Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_2984

Experiment: D-Glucosamine Hydrochloride (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_2982 and Pf6N2E2_2983 are separated by 108 nucleotidesPf6N2E2_2983 and Pf6N2E2_2984 are separated by 61 nucleotidesPf6N2E2_2984 and Pf6N2E2_2985 are separated by 4 nucleotidesPf6N2E2_2985 and Pf6N2E2_2986 are separated by 72 nucleotides Pf6N2E2_2982 - COG1720: Uncharacterized conserved protein, at 3,477,377 to 3,478,075 _2982 Pf6N2E2_2983 - Ferredoxin--NADP(+) reductase (EC 1.18.1.2), at 3,478,184 to 3,478,963 _2983 Pf6N2E2_2984 - LysR family transcriptional regulator PA3398, at 3,479,025 to 3,480,011 _2984 Pf6N2E2_2985 - Diacylglycerol kinase (EC 2.7.1.107), at 3,480,016 to 3,480,378 _2985 Pf6N2E2_2986 - Transcriptional regulator, LuxR family, at 3,480,451 to 3,481,104 _2986 Position (kb) 3479 3480 3481Strain fitness (log2 ratio) -2 -1 0 1 2at 3478.066 kb on + strandat 3478.066 kb on + strandat 3478.185 kb on + strandat 3478.185 kb on + strandat 3478.185 kb on + strandat 3478.185 kb on + strandat 3478.186 kb on - strandat 3478.186 kb on - strandat 3479.094 kb on - strandat 3479.395 kb on - strand, within Pf6N2E2_2984at 3479.500 kb on - strand, within Pf6N2E2_2984at 3479.904 kb on - strand, within Pf6N2E2_2984at 3479.914 kb on - strandat 3480.064 kb on + strand, within Pf6N2E2_2985at 3480.064 kb on + strand, within Pf6N2E2_2985at 3480.065 kb on - strand, within Pf6N2E2_2985at 3480.065 kb on - strand, within Pf6N2E2_2985at 3480.065 kb on - strand, within Pf6N2E2_2985at 3480.065 kb on - strand, within Pf6N2E2_2985at 3480.366 kb on + strandat 3480.366 kb on + strandat 3480.367 kb on - strandat 3480.378 kb on + strandat 3480.378 kb on + strandat 3480.378 kb on + strandat 3480.378 kb on + strandat 3480.378 kb on + strandat 3480.378 kb on + strandat 3480.378 kb on + strandat 3480.378 kb on + strandat 3480.378 kb on + strandat 3480.378 kb on + strandat 3480.379 kb on - strandat 3480.379 kb on - strandat 3480.379 kb on - strandat 3480.379 kb on - strandat 3480.379 kb on - strandat 3480.379 kb on - strandat 3480.379 kb on - strandat 3480.379 kb on - strandat 3480.379 kb on - strandat 3480.414 kb on + strandat 3480.415 kb on - strandat 3480.452 kb on + strandat 3480.452 kb on + strandat 3480.452 kb on + strandat 3480.452 kb on + strandat 3480.452 kb on + strandat 3480.452 kb on + strandat 3480.453 kb on - strandat 3480.453 kb on - strandat 3480.453 kb on - strandat 3480.602 kb on - strand, within Pf6N2E2_2986at 3480.787 kb on + strand, within Pf6N2E2_2986at 3480.787 kb on + strand, within Pf6N2E2_2986at 3480.876 kb on - strand, within Pf6N2E2_2986at 3480.912 kb on - strand, within Pf6N2E2_2986at 3480.912 kb on - strand, within Pf6N2E2_2986

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (C)
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3,478,066 + -1.9
3,478,066 + -1.4
3,478,185 + +1.2
3,478,185 + +0.8
3,478,185 + +1.1
3,478,185 + +0.6
3,478,186 - -2.6
3,478,186 - -0.1
3,479,094 - -0.4
3,479,395 - Pf6N2E2_2984 0.37 -1.1
3,479,500 - Pf6N2E2_2984 0.48 -0.2
3,479,904 - Pf6N2E2_2984 0.89 -0.5
3,479,914 - -0.2
3,480,064 + Pf6N2E2_2985 0.13 +0.7
3,480,064 + Pf6N2E2_2985 0.13 -0.5
3,480,065 - Pf6N2E2_2985 0.13 -0.5
3,480,065 - Pf6N2E2_2985 0.13 -0.1
3,480,065 - Pf6N2E2_2985 0.13 +0.4
3,480,065 - Pf6N2E2_2985 0.13 +0.5
3,480,366 + +0.1
3,480,366 + +0.2
3,480,367 - +0.6
3,480,378 + -1.3
3,480,378 + -0.9
3,480,378 + +1.3
3,480,378 + +0.6
3,480,378 + +0.1
3,480,378 + +0.4
3,480,378 + -0.2
3,480,378 + +1.4
3,480,378 + +0.7
3,480,378 + +0.7
3,480,379 - -0.1
3,480,379 - -0.3
3,480,379 - +0.8
3,480,379 - +1.1
3,480,379 - +0.2
3,480,379 - -1.9
3,480,379 - -0.4
3,480,379 - +0.1
3,480,379 - -0.3
3,480,414 + +0.0
3,480,415 - +0.5
3,480,452 + -1.8
3,480,452 + +0.7
3,480,452 + +1.5
3,480,452 + -0.7
3,480,452 + +1.4
3,480,452 + +0.1
3,480,453 - -0.0
3,480,453 - +0.1
3,480,453 - +0.1
3,480,602 - Pf6N2E2_2986 0.23 +0.0
3,480,787 + Pf6N2E2_2986 0.51 +1.4
3,480,787 + Pf6N2E2_2986 0.51 +2.0
3,480,876 - Pf6N2E2_2986 0.65 +0.9
3,480,912 - Pf6N2E2_2986 0.70 +0.2
3,480,912 - Pf6N2E2_2986 0.70 +1.5

Or see this region's nucleotide sequence