Experiment: D-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3365 and Pf1N1B4_3366 are separated by 78 nucleotides Pf1N1B4_3366 and Pf1N1B4_3367 overlap by 4 nucleotides Pf1N1B4_3367 and Pf1N1B4_3368 overlap by 4 nucleotides
Pf1N1B4_3365 - Sialic acid transporter (permease) NanT, at 3,753,013 to 3,754,296
_3365
Pf1N1B4_3366 - Lactam utilization protein LamB, at 3,754,375 to 3,755,127
_3366
Pf1N1B4_3367 - Allophanate hydrolase 2 subunit 1 (EC 3.5.1.54), at 3,755,124 to 3,755,828
_3367
Pf1N1B4_3368 - Allophanate hydrolase 2 subunit 2 (EC 3.5.1.54), at 3,755,825 to 3,756,751
_3368
Position (kb)
3755
3756 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3754.316 kb on + strand at 3754.330 kb on + strand at 3754.330 kb on + strand at 3754.330 kb on + strand at 3754.330 kb on + strand at 3754.330 kb on + strand at 3754.331 kb on - strand at 3754.331 kb on - strand at 3754.331 kb on - strand at 3754.360 kb on - strand at 3754.389 kb on - strand at 3754.571 kb on - strand, within Pf1N1B4_3366 at 3754.609 kb on + strand, within Pf1N1B4_3366 at 3754.609 kb on + strand, within Pf1N1B4_3366 at 3754.696 kb on + strand, within Pf1N1B4_3366 at 3754.696 kb on + strand, within Pf1N1B4_3366 at 3754.696 kb on + strand, within Pf1N1B4_3366 at 3754.697 kb on - strand, within Pf1N1B4_3366 at 3754.697 kb on - strand, within Pf1N1B4_3366 at 3754.720 kb on + strand, within Pf1N1B4_3366 at 3754.720 kb on + strand, within Pf1N1B4_3366 at 3754.720 kb on + strand, within Pf1N1B4_3366 at 3754.720 kb on + strand, within Pf1N1B4_3366 at 3754.720 kb on + strand, within Pf1N1B4_3366 at 3754.720 kb on + strand at 3754.721 kb on - strand, within Pf1N1B4_3366 at 3754.852 kb on + strand, within Pf1N1B4_3366 at 3754.853 kb on - strand, within Pf1N1B4_3366 at 3754.853 kb on - strand, within Pf1N1B4_3366 at 3754.894 kb on + strand, within Pf1N1B4_3366 at 3754.894 kb on + strand, within Pf1N1B4_3366 at 3754.895 kb on - strand, within Pf1N1B4_3366 at 3755.040 kb on + strand, within Pf1N1B4_3366 at 3755.040 kb on + strand, within Pf1N1B4_3366 at 3755.040 kb on + strand, within Pf1N1B4_3366 at 3755.041 kb on - strand, within Pf1N1B4_3366 at 3755.041 kb on - strand, within Pf1N1B4_3366 at 3755.051 kb on + strand, within Pf1N1B4_3366 at 3755.051 kb on + strand, within Pf1N1B4_3366 at 3755.051 kb on + strand at 3755.052 kb on - strand at 3755.137 kb on + strand at 3755.198 kb on + strand, within Pf1N1B4_3367 at 3755.198 kb on + strand, within Pf1N1B4_3367 at 3755.274 kb on + strand, within Pf1N1B4_3367 at 3755.295 kb on + strand, within Pf1N1B4_3367 at 3755.384 kb on + strand, within Pf1N1B4_3367 at 3755.384 kb on + strand, within Pf1N1B4_3367 at 3755.385 kb on - strand, within Pf1N1B4_3367 at 3755.385 kb on - strand, within Pf1N1B4_3367 at 3755.409 kb on + strand, within Pf1N1B4_3367 at 3755.410 kb on - strand, within Pf1N1B4_3367 