Experiment: D-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1544 and Pf1N1B4_1545 are separated by 251 nucleotides Pf1N1B4_1545 and Pf1N1B4_1546 are separated by 183 nucleotides Pf1N1B4_1546 and Pf1N1B4_1547 are separated by 13 nucleotides
Pf1N1B4_1544 - Multimodular transpeptidase-transglycosylase (EC 2.4.1.129) (EC 3.4.-.-), at 1,779,960 to 1,782,407
_1544
Pf1N1B4_1545 - NADP-dependent malic enzyme (EC 1.1.1.40), at 1,782,659 to 1,783,927
_1545
Pf1N1B4_1546 - Thermonuclease family protein, at 1,784,111 to 1,784,911
_1546
Pf1N1B4_1547 - LSU ribosomal protein L31p @ LSU ribosomal protein L31p, zinc-dependent, at 1,784,925 to 1,785,155
_1547
Position (kb)
1782
1783
1784 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1781.706 kb on + strand, within Pf1N1B4_1544 at 1781.721 kb on + strand, within Pf1N1B4_1544 at 1781.762 kb on - strand at 1781.762 kb on - strand, within Pf1N1B4_1544 at 1781.934 kb on + strand, within Pf1N1B4_1544 at 1781.934 kb on + strand, within Pf1N1B4_1544 at 1781.934 kb on + strand, within Pf1N1B4_1544 at 1781.934 kb on + strand, within Pf1N1B4_1544 at 1781.934 kb on + strand, within Pf1N1B4_1544 at 1781.934 kb on + strand, within Pf1N1B4_1544 at 1781.934 kb on + strand, within Pf1N1B4_1544 at 1781.934 kb on + strand, within Pf1N1B4_1544 at 1781.934 kb on + strand, within Pf1N1B4_1544 at 1781.935 kb on - strand, within Pf1N1B4_1544 at 1781.935 kb on - strand, within Pf1N1B4_1544 at 1781.935 kb on - strand, within Pf1N1B4_1544 at 1781.935 kb on - strand at 1781.935 kb on - strand, within Pf1N1B4_1544 at 1781.935 kb on - strand, within Pf1N1B4_1544 at 1781.935 kb on - strand, within Pf1N1B4_1544 at 1781.935 kb on - strand, within Pf1N1B4_1544 at 1781.935 kb on - strand, within Pf1N1B4_1544 at 1781.935 kb on - strand, within Pf1N1B4_1544 at 1781.935 kb on - strand, within Pf1N1B4_1544 at 1782.066 kb on + strand, within Pf1N1B4_1544 at 1782.079 kb on - strand, within Pf1N1B4_1544 at 1782.079 kb on - strand, within Pf1N1B4_1544 at 1782.171 kb on + strand at 1782.171 kb on + strand at 1782.172 kb on - strand at 1782.172 kb on - strand at 1782.172 kb on - strand at 1782.172 kb on - strand at 1782.291 kb on + strand at 1782.413 kb on + strand at 1782.413 kb on + strand at 1782.413 kb on + strand at 1782.413 kb on + strand at 1782.413 kb on + strand at 1782.414 kb on - strand at 1782.419 kb on + strand at 1782.419 kb on + strand at 1782.420 kb on - strand at 1782.420 kb on - strand at 1782.423 kb on + strand at 1782.423 kb on + strand at 1782.423 kb on + strand at 1782.424 kb on - strand at 1782.424 kb on - strand at 1782.424 kb on - strand at 1782.424 kb on - strand at 1782.593 kb on + strand at 1782.594 kb on - strand at 1782.696 kb on + strand at 1782.697 kb on - strand at 1782.697 kb on - strand at 1782.697 kb on - strand at 1782.804 kb on + strand, within Pf1N1B4_1545 at 1782.804 kb on + strand, within Pf1N1B4_1545 at 1782.804 kb on + strand, within Pf1N1B4_1545 at 1782.804 kb on + strand, within Pf1N1B4_1545 at 1782.804 kb on + strand, within Pf1N1B4_1545 at 1782.804 kb on + strand, within Pf1N1B4_1545 at 1782.804 kb on + strand, within Pf1N1B4_1545 at 1782.805 kb on - strand, within Pf1N1B4_1545 at 1782.805 kb on - strand, within Pf1N1B4_1545 at 1782.805 kb on - strand, within Pf1N1B4_1545 at 1782.805 kb on - strand, within Pf1N1B4_1545 at 1782.805 kb on - strand, within Pf1N1B4_1545 at 1782.805 kb on - strand, within Pf1N1B4_1545 at 1782.805 kb on - strand, within Pf1N1B4_1545 at 1782.805 kb on - strand, within Pf1N1B4_1545 at 1782.837 kb on + strand, within Pf1N1B4_1545 at 1782.974 kb on + strand, within Pf1N1B4_1545 at 1782.974 kb on + strand, within Pf1N1B4_1545 at 1782.974 kb on + strand, within Pf1N1B4_1545 at 1782.975 kb on - strand, within Pf1N1B4_1545 at 1782.975 kb on - strand, within Pf1N1B4_1545 at 1782.975 kb on - strand, within Pf1N1B4_1545 at 1783.227 kb on + strand, within Pf1N1B4_1545 at 1783.228 kb on - strand, within Pf1N1B4_1545 at 1783.228 kb on - strand, within Pf1N1B4_1545 at 1783.228 kb on - strand, within Pf1N1B4_1545 at 1783.281 kb on + strand, within Pf1N1B4_1545 at 1783.281 kb on + strand, within Pf1N1B4_1545 at 1783.281 kb on + strand, within Pf1N1B4_1545 at 1783.281 kb on + strand, within Pf1N1B4_1545 at 1783.281 kb on + strand, within Pf1N1B4_1545 at 1783.281 kb on + strand at 1783.281 kb on + strand, within Pf1N1B4_1545 at 1783.281 kb on + strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.282 kb on - strand, within Pf1N1B4_1545 at 1783.546 kb on + strand, within Pf1N1B4_1545 at 1783.547 kb on - strand, within Pf1N1B4_1545 at 1783.547 kb on - strand, within Pf1N1B4_1545 at 1783.555 kb on + strand, within Pf1N1B4_1545 at 1783.555 kb on + strand, within Pf1N1B4_1545 at 1783.556 kb on - strand, within Pf1N1B4_1545 at 1783.556 kb on - strand, within Pf1N1B4_1545 at 1783.556 kb on - strand, within Pf1N1B4_1545 at 1783.556 kb on - strand, within Pf1N1B4_1545 at 1783.651 kb on + strand, within Pf1N1B4_1545 at 1783.651 kb on + strand, within Pf1N1B4_1545 at 1783.652 kb on - strand, within