Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_825

Experiment: D-Lactate (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_823 and Pf1N1B4_824 are separated by 2 nucleotidesPf1N1B4_824 and Pf1N1B4_825 are separated by 150 nucleotidesPf1N1B4_825 and Pf1N1B4_826 overlap by 4 nucleotides Pf1N1B4_823 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 945,479 to 945,871 _823 Pf1N1B4_824 - POSSIBLE MEMBRANE PROTEIN, at 945,874 to 946,518 _824 Pf1N1B4_825 - tRNA (uridine-5-oxyacetic acid methyl ester) 34 synthase, at 946,669 to 947,412 _825 Pf1N1B4_826 - tRNA (5-methoxyuridine) 34 synthase, at 947,409 to 948,365 _826 Position (kb) 946 947 948Strain fitness (log2 ratio) -2 -1 0 1 2at 945.704 kb on + strand, within Pf1N1B4_823at 945.803 kb on + strand, within Pf1N1B4_823at 945.803 kb on + strand, within Pf1N1B4_823at 945.803 kb on + strand, within Pf1N1B4_823at 945.804 kb on - strand, within Pf1N1B4_823at 946.135 kb on + strand, within Pf1N1B4_824at 946.135 kb on + strand, within Pf1N1B4_824at 946.412 kb on + strand, within Pf1N1B4_824at 946.413 kb on - strand, within Pf1N1B4_824at 946.413 kb on - strand, within Pf1N1B4_824at 946.435 kb on + strand, within Pf1N1B4_824at 946.435 kb on + strand, within Pf1N1B4_824at 946.435 kb on + strand, within Pf1N1B4_824at 946.436 kb on - strand, within Pf1N1B4_824at 946.436 kb on - strand, within Pf1N1B4_824at 946.436 kb on - strand, within Pf1N1B4_824at 946.453 kb on + strand, within Pf1N1B4_824at 946.453 kb on + strand, within Pf1N1B4_824at 946.454 kb on - strandat 946.852 kb on + strand, within Pf1N1B4_825at 946.852 kb on + strand, within Pf1N1B4_825at 946.938 kb on + strand, within Pf1N1B4_825at 946.972 kb on + strand, within Pf1N1B4_825at 946.973 kb on - strand, within Pf1N1B4_825at 947.251 kb on + strand, within Pf1N1B4_825at 947.383 kb on - strandat 947.799 kb on + strand, within Pf1N1B4_826at 947.802 kb on + strandat 947.802 kb on + strand, within Pf1N1B4_826at 947.802 kb on + strand, within Pf1N1B4_826at 947.802 kb on + strand, within Pf1N1B4_826at 947.802 kb on + strand, within Pf1N1B4_826at 947.802 kb on + strand, within Pf1N1B4_826at 947.803 kb on - strand, within Pf1N1B4_826at 947.803 kb on - strand, within Pf1N1B4_826at 947.803 kb on - strand, within Pf1N1B4_826at 947.803 kb on - strand, within Pf1N1B4_826at 947.803 kb on - strand, within Pf1N1B4_826at 947.803 kb on - strand, within Pf1N1B4_826at 947.895 kb on + strand, within Pf1N1B4_826at 947.895 kb on + strandat 947.896 kb on - strand, within Pf1N1B4_826at 947.995 kb on - strand, within Pf1N1B4_826at 948.267 kb on + strandat 948.267 kb on + strand, within Pf1N1B4_826at 948.267 kb on + strand, within Pf1N1B4_826at 948.267 kb on + strand, within Pf1N1B4_826at 948.267 kb on + strand, within Pf1N1B4_826at 948.268 kb on - strand, within Pf1N1B4_826at 948.363 kb on + strandat 948.363 kb on + strandat 948.363 kb on + strandat 948.388 kb on + strandat 948.388 kb on + strandat 948.389 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Lactate (C)
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945,704 + Pf1N1B4_823 0.57 -0.7
945,803 + Pf1N1B4_823 0.82 -0.8
945,803 + Pf1N1B4_823 0.82 +0.3
945,803 + Pf1N1B4_823 0.82 +0.8
945,804 - Pf1N1B4_823 0.83 +0.9
946,135 + Pf1N1B4_824 0.40 +0.3
946,135 + Pf1N1B4_824 0.40 -1.2
946,412 + Pf1N1B4_824 0.83 -0.7
946,413 - Pf1N1B4_824 0.84 -0.2
946,413 - Pf1N1B4_824 0.84 +0.1
946,435 + Pf1N1B4_824 0.87 +0.5
946,435 + Pf1N1B4_824 0.87 +1.3
946,435 + Pf1N1B4_824 0.87 -0.1
946,436 - Pf1N1B4_824 0.87 -0.3
946,436 - Pf1N1B4_824 0.87 +0.3
946,436 - Pf1N1B4_824 0.87 +1.6
946,453 + Pf1N1B4_824 0.90 -0.3
946,453 + Pf1N1B4_824 0.90 +1.6
946,454 - +0.9
946,852 + Pf1N1B4_825 0.25 -0.1
946,852 + Pf1N1B4_825 0.25 -2.5
946,938 + Pf1N1B4_825 0.36 +1.3
946,972 + Pf1N1B4_825 0.41 +1.0
946,973 - Pf1N1B4_825 0.41 +0.3
947,251 + Pf1N1B4_825 0.78 +1.9
947,383 - +0.7
947,799 + Pf1N1B4_826 0.41 -0.3
947,802 + +0.7
947,802 + Pf1N1B4_826 0.41 -0.3
947,802 + Pf1N1B4_826 0.41 -0.5
947,802 + Pf1N1B4_826 0.41 -0.1
947,802 + Pf1N1B4_826 0.41 +0.7
947,802 + Pf1N1B4_826 0.41 -0.3
947,803 - Pf1N1B4_826 0.41 -1.9
947,803 - Pf1N1B4_826 0.41 -1.4
947,803 - Pf1N1B4_826 0.41 +1.3
947,803 - Pf1N1B4_826 0.41 +0.3
947,803 - Pf1N1B4_826 0.41 -1.9
947,803 - Pf1N1B4_826 0.41 +1.3
947,895 + Pf1N1B4_826 0.51 -1.0
947,895 + +2.3
947,896 - Pf1N1B4_826 0.51 -0.8
947,995 - Pf1N1B4_826 0.61 +0.3
948,267 + -0.7
948,267 + Pf1N1B4_826 0.90 +0.7
948,267 + Pf1N1B4_826 0.90 -0.3
948,267 + Pf1N1B4_826 0.90 +0.1
948,267 + Pf1N1B4_826 0.90 +0.7
948,268 - Pf1N1B4_826 0.90 -1.1
948,363 + -0.3
948,363 + -0.7
948,363 + -0.1
948,388 + +0.9
948,388 + +2.0
948,389 - +0.3

Or see this region's nucleotide sequence