Experiment: D-Glucosamine Hydrochloride (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4452 and Pf6N2E2_4453 are separated by 17 nucleotides Pf6N2E2_4453 and Pf6N2E2_4454 are separated by 147 nucleotides
Pf6N2E2_4452 - Porphobilinogen synthase (EC 4.2.1.24), at 5,183,026 to 5,184,039
_4452
Pf6N2E2_4453 - Polyphosphate kinase (EC 2.7.4.1), at 5,184,057 to 5,186,273
_4453
Pf6N2E2_4454 - Exopolyphosphatase (EC 3.6.1.11), at 5,186,421 to 5,187,923
_4454
Position (kb) 5184
5185
5186
5187 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5183.108 kb on + strand at 5183.108 kb on + strand at 5183.109 kb on - strand at 5183.109 kb on - strand at 5183.446 kb on - strand, within Pf6N2E2_4452 at 5183.465 kb on - strand, within Pf6N2E2_4452 at 5183.465 kb on - strand, within Pf6N2E2_4452 at 5183.630 kb on - strand, within Pf6N2E2_4452 at 5183.641 kb on + strand, within Pf6N2E2_4452 at 5183.642 kb on - strand, within Pf6N2E2_4452 at 5183.642 kb on - strand, within Pf6N2E2_4452 at 5183.653 kb on + strand, within Pf6N2E2_4452 at 5183.654 kb on - strand, within Pf6N2E2_4452 at 5183.654 kb on - strand, within Pf6N2E2_4452 at 5183.656 kb on + strand, within Pf6N2E2_4452 at 5183.656 kb on + strand, within Pf6N2E2_4452 at 5183.656 kb on + strand, within Pf6N2E2_4452 at 5183.657 kb on - strand, within Pf6N2E2_4452 at 5183.657 kb on - strand, within Pf6N2E2_4452 at 5183.720 kb on - strand, within Pf6N2E2_4452 at 5183.720 kb on - strand, within Pf6N2E2_4452 at 5183.720 kb on - strand, within Pf6N2E2_4452 at 5183.818 kb on + strand, within Pf6N2E2_4452 at 5183.818 kb on + strand, within Pf6N2E2_4452 at 5183.890 kb on + strand, within Pf6N2E2_4452 at 5183.996 kb on - strand at 5183.996 kb on - strand at 5183.996 kb on - strand at 5183.996 kb on - strand at 5183.996 kb on - strand at 5184.023 kb on + strand at 5184.024 kb on - strand at 5184.037 kb on + strand at 5184.037 kb on + strand at 5184.038 kb on - strand at 5184.088 kb on + strand at 5184.219 kb on + strand at 5184.219 kb on + strand at 5184.219 kb on + strand at 5184.219 kb on + strand at 5184.219 kb on + strand at 5184.219 kb on + strand at 5184.219 kb on + strand at 5184.219 kb on + strand at 5184.219 kb on + strand at 5184.220 kb on - strand at 5184.220 kb on - strand at 5184.220 kb on - strand at 5184.220 kb on - strand at 5184.481 kb on - strand, within Pf6N2E2_4453 at 5184.481 kb on - strand, within Pf6N2E2_4453 at 5184.481 kb on - strand, within Pf6N2E2_4453 at 5184.585 