Experiment: D-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4266 and Pf1N1B4_4267 overlap by 23 nucleotides Pf1N1B4_4267 and Pf1N1B4_4268 are separated by 269 nucleotides Pf1N1B4_4268 and Pf1N1B4_4269 are separated by 341 nucleotides Pf1N1B4_4269 and Pf1N1B4_4270 overlap by 4 nucleotides
Pf1N1B4_4266 - sensor histidine kinase, at 4,729,669 to 4,731,063
_4266
Pf1N1B4_4267 - DNA-binding response regulator, at 4,731,041 to 4,731,724
_4267
Pf1N1B4_4268 - Mll5186 protein, at 4,731,994 to 4,732,944
_4268
Pf1N1B4_4269 - Hydrogen cyanide synthase HcnA / Opine oxidase subunit C, at 4,733,286 to 4,733,648
_4269
Pf1N1B4_4270 - Hydrogen cyanide synthase HcnB / Opine oxidase subunit A, at 4,733,645 to 4,735,054
_4270
Position (kb)
4731
4732
4733 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4731.003 kb on - strand at 4731.064 kb on - strand at 4731.252 kb on + strand, within Pf1N1B4_4267 at 4731.739 kb on + strand at 4731.830 kb on + strand at 4731.919 kb on + strand at 4731.968 kb on + strand at 4731.969 kb on - strand at 4731.971 kb on + strand at 4731.971 kb on + strand at 4731.971 kb on + strand at 4731.971 kb on + strand at 4732.068 kb on + strand at 4732.115 kb on + strand, within Pf1N1B4_4268 at 4732.191 kb on + strand, within Pf1N1B4_4268 at 4732.249 kb on + strand, within Pf1N1B4_4268 at 4732.249 kb on + strand, within Pf1N1B4_4268 at 4732.249 kb on + strand, within Pf1N1B4_4268 at 4732.282 kb on + strand, within Pf1N1B4_4268 at 4732.283 kb on - strand, within Pf1N1B4_4268 at 4732.530 kb on + strand, within Pf1N1B4_4268 at 4732.530 kb on + strand, within Pf1N1B4_4268 at 4732.633 kb on + strand, within Pf1N1B4_4268 at 4732.633 kb on + strand, within Pf1N1B4_4268 at 4732.706 kb on - strand, within Pf1N1B4_4268 at 4732.882 kb on + strand at 4732.900 kb on + strand at 4732.901 kb on - strand at 4732.901 kb on - strand at 4732.901 kb on - strand at 4732.968 kb on + strand at 4732.968 kb on + strand at 4732.968 kb on + strand at 4732.968 kb on + strand at 4732.968 kb on + strand at 4732.968 kb on + strand at 4732.969 kb on - strand at 4732.969 kb on - strand at 4732.969 kb on - strand at 4732.970 kb on + strand at 4732.971 kb on - strand at 4732.971 kb on - strand at 4733.226 kb on - strand at 4733.230 kb on + strand at 4733.231 kb on - strand at 4733.276 kb on + strand at 4733.276 kb on + strand at 4733.276 kb on + strand at 4733.277 kb on - strand at 4733.277 kb on - strand at 4733.304 kb on + strand at 4733.508 kb on + strand, within Pf1N1B4_4269 at 4733.644 kb on + strand at 4733.728 kb on + strand at 4733.728 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Lactate (C) remove 4,731,003 - -0.8 4,731,064 - +0.5 4,731,252 + Pf1N1B4_4267 0.31 -2.0 4,731,739 + -0.8 4,731,830 + +1.8 4,731,919 + -0.8 4,731,968 + -2.0 4,731,969 - -0.2 4,731,971 + +0.2 4,731,971 + -0.2 4,731,971 + +0.4 4,731,971 + +0.4 4,732,068 + +0.0 4,732,115 + Pf1N1B4_4268 0.13 +0.2 4,732,191 + Pf1N1B4_4268 0.21 +0.5 4,732,249 + Pf1N1B4_4268 0.27 +0.5 4,732,249 + Pf1N1B4_4268 0.27 +1.2 4,732,249 + Pf1N1B4_4268 0.27 +0.1 4,732,282 + Pf1N1B4_4268 0.30 +0.6 4,732,283 - Pf1N1B4_4268 0.30 -0.5 4,732,530 + Pf1N1B4_4268 0.56 -2.1 4,732,530 + Pf1N1B4_4268 0.56 -0.5 4,732,633 + Pf1N1B4_4268 0.67 +1.2 4,732,633 + Pf1N1B4_4268 0.67 -0.6 4,732,706 - Pf1N1B4_4268 0.75 +1.5 4,732,882 + -0.8 4,732,900 + -0.9 4,732,901 - -1.2 4,732,901 - -1.3 4,732,901 - +0.8 4,732,968 + -0.8 4,732,968 + +0.1 4,732,968 + -0.4 4,732,968 + +0.2 4,732,968 + +1.4 4,732,968 + -1.5 4,732,969 - -1.8 4,732,969 - +1.4 4,732,969 - +0.8 4,732,970 + -0.0 4,732,971 - +0.2 4,732,971 - -0.9 4,733,226 - -0.2 4,733,230 + -0.8 4,733,231 - -1.0 4,733,276 + -0.3 4,733,276 + +1.1 4,733,276 + -1.8 4,733,277 - +0.8 4,733,277 - +1.1 4,733,304 + -0.8 4,733,508 + Pf1N1B4_4269 0.61 -1.6 4,733,644 + -0.2 4,733,728 + -0.2 4,733,728 + -1.0
Or see this region's nucleotide sequence