Pf1N1B4_1545 at 1783.652 kb on - strand, within Pf1N1B4_1545 at 1783.652 kb on - strand, within Pf1N1B4_1545 at 1783.661 kb on - strand, within Pf1N1B4_1545 at 1783.661 kb on - strand, within Pf1N1B4_1545 at 1783.752 kb on + strand, within Pf1N1B4_1545 at 1783.752 kb on + strand, within Pf1N1B4_1545 at 1783.753 kb on - strand, within Pf1N1B4_1545 at 1783.753 kb on - strand, within Pf1N1B4_1545 at 1783.753 kb on - strand, within Pf1N1B4_1545 at 1783.753 kb on - strand, within Pf1N1B4_1545 at 1783.753 kb on - strand, within Pf1N1B4_1545 at 1783.753 kb on - strand, within Pf1N1B4_1545 at 1783.753 kb on - strand, within Pf1N1B4_1545 at 1783.753 kb on - strand, within Pf1N1B4_1545 at 1783.753 kb on - strand, within Pf1N1B4_1545 at 1783.758 kb on + strand, within Pf1N1B4_1545 at 1783.758 kb on + strand, within Pf1N1B4_1545 at 1783.791 kb on - strand, within Pf1N1B4_1545 at 1783.813 kb on + strand at 1783.815 kb on + strand at 1783.815 kb on + strand at 1783.815 kb on + strand at 1783.816 kb on - strand at 1783.816 kb on - strand at 1783.816 kb on - strand at 1783.816 kb on - strand at 1783.927 kb on + strand at 1783.927 kb on + strand at 1783.927 kb on + strand at 1783.927 kb on + strand at 1783.928 kb on - strand at 1783.928 kb on - strand at 1783.928 kb on - strand at 1783.928 kb on - strand at 1783.945 kb on + strand at 1783.946 kb on - strand at 1783.946 kb on - strand at 1783.973 kb on + strand at 1783.973 kb on + strand at 1783.974 kb on - strand at 1784.294 kb on + strand, within Pf1N1B4_1546 at 1784.294 kb on + strand, within Pf1N1B4_1546 at 1784.295 kb on - strand, within Pf1N1B4_1546 at 1784.562 kb on + strand, within Pf1N1B4_1546 at 1784.562 kb on + strand, within Pf1N1B4_1546 at 1784.562 kb on + strand, within Pf1N1B4_1546 at 1784.562 kb on + strand, within Pf1N1B4_1546 at 1784.562 kb on + strand, within Pf1N1B4_1546 at 1784.562 kb on + strand, within Pf1N1B4_1546 at 1784.562 kb on + strand, within Pf1N1B4_1546 at 1784.563 kb on - strand, within Pf1N1B4_1546 at 1784.563 kb on - strand, within Pf1N1B4_1546 at 1784.563 kb on - strand, within Pf1N1B4_1546 at 1784.563 kb on - strand, within Pf1N1B4_1546 at 1784.563 kb on - strand, within Pf1N1B4_1546 at 1784.563 kb on - strand, within Pf1N1B4_1546 at 1784.719 kb on + strand, within Pf1N1B4_1546
Per-strain Table
