Experiment: D-Glucosamine Hydrochloride (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4391 and Pf6N2E2_4392 overlap by 29 nucleotides Pf6N2E2_4392 and Pf6N2E2_4393 are separated by 97 nucleotides
Pf6N2E2_4391 - D-amino acid dehydrogenase small subunit (EC 1.4.99.1), at 5,119,527 to 5,120,831
_4391
Pf6N2E2_4392 - Translation initiation inhibitor, at 5,120,803 to 5,121,156
_4392
Pf6N2E2_4393 - Alanine racemase (EC 5.1.1.1), at 5,121,254 to 5,122,327
_4393
Position (kb) 5120
5121
5122 Strain fitness (log2 ratio) -1
0
1 at 5119.867 kb on - strand, within Pf6N2E2_4391 at 5119.914 kb on + strand, within Pf6N2E2_4391 at 5119.914 kb on + strand, within Pf6N2E2_4391 at 5120.023 kb on + strand, within Pf6N2E2_4391 at 5120.135 kb on + strand, within Pf6N2E2_4391 at 5120.292 kb on + strand, within Pf6N2E2_4391 at 5120.352 kb on + strand, within Pf6N2E2_4391 at 5120.352 kb on + strand, within Pf6N2E2_4391 at 5120.418 kb on + strand, within Pf6N2E2_4391 at 5120.418 kb on + strand, within Pf6N2E2_4391 at 5120.418 kb on + strand, within Pf6N2E2_4391 at 5120.418 kb on + strand, within Pf6N2E2_4391 at 5120.419 kb on - strand, within Pf6N2E2_4391 at 5120.419 kb on - strand, within Pf6N2E2_4391 at 5120.419 kb on - strand, within Pf6N2E2_4391 at 5120.419 kb on - strand, within Pf6N2E2_4391 at 5120.419 kb on - strand, within Pf6N2E2_4391 at 5120.420 kb on + strand, within Pf6N2E2_4391 at 5120.420 kb on + strand, within Pf6N2E2_4391 at 5120.762 kb on + strand at 5120.762 kb on + strand at 5120.762 kb on + strand at 5120.763 kb on - strand at 5120.869 kb on + strand, within Pf6N2E2_4392 at 5121.001 kb on + strand, within Pf6N2E2_4392 at 5121.154 kb on + strand at 5121.158 kb on + strand at 5121.158 kb on + strand at 5121.158 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (C) remove 5,119,867 - Pf6N2E2_4391 0.26 +1.0 5,119,914 + Pf6N2E2_4391 0.30 -0.5 5,119,914 + Pf6N2E2_4391 0.30 +0.2 5,120,023 + Pf6N2E2_4391 0.38 -1.0 5,120,135 + Pf6N2E2_4391 0.47 -0.1 5,120,292 + Pf6N2E2_4391 0.59 +0.0 5,120,352 + Pf6N2E2_4391 0.63 -1.1 5,120,352 + Pf6N2E2_4391 0.63 -0.8 5,120,418 + Pf6N2E2_4391 0.68 -0.6 5,120,418 + Pf6N2E2_4391 0.68 -1.5 5,120,418 + Pf6N2E2_4391 0.68 -0.2 5,120,418 + Pf6N2E2_4391 0.68 -1.1 5,120,419 - Pf6N2E2_4391 0.68 +1.2 5,120,419 - Pf6N2E2_4391 0.68 +0.5 5,120,419 - Pf6N2E2_4391 0.68 -0.4 5,120,419 - Pf6N2E2_4391 0.68 +0.6 5,120,419 - Pf6N2E2_4391 0.68 +0.1 5,120,420 + Pf6N2E2_4391 0.68 +0.6 5,120,420 + Pf6N2E2_4391 0.68 -0.5 5,120,762 + -0.4 5,120,762 + +0.5 5,120,762 + +0.9 5,120,763 - -0.1 5,120,869 + Pf6N2E2_4392 0.19 -0.5 5,121,001 + Pf6N2E2_4392 0.56 -0.6 5,121,154 + +0.6 5,121,158 + -0.1 5,121,158 + +1.4 5,121,158 + +0.5
Or see this region's nucleotide sequence