Experiment: D-Glucosamine Hydrochloride (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2297 and Pf6N2E2_2298 are separated by 386 nucleotides Pf6N2E2_2298 and Pf6N2E2_2299 overlap by 4 nucleotides Pf6N2E2_2299 and Pf6N2E2_2300 are separated by 187 nucleotides
Pf6N2E2_2297 - Erythronate-4-phosphate dehydrogenase (EC 1.1.1.290), at 2,719,560 to 2,720,702
_2297
Pf6N2E2_2298 - Multi antimicrobial extrusion protein (Na(+)/drug antiporter), MATE family of MDR efflux pumps, at 2,721,089 to 2,722,423
_2298
Pf6N2E2_2299 - tRNA 5-methylaminomethyl-2-thiouridine synthase TusA, at 2,722,420 to 2,722,671
_2299
Pf6N2E2_2300 - LSU rRNA 2'-O-methyl-C2498 methyltransferase RlmM, at 2,722,859 to 2,723,932
_2300
Position (kb)
2721
2722
2723 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2720.733 kb on + strand at 2720.734 kb on - strand at 2720.745 kb on + strand at 2720.912 kb on + strand at 2720.912 kb on + strand at 2720.912 kb on + strand at 2720.912 kb on + strand at 2720.913 kb on - strand at 2720.913 kb on - strand at 2720.931 kb on - strand at 2720.931 kb on - strand at 2720.976 kb on + strand at 2720.977 kb on - strand at 2720.977 kb on - strand at 2720.998 kb on - strand at 2720.998 kb on - strand at 2721.031 kb on - strand at 2721.443 kb on + strand, within Pf6N2E2_2298 at 2721.443 kb on + strand, within Pf6N2E2_2298 at 2721.444 kb on - strand, within Pf6N2E2_2298 at 2721.444 kb on - strand, within Pf6N2E2_2298 at 2721.485 kb on + strand, within Pf6N2E2_2298 at 2721.485 kb on + strand, within Pf6N2E2_2298 at 2721.486 kb on - strand, within Pf6N2E2_2298 at 2721.492 kb on + strand, within Pf6N2E2_2298 at 2721.492 kb on + strand, within Pf6N2E2_2298 at 2721.493 kb on - strand, within Pf6N2E2_2298 at 2721.493 kb on - strand, within Pf6N2E2_2298 at 2721.581 kb on + strand, within Pf6N2E2_2298 at 2721.581 kb on + strand, within Pf6N2E2_2298 at 2721.581 kb on + strand, within Pf6N2E2_2298 at 2721.581 kb on + strand, within Pf6N2E2_2298 at 2721.581 kb on + strand, within Pf6N2E2_2298 at 2721.581 kb on + strand, within Pf6N2E2_2298 at 2721.581 kb on + strand, within Pf6N2E2_2298 at 2721.582 kb on - strand, within Pf6N2E2_2298 at 2721.582 kb on - strand, within Pf6N2E2_2298 at 2721.582 kb on - strand, within Pf6N2E2_2298 at 2721.582 kb on - strand, within Pf6N2E2_2298 at 2721.582 kb on - strand, within Pf6N2E2_2298 at 2721.593 kb on + strand, within Pf6N2E2_2298 at 2721.593 kb on + strand, within Pf6N2E2_2298 at 2721.593 kb on + strand, within Pf6N2E2_2298 at 2721.593 kb on + strand, within Pf6N2E2_2298 at 2721.594 kb on - strand, within Pf6N2E2_2298 at 2721.708 kb on - strand, within Pf6N2E2_2298 at 2721.708 kb on - strand, within Pf6N2E2_2298 at 2721.708 kb on - strand, within Pf6N2E2_2298 at 2721.920 kb on + strand, within Pf6N2E2_2298 at 2722.029 kb on - strand, within Pf6N2E2_2298 at 2722.085 kb on + strand, within Pf6N2E2_2298 at 2722.085 kb on + strand, within Pf6N2E2_2298 at 2722.085 kb on + strand, within Pf6N2E2_2298 at 2722.086 kb on - strand, within Pf6N2E2_2298 at 2722.086 kb on - strand, within Pf6N2E2_2298 at 2722.086 kb on - strand, within Pf6N2E2_2298 at 2722.086 kb on - strand, within Pf6N2E2_2298 at 2722.086 kb on - strand, within Pf6N2E2_2298 at 2722.134 kb on - strand, within Pf6N2E2_2298 at 2722.677 kb on - strand at 2722.677 kb on - strand at 2722.926 kb on + strand at 2722.927 kb on - strand at 2722.927 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (C) remove 2,720,733 + +0.1 2,720,734 - +0.4 2,720,745 + +0.3 2,720,912 + +1.2 2,720,912 + +1.3 2,720,912 + +0.7 2,720,912 + +0.8 2,720,913 - -0.3 2,720,913 - +0.5 2,720,931 - +0.3 2,720,931 - +0.3 2,720,976 + -0.8 2,720,977 - -0.8 2,720,977 - +0.8 2,720,998 - -0.1 2,720,998 - +0.2 2,721,031 - +0.9 2,721,443 + Pf6N2E2_2298 0.27 +1.7 2,721,443 + Pf6N2E2_2298 0.27 +0.5 2,721,444 - Pf6N2E2_2298 0.27 +1.4 2,721,444 - Pf6N2E2_2298 0.27 -0.5 2,721,485 + Pf6N2E2_2298 0.30 +0.4 2,721,485 + Pf6N2E2_2298 0.30 +1.7 2,721,486 - Pf6N2E2_2298 0.30 +0.7 2,721,492 + Pf6N2E2_2298 0.30 +0.2 2,721,492 + Pf6N2E2_2298 0.30 -0.5 2,721,493 - Pf6N2E2_2298 0.30 +0.5 2,721,493 - Pf6N2E2_2298 0.30 -0.1 2,721,581 + Pf6N2E2_2298 0.37 -0.1 2,721,581 + Pf6N2E2_2298 0.37 +1.2 2,721,581 + Pf6N2E2_2298 0.37 -0.1 2,721,581 + Pf6N2E2_2298 0.37 +1.7 2,721,581 + Pf6N2E2_2298 0.37 +1.3 2,721,581 + Pf6N2E2_2298 0.37 +0.3 2,721,581 + Pf6N2E2_2298 0.37 +0.9 2,721,582 - Pf6N2E2_2298 0.37 +0.6 2,721,582 - Pf6N2E2_2298 0.37 +1.0 2,721,582 - Pf6N2E2_2298 0.37 +1.0 2,721,582 - Pf6N2E2_2298 0.37 +0.8 2,721,582 - Pf6N2E2_2298 0.37 +1.9 2,721,593 + Pf6N2E2_2298 0.38 +0.7 2,721,593 + Pf6N2E2_2298 0.38 +0.4 2,721,593 + Pf6N2E2_2298 0.38 +0.7 2,721,593 + Pf6N2E2_2298 0.38 -1.2 2,721,594 - Pf6N2E2_2298 0.38 -0.6 2,721,708 - Pf6N2E2_2298 0.46 +0.1 2,721,708 - Pf6N2E2_2298 0.46 +0.4 2,721,708 - Pf6N2E2_2298 0.46 +0.7 2,721,920 + Pf6N2E2_2298 0.62 +1.8 2,722,029 - Pf6N2E2_2298 0.70 -1.0 2,722,085 + Pf6N2E2_2298 0.75 -0.2 2,722,085 + Pf6N2E2_2298 0.75 -0.3 2,722,085 + Pf6N2E2_2298 0.75 -0.0 2,722,086 - Pf6N2E2_2298 0.75 -1.9 2,722,086 - Pf6N2E2_2298 0.75 +0.2 2,722,086 - Pf6N2E2_2298 0.75 +1.9 2,722,086 - Pf6N2E2_2298 0.75 +0.3 2,722,086 - Pf6N2E2_2298 0.75 -0.9 2,722,134 - Pf6N2E2_2298 0.78 +2.2 2,722,677 - -1.2 2,722,677 - +0.7 2,722,926 + +1.7 2,722,927 - +0.2 2,722,927 - +0.2
Or see this region's nucleotide sequence