kb on + strand, within Pf6N2E2_4453 at 5184.801 kb on + strand, within Pf6N2E2_4453 at 5184.801 kb on + strand, within Pf6N2E2_4453 at 5184.801 kb on + strand, within Pf6N2E2_4453 at 5184.801 kb on + strand, within Pf6N2E2_4453 at 5184.801 kb on + strand, within Pf6N2E2_4453 at 5184.801 kb on + strand, within Pf6N2E2_4453 at 5184.801 kb on + strand, within Pf6N2E2_4453 at 5184.801 kb on + strand, within Pf6N2E2_4453 at 5184.801 kb on + strand, within Pf6N2E2_4453 at 5184.801 kb on + strand, within Pf6N2E2_4453 at 5184.801 kb on + strand, within Pf6N2E2_4453 at 5184.801 kb on + strand, within Pf6N2E2_4453 at 5184.801 kb on + strand, within Pf6N2E2_4453 at 5184.802 kb on - strand, within Pf6N2E2_4453 at 5184.802 kb on - strand, within Pf6N2E2_4453 at 5184.802 kb on - strand, within Pf6N2E2_4453 at 5184.802 kb on - strand, within Pf6N2E2_4453 at 5184.802 kb on - strand, within Pf6N2E2_4453 at 5184.802 kb on - strand, within Pf6N2E2_4453 at 5184.802 kb on - strand, within Pf6N2E2_4453 at 5184.802 kb on - strand, within Pf6N2E2_4453 at 5184.802 kb on - strand, within Pf6N2E2_4453 at 5184.802 kb on - strand, within Pf6N2E2_4453 at 5184.802 kb on - strand, within Pf6N2E2_4453 at 5184.802 kb on - strand, within Pf6N2E2_4453 at 5184.802 kb on - strand, within Pf6N2E2_4453 at 5184.802 kb on - strand, within Pf6N2E2_4453 at 5184.802 kb on - strand, within Pf6N2E2_4453 at 5184.802 kb on - strand, within Pf6N2E2_4453 at 5184.802 kb on - strand, within Pf6N2E2_4453 at 5184.802 kb on - strand, within Pf6N2E2_4453 at 5184.802 kb on - strand, within Pf6N2E2_4453 at 5184.802 kb on - strand, within Pf6N2E2_4453 at 5184.802 kb on - strand, within Pf6N2E2_4453 at 5184.802 kb on - strand, within Pf6N2E2_4453 at 5184.802 kb on - strand, within Pf6N2E2_4453 at 5184.802 kb on - strand, within Pf6N2E2_4453 at 5184.802 kb on - strand, within Pf6N2E2_4453 at 5184.873 kb on + strand, within Pf6N2E2_4453 at 5184.873 kb on + strand, within Pf6N2E2_4453 at 5184.874 kb on - strand, within Pf6N2E2_4453 at 5185.029 kb on + strand, within Pf6N2E2_4453 at 5185.030 kb on - strand, within Pf6N2E2_4453 at 5185.068 kb on + strand, within Pf6N2E2_4453 at 5185.068 kb on + strand, within Pf6N2E2_4453 at 5185.069 kb on - strand, within Pf6N2E2_4453 at 5185.144 kb on - strand, within Pf6N2E2_4453 at 5185.321 kb on - strand, within Pf6N2E2_4453 at 5185.479 kb on + strand, within Pf6N2E2_4453 at 5185.479 kb on + strand, within Pf6N2E2_4453 at 5185.479 kb on + strand, within Pf6N2E2_4453 at 5185.479 kb on + strand, within Pf6N2E2_4453 at 5185.480 kb on - strand, within Pf6N2E2_4453 at 5185.480 kb on - strand, within Pf6N2E2_4453 at 5185.480 kb on - strand, within Pf6N2E2_4453 at 5185.480 kb on - strand, within Pf6N2E2_4453 at 5185.480 kb on - strand, within Pf6N2E2_4453 at 5185.487 kb on - strand, within Pf6N2E2_4453 at 5185.578 kb on + strand, within Pf6N2E2_4453 at 5185.578 kb on + strand, within Pf6N2E2_4453 at 5185.578 kb on + strand, within Pf6N2E2_4453 at 5185.579 kb on - strand, within Pf6N2E2_4453 at 5185.579 kb on - strand, within Pf6N2E2_4453 at 5185.579 kb on - strand, within Pf6N2E2_4453 at 5185.602 kb on + strand, within Pf6N2E2_4453 at 5185.603 kb on - strand, within Pf6N2E2_4453 at 5185.611 kb on + strand, within Pf6N2E2_4453 at 5185.611 kb on + strand, within Pf6N2E2_4453 at 5185.611 kb on + strand, within Pf6N2E2_4453 at 5185.612 kb on - strand, within Pf6N2E2_4453 at 5185.612 kb on - strand, within Pf6N2E2_4453 at 5185.612 kb on - strand, within Pf6N2E2_4453 at 5185.796 kb on + strand, within Pf6N2E2_4453 at 5185.848 kb on + strand, within Pf6N2E2_4453 at 5185.848 kb on + strand, within Pf6N2E2_4453 at 5185.849 kb on - strand, within Pf6N2E2_4453 at 5185.849 kb on - strand, within Pf6N2E2_4453 at 5185.849 kb on - strand, within Pf6N2E2_4453 at 5185.945 kb on + strand, within Pf6N2E2_4453 at 5185.945 kb on + strand, within Pf6N2E2_4453 at 5185.945 kb on + strand, within Pf6N2E2_4453 at 5185.945 kb on + strand, within Pf6N2E2_4453 at 5185.945 kb on + strand, within Pf6N2E2_4453 at 5185.945 kb on + strand, within Pf6N2E2_4453 at 5185.945 kb on + strand, within Pf6N2E2_4453 at 5185.945 kb on + strand, within Pf6N2E2_4453 at 5185.945 kb on + strand, within Pf6N2E2_4453 at 5185.946 kb on - strand, within Pf6N2E2_4453 at 5185.946 kb on - strand, within Pf6N2E2_4453 at 5185.946 kb on - strand, within Pf6N2E2_4453 at 5185.946 kb on - strand, within Pf6N2E2_4453 at 5185.946 kb on - strand, within Pf6N2E2_4453 at 5185.946 kb on - strand, within Pf6N2E2_4453 at 5185.946 kb on - strand, within Pf6N2E2_4453 at 5185.946 kb on - strand, within Pf6N2E2_4453 at 5185.946 kb on - strand, within Pf6N2E2_4453 at 5185.976 kb on + strand, within Pf6N2E2_4453 at 5185.976 kb on + strand, within Pf6N2E2_4453 at 5185.977 kb on - strand, within Pf6N2E2_4453 at 5185.977 kb on - strand, within Pf6N2E2_4453 at 5185.989 kb on + strand, within Pf6N2E2_4453 at 5185.989 kb on + strand, within Pf6N2E2_4453 