Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4268

Experiment: D-Lactate (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4266 and Pf1N1B4_4267 overlap by 23 nucleotidesPf1N1B4_4267 and Pf1N1B4_4268 are separated by 269 nucleotidesPf1N1B4_4268 and Pf1N1B4_4269 are separated by 341 nucleotidesPf1N1B4_4269 and Pf1N1B4_4270 overlap by 4 nucleotides Pf1N1B4_4266 - sensor histidine kinase, at 4,729,669 to 4,731,063 _4266 Pf1N1B4_4267 - DNA-binding response regulator, at 4,731,041 to 4,731,724 _4267 Pf1N1B4_4268 - Mll5186 protein, at 4,731,994 to 4,732,944 _4268 Pf1N1B4_4269 - Hydrogen cyanide synthase HcnA / Opine oxidase subunit C, at 4,733,286 to 4,733,648 _4269 Pf1N1B4_4270 - Hydrogen cyanide synthase HcnB / Opine oxidase subunit A, at 4,733,645 to 4,735,054 _4270 Position (kb) 4731 4732 4733Strain fitness (log2 ratio) -2 -1 0 1 2at 4731.003 kb on - strandat 4731.064 kb on - strandat 4731.252 kb on + strand, within Pf1N1B4_4267at 4731.739 kb on + strandat 4731.830 kb on + strandat 4731.919 kb on + strandat 4731.968 kb on + strandat 4731.969 kb on - strandat 4731.971 kb on + strandat 4731.971 kb on + strandat 4731.971 kb on + strandat 4731.971 kb on + strandat 4732.068 kb on + strandat 4732.115 kb on + strand, within Pf1N1B4_4268at 4732.191 kb on + strand, within Pf1N1B4_4268at 4732.249 kb on + strand, within Pf1N1B4_4268at 4732.249 kb on + strand, within Pf1N1B4_4268at 4732.249 kb on + strand, within Pf1N1B4_4268at 4732.282 kb on + strand, within Pf1N1B4_4268at 4732.283 kb on - strand, within Pf1N1B4_4268at 4732.530 kb on + strand, within Pf1N1B4_4268at 4732.530 kb on + strand, within Pf1N1B4_4268at 4732.633 kb on + strand, within Pf1N1B4_4268at 4732.633 kb on + strand, within Pf1N1B4_4268at 4732.706 kb on - strand, within Pf1N1B4_4268at 4732.882 kb on + strandat 4732.900 kb on + strandat 4732.901 kb on - strandat 4732.901 kb on - strandat 4732.901 kb on - strandat 4732.968 kb on + strandat 4732.968 kb on + strandat 4732.968 kb on + strandat 4732.968 kb on + strandat 4732.968 kb on + strandat 4732.968 kb on + strandat 4732.969 kb on - strandat 4732.969 kb on - strandat 4732.969 kb on - strandat 4732.970 kb on + strandat 4732.971 kb on - strandat 4732.971 kb on - strandat 4733.226 kb on - strandat 4733.230 kb on + strandat 4733.231 kb on - strandat 4733.276 kb on + strandat 4733.276 kb on + strandat 4733.276 kb on + strandat 4733.277 kb on - strandat 4733.277 kb on - strandat 4733.304 kb on + strandat 4733.508 kb on + strand, within Pf1N1B4_4269at 4733.644 kb on + strandat 4733.728 kb on + strandat 4733.728 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Lactate (C)
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4,731,003 - -0.8
4,731,064 - +0.5
4,731,252 + Pf1N1B4_4267 0.31 -2.0
4,731,739 + -0.8
4,731,830 + +1.8
4,731,919 + -0.8
4,731,968 + -2.0
4,731,969 - -0.2
4,731,971 + +0.2
4,731,971 + -0.2
4,731,971 + +0.4
4,731,971 + +0.4
4,732,068 + +0.0
4,732,115 + Pf1N1B4_4268 0.13 +0.2
4,732,191 + Pf1N1B4_4268 0.21 +0.5
4,732,249 + Pf1N1B4_4268 0.27 +0.5
4,732,249 + Pf1N1B4_4268 0.27 +1.2
4,732,249 + Pf1N1B4_4268 0.27 +0.1
4,732,282 + Pf1N1B4_4268 0.30 +0.6
4,732,283 - Pf1N1B4_4268 0.30 -0.5
4,732,530 + Pf1N1B4_4268 0.56 -2.1
4,732,530 + Pf1N1B4_4268 0.56 -0.5
4,732,633 + Pf1N1B4_4268 0.67 +1.2
4,732,633 + Pf1N1B4_4268 0.67 -0.6
4,732,706 - Pf1N1B4_4268 0.75 +1.5
4,732,882 + -0.8
4,732,900 + -0.9
4,732,901 - -1.2
4,732,901 - -1.3
4,732,901 - +0.8
4,732,968 + -0.8
4,732,968 + +0.1
4,732,968 + -0.4
4,732,968 + +0.2
4,732,968 + +1.4
4,732,968 + -1.5
4,732,969 - -1.8
4,732,969 - +1.4
4,732,969 - +0.8
4,732,970 + -0.0
4,732,971 - +0.2
4,732,971 - -0.9
4,733,226 - -0.2
4,733,230 + -0.8
4,733,231 - -1.0
4,733,276 + -0.3
4,733,276 + +1.1
4,733,276 + -1.8
4,733,277 - +0.8
4,733,277 - +1.1
4,733,304 + -0.8
4,733,508 + Pf1N1B4_4269 0.61 -1.6
4,733,644 + -0.2
4,733,728 + -0.2
4,733,728 + -1.0

Or see this region's nucleotide sequence