Experiment: D-Glucosamine Hydrochloride (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4622 and Pf6N2E2_4623 overlap by 4 nucleotides Pf6N2E2_4623 and Pf6N2E2_4624 overlap by 4 nucleotides Pf6N2E2_4624 and Pf6N2E2_4625 overlap by 4 nucleotides Pf6N2E2_4625 and Pf6N2E2_4626 are separated by 67 nucleotides
Pf6N2E2_4622 - Malonate decarboxylase beta subunit, at 5,351,960 to 5,352,811
_4622
Pf6N2E2_4623 - Malonate decarboxylase gamma subunit, at 5,352,808 to 5,353,611
_4623
Pf6N2E2_4624 - Phosphoribosyl-dephospho-CoA transferase (EC 2.7.7.-), at 5,353,608 to 5,354,228
_4624
Pf6N2E2_4625 - Malonyl CoA acyl carrier protein transacylase (EC 2.3.1.39), at 5,354,225 to 5,355,145
_4625
Pf6N2E2_4626 - Malonate transporter, MadL subunit, at 5,355,213 to 5,355,629
_4626
Position (kb) 5353
5354
5355 Strain fitness (log2 ratio) -1
0
1
2 at 5352.629 kb on + strand at 5352.629 kb on + strand, within Pf6N2E2_4622 at 5352.630 kb on - strand, within Pf6N2E2_4622 at 5352.630 kb on - strand, within Pf6N2E2_4622 at 5352.630 kb on - strand, within Pf6N2E2_4622 at 5352.630 kb on - strand, within Pf6N2E2_4622 at 5352.791 kb on + strand at 5352.792 kb on - strand at 5352.817 kb on + strand at 5352.818 kb on - strand at 5353.108 kb on + strand, within Pf6N2E2_4623 at 5353.109 kb on - strand, within Pf6N2E2_4623 at 5353.109 kb on - strand, within Pf6N2E2_4623 at 5353.168 kb on + strand, within Pf6N2E2_4623 at 5353.169 kb on - strand, within Pf6N2E2_4623 at 5353.169 kb on - strand, within Pf6N2E2_4623 at 5353.169 kb on - strand, within Pf6N2E2_4623 at 5353.253 kb on - strand, within Pf6N2E2_4623 at 5353.390 kb on + strand, within Pf6N2E2_4623 at 5353.390 kb on + strand, within Pf6N2E2_4623 at 5353.390 kb on + strand, within Pf6N2E2_4623 at 5353.391 kb on - strand, within Pf6N2E2_4623 at 5353.391 kb on - strand, within Pf6N2E2_4623 at 5353.391 kb on - strand, within Pf6N2E2_4623 at 5353.391 kb on - strand, within Pf6N2E2_4623 at 5353.391 kb on - strand, within Pf6N2E2_4623 at 5353.558 kb on + strand at 5353.559 kb on - strand at 5353.613 kb on + strand at 5353.613 kb on + strand at 5353.613 kb on + strand at 5353.613 kb on + strand at 5353.613 kb on + strand at 5353.613 kb on + strand at 5353.614 kb on - strand at 5353.614 kb on - strand at 5353.614 kb on - strand at 5353.614 kb on - strand at 5353.614 kb on - strand at 5353.614 kb on - strand at 5353.617 kb on + strand at 5353.617 kb on + strand at 5353.617 kb on + strand at 5353.617 kb on + strand at 5353.617 kb on + strand at 5353.617 kb on + strand at 5353.617 kb on + strand at 5353.617 kb on + strand at 5353.617 kb on + strand at 5353.617 kb on + strand at 5353.617 kb on + strand at 5353.618 kb on - strand at 5353.618 kb on - strand at 5353.618 kb on - strand at 5353.618 kb on - strand at 5353.618 kb on - strand at 5353.618 kb on - strand at 5353.618 kb on - strand at 5353.618 kb on - strand at 5353.618 kb on - strand at 5353.618 kb on - strand at 5353.789 kb on - strand, within Pf6N2E2_4624 at 5354.450 kb on + strand, within Pf6N2E2_4625 at 5354.451 kb on - strand, within Pf6N2E2_4625 at 5354.451 kb on - strand, within Pf6N2E2_4625 at 5354.451 kb on - strand, within Pf6N2E2_4625 at 5354.564 kb on + strand, within Pf6N2E2_4625 at 5354.873 kb on + strand, within Pf6N2E2_4625 at 5354.985 kb on - strand, within Pf6N2E2_4625 at 5355.143 kb on + strand at 5355.143 kb on + strand at 5355.206 kb on + strand at 5355.206 kb on + strand at 5355.207 kb on - strand at 5355.218 kb on + strand at 5355.218 kb on + strand at 5355.218 kb on + strand at 5355.218 kb on + strand at 5355.218 kb on + strand at 5355.219 kb on - strand at 5355.222 kb on + strand at 5355.222 kb on + strand at 5355.222 kb on + strand at 5355.222 kb on + strand at 5355.223 kb on - strand at 5355.223 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (C) remove 5,352,629 + +0.9 5,352,629 + Pf6N2E2_4622 0.79 +0.4 5,352,630 - Pf6N2E2_4622 0.79 +0.2 5,352,630 - Pf6N2E2_4622 0.79 -0.3 5,352,630 - Pf6N2E2_4622 0.79 +0.2 5,352,630 - Pf6N2E2_4622 0.79 +0.2 5,352,791 + -0.3 5,352,792 - +0.0 5,352,817 + +0.5 5,352,818 - +1.0 5,353,108 + Pf6N2E2_4623 0.37 +0.6 5,353,109 - Pf6N2E2_4623 0.37 -0.3 5,353,109 - Pf6N2E2_4623 0.37 -0.2 5,353,168 + Pf6N2E2_4623 0.45 +0.3 5,353,169 - Pf6N2E2_4623 0.45 +0.3 5,353,169 - Pf6N2E2_4623 0.45 +0.3 5,353,169 - Pf6N2E2_4623 0.45 -0.1 5,353,253 - Pf6N2E2_4623 0.55 +0.2 5,353,390 + Pf6N2E2_4623 0.72 -1.1 5,353,390 + Pf6N2E2_4623 0.72 +0.9 5,353,390 + Pf6N2E2_4623 0.72 +0.1 5,353,391 - Pf6N2E2_4623 0.73 -0.7 5,353,391 - Pf6N2E2_4623 0.73 +1.0 5,353,391 - Pf6N2E2_4623 0.73 +0.5 5,353,391 - Pf6N2E2_4623 0.73 +0.0 5,353,391 - Pf6N2E2_4623 0.73 +0.1 5,353,558 + -0.3 5,353,559 - -0.5 5,353,613 + -0.3 5,353,613 + +0.4 5,353,613 + +0.2 5,353,613 + +0.3 5,353,613 + +0.4 5,353,613 + +0.9 5,353,614 - -0.5 5,353,614 - -0.4 5,353,614 - +1.5 5,353,614 - +0.0 5,353,614 - +0.7 5,353,614 - -0.5 5,353,617 + +0.9 5,353,617 + +0.1 5,353,617 + -0.5 5,353,617 + +1.4 5,353,617 + +0.1 5,353,617 + +1.0 5,353,617 + -1.4 5,353,617 + +0.2 5,353,617 + -0.3 5,353,617 + +0.2 5,353,617 + +0.4 5,353,618 - +0.2 5,353,618 - +0.0 5,353,618 - +0.2 5,353,618 - +1.0 5,353,618 - +0.4 5,353,618 - +1.4 5,353,618 - +0.7 5,353,618 - +0.8 5,353,618 - -0.3 5,353,618 - +0.6 5,353,789 - Pf6N2E2_4624 0.29 -0.6 5,354,450 + Pf6N2E2_4625 0.24 -0.1 5,354,451 - Pf6N2E2_4625 0.25 +0.3 5,354,451 - Pf6N2E2_4625 0.25 -0.0 5,354,451 - Pf6N2E2_4625 0.25 +0.4 5,354,564 + Pf6N2E2_4625 0.37 -1.3 5,354,873 + Pf6N2E2_4625 0.70 +0.1 5,354,985 - Pf6N2E2_4625 0.83 +0.4 5,355,143 + +0.5 5,355,143 + +1.6 5,355,206 + +0.3 5,355,206 + +0.4 5,355,207 - +1.9 5,355,218 + +0.4 5,355,218 + +1.0 5,355,218 + -1.2 5,355,218 + -0.3 5,355,218 + +0.1 5,355,219 - -0.4 5,355,222 + +0.5 5,355,222 + +0.2 5,355,222 + +1.1 5,355,222 + +0.5 5,355,223 - +0.7 5,355,223 - +0.8
Or see this region's nucleotide sequence