Experiment: D-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5024 and Pf1N1B4_5025 overlap by 37 nucleotides Pf1N1B4_5025 and Pf1N1B4_5026 are separated by 53 nucleotides Pf1N1B4_5026 and Pf1N1B4_5027 are separated by 160 nucleotides Pf1N1B4_5027 and Pf1N1B4_5028 overlap by 22 nucleotides
Pf1N1B4_5024 - ADA regulatory protein / Methylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.63), at 5,571,445 to 5,572,545
_5024
Pf1N1B4_5025 - Probable transcription regulator, at 5,572,509 to 5,572,997
_5025
Pf1N1B4_5026 - Alcohol dehydrogenase (EC 1.1.1.1), at 5,573,051 to 5,574,034
_5026
Pf1N1B4_5027 - Enoyl-[acyl-carrier-protein] reductase [FMN] (EC 1.3.1.9), at 5,574,195 to 5,574,779
_5027
Pf1N1B4_5028 - Enoyl-[acyl-carrier-protein] reductase [FMN] (EC 1.3.1.9), at 5,574,758 to 5,575,258
_5028
Position (kb)
5573
5574
5575 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5572.280 kb on + strand, within Pf1N1B4_5024 at 5572.351 kb on + strand, within Pf1N1B4_5024 at 5572.361 kb on + strand, within Pf1N1B4_5024 at 5572.361 kb on + strand, within Pf1N1B4_5024 at 5572.361 kb on + strand, within Pf1N1B4_5024 at 5572.362 kb on - strand, within Pf1N1B4_5024 at 5572.582 kb on + strand, within Pf1N1B4_5025 at 5572.583 kb on - strand, within Pf1N1B4_5025 at 5572.637 kb on - strand, within Pf1N1B4_5025 at 5572.637 kb on - strand, within Pf1N1B4_5025 at 5572.705 kb on + strand, within Pf1N1B4_5025 at 5572.804 kb on + strand at 5572.805 kb on - strand, within Pf1N1B4_5025 at 5572.805 kb on - strand, within Pf1N1B4_5025 at 5572.814 kb on - strand, within Pf1N1B4_5025 at 5572.822 kb on + strand, within Pf1N1B4_5025 at 5572.892 kb on + strand, within Pf1N1B4_5025 at 5572.893 kb on - strand, within Pf1N1B4_5025 at 5572.910 kb on + strand, within Pf1N1B4_5025 at 5572.989 kb on + strand at 5572.989 kb on + strand at 5572.989 kb on + strand at 5572.990 kb on - strand at 5572.990 kb on - strand at 5572.990 kb on - strand at 5573.018 kb on + strand at 5573.492 kb on - strand, within Pf1N1B4_5026 at 5574.078 kb on + strand at 5574.151 kb on + strand at 5574.157 kb on + strand at 5574.483 kb on + strand, within Pf1N1B4_5027 at 5574.484 kb on - strand, within Pf1N1B4_5027 at 5574.789 kb on + strand at 5574.926 kb on + strand, within Pf1N1B4_5028 at 5574.926 kb on + strand, within Pf1N1B4_5028 at 5574.926 kb on + strand, within Pf1N1B4_5028 at 5574.926 kb on + strand, within Pf1N1B4_5028 at 5574.926 kb on + strand, within Pf1N1B4_5028 at 5574.927 kb on - strand, within Pf1N1B4_5028
Per-strain Table
Position Strand Gene LocusTag Fraction D-Lactate (C) remove 5,572,280 + Pf1N1B4_5024 0.76 -0.5 5,572,351 + Pf1N1B4_5024 0.82 -2.0 5,572,361 + Pf1N1B4_5024 0.83 +0.8 5,572,361 + Pf1N1B4_5024 0.83 -0.2 5,572,361 + Pf1N1B4_5024 0.83 -0.4 5,572,362 - Pf1N1B4_5024 0.83 -0.5 5,572,582 + Pf1N1B4_5025 0.15 +0.4 5,572,583 - Pf1N1B4_5025 0.15 +0.8 5,572,637 - Pf1N1B4_5025 0.26 -0.2 5,572,637 - Pf1N1B4_5025 0.26 +0.5 5,572,705 + Pf1N1B4_5025 0.40 +0.0 5,572,804 + +0.8 5,572,805 - Pf1N1B4_5025 0.61 -0.4 5,572,805 - Pf1N1B4_5025 0.61 +0.1 5,572,814 - Pf1N1B4_5025 0.62 -0.1 5,572,822 + Pf1N1B4_5025 0.64 -1.4 5,572,892 + Pf1N1B4_5025 0.78 -1.0 5,572,893 - Pf1N1B4_5025 0.79 -0.1 5,572,910 + Pf1N1B4_5025 0.82 +0.5 5,572,989 + -0.4 5,572,989 + -1.0 5,572,989 + +0.1 5,572,990 - -0.2 5,572,990 - +0.5 5,572,990 - +0.3 5,573,018 + +0.0 5,573,492 - Pf1N1B4_5026 0.45 +0.4 5,574,078 + +1.8 5,574,151 + -0.6 5,574,157 + -0.8 5,574,483 + Pf1N1B4_5027 0.49 +1.3 5,574,484 - Pf1N1B4_5027 0.49 +0.8 5,574,789 + -0.2 5,574,926 + Pf1N1B4_5028 0.34 +0.4 5,574,926 + Pf1N1B4_5028 0.34 +0.0 5,574,926 + Pf1N1B4_5028 0.34 +0.4 5,574,926 + Pf1N1B4_5028 0.34 +1.8 5,574,926 + Pf1N1B4_5028 0.34 -0.5 5,574,927 - Pf1N1B4_5028 0.34 -3.1
Or see this region's nucleotide sequence