Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4624

Experiment: D-Lactate (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4623 and Pf1N1B4_4624 are separated by 10 nucleotidesPf1N1B4_4624 and Pf1N1B4_4625 are separated by 199 nucleotidesPf1N1B4_4625 and Pf1N1B4_4626 overlap by 14 nucleotides Pf1N1B4_4623 - 2-dehydro-3-deoxy-L-arabinonate dehydratase (EC 4.2.1.43) (from data), at 5,099,613 to 5,100,605 _4623 Pf1N1B4_4624 - Ketoglutarate semialdehyde dehydrogenase (EC 1.2.1.26), at 5,100,616 to 5,102,196 _4624 Pf1N1B4_4625 - FIG00955242: hypothetical protein, at 5,102,396 to 5,102,692 _4625 Pf1N1B4_4626 - 3-carboxymuconate cyclase, at 5,102,679 to 5,103,896 _4626 Position (kb) 5100 5101 5102 5103Strain fitness (log2 ratio) -1 0 1 2at 5099.793 kb on + strandat 5100.030 kb on + strand, within Pf1N1B4_4623at 5100.031 kb on - strand, within Pf1N1B4_4623at 5100.031 kb on - strand, within Pf1N1B4_4623at 5100.130 kb on - strand, within Pf1N1B4_4623at 5100.217 kb on - strand, within Pf1N1B4_4623at 5100.217 kb on - strandat 5100.223 kb on - strand, within Pf1N1B4_4623at 5100.255 kb on + strand, within Pf1N1B4_4623at 5100.411 kb on + strand, within Pf1N1B4_4623at 5100.411 kb on + strand, within Pf1N1B4_4623at 5100.640 kb on + strandat 5100.641 kb on - strandat 5100.641 kb on - strandat 5100.641 kb on - strandat 5100.912 kb on + strand, within Pf1N1B4_4624at 5100.913 kb on - strand, within Pf1N1B4_4624at 5101.020 kb on + strand, within Pf1N1B4_4624at 5101.051 kb on + strand, within Pf1N1B4_4624at 5101.064 kb on + strand, within Pf1N1B4_4624at 5101.642 kb on + strand, within Pf1N1B4_4624at 5101.642 kb on + strand, within Pf1N1B4_4624at 5101.776 kb on - strand, within Pf1N1B4_4624at 5102.140 kb on - strandat 5102.361 kb on - strandat 5102.361 kb on - strandat 5102.361 kb on - strandat 5102.782 kb on - strandat 5103.094 kb on - strand, within Pf1N1B4_4626at 5103.094 kb on - strandat 5103.094 kb on - strandat 5103.157 kb on + strandat 5103.158 kb on - strand, within Pf1N1B4_4626

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Lactate (C)
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5,099,793 + -1.2
5,100,030 + Pf1N1B4_4623 0.42 -1.0
5,100,031 - Pf1N1B4_4623 0.42 -1.2
5,100,031 - Pf1N1B4_4623 0.42 -0.7
5,100,130 - Pf1N1B4_4623 0.52 -1.0
5,100,217 - Pf1N1B4_4623 0.61 +0.1
5,100,217 - +0.1
5,100,223 - Pf1N1B4_4623 0.61 +0.5
5,100,255 + Pf1N1B4_4623 0.65 +1.3
5,100,411 + Pf1N1B4_4623 0.80 +0.2
5,100,411 + Pf1N1B4_4623 0.80 +0.7
5,100,640 + -0.7
5,100,641 - -1.3
5,100,641 - -1.6
5,100,641 - -1.3
5,100,912 + Pf1N1B4_4624 0.19 +0.2
5,100,913 - Pf1N1B4_4624 0.19 +0.8
5,101,020 + Pf1N1B4_4624 0.26 +0.3
5,101,051 + Pf1N1B4_4624 0.28 +1.8
5,101,064 + Pf1N1B4_4624 0.28 +0.3
5,101,642 + Pf1N1B4_4624 0.65 -0.4
5,101,642 + Pf1N1B4_4624 0.65 +1.4
5,101,776 - Pf1N1B4_4624 0.73 +0.3
5,102,140 - -0.2
5,102,361 - -1.0
5,102,361 - +0.3
5,102,361 - +0.0
5,102,782 - -1.7
5,103,094 - Pf1N1B4_4626 0.34 -1.3
5,103,094 - -0.2
5,103,094 - +0.8
5,103,157 + +0.4
5,103,158 - Pf1N1B4_4626 0.39 +0.8

Or see this region's nucleotide sequence