Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_201

Experiment: D-Glucosamine Hydrochloride (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_200 and Pf6N2E2_201 overlap by 10 nucleotidesPf6N2E2_201 and Pf6N2E2_202 overlap by 4 nucleotides Pf6N2E2_200 - Possible glycosyltransferase, at 250,861 to 251,967 _200 Pf6N2E2_201 - UDP-N-acetylglucosamine 2-epimerase (EC 5.1.3.14), at 251,958 to 253,067 _201 Pf6N2E2_202 - 4Fe-4S ferredoxin, iron-sulfur binding, at 253,064 to 254,650 _202 Position (kb) 251 252 253 254Strain fitness (log2 ratio) -2 -1 0 1at 251.087 kb on + strand, within Pf6N2E2_200at 251.088 kb on - strand, within Pf6N2E2_200at 251.222 kb on - strand, within Pf6N2E2_200at 251.222 kb on - strand, within Pf6N2E2_200at 251.422 kb on + strand, within Pf6N2E2_200at 251.423 kb on - strand, within Pf6N2E2_200at 251.476 kb on + strand, within Pf6N2E2_200at 251.476 kb on + strand, within Pf6N2E2_200at 251.477 kb on - strand, within Pf6N2E2_200at 251.544 kb on - strand, within Pf6N2E2_200at 251.574 kb on - strand, within Pf6N2E2_200at 251.870 kb on - strandat 251.954 kb on + strandat 251.954 kb on + strandat 251.954 kb on + strandat 251.955 kb on - strandat 251.955 kb on - strandat 251.955 kb on - strandat 251.977 kb on + strandat 251.977 kb on + strandat 252.056 kb on - strandat 252.241 kb on + strand, within Pf6N2E2_201at 252.242 kb on - strand, within Pf6N2E2_201at 252.242 kb on - strand, within Pf6N2E2_201at 252.242 kb on - strand, within Pf6N2E2_201at 252.274 kb on + strand, within Pf6N2E2_201at 252.274 kb on + strand, within Pf6N2E2_201at 252.274 kb on + strand, within Pf6N2E2_201at 252.688 kb on - strand, within Pf6N2E2_201at 252.688 kb on - strand, within Pf6N2E2_201at 253.053 kb on + strandat 253.054 kb on - strandat 253.054 kb on - strandat 253.054 kb on - strandat 253.091 kb on + strandat 253.091 kb on + strandat 253.091 kb on + strandat 253.183 kb on + strandat 253.183 kb on + strandat 253.183 kb on + strandat 253.184 kb on - strandat 253.338 kb on + strand, within Pf6N2E2_202at 253.338 kb on + strand, within Pf6N2E2_202at 253.339 kb on - strand, within Pf6N2E2_202at 253.472 kb on - strand, within Pf6N2E2_202at 253.472 kb on - strand, within Pf6N2E2_202at 253.472 kb on - strand, within Pf6N2E2_202at 253.604 kb on - strand, within Pf6N2E2_202at 253.941 kb on + strand, within Pf6N2E2_202at 253.941 kb on + strand, within Pf6N2E2_202at 253.942 kb on - strand, within Pf6N2E2_202at 253.942 kb on - strand, within Pf6N2E2_202at 253.942 kb on - strand, within Pf6N2E2_202at 253.942 kb on - strand, within Pf6N2E2_202at 253.942 kb on - strand, within Pf6N2E2_202at 253.942 kb on - strand, within Pf6N2E2_202at 253.942 kb on - strand, within Pf6N2E2_202at 253.942 kb on - strand, within Pf6N2E2_202at 253.942 kb on - strand, within Pf6N2E2_202at 253.942 kb on - strand, within Pf6N2E2_202

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (C)
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251,087 + Pf6N2E2_200 0.20 +0.4
251,088 - Pf6N2E2_200 0.21 -0.4
251,222 - Pf6N2E2_200 0.33 +0.6
251,222 - Pf6N2E2_200 0.33 -0.4
251,422 + Pf6N2E2_200 0.51 +1.1
251,423 - Pf6N2E2_200 0.51 -0.7
251,476 + Pf6N2E2_200 0.56 -0.0
251,476 + Pf6N2E2_200 0.56 +0.7
251,477 - Pf6N2E2_200 0.56 +0.8
251,544 - Pf6N2E2_200 0.62 +0.4
251,574 - Pf6N2E2_200 0.64 -0.5
251,870 - -0.8
251,954 + -0.8
251,954 + +0.1
251,954 + -1.1
251,955 - +1.1
251,955 - -0.4
251,955 - -0.2
251,977 + +0.2
251,977 + +0.3
252,056 - +0.5
252,241 + Pf6N2E2_201 0.25 -0.9
252,242 - Pf6N2E2_201 0.26 -2.4
252,242 - Pf6N2E2_201 0.26 +1.2
252,242 - Pf6N2E2_201 0.26 -1.2
252,274 + Pf6N2E2_201 0.28 -0.6
252,274 + Pf6N2E2_201 0.28 -0.9
252,274 + Pf6N2E2_201 0.28 -1.0
252,688 - Pf6N2E2_201 0.66 -0.4
252,688 - Pf6N2E2_201 0.66 -1.3
253,053 + -1.1
253,054 - -0.5
253,054 - +0.2
253,054 - +1.1
253,091 + -0.9
253,091 + -0.6
253,091 + +1.3
253,183 + +0.5
253,183 + +1.1
253,183 + -0.7
253,184 - +1.2
253,338 + Pf6N2E2_202 0.17 -1.4
253,338 + Pf6N2E2_202 0.17 +0.1
253,339 - Pf6N2E2_202 0.17 +0.5
253,472 - Pf6N2E2_202 0.26 -0.3
253,472 - Pf6N2E2_202 0.26 -1.7
253,472 - Pf6N2E2_202 0.26 +0.9
253,604 - Pf6N2E2_202 0.34 +0.3
253,941 + Pf6N2E2_202 0.55 -2.0
253,941 + Pf6N2E2_202 0.55 -0.6
253,942 - Pf6N2E2_202 0.55 +0.2
253,942 - Pf6N2E2_202 0.55 -0.4
253,942 - Pf6N2E2_202 0.55 -0.0
253,942 - Pf6N2E2_202 0.55 +1.2
253,942 - Pf6N2E2_202 0.55 -0.7
253,942 - Pf6N2E2_202 0.55 +0.7
253,942 - Pf6N2E2_202 0.55 -0.2
253,942 - Pf6N2E2_202 0.55 +1.3
253,942 - Pf6N2E2_202 0.55 -0.3
253,942 - Pf6N2E2_202 0.55 +0.8

Or see this region's nucleotide sequence