Experiment: D-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_127 and Pf1N1B4_128 are separated by 254 nucleotides Pf1N1B4_128 and Pf1N1B4_129 are separated by 3 nucleotides
Pf1N1B4_127 - FIG032225: Transcriptional regulator, LysR family, at 138,939 to 139,847
_127
Pf1N1B4_128 - Methylglyoxal reductase, acetol producing (EC 1.1.1.-) / 2,5-diketo-D-gluconic acid reductase B (EC 1.1.1.274), at 140,102 to 140,905
_128
Pf1N1B4_129 - sensory box histidine kinase/response regulator, at 140,909 to 144,151
_129
Position (kb)
140
141 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 139.134 kb on + strand, within Pf1N1B4_127 at 139.134 kb on + strand, within Pf1N1B4_127 at 139.281 kb on + strand, within Pf1N1B4_127 at 139.281 kb on + strand, within Pf1N1B4_127 at 139.282 kb on - strand, within Pf1N1B4_127 at 139.282 kb on - strand, within Pf1N1B4_127 at 139.282 kb on - strand, within Pf1N1B4_127 at 139.282 kb on - strand, within Pf1N1B4_127 at 139.293 kb on + strand, within Pf1N1B4_127 at 139.293 kb on + strand, within Pf1N1B4_127 at 139.294 kb on - strand, within Pf1N1B4_127 at 139.498 kb on + strand, within Pf1N1B4_127 at 139.542 kb on + strand, within Pf1N1B4_127 at 140.007 kb on + strand at 140.063 kb on + strand at 140.129 kb on - strand at 140.521 kb on + strand, within Pf1N1B4_128 at 140.522 kb on - strand, within Pf1N1B4_128 at 140.574 kb on - strand, within Pf1N1B4_128 at 140.634 kb on - strand, within Pf1N1B4_128 at 140.634 kb on - strand, within Pf1N1B4_128 at 140.634 kb on - strand, within Pf1N1B4_128 at 140.634 kb on - strand, within Pf1N1B4_128 at 140.956 kb on + strand at 140.956 kb on + strand at 140.956 kb on + strand at 140.956 kb on + strand at 140.956 kb on + strand at 140.957 kb on - strand at 140.957 kb on - strand at 140.957 kb on - strand at 141.353 kb on - strand, within Pf1N1B4_129 at 141.353 kb on - strand, within Pf1N1B4_129 at 141.490 kb on + strand, within Pf1N1B4_129
Per-strain Table
Position Strand Gene LocusTag Fraction D-Lactate (C) remove 139,134 + Pf1N1B4_127 0.21 +3.0 139,134 + Pf1N1B4_127 0.21 -0.2 139,281 + Pf1N1B4_127 0.38 +0.4 139,281 + Pf1N1B4_127 0.38 -0.4 139,282 - Pf1N1B4_127 0.38 -0.6 139,282 - Pf1N1B4_127 0.38 -1.2 139,282 - Pf1N1B4_127 0.38 -1.0 139,282 - Pf1N1B4_127 0.38 +0.6 139,293 + Pf1N1B4_127 0.39 +0.2 139,293 + Pf1N1B4_127 0.39 +1.0 139,294 - Pf1N1B4_127 0.39 -1.0 139,498 + Pf1N1B4_127 0.61 +0.7 139,542 + Pf1N1B4_127 0.66 -0.5 140,007 + +1.0 140,063 + -1.0 140,129 - -0.7 140,521 + Pf1N1B4_128 0.52 +1.2 140,522 - Pf1N1B4_128 0.52 +1.0 140,574 - Pf1N1B4_128 0.59 +1.6 140,634 - Pf1N1B4_128 0.66 -0.6 140,634 - Pf1N1B4_128 0.66 -0.0 140,634 - Pf1N1B4_128 0.66 -0.5 140,634 - Pf1N1B4_128 0.66 +2.1 140,956 + +0.0 140,956 + -1.9 140,956 + +1.6 140,956 + -0.6 140,956 + -2.0 140,957 - -1.6 140,957 - +0.4 140,957 - -0.4 141,353 - Pf1N1B4_129 0.14 +1.6 141,353 - Pf1N1B4_129 0.14 +1.2 141,490 + Pf1N1B4_129 0.18 +1.0
Or see this region's nucleotide sequence