Experiment: D-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4686 and Pf1N1B4_4687 are separated by 55 nucleotides Pf1N1B4_4687 and Pf1N1B4_4688 are separated by 600 nucleotides
Pf1N1B4_4686 - Malto-oligosyltrehalose trehalohydrolase (EC 3.2.1.141), at 5,176,537 to 5,178,291
_4686
Pf1N1B4_4687 - Glycogen synthase, ADP-glucose transglucosylase (EC 2.4.1.21), at 5,178,347 to 5,179,933
_4687
Pf1N1B4_4688 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 5,180,534 to 5,181,487
_4688
Position (kb)
5178
5179
5180 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5177.351 kb on + strand, within Pf1N1B4_4686 at 5177.351 kb on + strand, within Pf1N1B4_4686 at 5177.739 kb on - strand, within Pf1N1B4_4686 at 5177.862 kb on + strand, within Pf1N1B4_4686 at 5177.984 kb on + strand, within Pf1N1B4_4686 at 5177.984 kb on + strand at 5178.115 kb on - strand, within Pf1N1B4_4686 at 5178.115 kb on - strand, within Pf1N1B4_4686 at 5178.197 kb on - strand at 5178.348 kb on + strand at 5178.348 kb on + strand at 5178.349 kb on - strand at 5178.393 kb on + strand at 5178.631 kb on - strand, within Pf1N1B4_4687 at 5178.837 kb on + strand, within Pf1N1B4_4687 at 5178.837 kb on + strand at 5178.837 kb on + strand, within Pf1N1B4_4687 at 5178.838 kb on - strand, within Pf1N1B4_4687 at 5179.134 kb on + strand, within Pf1N1B4_4687 at 5179.134 kb on + strand at 5179.135 kb on - strand, within Pf1N1B4_4687 at 5179.135 kb on - strand, within Pf1N1B4_4687 at 5179.135 kb on - strand, within Pf1N1B4_4687 at 5179.135 kb on - strand, within Pf1N1B4_4687 at 5179.135 kb on - strand, within Pf1N1B4_4687 at 5179.167 kb on + strand, within Pf1N1B4_4687 at 5179.303 kb on + strand, within Pf1N1B4_4687 at 5179.303 kb on + strand, within Pf1N1B4_4687 at 5179.303 kb on + strand, within Pf1N1B4_4687 at 5179.303 kb on + strand, within Pf1N1B4_4687 at 5179.304 kb on - strand, within Pf1N1B4_4687 at 5179.339 kb on + strand, within Pf1N1B4_4687 at 5179.339 kb on + strand, within Pf1N1B4_4687 at 5179.341 kb on + strand, within Pf1N1B4_4687 at 5179.342 kb on - strand, within Pf1N1B4_4687 at 5179.342 kb on - strand, within Pf1N1B4_4687 at 5179.497 kb on + strand, within Pf1N1B4_4687 at 5179.542 kb on + strand at 5179.542 kb on + strand at 5179.543 kb on - strand, within Pf1N1B4_4687 at 5179.624 kb on + strand, within Pf1N1B4_4687 at 5179.625 kb on - strand, within Pf1N1B4_4687 at 5179.916 kb on + strand at 5179.916 kb on + strand at 5179.916 kb on + strand at 5179.917 kb on - strand at 5179.934 kb on - strand at 5179.934 kb on - strand at 5179.957 kb on + strand at 5179.957 kb on + strand at 5179.957 kb on + strand at 5179.963 kb on + strand at 5179.963 