Experiment: D-Glucosamine Hydrochloride (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1945 and Pf6N2E2_1946 overlap by 4 nucleotides Pf6N2E2_1946 and Pf6N2E2_1947 overlap by 29 nucleotides Pf6N2E2_1947 and Pf6N2E2_1948 are separated by 925 nucleotides
Pf6N2E2_1945 - Pyrroloquinoline-quinone synthase (EC 1.3.3.11), at 2,306,594 to 2,307,346
_1945
Pf6N2E2_1946 - Coenzyme PQQ synthesis protein D, at 2,307,343 to 2,307,618
_1946
Pf6N2E2_1947 - Coenzyme PQQ synthesis protein E, at 2,307,590 to 2,308,759
_1947
Pf6N2E2_1948 - RND efflux system, outer membrane lipoprotein CmeC, at 2,309,685 to 2,311,109
_1948
Position (kb)
2307
2308
2309 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2306.654 kb on + strand at 2306.657 kb on + strand at 2306.690 kb on + strand, within Pf6N2E2_1945 at 2306.690 kb on + strand, within Pf6N2E2_1945 at 2306.690 kb on + strand, within Pf6N2E2_1945 at 2306.690 kb on + strand, within Pf6N2E2_1945 at 2306.690 kb on + strand, within Pf6N2E2_1945 at 2306.690 kb on + strand, within Pf6N2E2_1945 at 2306.691 kb on - strand, within Pf6N2E2_1945 at 2306.691 kb on - strand, within Pf6N2E2_1945 at 2306.691 kb on - strand, within Pf6N2E2_1945 at 2306.691 kb on - strand, within Pf6N2E2_1945 at 2306.691 kb on - strand, within Pf6N2E2_1945 at 2306.691 kb on - strand, within Pf6N2E2_1945 at 2306.691 kb on - strand, within Pf6N2E2_1945 at 2306.691 kb on - strand, within Pf6N2E2_1945 at 2306.691 kb on - strand, within Pf6N2E2_1945 at 2306.748 kb on - strand, within Pf6N2E2_1945 at 2306.941 kb on - strand, within Pf6N2E2_1945 at 2306.972 kb on + strand at 2307.113 kb on + strand, within Pf6N2E2_1945 at 2307.114 kb on - strand, within Pf6N2E2_1945 at 2307.114 kb on - strand, within Pf6N2E2_1945 at 2307.114 kb on - strand, within Pf6N2E2_1945 at 2307.114 kb on - strand, within Pf6N2E2_1945 at 2307.114 kb on - strand, within Pf6N2E2_1945 at 2307.114 kb on - strand, within Pf6N2E2_1945 at 2307.169 kb on + strand, within Pf6N2E2_1945 at 2307.182 kb on + strand, within Pf6N2E2_1945 at 2307.183 kb on - strand, within Pf6N2E2_1945 at 2307.183 kb on - strand, within Pf6N2E2_1945 at 2307.183 kb on - strand, within Pf6N2E2_1945 at 2307.183 kb on - strand, within Pf6N2E2_1945 at 2307.183 kb on - strand, within Pf6N2E2_1945 at 2307.296 kb on + strand at 2307.337 kb on + strand at 2307.337 kb on + strand at 2307.338 kb on - strand at 2307.338 kb on - strand at 2307.343 kb on - strand at 2307.343 kb on - strand at 2307.683 kb on + strand at 2307.707 kb on + strand, within Pf6N2E2_1947 at 2307.708 kb on - strand, within Pf6N2E2_1947 at 2307.887 kb on + strand, within Pf6N2E2_1947 at 2307.887 kb on + strand, within Pf6N2E2_1947 at 2307.887 kb on + strand, within Pf6N2E2_1947 at 2307.887 kb on + strand, within Pf6N2E2_1947 at 2307.888 kb on - strand, within Pf6N2E2_1947 at 2307.888 kb on - strand, within Pf6N2E2_1947 at 2308.248 kb on + strand, within Pf6N2E2_1947 at 2308.636 kb on + strand, within Pf6N2E2_1947 at 2308.637 kb on - strand, within Pf6N2E2_1947 at 