at 5185.989 kb on + strand, within Pf6N2E2_4453 at 5185.989 kb on + strand, within Pf6N2E2_4453 at 5185.990 kb on - strand, within Pf6N2E2_4453 at 5186.130 kb on + strand at 5186.130 kb on + strand at 5186.130 kb on + strand at 5186.130 kb on + strand at 5186.130 kb on + strand at 5186.130 kb on + strand at 5186.178 kb on + strand at 5186.178 kb on + strand at 5186.178 kb on + strand at 5186.178 kb on + strand at 5186.178 kb on + strand at 5186.178 kb on + strand at 5186.179 kb on - strand at 5186.401 kb on + strand at 5186.401 kb on + strand at 5186.401 kb on + strand at 5186.401 kb on + strand at 5186.401 kb on + strand at 5186.401 kb on + strand at 5186.401 kb on + strand at 5186.401 kb on + strand at 5186.401 kb on + strand at 5186.401 kb on + strand at 5186.401 kb on + strand at 5186.401 kb on + strand at 5186.402 kb on - strand at 5186.402 kb on - strand at 5186.402 kb on - strand at 5186.402 kb on - strand at 5186.402 kb on - strand at 5186.402 kb on - strand at 5186.670 kb on - strand, within Pf6N2E2_4454 at 5186.767 kb on + strand, within Pf6N2E2_4454 at 5186.768 kb on - strand, within Pf6N2E2_4454 at 5186.768 kb on - strand, within Pf6N2E2_4454 at 5186.786 kb on - strand, within Pf6N2E2_4454 at 5186.791 kb on + strand, within Pf6N2E2_4454 at 5187.010 kb on - strand, within Pf6N2E2_4454 at 5187.016 kb on + strand, within Pf6N2E2_4454 at 5187.017 kb on - strand, within Pf6N2E2_4454
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (C) remove 5,183,108 + +1.8 5,183,108 + -1.1 5,183,109 - -0.3 5,183,109 - +0.3 5,183,446 - Pf6N2E2_4452 0.41 -0.3 5,183,465 - Pf6N2E2_4452 0.43 +0.8 5,183,465 - Pf6N2E2_4452 0.43 +0.2 5,183,630 - Pf6N2E2_4452 0.60 +0.2 5,183,641 + Pf6N2E2_4452 0.61 +1.0 5,183,642 - Pf6N2E2_4452 0.61 +0.2 5,183,642 - Pf6N2E2_4452 0.61 +0.7 5,183,653 + Pf6N2E2_4452 0.62 +0.1 5,183,654 - Pf6N2E2_4452 0.62 -2.0 5,183,654 - Pf6N2E2_4452 0.62 -0.0 5,183,656 + Pf6N2E2_4452 0.62 +1.2 5,183,656 + Pf6N2E2_4452 0.62 +1.3 5,183,656 + Pf6N2E2_4452 0.62 +0.3 5,183,657 - Pf6N2E2_4452 0.62 +0.2 5,183,657 - Pf6N2E2_4452 0.62 -0.3 5,183,720 - Pf6N2E2_4452 0.68 +0.6 5,183,720 - Pf6N2E2_4452 0.68 +0.3 5,183,720 - Pf6N2E2_4452 0.68 +1.0 5,183,818 + Pf6N2E2_4452 0.78 +1.3 5,183,818 + Pf6N2E2_4452 0.78 +0.8 5,183,890 + Pf6N2E2_4452 0.85 +0.6 5,183,996 - -1.6 5,183,996 - +0.3 5,183,996 - -3.0 5,183,996 - -0.1 5,183,996 - +0.7 5,184,023 + +1.2 5,184,024 - -1.6 5,184,037 + -0.7 5,184,037 + +0.7 5,184,038 - -0.6 5,184,088 + -2.2 5,184,219 + -3.3 5,184,219 + -0.9 5,184,219 + -1.4 5,184,219 + +0.4 5,184,219 + -1.6 5,184,219 + -2.4 5,184,219 + -1.3 5,184,219 + -0.2 5,184,219 + -3.0 5,184,220 - -1.5 5,184,220 - -0.5 5,184,220 - +1.5 5,184,220 - +0.2 5,184,481 - Pf6N2E2_4453 0.19 -1.3 5,184,481 - Pf6N2E2_4453 0.19 -0.5 5,184,481 - Pf6N2E2_4453 0.19 -0.9 5,184,585 + Pf6N2E2_4453 0.24 -1.0 5,184,801 + Pf6N2E2_4453 0.34 -1.5 5,184,801 + Pf6N2E2_4453 0.34 -0.8 5,184,801 + Pf6N2E2_4453 0.34 -0.3 5,184,801 + Pf6N2E2_4453 0.34 -1.5 5,184,801 + Pf6N2E2_4453 0.34 -1.8 5,184,801 + Pf6N2E2_4453 0.34 -2.6 5,184,801 + Pf6N2E2_4453 0.34 +0.0 5,184,801 + Pf6N2E2_4453 0.34 -0.9 5,184,801 + Pf6N2E2_4453 0.34 +0.2 5,184,801 + Pf6N2E2_4453 0.34 -0.3 5,184,801 + Pf6N2E2_4453 0.34 -2.5 5,184,801 + Pf6N2E2_4453 0.34 -1.2 5,184,801 + Pf6N2E2_4453 0.34 -0.1 5,184,802 - Pf6N2E2_4453 0.34 -0.1 5,184,802 - Pf6N2E2_4453 0.34 -0.3 5,184,802 - Pf6N2E2_4453 0.34 -2.1 5,184,802 - Pf6N2E2_4453 0.34 +0.5 5,184,802 - Pf6N2E2_4453 0.34 -2.2 5,184,802 - Pf6N2E2_4453 0.34 -0.7 5,184,802 - Pf6N2E2_4453 0.34 +0.7 5,184,802 - Pf6N2E2_4453 0.34 -0.5 5,184,802 - Pf6N2E2_4453 0.34 -0.3 5,184,802 - Pf6N2E2_4453 0.34 -1.0 5,184,802 - Pf6N2E2_4453 0.34 +0.6 5,184,802 - Pf6N2E2_4453 0.34 -0.4 5,184,802 - Pf6N2E2_4453 0.34 -1.2 5,184,802 - Pf6N2E2_4453 0.34 -1.2 5,184,802 - Pf6N2E2_4453 0.34 -0.4 5,184,802 - Pf6N2E2_4453 0.34 -1.0 5,184,802 - Pf6N2E2_4453 0.34 +0.3 5,184,802 - Pf6N2E2_4453 0.34 -1.0 5,184,802 - Pf6N2E2_4453 0.34 +0.7 5,184,802 - Pf6N2E2_4453 0.34 -0.9 5,184,802 - Pf6N2E2_4453 0.34 -0.2 5,184,802 - Pf6N2E2_4453 0.34 -0.4 5,184,802 - Pf6N2E2_4453 0.34 -1.9 5,184,802 - Pf6N2E2_4453 0.34 +0.2 5,184,802 - Pf6N2E2_4453 0.34 -1.1 5,184,873 + Pf6N2E2_4453 0.37 -1.7 5,184,873 + Pf6N2E2_4453 0.37 +0.4 5,184,874 - Pf6N2E2_4453 0.37 -0.7 5,185,029 + Pf6N2E2_4453 0.44 -0.1 5,185,030 - Pf6N2E2_4453 0.44 -0.5 5,185,068 + Pf6N2E2_4453 0.46 -1.6 5,185,068 + Pf6N2E2_4453 0.46 -1.0 5,185,069 - Pf6N2E2_4453 0.46 -0.8 5,185,144 - Pf6N2E2_4453 0.49 -0.5 5,185,321 - Pf6N2E2_4453 0.57 -0.7 5,185,479 + Pf6N2E2_4453 0.64 -3.1 5,185,479 + Pf6N2E2_4453 0.64 -0.8 5,185,479 + Pf6N2E2_4453 0.64 -0.1 5,185,479 + Pf6N2E2_4453 0.64 -2.1 5,185,480 - Pf6N2E2_4453 0.64 -0.8 5,185,480 - Pf6N2E2_4453 0.64 -0.6 5,185,480 - Pf6N2E2_4453 0.64 -0.0 5,185,480 - Pf6N2E2_4453 0.64 -0.9 5,185,480 - Pf6N2E2_4453 0.64 -0.4 5,185,487 - Pf6N2E2_4453 0.65 -1.6 5,185,578 + Pf6N2E2_4453 0.69 -0.5 5,185,578 + Pf6N2E2_4453 0.69 -0.7 5,185,578 + Pf6N2E2_4453 0.69 -1.0 5,185,579 - Pf6N2E2_4453 0.69 -0.2 5,185,579 - Pf6N2E2_4453 0.69 -0.9 5,185,579 - Pf6N2E2_4453 0.69 -0.2 5,185,602 + Pf6N2E2_4453 0.70 +0.5 5,185,603 - Pf6N2E2_4453 0.70 -0.6 5,185,611 + Pf6N2E2_4453 0.70 +0.0 5,185,611 + Pf6N2E2_4453 0.70 -0.2 5,185,611 + Pf6N2E2_4453 0.70 -0.8 5,185,612 - Pf6N2E2_4453 0.70 -0.2 5,185,612 - Pf6N2E2_4453 0.70 -0.1 5,185,612 - Pf6N2E2_4453 0.70 -0.7 5,185,796 + Pf6N2E2_4453 0.78 +0.2 5,185,848 + Pf6N2E2_4453 0.81 +0.3 5,185,848 + Pf6N2E2_4453 0.81 -0.9 5,185,849 - Pf6N2E2_4453 0.81 -1.0 5,185,849 - Pf6N2E2_4453 0.81 -0.1 5,185,849 - Pf6N2E2_4453 0.81 +0.1 5,185,945 + Pf6N2E2_4453 0.85 -0.1 5,185,945 + Pf6N2E2_4453 0.85 +0.3 5,185,945 + Pf6N2E2_4453 0.85 -0.7 5,185,945 + Pf6N2E2_4453 0.85 -0.8 5,185,945 + Pf6N2E2_4453 0.85 -1.3 5,185,945 + Pf6N2E2_4453 0.85 -1.6 5,185,945 + Pf6N2E2_4453 0.85 +0.2 5,185,945 + Pf6N2E2_4453 0.85 -0.1 5,185,945 + Pf6N2E2_4453 0.85 -0.9 5,185,946 - Pf6N2E2_4453 0.85 -1.0 5,185,946 - Pf6N2E2_4453 0.85 -0.4 5,185,946 - Pf6N2E2_4453 0.85 -0.6 5,185,946 - Pf6N2E2_4453 0.85 -0.9 5,185,946 - Pf6N2E2_4453 0.85 -1.2 5,185,946 - Pf6N2E2_4453 0.85 -1.4 5,185,946 - Pf6N2E2_4453 0.85 -2.0 5,185,946 - Pf6N2E2_4453 0.85 -0.3 5,185,946 - Pf6N2E2_4453 0.85 +0.2 5,185,976 + Pf6N2E2_4453 0.87 -2.1 5,185,976 + Pf6N2E2_4453 0.87 -0.8 5,185,977 - Pf6N2E2_4453 0.87 -1.4 5,185,977 - Pf6N2E2_4453 0.87 -1.7 5,185,989 + Pf6N2E2_4453 0.87 +0.2 5,185,989 + Pf6N2E2_4453 0.87 -0.5 5,185,989 + Pf6N2E2_4453 0.87 -0.2 5,185,989 + Pf6N2E2_4453 0.87 -0.7 5,185,990 - Pf6N2E2_4453 0.87 -0.3 5,186,130 + -1.1 5,186,130 + +1.7 5,186,130 + -0.7 5,186,130 + -1.1 5,186,130 + -0.4 5,186,130 + -0.8 5,186,178 + -0.8 5,186,178 + -1.3 5,186,178 + -0.8 5,186,178 + -2.4 5,186,178 + -1.6 5,186,178 + -0.3 5,186,179 - +0.3 5,186,401 + +1.0 5,186,401 + +0.3 5,186,401 + -1.1 5,186,401 + +0.6 5,186,401 + +0.4 5,186,401 + -0.2 5,186,401 + +1.0 5,186,401 + -0.2 5,186,401 + -0.3 5,186,401 + +0.9 5,186,401 + +0.6 5,186,401 + +0.4 5,186,402 - -0.8 5,186,402 - +0.1 5,186,402 - -0.9 5,186,402 - +0.7 5,186,402 - -0.4 5,186,402 - -0.3 5,186,670 - Pf6N2E2_4454 0.17 +0.7 5,186,767 + Pf6N2E2_4454 0.23 +2.7 5,186,768 - Pf6N2E2_4454 0.23 +2.7 5,186,768 - Pf6N2E2_4454 0.23 +1.0 5,186,786 - Pf6N2E2_4454 0.24 +2.4 5,186,791 + Pf6N2E2_4454 0.25 +1.9 5,187,010 - Pf6N2E2_4454 0.39 +1.3 5,187,016 + Pf6N2E2_4454 0.40 +2.0 5,187,017 - Pf6N2E2_4454 0.40 +2.0
Or see this region's nucleotide sequence