Experiment: D-Glucosamine Hydrochloride (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1662 and Pf6N2E2_1663 are separated by 31 nucleotides Pf6N2E2_1663 and Pf6N2E2_1664 overlap by 4 nucleotides
Pf6N2E2_1662 - 5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase., at 1,968,970 to 1,969,818
_1662
Pf6N2E2_1663 - 2-keto-3-deoxy-L-fuconate dehydrogenase, at 1,969,850 to 1,970,590
_1663
Pf6N2E2_1664 - Nitrate/nitrite transporter, at 1,970,587 to 1,971,969
_1664
Position (kb)
1969
1970
1971 Strain fitness (log2 ratio)
-1
0
1 at 1969.222 kb on + strand, within Pf6N2E2_1662 at 1969.223 kb on - strand, within Pf6N2E2_1662 at 1969.223 kb on - strand, within Pf6N2E2_1662 at 1969.223 kb on - strand, within Pf6N2E2_1662 at 1969.409 kb on + strand, within Pf6N2E2_1662 at 1969.410 kb on - strand, within Pf6N2E2_1662 at 1969.410 kb on - strand, within Pf6N2E2_1662 at 1969.521 kb on + strand, within Pf6N2E2_1662 at 1969.522 kb on - strand, within Pf6N2E2_1662 at 1969.810 kb on + strand at 1969.810 kb on + strand at 1969.855 kb on + strand at 1969.921 kb on - strand at 1969.921 kb on - strand at 1969.993 kb on - strand, within Pf6N2E2_1663 at 1970.274 kb on + strand, within Pf6N2E2_1663 at 1970.274 kb on + strand, within Pf6N2E2_1663 at 1970.333 kb on + strand, within Pf6N2E2_1663 at 1970.333 kb on + strand, within Pf6N2E2_1663 at 1970.333 kb on + strand, within Pf6N2E2_1663 at 1970.334 kb on - strand, within Pf6N2E2_1663 at 1970.334 kb on - strand, within Pf6N2E2_1663 at 1970.334 kb on - strand, within Pf6N2E2_1663 at 1970.353 kb on - strand, within Pf6N2E2_1663 at 1970.353 kb on - strand, within Pf6N2E2_1663 at 1970.567 kb on + strand at 1970.582 kb on + strand at 1970.583 kb on - strand at 1970.699 kb on - strand at 1970.699 kb on - strand at 1970.699 kb on - strand at 1970.699 kb on - strand at 1970.702 kb on + strand at 1970.702 kb on + strand at 1970.702 kb on + strand at 1970.702 kb on + strand at 1970.702 kb on + strand at 1970.702 kb on + strand at 1970.702 kb on + strand at 1970.702 kb on + strand at 1970.702 kb on + strand at 1970.703 kb on - strand at 1970.703 kb on - strand at 1970.703 kb on - strand at 1971.137 kb on + strand, within Pf6N2E2_1664 at 1971.427 kb on + strand, within Pf6N2E2_1664 at 1971.509 kb on + strand, within Pf6N2E2_1664 at 1971.510 kb on - strand, within Pf6N2E2_1664 at 1971.518 kb on + strand, within Pf6N2E2_1664 at 1971.519 kb on - strand, within Pf6N2E2_1664 at 1971.519 kb on - strand, within Pf6N2E2_1664 at 1971.578 kb on + strand, within Pf6N2E2_1664 at 1971.578 kb on + strand, within Pf6N2E2_1664 at 1971.578 kb on + strand, within Pf6N2E2_1664 at 1971.578 kb on + strand, within Pf6N2E2_1664 at 1971.579 kb on - strand, within Pf6N2E2_1664 at 1971.579 kb on - strand, within Pf6N2E2_1664 at 1971.579 kb on - strand, within Pf6N2E2_1664
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (C) remove 1,969,222 + Pf6N2E2_1662 0.30 +0.2 1,969,223 - Pf6N2E2_1662 0.30 +0.5 1,969,223 - Pf6N2E2_1662 0.30 +0.5 1,969,223 - Pf6N2E2_1662 0.30 +0.1 1,969,409 + Pf6N2E2_1662 0.52 -0.1 1,969,410 - Pf6N2E2_1662 0.52 +0.2 1,969,410 - Pf6N2E2_1662 0.52 +1.0 1,969,521 + Pf6N2E2_1662 0.65 -0.4 1,969,522 - Pf6N2E2_1662 0.65 -0.8 1,969,810 + -0.0 1,969,810 + +0.0 1,969,855 + +0.3 1,969,921 - +0.8 1,969,921 - +0.4 1,969,993 - Pf6N2E2_1663 0.19 -0.4 1,970,274 + Pf6N2E2_1663 0.57 -1.3 1,970,274 + Pf6N2E2_1663 0.57 -0.1 1,970,333 + Pf6N2E2_1663 0.65 -1.4 1,970,333 + Pf6N2E2_1663 0.65 +0.0 1,970,333 + Pf6N2E2_1663 0.65 +0.1 1,970,334 - Pf6N2E2_1663 0.65 -0.3 1,970,334 - Pf6N2E2_1663 0.65 -0.3 1,970,334 - Pf6N2E2_1663 0.65 -1.2 1,970,353 - Pf6N2E2_1663 0.68 -0.2 1,970,353 - Pf6N2E2_1663 0.68 -0.1 1,970,567 + -0.6 1,970,582 + -0.4 1,970,583 - +0.4 1,970,699 - +0.7 1,970,699 - -1.4 1,970,699 - -1.5 1,970,699 - +0.5 1,970,702 + +0.4 1,970,702 + -0.2 1,970,702 + +0.4 1,970,702 + -0.3 1,970,702 + -0.6 1,970,702 + +0.7 1,970,702 + +0.1 1,970,702 + -0.3 1,970,702 + +0.1 1,970,703 - +0.6 1,970,703 - +0.9 1,970,703 - -0.5 1,971,137 + Pf6N2E2_1664 0.40 +0.5 1,971,427 + Pf6N2E2_1664 0.61 -1.5 1,971,509 + Pf6N2E2_1664 0.67 +1.0 1,971,510 - Pf6N2E2_1664 0.67 +0.3 1,971,518 + Pf6N2E2_1664 0.67 +0.5 1,971,519 - Pf6N2E2_1664 0.67 +0.0 1,971,519 - Pf6N2E2_1664 0.67 +0.1 1,971,578 + Pf6N2E2_1664 0.72 +1.2 1,971,578 + Pf6N2E2_1664 0.72 +1.2 1,971,578 + Pf6N2E2_1664 0.72 -1.7 1,971,578 + Pf6N2E2_1664 0.72 -1.0 1,971,579 - Pf6N2E2_1664 0.72 -1.1 1,971,579 - Pf6N2E2_1664 0.72 +0.5 1,971,579 - Pf6N2E2_1664 0.72 +0.7
Or see this region's nucleotide sequence