224 kb on - strand at 5407.224 kb on - strand at 5407.224 kb on - strand at 5407.292 kb on + strand at 5407.292 kb on + strand at 5407.293 kb on - strand at 5407.293 kb on - strand at 5407.361 kb on + strand, within Pf6N2E2_4675 at 5407.476 kb on - strand, within Pf6N2E2_4675 at 5407.502 kb on + strand, within Pf6N2E2_4675 at 5407.503 kb on - strand, within Pf6N2E2_4675 at 5407.503 kb on - strand, within Pf6N2E2_4675 at 5407.503 kb on - strand, within Pf6N2E2_4675 at 5407.503 kb on - strand, within Pf6N2E2_4675 at 5407.532 kb on + strand, within Pf6N2E2_4675 at 5407.532 kb on + strand, within Pf6N2E2_4675 at 5407.532 kb on + strand, within Pf6N2E2_4675 at 5407.532 kb on + strand, within Pf6N2E2_4675 at 5407.532 kb on + strand, within Pf6N2E2_4675 at 5407.533 kb on - strand, within Pf6N2E2_4675 at 5407.533 kb on - strand, within Pf6N2E2_4675 at 5407.533 kb on - strand, within Pf6N2E2_4675 at 5407.533 kb on - strand, within Pf6N2E2_4675 at 5407.533 kb on - strand, within Pf6N2E2_4675 at 5407.533 kb on - strand, within Pf6N2E2_4675 at 5407.533 kb on - strand, within Pf6N2E2_4675 at 5407.533 kb on - strand, within Pf6N2E2_4675 at 5407.533 kb on - strand, within Pf6N2E2_4675 at 5407.533 kb on - strand, within Pf6N2E2_4675 at 5407.533 kb on - strand, within Pf6N2E2_4675 at 5407.583 kb on + strand, within Pf6N2E2_4675 at 5407.583 kb on + strand, within Pf6N2E2_4675 at 5407.583 kb on + strand, within Pf6N2E2_4675 at 5407.584 kb on - strand, within Pf6N2E2_4675 at 5407.584 kb on - strand, within Pf6N2E2_4675 at 5407.584 kb on - strand, within Pf6N2E2_4675 at 5407.880 kb on + strand, within Pf6N2E2_4675 at 5407.880 kb on + strand, within Pf6N2E2_4675 at 5407.881 kb on - strand, within Pf6N2E2_4675 at 5407.998 kb on - strand, within Pf6N2E2_4675 at 5407.998 kb on - strand, within Pf6N2E2_4675
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (C) remove 5,404,657 + Pf6N2E2_4673 0.51 -0.1 5,404,657 + Pf6N2E2_4673 0.51 +0.9 5,404,658 - Pf6N2E2_4673 0.51 -0.9 5,404,664 + Pf6N2E2_4673 0.52 +0.3 5,404,665 - Pf6N2E2_4673 0.52 -1.2 5,404,767 - Pf6N2E2_4673 0.59 +0.8 5,405,310 + -0.2 5,405,310 + +2.8 5,405,369 + +0.8 5,405,369 + -2.3 5,405,369 + -0.6 5,405,370 - -0.0 5,405,370 - +1.6 5,405,466 - +0.2 5,405,492 - -0.0 5,405,602 - +0.1 5,405,602 - +0.1 5,405,602 - +0.3 5,405,602 - +1.3 5,405,602 - -2.4 5,405,611 + +0.7 5,405,612 - +0.1 5,406,132 + Pf6N2E2_4674 0.37 -3.2 5,406,291 + Pf6N2E2_4674 0.48 -0.8 5,406,291 + Pf6N2E2_4674 0.48 -0.1 5,406,480 + Pf6N2E2_4674 0.62 -1.2 5,406,481 - Pf6N2E2_4674 0.62 +0.2 5,406,538 - Pf6N2E2_4674 0.66 -1.4 5,406,855 + Pf6N2E2_4674 0.88 -0.5 5,406,855 + Pf6N2E2_4674 0.88 +0.3 5,406,855 + Pf6N2E2_4674 0.88 -0.3 5,406,856 - Pf6N2E2_4674 0.88 -1.8 5,406,856 - Pf6N2E2_4674 0.88 -0.0 5,407,039 + +0.1 5,407,039 + -0.2 5,407,039 + -0.3 5,407,039 + +0.4 5,407,039 + +0.3 5,407,040 - +0.7 5,407,040 - +0.1 5,407,040 - +0.7 5,407,040 - +0.3 5,407,040 - -1.2 5,407,040 - -0.4 5,407,040 - -2.5 5,407,064 - +0.3 5,407,208 + -0.9 5,407,223 + +0.3 5,407,223 + +0.6 5,407,223 + +0.4 5,407,223 + +0.5 5,407,224 - -0.6 5,407,224 - +0.5 5,407,224 - +0.2 5,407,224 - +0.6 5,407,224 - +0.6 5,407,292 + -0.8 5,407,292 + +0.6 5,407,293 - -1.2 5,407,293 - +0.0 5,407,361 + Pf6N2E2_4675 0.10 +1.0 5,407,476 - Pf6N2E2_4675 0.19 -0.7 5,407,502 + Pf6N2E2_4675 0.21 -0.9 5,407,503 - Pf6N2E2_4675 0.21 +0.5 5,407,503 - Pf6N2E2_4675 0.21 +0.5 5,407,503 - Pf6N2E2_4675 0.21 +0.1 5,407,503 - Pf6N2E2_4675 0.21 -1.7 5,407,532 + Pf6N2E2_4675 0.23 -0.8 5,407,532 + Pf6N2E2_4675 0.23 +1.2 5,407,532 + Pf6N2E2_4675 0.23 +0.4 5,407,532 + Pf6N2E2_4675 0.23 +1.4 5,407,532 + Pf6N2E2_4675 0.23 +0.3 5,407,533 - Pf6N2E2_4675 0.23 +0.6 5,407,533 - Pf6N2E2_4675 0.23 +0.2 5,407,533 - Pf6N2E2_4675 0.23 +0.2 5,407,533 - Pf6N2E2_4675 0.23 +0.9 5,407,533 - Pf6N2E2_4675 0.23 -0.2 5,407,533 - Pf6N2E2_4675 0.23 +1.3 5,407,533 - Pf6N2E2_4675 0.23 -1.5 5,407,533 - Pf6N2E2_4675 0.23 -0.5 5,407,533 - Pf6N2E2_4675 0.23 -0.3 5,407,533 - Pf6N2E2_4675 0.23 +0.1 5,407,533 - Pf6N2E2_4675 0.23 +0.7 5,407,583 + Pf6N2E2_4675 0.27 -0.6 5,407,583 + Pf6N2E2_4675 0.27 +0.1 5,407,583 + Pf6N2E2_4675 0.27 +0.2 5,407,584 - Pf6N2E2_4675 0.27 -0.7 5,407,584 - Pf6N2E2_4675 0.27 +0.4 5,407,584 - Pf6N2E2_4675 0.27 +0.6 5,407,880 + Pf6N2E2_4675 0.49 -0.9 5,407,880 + Pf6N2E2_4675 0.49 -0.3 5,407,881 - Pf6N2E2_4675 0.49 +1.2 5,407,998 - Pf6N2E2_4675 0.58 +0.6 5,407,998 - Pf6N2E2_4675 0.58 +0.1
Or see this region's nucleotide sequence