Experiment: D-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_845 and Pf1N1B4_846 are separated by 232 nucleotides Pf1N1B4_846 and Pf1N1B4_847 are separated by 15 nucleotides Pf1N1B4_847 and Pf1N1B4_848 are separated by 164 nucleotides Pf1N1B4_848 and Pf1N1B4_849 overlap by 13 nucleotides
Pf1N1B4_845 - Membrane proteins related to metalloendopeptidases, at 973,712 to 974,533
_845
Pf1N1B4_846 - hypothetical protein, at 974,766 to 974,969
_846
Pf1N1B4_847 - 2-isopropylmalate synthase (EC 2.3.3.13), at 974,985 to 976,664
_847
Pf1N1B4_848 - Aliphatic amidase AmiE (EC 3.5.1.4), at 976,829 to 977,620
_848
Pf1N1B4_849 - Aspartate aminotransferase (EC 2.6.1.1), at 977,608 to 978,756
_849
Position (kb)
974
975
976
977 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 974.003 kb on + strand, within Pf1N1B4_845 at 974.215 kb on + strand, within Pf1N1B4_845 at 974.215 kb on + strand, within Pf1N1B4_845 at 974.215 kb on + strand, within Pf1N1B4_845 at 974.216 kb on - strand, within Pf1N1B4_845 at 974.216 kb on - strand, within Pf1N1B4_845 at 974.216 kb on - strand, within Pf1N1B4_845 at 974.216 kb on - strand, within Pf1N1B4_845 at 974.216 kb on - strand, within Pf1N1B4_845 at 974.216 kb on - strand, within Pf1N1B4_845 at 974.304 kb on - strand, within Pf1N1B4_845 at 974.304 kb on - strand, within Pf1N1B4_845 at 974.304 kb on - strand, within Pf1N1B4_845 at 974.368 kb on + strand, within Pf1N1B4_845 at 974.368 kb on + strand, within Pf1N1B4_845 at 974.368 kb on + strand, within Pf1N1B4_845 at 974.369 kb on - strand, within Pf1N1B4_845 at 974.369 kb on - strand, within Pf1N1B4_845 at 974.461 kb on + strand at 974.461 kb on + strand at 974.462 kb on - strand at 974.462 kb on - strand at 974.656 kb on - strand at 974.656 kb on - strand at 974.729 kb on + strand at 974.915 kb on + strand, within Pf1N1B4_846 at 974.915 kb on + strand at 974.916 kb on - strand at 974.922 kb on + strand, within Pf1N1B4_846 at 974.972 kb on + strand at 974.972 kb on + strand at 975.016 kb on - strand at 975.016 kb on - strand at 975.246 kb on - strand, within Pf1N1B4_847 at 975.246 kb on - strand, within Pf1N1B4_847 at 975.326 kb on + strand, within Pf1N1B4_847 at 975.327 kb on - strand, within Pf1N1B4_847 at 975.327 kb on - strand at 975.327 kb on - strand, within Pf1N1B4_847 at 975.372 kb on + strand, within Pf1N1B4_847 at 975.372 kb on + strand, within Pf1N1B4_847 at 975.373 kb on - strand, within Pf1N1B4_847 at 975.373 kb on - strand, within Pf1N1B4_847 at 975.373 kb on - strand at 975.654 kb on + strand, within Pf1N1B4_847 at 975.654 kb on + strand, within Pf1N1B4_847 at 975.654 kb on + strand, within Pf1N1B4_847 at 975.654 kb on + strand, within Pf1N1B4_847 at 975.655 kb on - strand, within Pf1N1B4_847 at 975.655 kb on - strand, within Pf1N1B4_847 at 975.655 kb on - strand, within Pf1N1B4_847 at 975.655 