Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_3367

Experiment: D-Lactate (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_3365 and Pf1N1B4_3366 are separated by 78 nucleotidesPf1N1B4_3366 and Pf1N1B4_3367 overlap by 4 nucleotidesPf1N1B4_3367 and Pf1N1B4_3368 overlap by 4 nucleotides Pf1N1B4_3365 - Sialic acid transporter (permease) NanT, at 3,753,013 to 3,754,296 _3365 Pf1N1B4_3366 - Lactam utilization protein LamB, at 3,754,375 to 3,755,127 _3366 Pf1N1B4_3367 - Allophanate hydrolase 2 subunit 1 (EC 3.5.1.54), at 3,755,124 to 3,755,828 _3367 Pf1N1B4_3368 - Allophanate hydrolase 2 subunit 2 (EC 3.5.1.54), at 3,755,825 to 3,756,751 _3368 Position (kb) 3755 3756Strain fitness (log2 ratio) -3 -2 -1 0 1at 3754.316 kb on + strandat 3754.330 kb on + strandat 3754.330 kb on + strandat 3754.330 kb on + strandat 3754.330 kb on + strandat 3754.330 kb on + strandat 3754.331 kb on - strandat 3754.331 kb on - strandat 3754.331 kb on - strandat 3754.360 kb on - strandat 3754.389 kb on - strandat 3754.571 kb on - strand, within Pf1N1B4_3366at 3754.609 kb on + strand, within Pf1N1B4_3366at 3754.609 kb on + strand, within Pf1N1B4_3366at 3754.696 kb on + strand, within Pf1N1B4_3366at 3754.696 kb on + strand, within Pf1N1B4_3366at 3754.696 kb on + strand, within Pf1N1B4_3366at 3754.697 kb on - strand, within Pf1N1B4_3366at 3754.697 kb on - strand, within Pf1N1B4_3366at 3754.720 kb on + strand, within Pf1N1B4_3366at 3754.720 kb on + strand, within Pf1N1B4_3366at 3754.720 kb on + strand, within Pf1N1B4_3366at 3754.720 kb on + strand, within Pf1N1B4_3366at 3754.720 kb on + strand, within Pf1N1B4_3366at 3754.720 kb on + strandat 3754.721 kb on - strand, within Pf1N1B4_3366at 3754.852 kb on + strand, within Pf1N1B4_3366at 3754.853 kb on - strand, within Pf1N1B4_3366at 3754.853 kb on - strand, within Pf1N1B4_3366at 3754.894 kb on + strand, within Pf1N1B4_3366at 3754.894 kb on + strand, within Pf1N1B4_3366at 3754.895 kb on - strand, within Pf1N1B4_3366at 3755.040 kb on + strand, within Pf1N1B4_3366at 3755.040 kb on + strand, within Pf1N1B4_3366at 3755.040 kb on + strand, within Pf1N1B4_3366at 3755.041 kb on - strand, within Pf1N1B4_3366at 3755.041 kb on - strand, within Pf1N1B4_3366at 3755.051 kb on + strand, within Pf1N1B4_3366at 3755.051 kb on + strand, within Pf1N1B4_3366at 3755.051 kb on + strandat 3755.052 kb on - strandat 3755.137 kb on + strandat 3755.198 kb on + strand, within Pf1N1B4_3367at 3755.198 kb on + strand, within Pf1N1B4_3367at 3755.274 kb on + strand, within Pf1N1B4_3367at 3755.295 kb on + strand, within Pf1N1B4_3367at 3755.384 kb on + strand, within Pf1N1B4_3367at 3755.384 kb on + strand, within Pf1N1B4_3367at 3755.385 kb on - strand, within Pf1N1B4_3367at 3755.385 kb on - strand, within Pf1N1B4_3367at 3755.409 kb on + strand, within Pf1N1B4_3367at 3755.410 kb on - strand, within Pf1N1B4_3367at 3755.580 