Experiment: D-Glucosamine Hydrochloride (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1942 and Pf6N2E2_1943 are separated by 267 nucleotides Pf6N2E2_1943 and Pf6N2E2_1944 are separated by 72 nucleotides Pf6N2E2_1944 and Pf6N2E2_1945 are separated by 11 nucleotides Pf6N2E2_1945 and Pf6N2E2_1946 overlap by 4 nucleotides
Pf6N2E2_1942 - Glucose-selective porin OprB, at 2,303,862 to 2,305,190
_1942
Pf6N2E2_1943 - Coenzyme PQQ synthesis protein A, at 2,305,458 to 2,305,598
_1943
Pf6N2E2_1944 - Coenzyme PQQ synthesis protein B, at 2,305,671 to 2,306,582
_1944
Pf6N2E2_1945 - Pyrroloquinoline-quinone synthase (EC 1.3.3.11), at 2,306,594 to 2,307,346
_1945
Pf6N2E2_1946 - Coenzyme PQQ synthesis protein D, at 2,307,343 to 2,307,618
_1946
Position (kb)
2305
2306
2307 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2304.673 kb on - strand, within Pf6N2E2_1942 at 2304.675 kb on + strand, within Pf6N2E2_1942 at 2304.721 kb on + strand, within Pf6N2E2_1942 at 2304.721 kb on + strand, within Pf6N2E2_1942 at 2304.722 kb on - strand, within Pf6N2E2_1942 at 2304.822 kb on + strand, within Pf6N2E2_1942 at 2304.851 kb on + strand, within Pf6N2E2_1942 at 2304.851 kb on + strand, within Pf6N2E2_1942 at 2304.852 kb on - strand, within Pf6N2E2_1942 at 2304.874 kb on + strand, within Pf6N2E2_1942 at 2304.883 kb on - strand, within Pf6N2E2_1942 at 2304.886 kb on - strand, within Pf6N2E2_1942 at 2305.062 kb on + strand at 2305.062 kb on + strand at 2305.063 kb on - strand at 2305.065 kb on + strand at 2305.066 kb on - strand at 2305.066 kb on - strand at 2305.117 kb on + strand at 2305.118 kb on - strand at 2305.188 kb on + strand at 2305.202 kb on + strand at 2305.203 kb on - strand at 2305.343 kb on + strand at 2305.343 kb on + strand at 2305.404 kb on + strand at 2305.405 kb on - strand at 2305.405 kb on - strand at 2305.406 kb on + strand at 2305.406 kb on + strand at 2305.406 kb on + strand at 2305.407 kb on - strand at 2305.429 kb on - strand at 2305.481 kb on + strand, within Pf6N2E2_1943 at 2305.481 kb on + strand, within Pf6N2E2_1943 at 2305.481 kb on + strand, within Pf6N2E2_1943 at 2305.482 kb on - strand, within Pf6N2E2_1943 at 2305.482 kb on - strand, within Pf6N2E2_1943 at 2305.485 kb on + strand, within Pf6N2E2_1943 at 2305.511 kb on + strand, within Pf6N2E2_1943 at 2305.511 kb on + strand, within Pf6N2E2_1943 at 2305.512 kb on - strand, within Pf6N2E2_1943 at 2305.512 kb on - strand, within Pf6N2E2_1943 at 2305.517 kb on + strand, within Pf6N2E2_1943 at 2305.517 kb on + strand, within Pf6N2E2_1943 at 2305.518 kb on - strand, within Pf6N2E2_1943 at 2305.545 kb on + strand, within Pf6N2E2_1943 at 2305.546 kb on - strand, within Pf6N2E2_1943 at 2305.581 kb on + strand, within Pf6N2E2_1943 at 2305.646 kb on + strand at 2305.647 kb on - strand at 2305.687 kb on + strand at 2305.688 kb on - strand at 2305.688 kb on - strand at 2305.688 kb on - strand at 2306.556 kb on + strand at 2306.570 kb on + strand at 2306.570 kb on + strand at 2306.580 kb on + strand at 2306.580 kb on + strand at 2306.580 kb on + strand at 2306.654 kb on + strand at 2306.657 kb on + strand at 2306.690 kb on + strand, within Pf6N2E2_1945 at 2306.690 kb on + strand, within Pf6N2E2_1945 at 2306.690 kb on + strand, within Pf6N2E2_1945 at 2306.690 kb on + strand, within Pf6N2E2_1945 at 2306.690 kb on + strand, within Pf6N2E2_1945 at 2306.690 kb on + strand, within Pf6N2E2_1945 at 2306.691 kb on - strand, within Pf6N2E2_1945 at 2306.691 kb on - strand, within Pf6N2E2_1945 at 2306.691 kb on - strand, within Pf6N2E2_1945 at 2306.691 kb on - strand, within Pf6N2E2_1945 at 2306.691 kb on - strand, within Pf6N2E2_1945 at 2306.691 kb on - strand, within Pf6N2E2_1945 at 2306.691 kb on - strand, within Pf6N2E2_1945 at 2306.691 kb on - strand, within Pf6N2E2_1945 at 2306.691 kb on - strand, within Pf6N2E2_1945 at 2306.748 kb on - strand, within Pf6N2E2_1945 at 2306.941 kb on - strand, within Pf6N2E2_1945 at 2306.972 kb on + strand at 2307.113 kb on + strand, within Pf6N2E2_1945 at 2307.114 kb on - strand, within Pf6N2E2_1945 at 2307.114 kb on - strand, within Pf6N2E2_1945 at 2307.114 kb on - strand, within Pf6N2E2_1945 at 2307.114 kb on - strand, within Pf6N2E2_1945 at 2307.114 kb on - strand, within Pf6N2E2_1945 at 2307.114 kb on - strand, within Pf6N2E2_1945 at 2307.169 kb on + strand, within Pf6N2E2_1945 at 2307.182 kb on + strand, within Pf6N2E2_1945 at 2307.183 kb on - strand, within Pf6N2E2_1945 at 2307.183 kb on - strand, within Pf6N2E2_1945 at 2307.183 kb on - strand, within Pf6N2E2_1945 at 2307.183 kb on - strand, within Pf6N2E2_1945 at 2307.183 kb on - strand, within Pf6N2E2_1945 at 2307.296 kb on + strand at 2307.337 kb on + strand at 2307.337 kb on + strand at 2307.338 kb on - strand at 2307.338 kb on - strand at 2307.343 kb on - strand at 2307.343 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (C) remove 2,304,673 - Pf6N2E2_1942 0.61 +0.1 2,304,675 + Pf6N2E2_1942 0.61 -0.4 2,304,721 + Pf6N2E2_1942 0.65 +0.1 2,304,721 + Pf6N2E2_1942 0.65 -0.4 2,304,722 - Pf6N2E2_1942 0.65 -2.7 2,304,822 + Pf6N2E2_1942 0.72 +0.5 2,304,851 + Pf6N2E2_1942 0.74 -0.1 2,304,851 + Pf6N2E2_1942 