at 3755.580 kb on - strand, within Pf1N1B4_3367 at 3755.688 kb on - strand, within Pf1N1B4_3367 at 3755.851 kb on + strand at 3755.851 kb on + strand at 3755.852 kb on - strand at 3755.852 kb on - strand at 3756.164 kb on + strand, within Pf1N1B4_3368 at 3756.164 kb on + strand, within Pf1N1B4_3368 at 3756.290 kb on + strand, within Pf1N1B4_3368 at 3756.291 kb on - strand, within Pf1N1B4_3368 at 3756.309 kb on + strand, within Pf1N1B4_3368 at 3756.497 kb on + strand, within Pf1N1B4_3368 at 3756.782 kb on + strand at 3756.782 kb on + strand at 3756.782 kb on + strand at 3756.782 kb on + strand at 3756.783 kb on - strand at 3756.783 kb on - strand at 3756.783 kb on - strand at 3756.814 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Lactate (C) remove 3,754,316 + -1.1 3,754,330 + +0.1 3,754,330 + +0.1 3,754,330 + +0.2 3,754,330 + +0.5 3,754,330 + +1.6 3,754,331 - -1.9 3,754,331 - -0.6 3,754,331 - +0.6 3,754,360 - -0.9 3,754,389 - -0.4 3,754,571 - Pf1N1B4_3366 0.26 +0.1 3,754,609 + Pf1N1B4_3366 0.31 -1.3 3,754,609 + Pf1N1B4_3366 0.31 -1.3 3,754,696 + Pf1N1B4_3366 0.43 -2.2 3,754,696 + Pf1N1B4_3366 0.43 -0.0 3,754,696 + Pf1N1B4_3366 0.43 +0.4 3,754,697 - Pf1N1B4_3366 0.43 +0.1 3,754,697 - Pf1N1B4_3366 0.43 -0.9 3,754,720 + Pf1N1B4_3366 0.46 -0.2 3,754,720 + Pf1N1B4_3366 0.46 -1.2 3,754,720 + Pf1N1B4_3366 0.46 -1.4 3,754,720 + Pf1N1B4_3366 0.46 +0.2 3,754,720 + Pf1N1B4_3366 0.46 -0.1 3,754,720 + -0.2 3,754,721 - Pf1N1B4_3366 0.46 -0.4 3,754,852 + Pf1N1B4_3366 0.63 +0.1 3,754,853 - Pf1N1B4_3366 0.63 -1.2 3,754,853 - Pf1N1B4_3366 0.63 -0.7 3,754,894 + Pf1N1B4_3366 0.69 +0.4 3,754,894 + Pf1N1B4_3366 0.69 -1.0 3,754,895 - Pf1N1B4_3366 0.69 +0.5 3,755,040 + Pf1N1B4_3366 0.88 -0.7 3,755,040 + Pf1N1B4_3366 0.88 +0.1 3,755,040 + Pf1N1B4_3366 0.88 -1.2 3,755,041 - Pf1N1B4_3366 0.88 +0.1 3,755,041 - Pf1N1B4_3366 0.88 -1.9 3,755,051 + Pf1N1B4_3366 0.90 -0.7 3,755,051 + Pf1N1B4_3366 0.90 -0.2 3,755,051 + -0.4 3,755,052 - -0.3 3,755,137 + -0.1 3,755,198 + Pf1N1B4_3367 0.10 -1.5 3,755,198 + Pf1N1B4_3367 0.10 -3.0 3,755,274 + Pf1N1B4_3367 0.21 -0.7 3,755,295 + Pf1N1B4_3367 0.24 -0.4 3,755,384 + Pf1N1B4_3367 0.37 +0.2 3,755,384 + Pf1N1B4_3367 0.37 +0.6 3,755,385 - Pf1N1B4_3367 0.37 -0.4 3,755,385 - Pf1N1B4_3367 0.37 -0.2 3,755,409 + Pf1N1B4_3367 0.40 -0.4 3,755,410 - Pf1N1B4_3367 0.41 -0.4 3,755,580 - Pf1N1B4_3367 0.65 +0.2 3,755,688 - Pf1N1B4_3367 0.80 -1.4 3,755,851 + -0.4 3,755,851 + -1.4 3,755,852 - -1.2 3,755,852 - -0.6 3,756,164 + Pf1N1B4_3368 0.37 -0.4 3,756,164 + Pf1N1B4_3368 0.37 -0.4 3,756,290 + Pf1N1B4_3368 0.50 -0.4 3,756,291 - Pf1N1B4_3368 0.50 -2.4 3,756,309 + Pf1N1B4_3368 0.52 -0.8 3,756,497 + Pf1N1B4_3368 0.72 -1.2 3,756,782 + +0.5 3,756,782 + +0.2 3,756,782 + -0.0 3,756,782 + +0.6 3,756,783 - +0.4 3,756,783 - -1.4 3,756,783 - -0.9 3,756,814 + -2.0
Or see this region's nucleotide sequence