kb on - strand, within Pf1N1B4_3367at 3755.688 kb on - strand, within Pf1N1B4_3367at 3755.851 kb on + strandat 3755.851 kb on + strandat 3755.852 kb on - strandat 3755.852 kb on - strandat 3756.164 kb on + strand, within Pf1N1B4_3368at 3756.164 kb on + strand, within Pf1N1B4_3368at 3756.290 kb on + strand, within Pf1N1B4_3368at 3756.291 kb on - strand, within Pf1N1B4_3368at 3756.309 kb on + strand, within Pf1N1B4_3368at 3756.497 kb on + strand, within Pf1N1B4_3368at 3756.782 kb on + strandat 3756.782 kb on + strandat 3756.782 kb on + strandat 3756.782 kb on + strandat 3756.783 kb on - strandat 3756.783 kb on - strandat 3756.783 kb on - strandat 3756.814 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Lactate (C)
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3,754,316 + -1.1
3,754,330 + +0.1
3,754,330 + +0.1
3,754,330 + +0.2
3,754,330 + +0.5
3,754,330 + +1.6
3,754,331 - -1.9
3,754,331 - -0.6
3,754,331 - +0.6
3,754,360 - -0.9
3,754,389 - -0.4
3,754,571 - Pf1N1B4_3366 0.26 +0.1
3,754,609 + Pf1N1B4_3366 0.31 -1.3
3,754,609 + Pf1N1B4_3366 0.31 -1.3
3,754,696 + Pf1N1B4_3366 0.43 -2.2
3,754,696 + Pf1N1B4_3366 0.43 -0.0
3,754,696 + Pf1N1B4_3366 0.43 +0.4
3,754,697 - Pf1N1B4_3366 0.43 +0.1
3,754,697 - Pf1N1B4_3366 0.43 -0.9
3,754,720 + Pf1N1B4_3366 0.46 -0.2
3,754,720 + Pf1N1B4_3366 0.46 -1.2
3,754,720 + Pf1N1B4_3366 0.46 -1.4
3,754,720 + Pf1N1B4_3366 0.46 +0.2
3,754,720 + Pf1N1B4_3366 0.46 -0.1
3,754,720 + -0.2
3,754,721 - Pf1N1B4_3366 0.46 -0.4
3,754,852 + Pf1N1B4_3366 0.63 +0.1
3,754,853 - Pf1N1B4_3366 0.63 -1.2
3,754,853 - Pf1N1B4_3366 0.63 -0.7
3,754,894 + Pf1N1B4_3366 0.69 +0.4
3,754,894 + Pf1N1B4_3366 0.69 -1.0
3,754,895 - Pf1N1B4_3366 0.69 +0.5
3,755,040 + Pf1N1B4_3366 0.88 -0.7
3,755,040 + Pf1N1B4_3366 0.88 +0.1
3,755,040 + Pf1N1B4_3366 0.88 -1.2
3,755,041 - Pf1N1B4_3366 0.88 +0.1
3,755,041 - Pf1N1B4_3366 0.88 -1.9
3,755,051 + Pf1N1B4_3366 0.90 -0.7
3,755,051 + Pf1N1B4_3366 0.90 -0.2
3,755,051 + -0.4
3,755,052 - -0.3
3,755,137 + -0.1
3,755,198 + Pf1N1B4_3367 0.10 -1.5
3,755,198 + Pf1N1B4_3367 0.10 -3.0
3,755,274 + Pf1N1B4_3367 0.21 -0.7
3,755,295 + Pf1N1B4_3367 0.24 -0.4
3,755,384 + Pf1N1B4_3367 0.37 +0.2
3,755,384 + Pf1N1B4_3367 0.37 +0.6
3,755,385 - Pf1N1B4_3367 0.37 -0.4
3,755,385 - Pf1N1B4_3367 0.37 -0.2
3,755,409 + Pf1N1B4_3367 0.40 -0.4
3,755,410 - Pf1N1B4_3367 0.41 -0.4
3,755,580 - Pf1N1B4_3367 0.65 +0.2
3,755,688 - Pf1N1B4_3367 0.80 -1.4
3,755,851 + -0.4
3,755,851 + -1.4
3,755,852 - -1.2
3,755,852 - -0.6
3,756,164 + Pf1N1B4_3368 0.37 -0.4
3,756,164 + Pf1N1B4_3368 0.37 -0.4
3,756,290 + Pf1N1B4_3368 0.50 -0.4
3,756,291 - Pf1N1B4_3368 0.50 -2.4
3,756,309 + Pf1N1B4_3368 0.52 -0.8
3,756,497 + Pf1N1B4_3368 0.72 -1.2
3,756,782 + +0.5
3,756,782 + +0.2
3,756,782 + -0.0
3,756,782 + +0.6
3,756,783 - +0.4
3,756,783 - -1.4
3,756,783 - -0.9
3,756,814 + -2.0

Or see this region's nucleotide sequence