kb on - strand, within Pf1N1B4_847 at 975.655 kb on - strand, within Pf1N1B4_847 at 975.655 kb on - strand at 975.861 kb on + strand, within Pf1N1B4_847 at 975.861 kb on + strand, within Pf1N1B4_847 at 975.862 kb on - strand, within Pf1N1B4_847 at 975.862 kb on - strand, within Pf1N1B4_847 at 975.862 kb on - strand at 975.862 kb on - strand, within Pf1N1B4_847 at 975.963 kb on + strand, within Pf1N1B4_847 at 976.065 kb on + strand, within Pf1N1B4_847 at 976.065 kb on + strand, within Pf1N1B4_847 at 976.065 kb on + strand, within Pf1N1B4_847 at 976.066 kb on - strand, within Pf1N1B4_847 at 976.066 kb on - strand, within Pf1N1B4_847 at 976.101 kb on + strand, within Pf1N1B4_847 at 976.101 kb on + strand, within Pf1N1B4_847 at 976.102 kb on - strand, within Pf1N1B4_847 at 976.152 kb on + strand at 976.152 kb on + strand, within Pf1N1B4_847 at 976.153 kb on - strand, within Pf1N1B4_847 at 976.170 kb on + strand, within Pf1N1B4_847 at 976.290 kb on + strand, within Pf1N1B4_847 at 976.290 kb on + strand, within Pf1N1B4_847 at 976.291 kb on - strand, within Pf1N1B4_847 at 976.479 kb on + strand, within Pf1N1B4_847 at 976.479 kb on + strand, within Pf1N1B4_847 at 976.479 kb on + strand, within Pf1N1B4_847 at 976.480 kb on - strand, within Pf1N1B4_847 at 976.527 kb on + strand at 976.527 kb on + strand at 976.527 kb on + strand at 976.528 kb on - strand at 976.528 kb on - strand at 976.528 kb on - strand at 976.528 kb on - strand at 976.528 kb on - strand at 976.543 kb on + strand at 976.988 kb on - strand, within Pf1N1B4_848 at 976.989 kb on + strand, within Pf1N1B4_848 at 976.990 kb on - strand, within Pf1N1B4_848 at 976.990 kb on - strand, within Pf1N1B4_848 at 976.990 kb on - strand, within Pf1N1B4_848 at 976.990 kb on - strand, within Pf1N1B4_848 at 977.037 kb on + strand, within Pf1N1B4_848 at 977.037 kb on + strand at 977.038 kb on - strand, within Pf1N1B4_848 at 977.038 kb on - strand, within Pf1N1B4_848 at 977.038 kb on - strand, within Pf1N1B4_848 at 977.038 kb on - strand, within Pf1N1B4_848 at 977.038 kb on - strand, within Pf1N1B4_848 at 977.116 kb on - strand, within Pf1N1B4_848 at 977.173 kb on - strand, within Pf1N1B4_848 at 977.283 kb on + strand, within Pf1N1B4_848 at 977.284 kb on - strand, within Pf1N1B4_848 at 977.410 kb on + strand, within Pf1N1B4_848 at 977.410 kb on + strand, within Pf1N1B4_848 at 977.411 kb on - strand, within Pf1N1B4_848 at 977.620 kb on + strand at 977.621 kb on - strand at 977.621 kb on - strand at 977.621 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Lactate (C) remove 974,003 + Pf1N1B4_845 0.35 +0.6 974,215 + Pf1N1B4_845 0.61 +0.5 974,215 + Pf1N1B4_845 0.61 -0.3 974,215 + Pf1N1B4_845 0.61 -1.0 974,216 - Pf1N1B4_845 0.61 -0.3 974,216 - Pf1N1B4_845 0.61 +1.3 974,216 - Pf1N1B4_845 0.61 +0.0 974,216 - Pf1N1B4_845 0.61 -0.3 974,216 - Pf1N1B4_845 