Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4687

Experiment: D-Lactate (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4686 and Pf1N1B4_4687 are separated by 55 nucleotidesPf1N1B4_4687 and Pf1N1B4_4688 are separated by 600 nucleotides Pf1N1B4_4686 - Malto-oligosyltrehalose trehalohydrolase (EC 3.2.1.141), at 5,176,537 to 5,178,291 _4686 Pf1N1B4_4687 - Glycogen synthase, ADP-glucose transglucosylase (EC 2.4.1.21), at 5,178,347 to 5,179,933 _4687 Pf1N1B4_4688 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 5,180,534 to 5,181,487 _4688 Position (kb) 5178 5179 5180Strain fitness (log2 ratio) -2 -1 0 1 2at 5177.351 kb on + strand, within Pf1N1B4_4686at 5177.351 kb on + strand, within Pf1N1B4_4686at 5177.739 kb on - strand, within Pf1N1B4_4686at 5177.862 kb on + strand, within Pf1N1B4_4686at 5177.984 kb on + strand, within Pf1N1B4_4686at 5177.984 kb on + strandat 5178.115 kb on - strand, within Pf1N1B4_4686at 5178.115 kb on - strand, within Pf1N1B4_4686at 5178.197 kb on - strandat 5178.348 kb on + strandat 5178.348 kb on + strandat 5178.349 kb on - strandat 5178.393 kb on + strandat 5178.631 kb on - strand, within Pf1N1B4_4687at 5178.837 kb on + strand, within Pf1N1B4_4687at 5178.837 kb on + strandat 5178.837 kb on + strand, within Pf1N1B4_4687at 5178.838 kb on - strand, within Pf1N1B4_4687at 5179.134 kb on + strand, within Pf1N1B4_4687at 5179.134 kb on + strandat 5179.135 kb on - strand, within Pf1N1B4_4687at 5179.135 kb on - strand, within Pf1N1B4_4687at 5179.135 kb on - strand, within Pf1N1B4_4687at 5179.135 kb on - strand, within Pf1N1B4_4687at 5179.135 kb on - strand, within Pf1N1B4_4687at 5179.167 kb on + strand, within Pf1N1B4_4687at 5179.303 kb on + strand, within Pf1N1B4_4687at 5179.303 kb on + strand, within Pf1N1B4_4687at 5179.303 kb on + strand, within Pf1N1B4_4687at 5179.303 kb on + strand, within Pf1N1B4_4687at 5179.304 kb on - strand, within Pf1N1B4_4687at 5179.339 kb on + strand, within Pf1N1B4_4687at 5179.339 kb on + strand, within Pf1N1B4_4687at 5179.341 kb on + strand, within Pf1N1B4_4687at 5179.342 kb on - strand, within Pf1N1B4_4687at 5179.342 kb on - strand, within Pf1N1B4_4687at 5179.497 kb on + strand, within Pf1N1B4_4687at 5179.542 kb on + strandat 5179.542 kb on + strandat 5179.543 kb on - strand, within Pf1N1B4_4687at 5179.624 kb on + strand, within Pf1N1B4_4687at 5179.625 kb on - strand, within Pf1N1B4_4687at 5179.916 kb on + strandat 5179.916 kb on + strandat 5179.916 kb on + strandat 5179.917 kb on - strandat 5179.934 kb on - strandat 5179.934 kb on - strandat 5179.957 kb on + strandat 5179.957 kb on + strandat 5179.957 kb on + strandat 5179.963 kb on + strandat 5179.963 kb on + strandat 5179.963 kb on + strandat 5180.193 kb on + strandat 5180.205 kb on + strandat 5180.478 kb on + strandat 5180.536 kb on - strandat 5180.610 kb on + strandat 5180.611 kb on - strandat 5180.705 kb on + strand, within Pf1N1B4_4688at 5180.706 kb on - strand, within Pf1N1B4_4688at 5180.919 kb on + strand, within Pf1N1B4_4688at 5180.919 kb on + strand, within Pf1N1B4_4688at 5180.919 kb on + strand, within Pf1N1B4_4688at 5180.919 kb on + strand, within Pf1N1B4_4688at 5180.920 kb on - strand, within Pf1N1B4_4688at 5180.920 kb on - strand, within Pf1N1B4_4688at 5180.920 kb on - strand, within Pf1N1B4_4688

