Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5026

Experiment: D-Lactate (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5024 and Pf1N1B4_5025 overlap by 37 nucleotidesPf1N1B4_5025 and Pf1N1B4_5026 are separated by 53 nucleotidesPf1N1B4_5026 and Pf1N1B4_5027 are separated by 160 nucleotidesPf1N1B4_5027 and Pf1N1B4_5028 overlap by 22 nucleotides Pf1N1B4_5024 - ADA regulatory protein / Methylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.63), at 5,571,445 to 5,572,545 _5024 Pf1N1B4_5025 - Probable transcription regulator, at 5,572,509 to 5,572,997 _5025 Pf1N1B4_5026 - Alcohol dehydrogenase (EC 1.1.1.1), at 5,573,051 to 5,574,034 _5026 Pf1N1B4_5027 - Enoyl-[acyl-carrier-protein] reductase [FMN] (EC 1.3.1.9), at 5,574,195 to 5,574,779 _5027 Pf1N1B4_5028 - Enoyl-[acyl-carrier-protein] reductase [FMN] (EC 1.3.1.9), at 5,574,758 to 5,575,258 _5028 Position (kb) 5573 5574 5575Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 5572.280 kb on + strand, within Pf1N1B4_5024at 5572.351 kb on + strand, within Pf1N1B4_5024at 5572.361 kb on + strand, within Pf1N1B4_5024at 5572.361 kb on + strand, within Pf1N1B4_5024at 5572.361 kb on + strand, within Pf1N1B4_5024at 5572.362 kb on - strand, within Pf1N1B4_5024at 5572.582 kb on + strand, within Pf1N1B4_5025at 5572.583 kb on - strand, within Pf1N1B4_5025at 5572.637 kb on - strand, within Pf1N1B4_5025at 5572.637 kb on - strand, within Pf1N1B4_5025at 5572.705 kb on + strand, within Pf1N1B4_5025at 5572.804 kb on + strandat 5572.805 kb on - strand, within Pf1N1B4_5025at 5572.805 kb on - strand, within Pf1N1B4_5025at 5572.814 kb on - strand, within Pf1N1B4_5025at 5572.822 kb on + strand, within Pf1N1B4_5025at 5572.892 kb on + strand, within Pf1N1B4_5025at 5572.893 kb on - strand, within Pf1N1B4_5025at 5572.910 kb on + strand, within Pf1N1B4_5025at 5572.989 kb on + strandat 5572.989 kb on + strandat 5572.989 kb on + strandat 5572.990 kb on - strandat 5572.990 kb on - strandat 5572.990 kb on - strandat 5573.018 kb on + strandat 5573.492 kb on - strand, within Pf1N1B4_5026at 5574.078 kb on + strandat 5574.151 kb on + strandat 5574.157 kb on + strandat 5574.483 kb on + strand, within Pf1N1B4_5027at 5574.484 kb on - strand, within Pf1N1B4_5027at 5574.789 kb on + strandat 5574.926 kb on + strand, within Pf1N1B4_5028at 5574.926 kb on + strand, within Pf1N1B4_5028at 5574.926 kb on + strand, within Pf1N1B4_5028at 5574.926 kb on + strand, within Pf1N1B4_5028at 5574.926 kb on + strand, within Pf1N1B4_5028at 5574.927 kb on - strand, within Pf1N1B4_5028

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Lactate (C)
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5,572,280 + Pf1N1B4_5024 0.76 -0.5
5,572,351 + Pf1N1B4_5024 0.82 -2.0
5,572,361 + Pf1N1B4_5024 0.83 +0.8
5,572,361 + Pf1N1B4_5024 0.83 -0.2
5,572,361 + Pf1N1B4_5024 0.83 -0.4
5,572,362 - Pf1N1B4_5024 0.83 -0.5
5,572,582 + Pf1N1B4_5025 0.15 +0.4
5,572,583 - Pf1N1B4_5025 0.15 +0.8
5,572,637 - Pf1N1B4_5025 0.26 -0.2
5,572,637 - Pf1N1B4_5025 0.26 +0.5
5,572,705 + Pf1N1B4_5025 0.40 +0.0
5,572,804 + +0.8
5,572,805 - Pf1N1B4_5025 0.61 -0.4
5,572,805 - Pf1N1B4_5025 0.61 +0.1
5,572,814 - Pf1N1B4_5025 0.62 -0.1
5,572,822 + Pf1N1B4_5025 0.64 -1.4
5,572,892 + Pf1N1B4_5025 0.78 -1.0
5,572,893 - Pf1N1B4_5025 0.79 -0.1
5,572,910 + Pf1N1B4_5025 0.82 +0.5
5,572,989 + -0.4
5,572,989 + -1.0
5,572,989 + +0.1
5,572,990 - -0.2
5,572,990 - +0.5
5,572,990 - +0.3
5,573,018 + +0.0
5,573,492 - Pf1N1B4_5026 0.45 +0.4
5,574,078 + +1.8
5,574,151 + -0.6
5,574,157 + -0.8
5,574,483 + Pf1N1B4_5027 0.49 +1.3
5,574,484 - Pf1N1B4_5027 0.49 +0.8
5,574,789 + -0.2
5,574,926 + Pf1N1B4_5028 0.34 +0.4
5,574,926 + Pf1N1B4_5028 0.34 +0.0
5,574,926 + Pf1N1B4_5028 0.34 +0.4
5,574,926 + Pf1N1B4_5028 0.34 +1.8
5,574,926 + Pf1N1B4_5028 0.34 -0.5
5,574,927 - Pf1N1B4_5028 0.34 -3.1

Or see this region's nucleotide sequence