Experiment: D-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4271 and Pf1N1B4_4272 are separated by 173 nucleotides Pf1N1B4_4272 and Pf1N1B4_4273 are separated by 74 nucleotides Pf1N1B4_4273 and Pf1N1B4_4274 are separated by 311 nucleotides
Pf1N1B4_4271 - Hydrogen cyanide synthase HcnC / Opine oxidase subunit B, at 4,735,047 to 4,736,306
_4271
Pf1N1B4_4272 - Glutathione S-transferase, unnamed subgroup 2 (EC 2.5.1.18), at 4,736,480 to 4,737,091
_4272
Pf1N1B4_4273 - Predicted transcriptional regulator of the myo-inositol catabolic operon, at 4,737,166 to 4,738,086
_4273
Pf1N1B4_4274 - 5-keto-2-deoxygluconokinase (EC 2.7.1.92) / uncharacterized domain, at 4,738,398 to 4,740,335
_4274
Position (kb)
4737
4738
4739 Strain fitness (log2 ratio)
-2
-1
0
1 at 4736.256 kb on - strand at 4736.335 kb on + strand at 4736.353 kb on + strand at 4736.524 kb on - strand at 4736.524 kb on - strand at 4736.615 kb on - strand at 4737.060 kb on + strand at 4737.131 kb on + strand at 4737.132 kb on - strand at 4737.220 kb on - strand at 4737.220 kb on - strand at 4737.221 kb on + strand at 4737.222 kb on - strand at 4737.539 kb on + strand, within Pf1N1B4_4273 at 4737.539 kb on + strand, within Pf1N1B4_4273 at 4737.587 kb on + strand, within Pf1N1B4_4273 at 4737.587 kb on + strand at 4737.587 kb on + strand, within Pf1N1B4_4273 at 4737.587 kb on + strand at 4737.588 kb on - strand, within Pf1N1B4_4273 at 4737.761 kb on + strand, within Pf1N1B4_4273 at 4737.875 kb on - strand, within Pf1N1B4_4273 at 4737.875 kb on - strand, within Pf1N1B4_4273 at 4737.941 kb on + strand, within Pf1N1B4_4273 at 4737.941 kb on + strand at 4737.942 kb on - strand, within Pf1N1B4_4273 at 4737.942 kb on - strand, within Pf1N1B4_4273 at 4737.942 kb on - strand, within Pf1N1B4_4273 at 4737.980 kb on + strand, within Pf1N1B4_4273 at 4737.981 kb on - strand, within Pf1N1B4_4273 at 4738.087 kb on - strand at 4738.144 kb on + strand at 4738.144 kb on + strand at 4738.145 kb on - strand at 4738.145 kb on - strand at 4738.456 kb on + strand at 4738.470 kb on + strand at 4738.521 kb on + strand at 4738.884 kb on + strand, within Pf1N1B4_4274 at 4738.884 kb on + strand, within Pf1N1B4_4274 at 4738.884 kb on + strand, within Pf1N1B4_4274
Per-strain Table
Position Strand Gene LocusTag Fraction D-Lactate (C) remove 4,736,256 - -2.0 4,736,335 + -1.0 4,736,353 + +0.0 4,736,524 - +0.1 4,736,524 - +0.8 4,736,615 - +0.8 4,737,060 + -1.2 4,737,131 + -1.6 4,737,132 - -0.6 4,737,220 - +0.2 4,737,220 - -0.5 4,737,221 + +0.0 4,737,222 - +0.5 4,737,539 + Pf1N1B4_4273 0.40 -1.5 4,737,539 + Pf1N1B4_4273 0.40 -0.2 4,737,587 + Pf1N1B4_4273 0.46 -0.6 4,737,587 + +0.8 4,737,587 + Pf1N1B4_4273 0.46 -1.2 4,737,587 + -0.8 4,737,588 - Pf1N1B4_4273 0.46 -1.2 4,737,761 + Pf1N1B4_4273 0.65 -0.8 4,737,875 - Pf1N1B4_4273 0.77 +0.2 4,737,875 - Pf1N1B4_4273 0.77 -0.8 4,737,941 + Pf1N1B4_4273 0.84 -1.4 4,737,941 + -0.8 4,737,942 - Pf1N1B4_4273 0.84 +0.8 4,737,942 - Pf1N1B4_4273 0.84 -0.3 4,737,942 - Pf1N1B4_4273 0.84 -1.6 4,737,980 + Pf1N1B4_4273 0.88 +0.0 4,737,981 - Pf1N1B4_4273 0.88 -1.2 4,738,087 - -2.0 4,738,144 + -0.5 4,738,144 + +0.0 4,738,145 - -0.3 4,738,145 - -1.2 4,738,456 + +0.8 4,738,470 + +0.8 4,738,521 + -2.0 4,738,884 + Pf1N1B4_4274 0.25 +1.1 4,738,884 + Pf1N1B4_4274 0.25 +0.4 4,738,884 + Pf1N1B4_4274 0.25 +0.2
Or see this region's nucleotide sequence