Experiment: D-Glucosamine Hydrochloride (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3276 and Pf6N2E2_3277 overlap by 8 nucleotides Pf6N2E2_3277 and Pf6N2E2_3278 are separated by 284 nucleotides
Pf6N2E2_3276 - Fumarate hydratase class II (EC 4.2.1.2), at 3,779,056 to 3,780,432
_3276
Pf6N2E2_3277 - FagA protein, at 3,780,425 to 3,780,820
_3277
Pf6N2E2_3278 - TldE protein, part of TldE/TldD proteolytic complex, at 3,781,105 to 3,782,463
_3278
Position (kb)
3780
3781 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3779.950 kb on + strand, within Pf6N2E2_3276 at 3779.950 kb on + strand, within Pf6N2E2_3276 at 3779.950 kb on + strand, within Pf6N2E2_3276 at 3779.951 kb on - strand, within Pf6N2E2_3276 at 3779.951 kb on - strand, within Pf6N2E2_3276 at 3780.194 kb on - strand, within Pf6N2E2_3276 at 3780.253 kb on + strand, within Pf6N2E2_3276 at 3780.254 kb on - strand, within Pf6N2E2_3276 at 3780.254 kb on - strand, within Pf6N2E2_3276 at 3780.254 kb on - strand, within Pf6N2E2_3276 at 3780.315 kb on + strand at 3780.315 kb on + strand at 3780.316 kb on - strand at 3780.316 kb on - strand at 3780.316 kb on - strand at 3780.424 kb on - strand at 3780.424 kb on - strand at 3780.424 kb on - strand at 3780.426 kb on + strand at 3780.426 kb on + strand at 3780.426 kb on + strand at 3780.426 kb on + strand at 3780.427 kb on - strand at 3780.427 kb on - strand at 3780.427 kb on - strand at 3780.427 kb on - strand at 3780.427 kb on - strand at 3780.457 kb on + strand at 3780.458 kb on - strand at 3780.458 kb on - strand at 3780.458 kb on - strand at 3780.714 kb on + strand, within Pf6N2E2_3277 at 3780.714 kb on + strand, within Pf6N2E2_3277 at 3780.715 kb on - strand, within Pf6N2E2_3277 at 3780.792 kb on + strand at 3780.792 kb on + strand at 3780.793 kb on - strand at 3780.793 kb on - strand at 3780.793 kb on - strand at 3780.950 kb on + strand at 3781.189 kb on + strand at 3781.216 kb on - strand at 3781.295 kb on + strand, within Pf6N2E2_3278 at 3781.296 kb on - strand, within Pf6N2E2_3278 at 3781.296 kb on - strand, within Pf6N2E2_3278 at 3781.410 kb on + strand, within Pf6N2E2_3278 at 3781.411 kb on - strand, within Pf6N2E2_3278 at 3781.463 kb on + strand, within Pf6N2E2_3278 at 3781.463 kb on + strand, within Pf6N2E2_3278 at 3781.463 kb on + strand, within Pf6N2E2_3278 at 3781.464 kb on - strand, within Pf6N2E2_3278 at 3781.478 kb on + strand, within Pf6N2E2_3278 at 3781.478 kb on + strand, within Pf6N2E2_3278 at 3781.478 kb on + strand, within Pf6N2E2_3278 at 3781.478 kb on + strand, within Pf6N2E2_3278 at 3781.478 kb on + strand, within Pf6N2E2_3278 at 3781.478 kb on + strand, within Pf6N2E2_3278 at 3781.479 kb on - strand, within Pf6N2E2_3278 at 3781.479 kb on - strand, within Pf6N2E2_3278 at 3781.517 kb on + strand, within Pf6N2E2_3278 at 3781.517 kb on + strand, within Pf6N2E2_3278 at 3781.518 kb on - strand, within Pf6N2E2_3278 at 3781.518 kb on - strand, within Pf6N2E2_3278 at 3781.518 kb on - strand, within Pf6N2E2_3278
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (C) remove 3,779,950 + Pf6N2E2_3276 0.65 +1.3 3,779,950 + Pf6N2E2_3276 0.65 +0.6 3,779,950 + Pf6N2E2_3276 0.65 -3.0 3,779,951 - Pf6N2E2_3276 0.65 +0.1 3,779,951 - Pf6N2E2_3276 0.65 +0.3 3,780,194 - Pf6N2E2_3276 0.83 +0.2 3,780,253 + Pf6N2E2_3276 0.87 +0.6 3,780,254 - Pf6N2E2_3276 0.87 -0.3 3,780,254 - Pf6N2E2_3276 0.87 +0.6 3,780,254 - Pf6N2E2_3276 0.87 +0.3 3,780,315 + +0.6 3,780,315 + +0.6 3,780,316 - +1.1 3,780,316 - +0.3 3,780,316 - -0.1 3,780,424 - +0.0 3,780,424 - -0.2 3,780,424 - +0.3 3,780,426 + -0.4 3,780,426 + -0.2 3,780,426 + -2.0 3,780,426 + +0.3 3,780,427 - +1.1 3,780,427 - +0.8 3,780,427 - -2.4 3,780,427 - -0.1 3,780,427 - +0.3 3,780,457 + +0.9 3,780,458 - +0.0 3,780,458 - +0.6 3,780,458 - -0.3 3,780,714 + Pf6N2E2_3277 0.73 -1.5 3,780,714 + Pf6N2E2_3277 0.73 -0.9 3,780,715 - Pf6N2E2_3277 0.73 -1.5 3,780,792 + +0.3 3,780,792 + +0.5 3,780,793 - +0.6 3,780,793 - +0.2 3,780,793 - +0.7 3,780,950 + -0.5 3,781,189 + +0.1 3,781,216 - +0.7 3,781,295 + Pf6N2E2_3278 0.14 -0.3 3,781,296 - Pf6N2E2_3278 0.14 +1.4 3,781,296 - Pf6N2E2_3278 0.14 -0.5 3,781,410 + Pf6N2E2_3278 0.22 +1.1 3,781,411 - Pf6N2E2_3278 0.23 +0.3 3,781,463 + Pf6N2E2_3278 0.26 -0.4 3,781,463 + Pf6N2E2_3278 0.26 +0.3 3,781,463 + Pf6N2E2_3278 0.26 +0.4 3,781,464 - Pf6N2E2_3278 0.26 +0.7 3,781,478 + Pf6N2E2_3278 0.27 -0.0 3,781,478 + Pf6N2E2_3278 0.27 -0.2 3,781,478 + Pf6N2E2_3278 0.27 -0.0 3,781,478 + Pf6N2E2_3278 0.27 +0.4 3,781,478 + Pf6N2E2_3278 0.27 +1.1 3,781,478 + Pf6N2E2_3278 0.27 -0.7 3,781,479 - Pf6N2E2_3278 0.28 -0.1 3,781,479 - Pf6N2E2_3278 0.28 -0.2 3,781,517 + Pf6N2E2_3278 0.30 +0.3 3,781,517 + Pf6N2E2_3278 0.30 +0.3 3,781,518 - Pf6N2E2_3278 0.30 -0.2 3,781,518 - Pf6N2E2_3278 0.30 -1.0 3,781,518 - Pf6N2E2_3278 0.30 +0.6
Or see this region's nucleotide sequence