kb on + strand at 5179.963 kb on + strand at 5180.193 kb on + strand at 5180.205 kb on + strand at 5180.478 kb on + strand at 5180.536 kb on - strand at 5180.610 kb on + strand at 5180.611 kb on - strand at 5180.705 kb on + strand, within Pf1N1B4_4688 at 5180.706 kb on - strand, within Pf1N1B4_4688 at 5180.919 kb on + strand, within Pf1N1B4_4688 at 5180.919 kb on + strand, within Pf1N1B4_4688 at 5180.919 kb on + strand, within Pf1N1B4_4688 at 5180.919 kb on + strand, within Pf1N1B4_4688 at 5180.920 kb on - strand, within Pf1N1B4_4688 at 5180.920 kb on - strand, within Pf1N1B4_4688 at 5180.920 kb on - strand, within Pf1N1B4_4688
Per-strain Table
Position Strand Gene LocusTag Fraction D-Lactate (C) remove 5,177,351 + Pf1N1B4_4686 0.46 +2.4 5,177,351 + Pf1N1B4_4686 0.46 +1.0 5,177,739 - Pf1N1B4_4686 0.68 -1.2 5,177,862 + Pf1N1B4_4686 0.75 +0.7 5,177,984 + Pf1N1B4_4686 0.82 +0.6 5,177,984 + -1.7 5,178,115 - Pf1N1B4_4686 0.90 +0.4 5,178,115 - Pf1N1B4_4686 0.90 +0.3 5,178,197 - -2.0 5,178,348 + -1.2 5,178,348 + -2.0 5,178,349 - +0.4 5,178,393 + -1.0 5,178,631 - Pf1N1B4_4687 0.18 +1.8 5,178,837 + Pf1N1B4_4687 0.31 +0.5 5,178,837 + +0.8 5,178,837 + Pf1N1B4_4687 0.31 +2.1 5,178,838 - Pf1N1B4_4687 0.31 +0.4 5,179,134 + Pf1N1B4_4687 0.50 +1.2 5,179,134 + +0.8 5,179,135 - Pf1N1B4_4687 0.50 +0.3 5,179,135 - Pf1N1B4_4687 0.50 -0.4 5,179,135 - Pf1N1B4_4687 0.50 +1.0 5,179,135 - Pf1N1B4_4687 0.50 +0.6 5,179,135 - Pf1N1B4_4687 0.50 +0.8 5,179,167 + Pf1N1B4_4687 0.52 +1.8 5,179,303 + Pf1N1B4_4687 0.60 +1.5 5,179,303 + Pf1N1B4_4687 0.60 +1.6 5,179,303 + Pf1N1B4_4687 0.60 +0.8 5,179,303 + Pf1N1B4_4687 0.60 +0.0 5,179,304 - Pf1N1B4_4687 0.60 +0.4 5,179,339 + Pf1N1B4_4687 0.63 +0.8 5,179,339 + Pf1N1B4_4687 0.63 +0.1 5,179,341 + Pf1N1B4_4687 0.63 +1.4 5,179,342 - Pf1N1B4_4687 0.63 +0.3 5,179,342 - Pf1N1B4_4687 0.63 +0.3 5,179,497 + Pf1N1B4_4687 0.72 -0.8 5,179,542 + +1.8 5,179,542 + -0.2 5,179,543 - Pf1N1B4_4687 0.75 +1.2 5,179,624 + Pf1N1B4_4687 0.80 +1.8 5,179,625 - Pf1N1B4_4687 0.81 +1.2 5,179,916 + +0.5 5,179,916 + +0.2 5,179,916 + +1.2 5,179,917 - +0.4 5,179,934 - -0.4 5,179,934 - +0.4 5,179,957 + +1.0 5,179,957 + +0.7 5,179,957 + +2.1 5,179,963 + +0.1 5,179,963 + -0.0 5,179,963 + +1.8 5,180,193 + +1.1 5,180,205 + -0.2 5,180,478 + -1.0 5,180,536 - +1.4 5,180,610 + -2.6 5,180,611 - -1.0 5,180,705 + Pf1N1B4_4688 0.18 +0.6 5,180,706 - Pf1N1B4_4688 0.18 -0.0 5,180,919 + Pf1N1B4_4688 0.40 -0.8 5,180,919 + Pf1N1B4_4688 0.40 -0.2 5,180,919 + Pf1N1B4_4688 0.40 -1.5 5,180,919 + Pf1N1B4_4688 0.40 +0.3 5,180,920 - Pf1N1B4_4688 0.40 +0.8 5,180,920 - Pf1N1B4_4688 0.40 +0.5 5,180,920 - Pf1N1B4_4688 0.40 +0.2
Or see this region's nucleotide sequence