Experiment: D-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_394 and Pf1N1B4_395 are separated by 227 nucleotides Pf1N1B4_395 and Pf1N1B4_396 overlap by 4 nucleotides Pf1N1B4_396 and Pf1N1B4_397 are separated by 60 nucleotides
Pf1N1B4_394 - Transcriptional regulator, AraC family, at 463,011 to 463,901
_394
Pf1N1B4_395 - Alcohol dehydrogenase (EC 1.1.1.1), at 464,129 to 465,181
_395
Pf1N1B4_396 - D-galactonate regulator, IclR family, at 465,178 to 465,981
_396
Pf1N1B4_397 - Hexuronate transporter, at 466,042 to 467,352
_397
Position (kb) 464
465
466 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 463.174 kb on + strand, within Pf1N1B4_394 at 463.174 kb on + strand, within Pf1N1B4_394 at 463.175 kb on - strand, within Pf1N1B4_394 at 463.175 kb on - strand, within Pf1N1B4_394 at 463.279 kb on + strand, within Pf1N1B4_394 at 463.279 kb on + strand, within Pf1N1B4_394 at 463.280 kb on - strand, within Pf1N1B4_394 at 463.280 kb on - strand, within Pf1N1B4_394 at 463.353 kb on + strand, within Pf1N1B4_394 at 463.353 kb on + strand, within Pf1N1B4_394 at 463.354 kb on - strand, within Pf1N1B4_394 at 463.354 kb on - strand, within Pf1N1B4_394 at 463.354 kb on - strand, within Pf1N1B4_394 at 463.354 kb on - strand, within Pf1N1B4_394 at 463.354 kb on - strand, within Pf1N1B4_394 at 463.354 kb on - strand, within Pf1N1B4_394 at 463.670 kb on - strand, within Pf1N1B4_394 at 463.684 kb on + strand, within Pf1N1B4_394 at 463.684 kb on + strand, within Pf1N1B4_394 at 463.708 kb on + strand, within Pf1N1B4_394 at 463.709 kb on - strand, within Pf1N1B4_394 at 463.709 kb on - strand, within Pf1N1B4_394 at 463.709 kb on - strand, within Pf1N1B4_394 at 463.754 kb on - strand, within Pf1N1B4_394 at 464.035 kb on + strand at 464.035 kb on + strand at 464.035 kb on + strand at 464.035 kb on + strand at 464.132 kb on + strand at 464.132 kb on + strand at 464.132 kb on + strand at 464.133 kb on - strand at 464.133 kb on - strand at 464.133 kb on - strand at 464.133 kb on - strand at 464.133 kb on - strand at 464.133 kb on - strand at 464.133 kb on - strand at 464.133 kb on - strand at 464.133 kb on - strand at 464.297 kb on + strand, within Pf1N1B4_395 at 464.298 kb on - strand, within Pf1N1B4_395 at 464.356 kb on + strand, within Pf1N1B4_395 at 464.359 kb on + strand at 464.359 kb on + strand, within Pf1N1B4_395 at 464.359 kb on + strand, within Pf1N1B4_395 at 464.359 kb on + strand, within Pf1N1B4_395 at 464.360 kb on - strand, within Pf1N1B4_395 at 464.360 kb on - strand, within Pf1N1B4_395 at 464.360 kb on - strand, within Pf1N1B4_395 at 464.448 kb on - strand, within Pf1N1B4_395 at 464.474 kb on + strand, within Pf1N1B4_395 at 464.474 kb on + strand, within Pf1N1B4_395 at 464.475 kb on - strand, within Pf1N1B4_395 at 464.475 kb on - strand, within Pf1N1B4_395 at 464.621 kb on + strand, within Pf1N1B4_395 at 464.622 kb on - strand, within Pf1N1B4_395 at 464.639 kb on + strand, within