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Lactate (C)
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5,177,351 + Pf1N1B4_4686 0.46 +2.4
5,177,351 + Pf1N1B4_4686 0.46 +1.0
5,177,739 - Pf1N1B4_4686 0.68 -1.2
5,177,862 + Pf1N1B4_4686 0.75 +0.7
5,177,984 + Pf1N1B4_4686 0.82 +0.6
5,177,984 + -1.7
5,178,115 - Pf1N1B4_4686 0.90 +0.4
5,178,115 - Pf1N1B4_4686 0.90 +0.3
5,178,197 - -2.0
5,178,348 + -1.2
5,178,348 + -2.0
5,178,349 - +0.4
5,178,393 + -1.0
5,178,631 - Pf1N1B4_4687 0.18 +1.8
5,178,837 + Pf1N1B4_4687 0.31 +0.5
5,178,837 + +0.8
5,178,837 + Pf1N1B4_4687 0.31 +2.1
5,178,838 - Pf1N1B4_4687 0.31 +0.4
5,179,134 + Pf1N1B4_4687 0.50 +1.2
5,179,134 + +0.8
5,179,135 - Pf1N1B4_4687 0.50 +0.3
5,179,135 - Pf1N1B4_4687 0.50 -0.4
5,179,135 - Pf1N1B4_4687 0.50 +1.0
5,179,135 - Pf1N1B4_4687 0.50 +0.6
5,179,135 - Pf1N1B4_4687 0.50 +0.8
5,179,167 + Pf1N1B4_4687 0.52 +1.8
5,179,303 + Pf1N1B4_4687 0.60 +1.5
5,179,303 + Pf1N1B4_4687 0.60 +1.6
5,179,303 + Pf1N1B4_4687 0.60 +0.8
5,179,303 + Pf1N1B4_4687 0.60 +0.0
5,179,304 - Pf1N1B4_4687 0.60 +0.4
5,179,339 + Pf1N1B4_4687 0.63 +0.8
5,179,339 + Pf1N1B4_4687 0.63 +0.1
5,179,341 + Pf1N1B4_4687 0.63 +1.4
5,179,342 - Pf1N1B4_4687 0.63 +0.3
5,179,342 - Pf1N1B4_4687 0.63 +0.3
5,179,497 + Pf1N1B4_4687 0.72 -0.8
5,179,542 + +1.8
5,179,542 + -0.2
5,179,543 - Pf1N1B4_4687 0.75 +1.2
5,179,624 + Pf1N1B4_4687 0.80 +1.8
5,179,625 - Pf1N1B4_4687 0.81 +1.2
5,179,916 + +0.5
5,179,916 + +0.2
5,179,916 + +1.2
5,179,917 - +0.4
5,179,934 - -0.4
5,179,934 - +0.4
5,179,957 + +1.0
5,179,957 + +0.7
5,179,957 + +2.1
5,179,963 + +0.1
5,179,963 + -0.0
5,179,963 + +1.8
5,180,193 + +1.1
5,180,205 + -0.2
5,180,478 + -1.0
5,180,536 - +1.4
5,180,610 + -2.6
5,180,611 - -1.0
5,180,705 + Pf1N1B4_4688 0.18 +0.6
5,180,706 - Pf1N1B4_4688 0.18 -0.0
5,180,919 + Pf1N1B4_4688 0.40 -0.8
5,180,919 + Pf1N1B4_4688 0.40 -0.2
5,180,919 + Pf1N1B4_4688 0.40 -1.5
5,180,919 + Pf1N1B4_4688 0.40 +0.3
5,180,920 - Pf1N1B4_4688 0.40 +0.8
5,180,920 - Pf1N1B4_4688 0.40 +0.5
5,180,920 - Pf1N1B4_4688 0.40 +0.2

Or see this region's nucleotide sequence