Experiment: R2A with Polymyxin B sulfate 0.016 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS21735 and CA265_RS21740 are separated by 113 nucleotides CA265_RS21740 and CA265_RS21745 are separated by 46 nucleotides CA265_RS21745 and CA265_RS21750 are separated by 460 nucleotides
CA265_RS21735: CA265_RS21735 - hypothetical protein, at 5,344,803 to 5,345,744
_RS21735
CA265_RS21740: CA265_RS21740 - RNA polymerase sigma factor, at 5,345,858 to 5,346,409
_RS21740
CA265_RS21745: CA265_RS21745 - hypothetical protein, at 5,346,456 to 5,346,770
_RS21745
CA265_RS21750: CA265_RS21750 - hypothetical protein, at 5,347,231 to 5,347,497
_RS21750
Position (kb) 5345
5346
5347 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5344.874 kb on - strand at 5344.874 kb on - strand at 5344.874 kb on - strand at 5344.874 kb on - strand at 5344.881 kb on - strand at 5344.991 kb on + strand, within CA265_RS21735 at 5345.047 kb on - strand, within CA265_RS21735 at 5345.078 kb on - strand, within CA265_RS21735 at 5345.098 kb on + strand, within CA265_RS21735 at 5345.107 kb on + strand, within CA265_RS21735 at 5345.128 kb on + strand, within CA265_RS21735 at 5345.141 kb on - strand, within CA265_RS21735 at 5345.297 kb on + strand, within CA265_RS21735 at 5345.298 kb on - strand, within CA265_RS21735 at 5345.298 kb on - strand, within CA265_RS21735 at 5345.312 kb on - strand, within CA265_RS21735 at 5345.350 kb on + strand, within CA265_RS21735 at 5345.428 kb on - strand, within CA265_RS21735 at 5345.524 kb on + strand, within CA265_RS21735 at 5345.562 kb on - strand, within CA265_RS21735 at 5345.655 kb on + strand at 5345.656 kb on - strand at 5345.663 kb on - strand at 5345.700 kb on + strand at 5345.701 kb on - strand at 5345.706 kb on - strand at 5345.814 kb on - strand at 5345.909 kb on - strand at 5346.153 kb on + strand, within CA265_RS21740 at 5346.198 kb on + strand, within CA265_RS21740 at 5346.199 kb on - strand, within CA265_RS21740 at 5346.199 kb on - strand, within CA265_RS21740 at 5346.251 kb on + strand, within CA265_RS21740 at 5346.252 kb on - strand, within CA265_RS21740 at 5346.252 kb on - strand, within CA265_RS21740 at 5346.252 kb on - strand, within CA265_RS21740 at 5346.255 kb on + strand, within CA265_RS21740 at 5346.283 kb on + strand, within CA265_RS21740 at 5346.320 kb on - strand, within CA265_RS21740 at 5346.320 kb on - strand, within CA265_RS21740 at 5346.330 kb on + strand, within CA265_RS21740 at 5346.331 kb on - strand, within CA265_RS21740 at 5346.400 kb on + strand at 5346.401 kb on - strand at 5346.404 kb on + strand at 5346.482 kb on + strand at 5346.506 kb on - strand, within CA265_RS21745 at 5346.506 kb on - strand, within CA265_RS21745 at 5346.509 kb on + strand, within CA265_RS21745 at 5346.509 kb on + strand, within CA265_RS21745 at 5346.560 kb on + strand, within CA265_RS21745 at 5346.601 kb on + strand, within CA265_RS21745 at 5346.602 kb on - strand, within CA265_RS21745 at 5346.602 kb on - strand, within CA265_RS21745 at 5346.893 kb on - strand at 5346.893 kb on - strand at 5347.009 kb on + strand at 5347.009 kb on + strand at 5347.010 kb on - strand at 5347.010 kb on - strand at 5347.010 kb on - strand at 5347.013 kb on + strand at 5347.019 kb on - strand at 5347.155 kb on - strand at 5347.208 kb on - strand at 5347.268 kb on + strand, within CA265_RS21750 at 5347.311 kb on + strand, within CA265_RS21750 at 5347.372 kb on + strand, within CA265_RS21750 at 5347.409 kb on - strand, within CA265_RS21750
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.016 mg/ml remove 5,344,874 - +0.0 5,344,874 - +1.7 5,344,874 - +0.7 5,344,874 - -0.5 5,344,881 - +0.2 5,344,991 + CA265_RS21735 0.20 -0.8 5,345,047 - CA265_RS21735 0.26 +0.5 5,345,078 - CA265_RS21735 0.29 -0.2 5,345,098 + CA265_RS21735 0.31 +1.0 5,345,107 + CA265_RS21735 0.32 +0.4 5,345,128 + CA265_RS21735 0.35 +0.3 5,345,141 - CA265_RS21735 0.36 -1.4 5,345,297 + CA265_RS21735 0.52 -0.3 5,345,298 - CA265_RS21735 0.53 -0.0 5,345,298 - CA265_RS21735 0.53 +1.2 5,345,312 - CA265_RS21735 0.54 -0.0 5,345,350 + CA265_RS21735 0.58 +0.0 5,345,428 - CA265_RS21735 0.66 -0.0 5,345,524 + CA265_RS21735 0.77 -0.8 5,345,562 - CA265_RS21735 0.81 -1.3 5,345,655 + -0.6 5,345,656 - +0.8 5,345,663 - -1.7 5,345,700 + +0.4 5,345,701 - +0.3 5,345,706 - -1.0 5,345,814 - -0.5 5,345,909 - -1.0 5,346,153 + CA265_RS21740 0.53 -0.4 5,346,198 + CA265_RS21740 0.62 -0.1 5,346,199 - CA265_RS21740 0.62 +0.2 5,346,199 - CA265_RS21740 0.62 +0.4 5,346,251 + CA265_RS21740 0.71 -1.3 5,346,252 - CA265_RS21740 0.71 +0.5 5,346,252 - CA265_RS21740 0.71 +0.2 5,346,252 - CA265_RS21740 0.71 -0.1 5,346,255 + CA265_RS21740 0.72 -0.5 5,346,283 + CA265_RS21740 0.77 -0.7 5,346,320 - CA265_RS21740 0.84 +0.2 5,346,320 - CA265_RS21740 0.84 +1.1 5,346,330 + CA265_RS21740 0.86 -2.8 5,346,331 - CA265_RS21740 0.86 +0.8 5,346,400 + -0.9 5,346,401 - +0.5 5,346,404 + -0.1 5,346,482 + -2.1 5,346,506 - CA265_RS21745 0.16 -0.9 5,346,506 - CA265_RS21745 0.16 -0.1 5,346,509 + CA265_RS21745 0.17 -1.4 5,346,509 + CA265_RS21745 0.17 -0.4 5,346,560 + CA265_RS21745 0.33 -2.3 5,346,601 + CA265_RS21745 0.46 -2.7 5,346,602 - CA265_RS21745 0.46 -2.1 5,346,602 - CA265_RS21745 0.46 -1.6 5,346,893 - -1.4 5,346,893 - -2.3 5,347,009 + -1.5 5,347,009 + -1.5 5,347,010 - +2.2 5,347,010 - -1.1 5,347,010 - -0.4 5,347,013 + -1.4 5,347,019 - +0.1 5,347,155 - -0.6 5,347,208 - -0.1 5,347,268 + CA265_RS21750 0.14 -0.3 5,347,311 + CA265_RS21750 0.30 -1.5 5,347,372 + CA265_RS21750 0.53 -2.7 5,347,409 - CA265_RS21750 0.67 +0.4
Or see this region's nucleotide sequence