Pf1N1B4_395 at 464.640 kb on - strand, within Pf1N1B4_395 at 464.919 kb on + strand at 464.919 kb on + strand, within Pf1N1B4_395 at 464.919 kb on + strand, within Pf1N1B4_395 at 464.920 kb on - strand, within Pf1N1B4_395 at 464.920 kb on - strand, within Pf1N1B4_395 at 465.113 kb on + strand at 465.113 kb on + strand at 465.113 kb on + strand at 465.113 kb on + strand at 465.113 kb on + strand at 465.113 kb on + strand at 465.114 kb on - strand at 465.114 kb on - strand at 465.114 kb on - strand at 465.114 kb on - strand at 465.114 kb on - strand at 465.114 kb on - strand at 465.114 kb on - strand at 465.143 kb on + strand at 465.144 kb on - strand at 465.144 kb on - strand at 465.144 kb on - strand at 465.180 kb on - strand at 465.266 kb on + strand, within Pf1N1B4_396 at 465.266 kb on + strand, within Pf1N1B4_396 at 465.266 kb on + strand, within Pf1N1B4_396 at 465.267 kb on - strand, within Pf1N1B4_396 at 465.267 kb on - strand, within Pf1N1B4_396 at 465.393 kb on - strand, within Pf1N1B4_396 at 465.393 kb on - strand, within Pf1N1B4_396 at 465.486 kb on - strand, within Pf1N1B4_396 at 465.617 kb on + strand, within Pf1N1B4_396 at 465.618 kb on - strand, within Pf1N1B4_396 at 465.767 kb on + strand, within Pf1N1B4_396 at 465.794 kb on + strand, within Pf1N1B4_396 at 465.794 kb on + strand, within Pf1N1B4_396 at 465.795 kb on - strand, within Pf1N1B4_396 at 465.835 kb on - strand, within Pf1N1B4_396 at 466.021 kb on - strand at 466.021 kb on - strand at 466.044 kb on - strand at 466.054 kb on - strand at 466.082 kb on + strand at 466.083 kb on - strand at 466.116 kb on + strand at 466.116 kb on + strand at 466.117 kb on - strand at 466.117 kb on - strand at 466.117 kb on - strand at 466.117 kb on - strand at 466.117 kb on - strand at 466.117 kb on - strand at 466.118 kb on + strand at 466.118 kb on + strand at 466.118 kb on + strand at 466.118 kb on + strand at 466.118 kb on + strand at 466.119 kb on - strand at 466.119 kb on - strand at 466.119 kb on - strand at 466.119 kb on - strand at 466.119 kb on - strand at 466.119 kb on - strand at 466.119 kb on - strand at 466.119 kb on - strand at 466.119 kb on - strand at 466.119 kb on - strand at 466.119 kb on - strand at 466.119 kb on - strand at 466.119 kb on - strand at 466.119 kb on - strand at 466.119 kb on - strand at 466.119 kb on - strand at 466.119 kb on - strand at 466.119 kb on - strand at 466.119 kb on - strand at 466.119 kb on - strand at 466.119 kb on - strand at 466.119 kb on - strand at 466.123 kb on - strand at 466.179 kb on + strand, within Pf1N1B4_397
Per-strain Table
