Experiment: D-Glucosamine Hydrochloride (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1321 and Pf6N2E2_1322 overlap by 8 nucleotides Pf6N2E2_1322 and Pf6N2E2_1323 overlap by 8 nucleotides Pf6N2E2_1323 and Pf6N2E2_1324 are separated by 171 nucleotides
Pf6N2E2_1321 - MoaF protein precursor, at 1,554,840 to 1,555,661
_1321
Pf6N2E2_1322 - MoaE protein, at 1,555,654 to 1,556,454
_1322
Pf6N2E2_1323 - 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100), at 1,556,447 to 1,557,214
_1323
Pf6N2E2_1324 - Transcriptional regulator, AraC family, at 1,557,386 to 1,558,348
_1324
Position (kb) 1556
1557
1558 Strain fitness (log2 ratio) -1
0
1
2 at 1555.459 kb on - strand, within Pf6N2E2_1321 at 1555.459 kb on - strand, within Pf6N2E2_1321 at 1555.480 kb on - strand, within Pf6N2E2_1321 at 1555.480 kb on - strand, within Pf6N2E2_1321 at 1555.630 kb on + strand at 1555.648 kb on - strand at 1555.669 kb on - strand at 1555.723 kb on + strand at 1555.911 kb on + strand, within Pf6N2E2_1322 at 1555.990 kb on + strand, within Pf6N2E2_1322 at 1555.991 kb on - strand, within Pf6N2E2_1322 at 1555.991 kb on - strand, within Pf6N2E2_1322 at 1556.263 kb on + strand, within Pf6N2E2_1322 at 1556.263 kb on + strand, within Pf6N2E2_1322 at 1556.264 kb on - strand, within Pf6N2E2_1322 at 1556.264 kb on - strand, within Pf6N2E2_1322 at 1556.412 kb on - strand at 1556.412 kb on - strand at 1556.625 kb on + strand, within Pf6N2E2_1323 at 1556.625 kb on + strand, within Pf6N2E2_1323 at 1556.783 kb on - strand, within Pf6N2E2_1323 at 1557.134 kb on + strand, within Pf6N2E2_1323 at 1557.158 kb on + strand at 1557.158 kb on + strand at 1557.159 kb on - strand at 1557.159 kb on - strand at 1557.515 kb on - strand, within Pf6N2E2_1324 at 1557.515 kb on - strand, within Pf6N2E2_1324 at 1557.584 kb on - strand, within Pf6N2E2_1324 at 1557.586 kb on - strand, within Pf6N2E2_1324 at 1557.713 kb on + strand, within Pf6N2E2_1324 at 1557.713 kb on + strand, within Pf6N2E2_1324 at 1557.713 kb on + strand, within Pf6N2E2_1324 at 1557.713 kb on + strand, within Pf6N2E2_1324 at 1557.714 kb on - strand, within Pf6N2E2_1324 at 1557.714 kb on - strand, within Pf6N2E2_1324 at 1557.714 kb on - strand, within Pf6N2E2_1324 at 1557.714 kb on - strand, within Pf6N2E2_1324 at 1557.717 kb on + strand, within Pf6N2E2_1324 at 1557.718 kb on - strand, within Pf6N2E2_1324 at 1557.718 kb on - strand, within Pf6N2E2_1324 at 1557.718 kb on - strand, within Pf6N2E2_1324 at 1557.718 kb on - strand, within Pf6N2E2_1324 at 1557.718 kb on - strand, within Pf6N2E2_1324 at 1557.770 kb on - strand, within Pf6N2E2_1324 at 1558.098 kb on + strand, within Pf6N2E2_1324 at 1558.157 kb on + strand, within Pf6N2E2_1324
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (C) remove 1,555,459 - Pf6N2E2_1321 0.75 +0.1 1,555,459 - Pf6N2E2_1321 0.75 +0.0 1,555,480 - Pf6N2E2_1321 0.78 +1.9 1,555,480 - Pf6N2E2_1321 0.78 -0.3 1,555,630 + +0.5 1,555,648 - +1.0 1,555,669 - -0.6 1,555,723 + +1.4 1,555,911 + Pf6N2E2_1322 0.32 -0.7 1,555,990 + Pf6N2E2_1322 0.42 -0.6 1,555,991 - Pf6N2E2_1322 0.42 -0.6 1,555,991 - Pf6N2E2_1322 0.42 +1.4 1,556,263 + Pf6N2E2_1322 0.76 -0.2 1,556,263 + Pf6N2E2_1322 0.76 +0.4 1,556,264 - Pf6N2E2_1322 0.76 +0.1 1,556,264 - Pf6N2E2_1322 0.76 +1.1 1,556,412 - -1.2 1,556,412 - +0.3 1,556,625 + Pf6N2E2_1323 0.23 -1.3 1,556,625 + Pf6N2E2_1323 0.23 -1.0 1,556,783 - Pf6N2E2_1323 0.44 -1.3 1,557,134 + Pf6N2E2_1323 0.89 +0.2 1,557,158 + +0.3 1,557,158 + -0.8 1,557,159 - +0.8 1,557,159 - +0.3 1,557,515 - Pf6N2E2_1324 0.13 -0.9 1,557,515 - Pf6N2E2_1324 0.13 +0.5 1,557,584 - Pf6N2E2_1324 0.21 -0.1 1,557,586 - Pf6N2E2_1324 0.21 +0.2 1,557,713 + Pf6N2E2_1324 0.34 -0.1 1,557,713 + Pf6N2E2_1324 0.34 +0.8 1,557,713 + Pf6N2E2_1324 0.34 +0.0 1,557,713 + Pf6N2E2_1324 0.34 -0.3 1,557,714 - Pf6N2E2_1324 0.34 +1.0 1,557,714 - Pf6N2E2_1324 0.34 +0.6 1,557,714 - Pf6N2E2_1324 0.34 -0.5 1,557,714 - Pf6N2E2_1324 0.34 +0.3 1,557,717 + Pf6N2E2_1324 0.34 -0.4 1,557,718 - Pf6N2E2_1324 0.34 -0.2 1,557,718 - Pf6N2E2_1324 0.34 +0.5 1,557,718 - Pf6N2E2_1324 0.34 -0.3 1,557,718 - Pf6N2E2_1324 0.34 +0.7 1,557,718 - Pf6N2E2_1324 0.34 +0.9 1,557,770 - Pf6N2E2_1324 0.40 -0.4 1,558,098 + Pf6N2E2_1324 0.74 +0.6 1,558,157 + Pf6N2E2_1324 0.80 +0.9
Or see this region's nucleotide sequence