0.61 -0.3 974,216 - Pf1N1B4_845 0.61 +0.7 974,304 - Pf1N1B4_845 0.72 -0.3 974,304 - Pf1N1B4_845 0.72 -1.0 974,304 - Pf1N1B4_845 0.72 +0.5 974,368 + Pf1N1B4_845 0.80 -0.7 974,368 + Pf1N1B4_845 0.80 +0.5 974,368 + Pf1N1B4_845 0.80 +0.0 974,369 - Pf1N1B4_845 0.80 -0.7 974,369 - Pf1N1B4_845 0.80 +0.3 974,461 + -0.9 974,461 + +0.3 974,462 - -1.4 974,462 - +0.3 974,656 - -0.8 974,656 - -1.0 974,729 + +1.1 974,915 + Pf1N1B4_846 0.73 -0.3 974,915 + +0.7 974,916 - +0.3 974,922 + Pf1N1B4_846 0.76 +0.4 974,972 + -0.3 974,972 + -1.9 975,016 - -1.0 975,016 - -1.5 975,246 - Pf1N1B4_847 0.16 -2.3 975,246 - Pf1N1B4_847 0.16 -3.3 975,326 + Pf1N1B4_847 0.20 -1.7 975,327 - Pf1N1B4_847 0.20 -2.3 975,327 - +1.3 975,327 - Pf1N1B4_847 0.20 -1.0 975,372 + Pf1N1B4_847 0.23 -1.0 975,372 + Pf1N1B4_847 0.23 -0.6 975,373 - Pf1N1B4_847 0.23 -1.7 975,373 - Pf1N1B4_847 0.23 -1.0 975,373 - +0.3 975,654 + Pf1N1B4_847 0.40 -0.3 975,654 + Pf1N1B4_847 0.40 -2.5 975,654 + Pf1N1B4_847 0.40 -2.2 975,654 + Pf1N1B4_847 0.40 -1.3 975,655 - Pf1N1B4_847 0.40 -4.5 975,655 - Pf1N1B4_847 0.40 +0.3 975,655 - Pf1N1B4_847 0.40 -2.6 975,655 - Pf1N1B4_847 0.40 -1.9 975,655 - Pf1N1B4_847 0.40 -3.4 975,655 - -0.7 975,861 + Pf1N1B4_847 0.52 -2.5 975,861 + Pf1N1B4_847 0.52 -3.0 975,862 - Pf1N1B4_847 0.52 -0.7 975,862 - Pf1N1B4_847 0.52 -1.7 975,862 - -1.3 975,862 - Pf1N1B4_847 0.52 -0.7 975,963 + Pf1N1B4_847 0.58 -1.9 976,065 + Pf1N1B4_847 0.64 -1.0 976,065 + Pf1N1B4_847 0.64 -2.2 976,065 + Pf1N1B4_847 0.64 -4.3 976,066 - Pf1N1B4_847 0.64 -0.7 976,066 - Pf1N1B4_847 0.64 -1.0 976,101 + Pf1N1B4_847 0.66 -2.3 976,101 + Pf1N1B4_847 0.66 -1.3 976,102 - Pf1N1B4_847 0.66 +0.3 976,152 + +0.3 976,152 + Pf1N1B4_847 0.69 +1.3 976,153 - Pf1N1B4_847 0.70 -1.7 976,170 + Pf1N1B4_847 0.71 -2.2 976,290 + Pf1N1B4_847 0.78 -3.2 976,290 + Pf1N1B4_847 0.78 -2.3 976,291 - Pf1N1B4_847 0.78 -4.1 976,479 + Pf1N1B4_847 0.89 +0.5 976,479 + Pf1N1B4_847 0.89 -0.9 976,479 + Pf1N1B4_847 0.89 -0.2 976,480 - Pf1N1B4_847 0.89 +0.8 976,527 + -0.7 976,527 + -1.0 976,527 + -1.9 976,528 - -0.3 976,528 - -0.5 976,528 - -1.9 976,528 - -1.7 976,528 - -1.7 976,543 + -0.3 976,988 - Pf1N1B4_848 0.20 +0.4 976,989 + Pf1N1B4_848 0.20 +0.9 976,990 - Pf1N1B4_848 0.20 -0.5 976,990 - Pf1N1B4_848 0.20 -0.0 976,990 - Pf1N1B4_848 0.20 -1.2 976,990 - Pf1N1B4_848 0.20 +1.3 977,037 + Pf1N1B4_848 0.26 -0.2 977,037 + +0.3 977,038 - Pf1N1B4_848 0.26 +2.6 977,038 - Pf1N1B4_848 0.26 -0.1 977,038 - Pf1N1B4_848 0.26 +0.6 977,038 - Pf1N1B4_848 0.26 +0.7 977,038 - Pf1N1B4_848 0.26 +0.5 977,116 - Pf1N1B4_848 0.36 +0.5 977,173 - Pf1N1B4_848 0.43 +0.1 977,283 + Pf1N1B4_848 0.57 +0.1 977,284 - Pf1N1B4_848 0.57 -1.9 977,410 + Pf1N1B4_848 0.73 -1.0 977,410 + Pf1N1B4_848 0.73 +0.1 977,411 - Pf1N1B4_848 0.73 -1.5 977,620 + +1.3 977,621 - -0.5 977,621 - +0.2 977,621 - +0.3
Or see this region's nucleotide sequence