Experiment: R2A with Polymyxin B sulfate 0.016 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS21095 and CA265_RS21100 are separated by 125 nucleotides CA265_RS21100 and CA265_RS21105 are separated by 61 nucleotides CA265_RS21105 and CA265_RS21110 are separated by 78 nucleotides
CA265_RS21095: CA265_RS21095 - 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase, at 5,197,835 to 5,200,168
_RS21095
CA265_RS21100: CA265_RS21100 - acyl-CoA thioesterase, at 5,200,294 to 5,200,680
_RS21100
CA265_RS21105: CA265_RS21105 - 16S rRNA (cytosine(1402)-N(4))-methyltransferase, at 5,200,742 to 5,201,407
_RS21105
CA265_RS21110: CA265_RS21110 - phosphatidate cytidylyltransferase, at 5,201,486 to 5,202,295
_RS21110
Position (kb)
5200
5201 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5199.334 kb on + strand, within CA265_RS21095 at 5199.407 kb on + strand, within CA265_RS21095 at 5199.407 kb on + strand, within CA265_RS21095 at 5199.408 kb on - strand, within CA265_RS21095 at 5199.474 kb on + strand, within CA265_RS21095 at 5199.475 kb on - strand, within CA265_RS21095 at 5199.512 kb on + strand at 5199.529 kb on - strand, within CA265_RS21095 at 5199.549 kb on + strand, within CA265_RS21095 at 5199.550 kb on - strand, within CA265_RS21095 at 5199.550 kb on - strand, within CA265_RS21095 at 5199.651 kb on + strand, within CA265_RS21095 at 5199.651 kb on + strand, within CA265_RS21095 at 5199.722 kb on + strand, within CA265_RS21095 at 5199.723 kb on - strand, within CA265_RS21095 at 5199.739 kb on + strand, within CA265_RS21095 at 5199.739 kb on + strand, within CA265_RS21095 at 5199.803 kb on + strand, within CA265_RS21095 at 5199.913 kb on + strand, within CA265_RS21095 at 5199.945 kb on - strand at 5200.093 kb on - strand at 5200.127 kb on - strand at 5200.167 kb on - strand at 5200.210 kb on + strand at 5200.284 kb on + strand at 5200.285 kb on - strand at 5200.357 kb on - strand, within CA265_RS21100 at 5200.453 kb on - strand, within CA265_RS21100 at 5200.464 kb on - strand, within CA265_RS21100 at 5200.509 kb on + strand, within CA265_RS21100 at 5200.509 kb on + strand, within CA265_RS21100 at 5200.510 kb on - strand, within CA265_RS21100 at 5200.510 kb on - strand, within CA265_RS21100 at 5200.533 kb on - strand, within CA265_RS21100 at 5200.548 kb on + strand, within CA265_RS21100 at 5200.577 kb on - strand, within CA265_RS21100 at 5200.591 kb on + strand, within CA265_RS21100 at 5200.777 kb on + strand at 5200.847 kb on - strand, within CA265_RS21105 at 5200.948 kb on - strand, within CA265_RS21105 at 5200.948 kb on - strand, within CA265_RS21105 at 5201.077 kb on + strand, within CA265_RS21105 at 5201.077 kb on + strand, within CA265_RS21105 at 5201.078 kb on - strand, within CA265_RS21105 at 5201.125 kb on + strand, within CA265_RS21105 at 5201.126 kb on - strand, within CA265_RS21105 at 5201.133 kb on - strand, within CA265_RS21105 at 5201.133 kb on - strand, within CA265_RS21105 at 5201.156 kb on + strand, within CA265_RS21105 at 5201.157 kb on - strand, within CA265_RS21105 at 5201.157 kb on - strand, within CA265_RS21105 at 5201.245 kb on + strand, within CA265_RS21105 at 5201.292 kb on + strand, within CA265_RS21105 at 5201.312 kb on - strand, within CA265_RS21105 at 5201.312 kb on - strand, within CA265_RS21105 at 5201.354 kb on - strand at 5201.381 kb on + strand at 5201.384 kb on + strand at 5201.392 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.016 mg/ml remove 5,199,334 + CA265_RS21095 0.64 -2.0 5,199,407 + CA265_RS21095 0.67 -0.3 5,199,407 + CA265_RS21095 0.67 -1.4 5,199,408 - CA265_RS21095 0.67 -0.9 5,199,474 + CA265_RS21095 0.70 -1.3 5,199,475 - CA265_RS21095 0.70 +1.4 5,199,512 + -0.6 5,199,529 - CA265_RS21095 0.73 +2.1 5,199,549 + CA265_RS21095 0.73 -0.5 5,199,550 - CA265_RS21095 0.73 -0.5 5,199,550 - CA265_RS21095 0.73 -0.9 5,199,651 + CA265_RS21095 0.78 -0.8 5,199,651 + CA265_RS21095 0.78 -0.7 5,199,722 + CA265_RS21095 0.81 +1.0 5,199,723 - CA265_RS21095 0.81 -0.3 5,199,739 + CA265_RS21095 0.82 +2.2 5,199,739 + CA265_RS21095 0.82 -2.0 5,199,803 + CA265_RS21095 0.84 -1.0 5,199,913 + CA265_RS21095 0.89 -2.9 5,199,945 - +0.4 5,200,093 - +0.1 5,200,127 - +0.8 5,200,167 - -0.2 5,200,210 + -2.0 5,200,284 + -0.4 5,200,285 - +0.0 5,200,357 - CA265_RS21100 0.16 -0.6 5,200,453 - CA265_RS21100 0.41 -0.3 5,200,464 - CA265_RS21100 0.44 +0.8 5,200,509 + CA265_RS21100 0.56 -0.4 5,200,509 + CA265_RS21100 0.56 -2.3 5,200,510 - CA265_RS21100 0.56 -0.4 5,200,510 - CA265_RS21100 0.56 -0.9 5,200,533 - CA265_RS21100 0.62 -1.1 5,200,548 + CA265_RS21100 0.66 -0.2 5,200,577 - CA265_RS21100 0.73 -0.9 5,200,591 + CA265_RS21100 0.77 -3.4 5,200,777 + -0.3 5,200,847 - CA265_RS21105 0.16 +0.5 5,200,948 - CA265_RS21105 0.31 +0.9 5,200,948 - CA265_RS21105 0.31 +0.8 5,201,077 + CA265_RS21105 0.50 -0.1 5,201,077 + CA265_RS21105 0.50 -0.9 5,201,078 - CA265_RS21105 0.50 +0.1 5,201,125 + CA265_RS21105 0.58 -0.1 5,201,126 - CA265_RS21105 0.58 -0.8 5,201,133 - CA265_RS21105 0.59 +0.8 5,201,133 - CA265_RS21105 0.59 +0.7 5,201,156 + CA265_RS21105 0.62 -0.8 5,201,157 - CA265_RS21105 0.62 -0.7 5,201,157 - CA265_RS21105 0.62 +0.9 5,201,245 + CA265_RS21105 0.76 -0.8 5,201,292 + CA265_RS21105 0.83 +0.3 5,201,312 - CA265_RS21105 0.86 +0.1 5,201,312 - CA265_RS21105 0.86 +0.4 5,201,354 - +0.4 5,201,381 + +0.0 5,201,384 + +0.6 5,201,392 - +1.6
Or see this region's nucleotide sequence