Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_121

Experiment: D-Lactate (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_120 and Pf1N1B4_121 are separated by 233 nucleotidesPf1N1B4_121 and Pf1N1B4_122 overlap by 4 nucleotides Pf1N1B4_120 - Channel-forming transporter/cytolysins activator of TpsB family, at 130,211 to 131,938 _120 Pf1N1B4_121 - Membrane fusion component of tripartite multidrug resistance system, at 132,172 to 133,293 _121 Pf1N1B4_122 - Inner membrane component of tripartite multidrug resistance system, at 133,290 to 134,882 _122 Position (kb) 132 133 134Strain fitness (log2 ratio) -1 0 1 2at 131.514 kb on + strand, within Pf1N1B4_120at 131.514 kb on + strand, within Pf1N1B4_120at 131.529 kb on + strandat 131.529 kb on + strand, within Pf1N1B4_120at 131.529 kb on + strand, within Pf1N1B4_120at 131.994 kb on - strandat 132.031 kb on - strandat 132.031 kb on - strandat 132.163 kb on + strandat 132.163 kb on + strandat 132.164 kb on - strandat 132.164 kb on - strandat 132.267 kb on + strandat 132.349 kb on + strand, within Pf1N1B4_121at 132.349 kb on + strand, within Pf1N1B4_121at 132.350 kb on - strand, within Pf1N1B4_121at 132.524 kb on + strand, within Pf1N1B4_121at 132.641 kb on - strand, within Pf1N1B4_121at 132.856 kb on + strand, within Pf1N1B4_121at 132.968 kb on + strand, within Pf1N1B4_121at 133.429 kb on + strandat 133.833 kb on + strand, within Pf1N1B4_122at 133.834 kb on - strand, within Pf1N1B4_122at 133.930 kb on - strand, within Pf1N1B4_122at 133.975 kb on - strand, within Pf1N1B4_122at 134.187 kb on + strand, within Pf1N1B4_122at 134.293 kb on - strand, within Pf1N1B4_122

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Lactate (C)
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131,514 + Pf1N1B4_120 0.75 +0.2
131,514 + Pf1N1B4_120 0.75 +0.7
131,529 + +1.0
131,529 + Pf1N1B4_120 0.76 -0.5
131,529 + Pf1N1B4_120 0.76 -0.9
131,994 - -0.2
132,031 - -0.0
132,031 - +0.6
132,163 + +2.0
132,163 + -0.6
132,164 - +0.1
132,164 - +1.0
132,267 + +0.4
132,349 + Pf1N1B4_121 0.16 +1.6
132,349 + Pf1N1B4_121 0.16 +0.6
132,350 - Pf1N1B4_121 0.16 +0.1
132,524 + Pf1N1B4_121 0.31 +1.6
132,641 - Pf1N1B4_121 0.42 -0.1
132,856 + Pf1N1B4_121 0.61 +1.0
132,968 + Pf1N1B4_121 0.71 -0.2
133,429 + -0.2
133,833 + Pf1N1B4_122 0.34 +0.0
133,834 - Pf1N1B4_122 0.34 +0.4
133,930 - Pf1N1B4_122 0.40 +0.4
133,975 - Pf1N1B4_122 0.43 +1.0
134,187 + Pf1N1B4_122 0.56 +0.3
134,293 - Pf1N1B4_122 0.63 -0.0

Or see this region's nucleotide sequence