Position Strand Gene LocusTag Fraction D-Lactate (C) remove 463,174 + Pf1N1B4_394 0.18 -1.0 463,174 + Pf1N1B4_394 0.18 -2.3 463,175 - Pf1N1B4_394 0.18 +0.5 463,175 - Pf1N1B4_394 0.18 +0.8 463,279 + Pf1N1B4_394 0.30 -0.1 463,279 + Pf1N1B4_394 0.30 +0.1 463,280 - Pf1N1B4_394 0.30 +0.1 463,280 - Pf1N1B4_394 0.30 +1.1 463,353 + Pf1N1B4_394 0.38 +0.7 463,353 + Pf1N1B4_394 0.38 -0.6 463,354 - Pf1N1B4_394 0.38 -0.5 463,354 - Pf1N1B4_394 0.38 +0.1 463,354 - Pf1N1B4_394 0.38 -0.4 463,354 - Pf1N1B4_394 0.38 -1.2 463,354 - Pf1N1B4_394 0.38 -0.5 463,354 - Pf1N1B4_394 0.38 -0.7 463,670 - Pf1N1B4_394 0.74 +1.1 463,684 + Pf1N1B4_394 0.76 -1.2 463,684 + Pf1N1B4_394 0.76 +3.7 463,708 + Pf1N1B4_394 0.78 -0.7 463,709 - Pf1N1B4_394 0.78 +0.3 463,709 - Pf1N1B4_394 0.78 -0.3 463,709 - Pf1N1B4_394 0.78 +0.4 463,754 - Pf1N1B4_394 0.83 -0.2 464,035 + -0.3 464,035 + +0.2 464,035 + -0.2 464,035 + -0.1 464,132 + +0.5 464,132 + -0.9 464,132 + +0.1 464,133 - -0.2 464,133 - +0.5 464,133 - -0.7 464,133 - -1.1 464,133 - -0.5 464,133 - +0.4 464,133 - -0.1 464,133 - +0.8 464,133 - -1.8 464,297 + Pf1N1B4_395 0.16 -0.6 464,298 - Pf1N1B4_395 0.16 +0.2 464,356 + Pf1N1B4_395 0.22 -0.5 464,359 + -1.2 464,359 + Pf1N1B4_395 0.22 +0.4 464,359 + Pf1N1B4_395 0.22 -1.6 464,359 + Pf1N1B4_395 0.22 +0.5 464,360 - Pf1N1B4_395 0.22 -0.7 464,360 - Pf1N1B4_395 0.22 -0.1 464,360 - Pf1N1B4_395 0.22 +0.5 464,448 - Pf1N1B4_395 0.30 +1.1 464,474 + Pf1N1B4_395 0.33 -0.1 464,474 + Pf1N1B4_395 0.33 +0.3 464,475 - Pf1N1B4_395 0.33 -0.1 464,475 - Pf1N1B4_395 0.33 -1.0 464,621 + Pf1N1B4_395 0.47 -0.5 464,622 - Pf1N1B4_395 0.47 +0.1 464,639 + Pf1N1B4_395 0.48 -0.9 464,640 - Pf1N1B4_395 0.49 -0.2 464,919 + +3.4 464,919 + Pf1N1B4_395 0.75 +1.1 464,919 + Pf1N1B4_395 0.75 +0.7 464,920 - Pf1N1B4_395 0.75 -0.8 464,920 - Pf1N1B4_395 0.75 -0.8 465,113 + +2.1 465,113 + -0.3 465,113 + +1.7 465,113 + +0.1 465,113 + +0.4 465,113 + +1.1 465,114 - -1.7 465,114 - +0.3 465,114 - +1.5 465,114 - +0.1 465,114 - +0.4 465,114 - -0.2 465,114 - +1.7 465,143 + -1.5 465,144 - +1.1 465,144 - -0.3 465,144 - +1.1 465,180 - -0.7 465,266 + Pf1N1B4_396 0.11 +0.2 465,266 + Pf1N1B4_396 0.11 -0.7 465,266 + Pf1N1B4_396 0.11 -2.1 465,267 - Pf1N1B4_396 0.11 -1.0 465,267 - Pf1N1B4_396 0.11 -1.9 465,393 - Pf1N1B4_396 0.27 -0.2 465,393 - Pf1N1B4_396 0.27 -0.4 465,486 - Pf1N1B4_396 0.38 +0.9 465,617 + Pf1N1B4_396 0.55 -1.7 465,618 - Pf1N1B4_396 0.55 -1.7 465,767 + Pf1N1B4_396 0.73 -2.0 465,794 + Pf1N1B4_396 0.77 +0.8 465,794 + Pf1N1B4_396 0.77 +0.1 465,795 - Pf1N1B4_396 0.77 -2.1 465,835 - Pf1N1B4_396 0.82 -0.6 466,021 - +0.3 466,021 - -0.1 466,044 - +0.5 466,054 - -0.3 466,082 + -0.5 466,083 - -0.2 466,116 + -0.5 466,116 + -1.0 466,117 - -1.2 466,117 - +0.3 466,117 - -0.8 466,117 - -0.3 466,117 - -0.5 466,117 - +0.5 466,118 + +1.1 466,118 + -0.3 466,118 + -0.5 466,118 + -0.5 466,118 + -0.7 466,119 - +0.1 466,119 - -0.5 466,119 - -2.7 466,119 - +1.9 466,119 - +0.4 466,119 - -0.9 466,119 - +0.4 466,119 - +0.0 466,119 - -0.5 466,119 - -0.2 466,119 - -0.9 466,119 - -0.3 466,119 - -1.2 466,119 - -1.3 466,119 - -1.6 466,119 - +0.3 466,119 - +0.7 466,119 - +0.8 466,119 - -0.2 466,119 - -0.8 466,119 - +1.1 466,119 - -2.1 466,123 - -1.5 466,179 + Pf1N1B4_397 0.10 +0.2
Or see this region's nucleotide sequence