Position Strand Gene LocusTag Fraction D-Lactate (C) remove 1,781,706 + Pf1N1B4_1544 0.71 -1.0 1,781,721 + Pf1N1B4_1544 0.72 +0.9 1,781,762 - +1.0 1,781,762 - Pf1N1B4_1544 0.74 +0.3 1,781,934 + Pf1N1B4_1544 0.81 -2.3 1,781,934 + Pf1N1B4_1544 0.81 +0.9 1,781,934 + Pf1N1B4_1544 0.81 -0.0 1,781,934 + Pf1N1B4_1544 0.81 -0.4 1,781,934 + Pf1N1B4_1544 0.81 +1.6 1,781,934 + Pf1N1B4_1544 0.81 +1.0 1,781,934 + Pf1N1B4_1544 0.81 -0.2 1,781,934 + Pf1N1B4_1544 0.81 -0.6 1,781,934 + Pf1N1B4_1544 0.81 +1.6 1,781,935 - Pf1N1B4_1544 0.81 -0.3 1,781,935 - Pf1N1B4_1544 0.81 -1.0 1,781,935 - Pf1N1B4_1544 0.81 +0.6 1,781,935 - +1.6 1,781,935 - Pf1N1B4_1544 0.81 -1.2 1,781,935 - Pf1N1B4_1544 0.81 +1.2 1,781,935 - Pf1N1B4_1544 0.81 +0.1 1,781,935 - Pf1N1B4_1544 0.81 -1.1 1,781,935 - Pf1N1B4_1544 0.81 -0.9 1,781,935 - Pf1N1B4_1544 0.81 -0.9 1,781,935 - Pf1N1B4_1544 0.81 +1.1 1,782,066 + Pf1N1B4_1544 0.86 +0.7 1,782,079 - Pf1N1B4_1544 0.87 +0.0 1,782,079 - Pf1N1B4_1544 0.87 +1.3 1,782,171 + +0.4 1,782,171 + -0.7 1,782,172 - -0.9 1,782,172 - +0.0 1,782,172 - +1.0 1,782,172 - +0.5 1,782,291 + +0.0 1,782,413 + -0.4 1,782,413 + -0.2 1,782,413 + -3.0 1,782,413 + -1.1 1,782,413 + -0.2 1,782,414 - +0.2 1,782,419 + +1.7 1,782,419 + +0.6 1,782,420 - -1.0 1,782,420 - +0.3 1,782,423 + +0.0 1,782,423 + -0.7 1,782,423 + +0.7 1,782,424 - -1.2 1,782,424 - +0.6 1,782,424 - -0.2 1,782,424 - +0.1 1,782,593 + +0.0 1,782,594 - -1.1 1,782,696 + -0.6 1,782,697 - +0.2 1,782,697 - +0.1 1,782,697 - -1.7 1,782,804 + Pf1N1B4_1545 0.11 -1.7 1,782,804 + Pf1N1B4_1545 0.11 -1.7 1,782,804 + Pf1N1B4_1545 0.11 -1.0 1,782,804 + Pf1N1B4_1545 0.11 -1.2 1,782,804 + Pf1N1B4_1545 0.11 +0.8 1,782,804 + Pf1N1B4_1545 0.11 -0.1 1,782,804 + Pf1N1B4_1545 0.11 +1.6 1,782,805 - Pf1N1B4_1545 0.12 -1.0 1,782,805 - Pf1N1B4_1545 0.12 -0.2 1,782,805 - Pf1N1B4_1545 0.12 +0.8 1,782,805 - Pf1N1B4_1545 0.12 -2.7 1,782,805 - Pf1N1B4_1545 0.12 +1.3 1,782,805 - Pf1N1B4_1545 0.12 -0.1 1,782,805 - Pf1N1B4_1545 0.12 -1.3 1,782,805 - Pf1N1B4_1545 0.12 -1.0 1,782,837 + Pf1N1B4_1545 0.14 -0.7 1,782,974 + Pf1N1B4_1545 0.25 +1.2 1,782,974 + Pf1N1B4_1545 0.25 +0.3 1,782,974 + Pf1N1B4_1545 0.25 +0.3 1,782,975 - Pf1N1B4_1545 0.25 +0.6 1,782,975 - Pf1N1B4_1545 0.25 +0.0 1,782,975 - Pf1N1B4_1545 0.25 -2.1 1,783,227 + Pf1N1B4_1545 0.45 -0.4 1,783,228 - Pf1N1B4_1545 0.45 -0.7 1,783,228 - Pf1N1B4_1545 0.45 -1.1 1,783,228 - Pf1N1B4_1545 0.45 -0.9 1,783,281 + Pf1N1B4_1545 0.49 -0.4 1,783,281 + Pf1N1B4_1545 0.49 -1.5 1,783,281 + Pf1N1B4_1545 0.49 -0.4 1,783,281 + Pf1N1B4_1545 0.49 -0.6 1,783,281 + Pf1N1B4_1545 0.49 -0.4 1,783,281 + +0.6 1,783,281 + Pf1N1B4_1545 0.49 -1.0 1,783,281 + Pf1N1B4_1545 0.49 +0.0 1,783,282 - Pf1N1B4_1545 0.49 -0.6 1,783,282 - Pf1N1B4_1545 0.49 -0.1 1,783,282 - Pf1N1B4_1545 