Experiment: D-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3313 and Pf1N1B4_3314 are separated by 38 nucleotides Pf1N1B4_3314 and Pf1N1B4_3315 are separated by 18 nucleotides Pf1N1B4_3315 and Pf1N1B4_3316 are separated by 50 nucleotides Pf1N1B4_3316 and Pf1N1B4_3317 overlap by 8 nucleotides
Pf1N1B4_3313 - Tricarboxylate transport protein TctC, at 3,701,994 to 3,702,971
_3313
Pf1N1B4_3314 - Tricarboxylate porin OpdH, at 3,703,010 to 3,704,302
_3314
Pf1N1B4_3315 - hypothetical protein, at 3,704,321 to 3,704,473
_3315
Pf1N1B4_3316 - Tricarboxylate transport transcriptional regulator TctD, at 3,704,524 to 3,705,195
_3316
Pf1N1B4_3317 - Tricarboxylate transport sensor protein TctE, at 3,705,188 to 3,706,576
_3317
Position (kb)
3703
3704
3705 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3702.031 kb on + strand at 3702.031 kb on + strand at 3702.032 kb on - strand at 3702.055 kb on + strand at 3702.055 kb on + strand at 3702.055 kb on + strand at 3702.055 kb on + strand at 3702.055 kb on + strand at 3702.056 kb on - strand at 3702.056 kb on - strand at 3702.056 kb on - strand at 3702.056 kb on - strand at 3702.056 kb on - strand at 3702.169 kb on + strand, within Pf1N1B4_3313 at 3702.199 kb on + strand, within Pf1N1B4_3313 at 3702.199 kb on + strand, within Pf1N1B4_3313 at 3702.200 kb on - strand, within Pf1N1B4_3313 at 3702.232 kb on + strand, within Pf1N1B4_3313 at 3702.425 kb on - strand, within Pf1N1B4_3313 at 3702.425 kb on - strand, within Pf1N1B4_3313 at 3702.425 kb on - strand, within Pf1N1B4_3313 at 3702.425 kb on - strand, within Pf1N1B4_3313 at 3702.572 kb on - strand, within Pf1N1B4_3313 at 3702.572 kb on - strand, within Pf1N1B4_3313 at 3702.604 kb on + strand, within Pf1N1B4_3313 at 3702.605 kb on - strand, within Pf1N1B4_3313 at 3702.605 kb on - strand, within Pf1N1B4_3313 at 3702.749 kb on - strand, within Pf1N1B4_3313 at 3702.778 kb on + strand, within Pf1N1B4_3313 at 3702.778 kb on + strand, within Pf1N1B4_3313 at 3702.778 kb on + strand, within Pf1N1B4_3313 at 3702.778 kb on + strand at 3702.778 kb on + strand, within Pf1N1B4_3313 at 3702.779 kb on - strand, within Pf1N1B4_3313 at 3702.779 kb on - strand, within Pf1N1B4_3313 at 3702.779 kb on - strand, within Pf1N1B4_3313 at 3702.779 kb on - strand, within Pf1N1B4_3313 at 3702.779 kb on - strand, within Pf1N1B4_3313 at 3702.779 kb on - strand, within Pf1N1B4_3313 at 3702.953 kb on + strand at 3702.954 kb on - strand at 3703.003 kb on + strand at 3703.004 kb on - strand at 3703.011 kb on + strand at 3703.029 kb on + strand at 3703.029 kb on + strand at 3703.029 kb on + strand at 3703.029 kb on + strand at 3703.029 kb on + strand at 3703.029 kb on + strand at 3703.029 kb on + strand at 3703.030 kb on - strand at 3703.030 kb on - strand at 3703.030 kb on - strand at 3703.030 kb on - strand at 3703.030 kb on - strand at 3703.030 kb on - strand at 3703.030 kb on - strand at 3703.030 kb on - strand at 3703.030 kb on - strand at 3703.030 kb on - strand at 3703.030 kb on - strand at 3703.030 kb on - strand at 3703.046 kb on + strand at 3703.046 kb on + strand at 3703.046 kb on + strand at 3703.046 kb on + strand at 3703.046 kb on + strand at 3703.047 kb on - strand at 3703.047 kb on - strand at 3703.071 kb on + strand at 3703.071 kb on + strand at 3703.071 kb on + strand at 3703.071 kb on + strand at 3703.072 kb on - strand at 3703.072 kb on - strand at 3703.072 kb on - strand at 3703.072 kb on - strand at 3703.072 kb on - strand at 3703.072 kb on - strand at 3703.083 kb on + strand at 3703.083 kb on + strand at 3703.084 kb on - strand at 3703.084 kb on - strand at 3703.094 kb on - strand at 3703.133 kb on + strand at 3703.133 kb on + strand at 3703.133 kb on + strand at 3703.133 kb on + strand at 3703.140 kb on + strand, within Pf1N1B4_3314 at 3703.140 kb on + strand, within Pf1N1B4_3314 at 3703.140 kb on + strand, within Pf1N1B4_3314 at 3703.140 kb on + strand, within Pf1N1B4_3314 at 3703.141 kb on - strand, within Pf1N1B4_3314 at 3703.141 kb on - strand, within Pf1N1B4_3314 at 3703.141 kb on - strand, within Pf1N1B4_3314 at 3703.141 kb on - strand, within Pf1N1B4_3314 at 3703.263 kb on + strand, within Pf1N1B4_3314 at 3703.264 kb on - strand, within Pf1N1B4_3314 at 3703.335 kb on + strand, within Pf1N1B4_3314 at 3703.335 kb on + strand, within Pf1N1B4_3314 at 3703.357 kb on - strand, within Pf1N1B4_3314 at 3703.405 kb on + strand, within Pf1N1B4_3314 at 3703.405 kb on + strand, within Pf1N1B4_3314 at 3703.406 kb on - strand, within Pf1N1B4_3314 at 3703.406 kb on - strand, within Pf1N1B4_3314 at 3703.422 kb on + strand, within Pf1N1B4_3314 at 3703.422 kb on + strand, within Pf1N1B4_3314 at 3703.422 kb on + strand, within Pf1N1B4_3314 at 3703.422 kb on + strand, within Pf1N1B4_3314 at 3703.422 kb on + strand, within Pf1N1B4_3314 at 3703.422 kb on + strand, within Pf1N1B4_3314 at 3703.569 kb on + strand, within Pf1N1B4_3314 at 3703.569 kb on + strand, within Pf1N1B4_3314 at 3703.569 kb on + strand, within Pf1N1B4_3314 at 3703.569 kb on + strand at 3703.569 kb on + strand, within Pf1N1B4_3314 at 3703.570 kb on - strand, within Pf1N1B4_3314 at 3703.626 kb on + strand, within Pf1N1B4_3314 at 3703.923 kb on + strand, within Pf1N1B4_3314 at 3704.001 kb on - strand, within