2308.639 kb on + strand, within Pf6N2E2_1947 at 2308.689 kb on - strand at 2308.689 kb on - strand at 2308.797 kb on - strand at 2308.833 kb on - strand at 2308.895 kb on + strand at 2308.951 kb on - strand at 2309.078 kb on + strand at 2309.110 kb on - strand at 2309.132 kb on + strand at 2309.204 kb on + strand at 2309.204 kb on + strand at 2309.247 kb on + strand at 2309.351 kb on + strand at 2309.351 kb on + strand at 2309.368 kb on - strand at 2309.368 kb on - strand at 2309.389 kb on - strand at 2309.437 kb on + strand at 2309.481 kb on + strand at 2309.526 kb on + strand at 2309.593 kb on + strand at 2309.600 kb on + strand at 2309.600 kb on + strand at 2309.604 kb on + strand at 2309.604 kb on + strand at 2309.694 kb on - strand at 2309.735 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (C) remove 2,306,654 + +0.7 2,306,657 + +1.4 2,306,690 + Pf6N2E2_1945 0.13 +0.7 2,306,690 + Pf6N2E2_1945 0.13 -0.1 2,306,690 + Pf6N2E2_1945 0.13 -0.4 2,306,690 + Pf6N2E2_1945 0.13 -1.1 2,306,690 + Pf6N2E2_1945 0.13 -0.6 2,306,690 + Pf6N2E2_1945 0.13 +1.4 2,306,691 - Pf6N2E2_1945 0.13 +0.0 2,306,691 - Pf6N2E2_1945 0.13 -0.6 2,306,691 - Pf6N2E2_1945 0.13 +0.4 2,306,691 - Pf6N2E2_1945 0.13 +0.4 2,306,691 - Pf6N2E2_1945 0.13 +0.2 2,306,691 - Pf6N2E2_1945 0.13 +1.1 2,306,691 - Pf6N2E2_1945 0.13 -0.1 2,306,691 - Pf6N2E2_1945 0.13 -0.3 2,306,691 - Pf6N2E2_1945 0.13 -0.1 2,306,748 - Pf6N2E2_1945 0.20 +1.0 2,306,941 - Pf6N2E2_1945 0.46 +1.6 2,306,972 + -0.9 2,307,113 + Pf6N2E2_1945 0.69 +1.9 2,307,114 - Pf6N2E2_1945 0.69 +1.2 2,307,114 - Pf6N2E2_1945 0.69 -0.4 2,307,114 - Pf6N2E2_1945 0.69 +0.8 2,307,114 - Pf6N2E2_1945 0.69 +1.6 2,307,114 - Pf6N2E2_1945 0.69 +0.5 2,307,114 - Pf6N2E2_1945 0.69 -1.1 2,307,169 + Pf6N2E2_1945 0.76 +0.1 2,307,182 + Pf6N2E2_1945 0.78 -0.2 2,307,183 - Pf6N2E2_1945 0.78 -0.7 2,307,183 - Pf6N2E2_1945 0.78 -0.3 2,307,183 - Pf6N2E2_1945 0.78 +0.7 2,307,183 - Pf6N2E2_1945 0.78 -1.2 2,307,183 - Pf6N2E2_1945 0.78 +0.1 2,307,296 + +0.5 2,307,337 + +0.2 2,307,337 + -1.5 2,307,338 - +0.6 2,307,338 - +0.9 2,307,343 - -0.4 2,307,343 - +0.3 2,307,683 + -0.2 2,307,707 + Pf6N2E2_1947 0.10 +1.2 2,307,708 - Pf6N2E2_1947 0.10 -1.7 2,307,887 + Pf6N2E2_1947 0.25 -1.0 2,307,887 + Pf6N2E2_1947 0.25 +1.1 2,307,887 + Pf6N2E2_1947 0.25 -0.2 2,307,887 + Pf6N2E2_1947 0.25 -1.0 2,307,888 - Pf6N2E2_1947 0.25 -1.0 2,307,888 - Pf6N2E2_1947 0.25 -1.8 2,308,248 + Pf6N2E2_1947 0.56 -1.2 2,308,636 + Pf6N2E2_1947 0.89 +0.6 2,308,637 - Pf6N2E2_1947 0.89 +1.0 2,308,639 + Pf6N2E2_1947 0.90 -0.0 2,308,689 - +0.3 2,308,689 - -0.3 2,308,797 - +0.7 2,308,833 - +0.7 2,308,895 + +1.7 2,308,951 - -0.5 2,309,078 + +0.5 2,309,110 - +0.1 2,309,132 + +0.5 2,309,204 + +0.3 2,309,204 + +0.2 2,309,247 + +0.3 2,309,351 + -0.5 2,309,351 + -0.5 2,309,368 - +0.4 2,309,368 - -0.1 2,309,389 - +0.7 2,309,437 + -2.2 2,309,481 + -0.3 2,309,526 + +0.1 2,309,593 + -1.2 2,309,600 + -0.1 2,309,600 + +1.5 2,309,604 + -1.0 2,309,604 + -1.1 2,309,694 - +0.2 2,309,735 - +0.2
Or see this region's nucleotide sequence