0.49 -1.2 1,783,282 - Pf1N1B4_1545 0.49 -0.4 1,783,282 - Pf1N1B4_1545 0.49 +1.0 1,783,282 - Pf1N1B4_1545 0.49 -1.2 1,783,282 - Pf1N1B4_1545 0.49 -2.0 1,783,282 - Pf1N1B4_1545 0.49 -2.1 1,783,282 - Pf1N1B4_1545 0.49 -0.4 1,783,282 - Pf1N1B4_1545 0.49 +0.4 1,783,282 - Pf1N1B4_1545 0.49 -1.2 1,783,282 - Pf1N1B4_1545 0.49 -0.6 1,783,282 - Pf1N1B4_1545 0.49 -1.7 1,783,282 - Pf1N1B4_1545 0.49 -1.2 1,783,282 - Pf1N1B4_1545 0.49 -2.0 1,783,282 - Pf1N1B4_1545 0.49 -0.2 1,783,546 + Pf1N1B4_1545 0.70 +0.6 1,783,547 - Pf1N1B4_1545 0.70 -2.0 1,783,547 - Pf1N1B4_1545 0.70 +1.0 1,783,555 + Pf1N1B4_1545 0.71 -0.3 1,783,555 + Pf1N1B4_1545 0.71 -0.7 1,783,556 - Pf1N1B4_1545 0.71 -1.9 1,783,556 - Pf1N1B4_1545 0.71 +0.3 1,783,556 - Pf1N1B4_1545 0.71 +0.0 1,783,556 - Pf1N1B4_1545 0.71 -0.2 1,783,651 + Pf1N1B4_1545 0.78 +0.9 1,783,651 + Pf1N1B4_1545 0.78 -0.7 1,783,652 - Pf1N1B4_1545 0.78 -0.9 1,783,652 - Pf1N1B4_1545 0.78 -2.3 1,783,652 - Pf1N1B4_1545 0.78 -0.3 1,783,661 - Pf1N1B4_1545 0.79 -0.2 1,783,661 - Pf1N1B4_1545 0.79 -0.2 1,783,752 + Pf1N1B4_1545 0.86 -1.6 1,783,752 + Pf1N1B4_1545 0.86 +1.0 1,783,753 - Pf1N1B4_1545 0.86 -0.6 1,783,753 - Pf1N1B4_1545 0.86 -1.1 1,783,753 - Pf1N1B4_1545 0.86 -1.6 1,783,753 - Pf1N1B4_1545 0.86 -1.9 1,783,753 - Pf1N1B4_1545 0.86 -1.7 1,783,753 - Pf1N1B4_1545 0.86 -1.2 1,783,753 - Pf1N1B4_1545 0.86 +0.0 1,783,753 - Pf1N1B4_1545 0.86 -1.2 1,783,753 - Pf1N1B4_1545 0.86 -1.4 1,783,758 + Pf1N1B4_1545 0.87 -1.0 1,783,758 + Pf1N1B4_1545 0.87 -0.7 1,783,791 - Pf1N1B4_1545 0.89 -1.4 1,783,813 + +0.4 1,783,815 + +0.6 1,783,815 + -0.3 1,783,815 + -2.4 1,783,816 - +0.3 1,783,816 - +0.6 1,783,816 - +1.1 1,783,816 - -2.1 1,783,927 + -0.4 1,783,927 + +0.0 1,783,927 + +0.1 1,783,927 + -1.6 1,783,928 - +0.6 1,783,928 - -1.0 1,783,928 - +0.6 1,783,928 - -1.7 1,783,945 + -1.0 1,783,946 - +0.3 1,783,946 - +0.2 1,783,973 + -1.1 1,783,973 + -0.2 1,783,974 - -0.1 1,784,294 + Pf1N1B4_1546 0.23 -0.4 1,784,294 + Pf1N1B4_1546 0.23 +0.6 1,784,295 - Pf1N1B4_1546 0.23 -1.4 1,784,562 + Pf1N1B4_1546 0.56 -0.4 1,784,562 + Pf1N1B4_1546 0.56 +1.2 1,784,562 + Pf1N1B4_1546 0.56 +0.0 1,784,562 + Pf1N1B4_1546 0.56 +1.6 1,784,562 + Pf1N1B4_1546 0.56 +1.2 1,784,562 + Pf1N1B4_1546 0.56 +1.6 1,784,562 + Pf1N1B4_1546 0.56 -0.4 1,784,563 - Pf1N1B4_1546 0.56 +1.0 1,784,563 - Pf1N1B4_1546 0.56 +1.0 1,784,563 - Pf1N1B4_1546 0.56 -0.5 1,784,563 - Pf1N1B4_1546 0.56 -1.9 1,784,563 - Pf1N1B4_1546 0.56 +0.0 1,784,563 - Pf1N1B4_1546 0.56 +0.2 1,784,719 + Pf1N1B4_1546 0.76 -1.2
Or see this region's nucleotide sequence