Pf1N1B4_3314 at 3704.145 kb on + strand, within Pf1N1B4_3314 at 3704.145 kb on + strand, within Pf1N1B4_3314 at 3704.145 kb on + strand, within Pf1N1B4_3314 at 3704.146 kb on - strand, within Pf1N1B4_3314 at 3704.146 kb on - strand, within Pf1N1B4_3314 at 3704.146 kb on - strand, within Pf1N1B4_3314 at 3704.146 kb on - strand, within Pf1N1B4_3314 at 3704.146 kb on - strand, within Pf1N1B4_3314 at 3704.174 kb on - strand at 3704.310 kb on + strand at 3704.310 kb on + strand at 3704.319 kb on + strand at 3704.515 kb on + strand at 3704.515 kb on + strand at 3704.516 kb on - strand at 3704.656 kb on + strand, within Pf1N1B4_3316 at 3704.924 kb on - strand, within Pf1N1B4_3316 at 3705.100 kb on + strand, within Pf1N1B4_3316 at 3705.100 kb on + strand, within Pf1N1B4_3316 at 3705.101 kb on - strand, within Pf1N1B4_3316 at 3705.101 kb on - strand, within Pf1N1B4_3316 at 3705.101 kb on - strand, within Pf1N1B4_3316 at 3705.101 kb on - strand, within Pf1N1B4_3316 at 3705.101 kb on - strand, within Pf1N1B4_3316 at 3705.101 kb on - strand, within Pf1N1B4_3316 at 3705.101 kb on - strand, within Pf1N1B4_3316 at 3705.101 kb on - strand, within Pf1N1B4_3316 at 3705.170 kb on - strand at 3705.194 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Lactate (C) remove 3,702,031 + -1.4 3,702,031 + +0.8 3,702,032 - -0.1 3,702,055 + -1.2 3,702,055 + -0.8 3,702,055 + -0.2 3,702,055 + -0.4 3,702,055 + +0.9 3,702,056 - +0.2 3,702,056 - -1.0 3,702,056 - -1.8 3,702,056 - -2.5 3,702,056 - -0.1 3,702,169 + Pf1N1B4_3313 0.18 -1.0 3,702,199 + Pf1N1B4_3313 0.21 -0.4 3,702,199 + Pf1N1B4_3313 0.21 +0.7 3,702,200 - Pf1N1B4_3313 0.21 -0.7 3,702,232 + Pf1N1B4_3313 0.24 +0.8 3,702,425 - Pf1N1B4_3313 0.44 -0.9 3,702,425 - Pf1N1B4_3313 0.44 -0.1 3,702,425 - Pf1N1B4_3313 0.44 +1.4 3,702,425 - Pf1N1B4_3313 0.44 +0.7 3,702,572 - Pf1N1B4_3313 0.59 -0.6 3,702,572 - Pf1N1B4_3313 0.59 -0.4 3,702,604 + Pf1N1B4_3313 0.62 +0.2 3,702,605 - Pf1N1B4_3313 0.62 +0.0 3,702,605 - Pf1N1B4_3313 0.62 -0.4 3,702,749 - Pf1N1B4_3313 0.77 +0.6 3,702,778 + Pf1N1B4_3313 0.80 +1.2 3,702,778 + Pf1N1B4_3313 0.80 +1.9 3,702,778 + Pf1N1B4_3313 0.80 +0.2 3,702,778 + +0.6 3,702,778 + Pf1N1B4_3313 0.80 -0.1 3,702,779 - Pf1N1B4_3313 0.80 +0.8 3,702,779 - Pf1N1B4_3313 0.80 +0.2 3,702,779 - Pf1N1B4_3313 0.80 -0.3 3,702,779 - Pf1N1B4_3313 0.80 +0.8 3,702,779 - Pf1N1B4_3313 0.80 -0.9 3,702,779 - Pf1N1B4_3313 0.80 +0.2 3,702,953 + +0.8 3,702,954 - -0.1 3,703,003 + +3.6 3,703,004 - -0.0 3,703,011 + +1.6 3,703,029 + +0.7 3,703,029 + +2.5 3,703,029 + +2.6 3,703,029 + +1.2 3,703,029 + +2.4 3,703,029 + +1.9 3,703,029 + +1.1 3,703,030 - +0.4 3,703,030 - -0.6 3,703,030 - +1.8 3,703,030 - +0.2 3,703,030 - +0.7 3,703,030 - -0.3 3,703,030 - -0.7 3,703,030 - +0.2 3,703,030 - -0.1 3,703,030 - -1.8 3,703,030 - +0.3 3,703,030 - +0.2 3,703,046 + -0.2 3,703,046 + +2.4 3,703,046 + +2.0 3,703,046 + +0.9 3,703,046 + +2.5 3,703,047 - +0.5 3,703,047 - +0.6 3,703,071 + +2.0 3,703,071 + +2.3 3,703,071 + +1.5 3,703,071 + +1.4 3,703,072 - +0.2 3,703,072 - +1.2 3,703,072 - +1.2 3,703,072 - -0.2 3,703,072 - +0.6 3,703,072 - -0.5 3,703,083 + +2.7 3,703,083 + +2.6 3,703,084 - -0.7 3,703,084 - -0.4 3,703,094 - -0.2 3,703,133 + -0.3 3,703,133 + +1.7 3,703,133 + +2.3 3,703,133 + +1.2 3,703,140 + Pf1N1B4_3314 0.10 +1.8 3,703,140 + Pf1N1B4_3314 0.10 +1.7 3,703,140 + Pf1N1B4_3314 0.10 +0.7 3,703,140 + Pf1N1B4_3314 0.10 +1.7 3,703,141 - Pf1N1B4_3314 0.10 +0.0 3,703,141 - Pf1N1B4_3314 0.10 -0.7 3,703,141 - Pf1N1B4_3314 0.10 -1.0 3,703,141 - Pf1N1B4_3314 0.10 +0.2 3,703,263 + Pf1N1B4_3314 0.20 -1.2 3,703,264 - Pf1N1B4_3314 0.20 -0.6 3,703,335 + Pf1N1B4_3314 0.25 +1.2 3,703,335 + Pf1N1B4_3314 0.25 -0.2 3,703,357 - Pf1N1B4_3314 0.27 -1.4 3,703,405 + Pf1N1B4_3314 0.31 +0.2 3,703,405 + Pf1N1B4_3314 0.31 +0.2 3,703,406 - Pf1N1B4_3314 0.31 +0.0 3,703,406 - Pf1N1B4_3314 0.31 +1.9 3,703,422 + Pf1N1B4_3314 0.32 +1.2 3,703,422 + Pf1N1B4_3314 0.32 +0.9 3,703,422 + Pf1N1B4_3314 0.32 +1.0 3,703,422 + Pf1N1B4_3314 0.32 +0.5 3,703,422 + Pf1N1B4_3314 0.32 +0.7 3,703,422 + Pf1N1B4_3314 0.32 +0.7 3,703,569 + Pf1N1B4_3314 0.43 +2.0 3,703,569 + Pf1N1B4_3314 0.43 +1.3 3,703,569 + Pf1N1B4_3314 0.43 +1.9 3,703,569 + +0.9 3,703,569 + Pf1N1B4_3314 0.43 +2.5 3,703,570 - Pf1N1B4_3314 0.43 -1.4 3,703,626 + Pf1N1B4_3314 0.48 +0.9 3,703,923 + Pf1N1B4_3314 0.71 +2.2 3,704,001 - Pf1N1B4_3314 0.77 -0.3 3,704,145 + Pf1N1B4_3314 0.88 -0.1 3,704,145 + Pf1N1B4_3314 0.88 +1.6 3,704,145 + Pf1N1B4_3314 0.88 +0.4 3,704,146 - Pf1N1B4_3314 0.88 +1.4 3,704,146 - Pf1N1B4_3314 0.88 +2.4 3,704,146 - Pf1N1B4_3314 0.88 +1.2 3,704,146 - Pf1N1B4_3314 0.88 +1.6 3,704,146 - Pf1N1B4_3314 0.88 +2.3 3,704,174 - -0.7 3,704,310 + +1.2 3,704,310 + +1.2 3,704,319 + +1.8 3,704,515 + +0.0 3,704,515 + -1.6 3,704,516 - +1.9 3,704,656 + Pf1N1B4_3316 0.20 +0.6 3,704,924 - Pf1N1B4_3316 0.60 +0.7 3,705,100 + Pf1N1B4_3316 0.86 +0.9 3,705,100 + Pf1N1B4_3316 0.86 +0.9 3,705,101 - Pf1N1B4_3316 0.86 +0.2 3,705,101 - Pf1N1B4_3316 0.86 +0.4 3,705,101 - Pf1N1B4_3316 0.86 +1.2 3,705,101 - Pf1N1B4_3316 0.86 +0.2 3,705,101 - Pf1N1B4_3316 0.86 +1.6 3,705,101 - Pf1N1B4_3316 0.86 -1.2 3,705,101 - Pf1N1B4_3316 0.86 +1.5 3,705,101 - Pf1N1B4_3316 0.86 -0.5 3,705,170 - +1.2 3,705,194 - -1.6
Or see this region's nucleotide sequence