0.74 +0.6 2,304,852 - Pf6N2E2_1942 0.74 +0.9 2,304,874 + Pf6N2E2_1942 0.76 +1.0 2,304,883 - Pf6N2E2_1942 0.77 -1.0 2,304,886 - Pf6N2E2_1942 0.77 -0.5 2,305,062 + +0.0 2,305,062 + +0.3 2,305,063 - -0.3 2,305,065 + +1.1 2,305,066 - -0.6 2,305,066 - -0.5 2,305,117 + -0.4 2,305,118 - -0.8 2,305,188 + -2.5 2,305,202 + -0.3 2,305,203 - +0.9 2,305,343 + -1.3 2,305,343 + -1.1 2,305,404 + -0.2 2,305,405 - -0.3 2,305,405 - +2.2 2,305,406 + -0.2 2,305,406 + +0.7 2,305,406 + -0.9 2,305,407 - +2.0 2,305,429 - +0.6 2,305,481 + Pf6N2E2_1943 0.16 +0.0 2,305,481 + Pf6N2E2_1943 0.16 -0.3 2,305,481 + Pf6N2E2_1943 0.16 +0.9 2,305,482 - Pf6N2E2_1943 0.17 -0.2 2,305,482 - Pf6N2E2_1943 0.17 -0.4 2,305,485 + Pf6N2E2_1943 0.19 -0.3 2,305,511 + Pf6N2E2_1943 0.38 -0.4 2,305,511 + Pf6N2E2_1943 0.38 +0.7 2,305,512 - Pf6N2E2_1943 0.38 -0.1 2,305,512 - Pf6N2E2_1943 0.38 +0.6 2,305,517 + Pf6N2E2_1943 0.42 +0.2 2,305,517 + Pf6N2E2_1943 0.42 -0.6 2,305,518 - Pf6N2E2_1943 0.43 +0.9 2,305,545 + Pf6N2E2_1943 0.62 -0.1 2,305,546 - Pf6N2E2_1943 0.62 -0.2 2,305,581 + Pf6N2E2_1943 0.87 -0.5 2,305,646 + +0.4 2,305,647 - -0.2 2,305,687 + +0.8 2,305,688 - +0.1 2,305,688 - +0.1 2,305,688 - +0.6 2,306,556 + -0.8 2,306,570 + +0.5 2,306,570 + -0.3 2,306,580 + -0.1 2,306,580 + +0.1 2,306,580 + +0.7 2,306,654 + +0.7 2,306,657 + +1.4 2,306,690 + Pf6N2E2_1945 0.13 +0.7 2,306,690 + Pf6N2E2_1945 0.13 -0.1 2,306,690 + Pf6N2E2_1945 0.13 -0.4 2,306,690 + Pf6N2E2_1945 0.13 -1.1 2,306,690 + Pf6N2E2_1945 0.13 -0.6 2,306,690 + Pf6N2E2_1945 0.13 +1.4 2,306,691 - Pf6N2E2_1945 0.13 +0.0 2,306,691 - Pf6N2E2_1945 0.13 -0.6 2,306,691 - Pf6N2E2_1945 0.13 +0.4 2,306,691 - Pf6N2E2_1945 0.13 +0.4 2,306,691 - Pf6N2E2_1945 0.13 +0.2 2,306,691 - Pf6N2E2_1945 0.13 +1.1 2,306,691 - Pf6N2E2_1945 0.13 -0.1 2,306,691 - Pf6N2E2_1945 0.13 -0.3 2,306,691 - Pf6N2E2_1945 0.13 -0.1 2,306,748 - Pf6N2E2_1945 0.20 +1.0 2,306,941 - Pf6N2E2_1945 0.46 +1.6 2,306,972 + -0.9 2,307,113 + Pf6N2E2_1945 0.69 +1.9 2,307,114 - Pf6N2E2_1945 0.69 +1.2 2,307,114 - Pf6N2E2_1945 0.69 -0.4 2,307,114 - Pf6N2E2_1945 0.69 +0.8 2,307,114 - Pf6N2E2_1945 0.69 +1.6 2,307,114 - Pf6N2E2_1945 0.69 +0.5 2,307,114 - Pf6N2E2_1945 0.69 -1.1 2,307,169 + Pf6N2E2_1945 0.76 +0.1 2,307,182 + Pf6N2E2_1945 0.78 -0.2 2,307,183 - Pf6N2E2_1945 0.78 -0.7 2,307,183 - Pf6N2E2_1945 0.78 -0.3 2,307,183 - Pf6N2E2_1945 0.78 +0.7 2,307,183 - Pf6N2E2_1945 0.78 -1.2 2,307,183 - Pf6N2E2_1945 0.78 +0.1 2,307,296 + +0.5 2,307,337 + +0.2 2,307,337 + -1.5 2,307,338 - +0.6 2,307,338 - +0.9 2,307,343 - -0.4 2,307,343 - +0.3
Or see this region's nucleotide sequence