Experiment: R2A with Polymyxin B sulfate 0.016 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS15645 and CA265_RS15650 are separated by 28 nucleotides CA265_RS15650 and CA265_RS15655 are separated by 64 nucleotides CA265_RS15655 and CA265_RS15660 are separated by 26 nucleotides
CA265_RS15645: CA265_RS15645 - calcium-binding protein, at 3,942,321 to 3,943,625
_RS15645
CA265_RS15650: CA265_RS15650 - hypothetical protein, at 3,943,654 to 3,944,247
_RS15650
CA265_RS15655: CA265_RS15655 - hypothetical protein, at 3,944,312 to 3,945,994
_RS15655
CA265_RS15660: CA265_RS15660 - hypothetical protein, at 3,946,021 to 3,949,323
_RS15660
Position (kb)
3944
3945
3946 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3943.316 kb on + strand, within CA265_RS15645 at 3943.317 kb on - strand, within CA265_RS15645 at 3943.331 kb on + strand, within CA265_RS15645 at 3943.408 kb on + strand, within CA265_RS15645 at 3943.437 kb on - strand, within CA265_RS15645 at 3943.438 kb on + strand, within CA265_RS15645 at 3943.439 kb on - strand, within CA265_RS15645 at 3943.450 kb on - strand, within CA265_RS15645 at 3943.463 kb on + strand, within CA265_RS15645 at 3943.488 kb on + strand, within CA265_RS15645 at 3943.548 kb on - strand at 3943.548 kb on - strand at 3943.567 kb on - strand at 3943.665 kb on + strand at 3943.665 kb on + strand at 3943.666 kb on - strand at 3943.669 kb on - strand at 3943.695 kb on + strand at 3943.696 kb on - strand at 3943.710 kb on + strand at 3943.729 kb on + strand, within CA265_RS15650 at 3943.921 kb on - strand, within CA265_RS15650 at 3943.921 kb on - strand, within CA265_RS15650 at 3943.933 kb on - strand, within CA265_RS15650 at 3943.957 kb on - strand, within CA265_RS15650 at 3944.074 kb on + strand, within CA265_RS15650 at 3944.075 kb on - strand, within CA265_RS15650 at 3944.075 kb on - strand, within CA265_RS15650 at 3944.206 kb on - strand at 3944.230 kb on + strand at 3944.230 kb on + strand at 3944.275 kb on - strand at 3944.306 kb on - strand at 3944.313 kb on + strand at 3944.441 kb on + strand at 3944.453 kb on - strand at 3944.483 kb on - strand, within CA265_RS15655 at 3944.698 kb on + strand, within CA265_RS15655 at 3944.698 kb on + strand, within CA265_RS15655 at 3944.702 kb on + strand, within CA265_RS15655 at 3944.703 kb on - strand, within CA265_RS15655 at 3944.707 kb on + strand, within CA265_RS15655 at 3944.712 kb on + strand, within CA265_RS15655 at 3944.712 kb on + strand, within CA265_RS15655 at 3944.742 kb on + strand, within CA265_RS15655 at 3944.743 kb on - strand, within CA265_RS15655 at 3944.743 kb on - strand, within CA265_RS15655 at 3944.745 kb on + strand, within CA265_RS15655 at 3944.818 kb on - strand, within CA265_RS15655 at 3944.818 kb on - strand, within CA265_RS15655 at 3944.818 kb on - strand, within CA265_RS15655 at 3944.822 kb on + strand, within CA265_RS15655 at 3944.823 kb on - strand, within CA265_RS15655 at 3944.848 kb on + strand, within CA265_RS15655 at 3944.875 kb on + strand, within CA265_RS15655 at 3944.875 kb on + strand, within CA265_RS15655 at 3944.876 kb on - strand, within CA265_RS15655 at 3944.895 kb on - strand, within CA265_RS15655 at 3944.904 kb on - strand, within CA265_RS15655 at 3944.947 kb on + strand, within CA265_RS15655 at 3944.948 kb on - strand, within CA265_RS15655 at 3944.959 kb on + strand, within CA265_RS15655 at 3944.959 kb on + strand, within CA265_RS15655 at 3944.989 kb on - strand, within CA265_RS15655 at 3945.119 kb on - strand, within CA265_RS15655 at 3945.144 kb on + strand, within CA265_RS15655 at 3945.161 kb on - strand, within CA265_RS15655 at 3945.173 kb on - strand, within CA265_RS15655 at 3945.203 kb on + strand, within CA265_RS15655 at 3945.255 kb on - strand, within CA265_RS15655 at 3945.258 kb on - strand, within CA265_RS15655 at 3945.258 kb on - strand, within CA265_RS15655 at 3945.259 kb on + strand, within CA265_RS15655 at 3945.260 kb on - strand, within CA265_RS15655 at 3945.260 kb on - strand, within CA265_RS15655 at 3945.270 kb on - strand, within CA265_RS15655 at 3945.317 kb on + strand, within CA265_RS15655 at 3945.318 kb on - strand, within CA265_RS15655 at 3945.321 kb on - strand, within CA265_RS15655 at 3945.369 kb on + strand, within CA265_RS15655 at 3945.496 kb on + strand, within CA265_RS15655 at 3945.508 kb on + strand, within CA265_RS15655 at 3945.509 kb on - strand, within CA265_RS15655 at 3945.509 kb on - strand, within CA265_RS15655 at 3945.510 kb on + strand, within CA265_RS15655 at 3945.510 kb on + strand, within CA265_RS15655 at 3945.533 kb on - strand, within CA265_RS15655 at 3945.533 kb on - strand, within CA265_RS15655 at 3945.533 kb on - strand, within CA265_RS15655 at 3945.533 kb on - strand, within CA265_RS15655 at 3945.533 kb on - strand, within CA265_RS15655 at 3945.541 kb on + strand, within CA265_RS15655 at 3945.616 kb on - strand, within CA265_RS15655 at 3945.644 kb on - strand, within CA265_RS15655 at 3945.714 kb on - strand, within CA265_RS15655 at 3945.716 kb on - strand, within CA265_RS15655 at 3945.734 kb on - strand, within CA265_RS15655 at 3945.745 kb on + strand, within CA265_RS15655 at 3945.746 kb on - strand, within CA265_RS15655 at 3945.746 kb on - strand, within CA265_RS15655 at 3945.749 kb on - strand, within CA265_RS15655 at 3945.785 kb on + strand, within CA265_RS15655 at 3945.786 kb on - strand, within CA265_RS15655 at 3945.796 kb on - strand, within CA265_RS15655 at 3945.804 kb on + strand, within CA265_RS15655 at 3945.820 kb on + strand, within CA265_RS15655 at 3945.869 kb on + strand at 3945.869 kb on + strand at 3945.898 kb on + strand at 3945.899 kb on - strand at 3945.899 kb on - strand at 3945.899 kb on - strand at 3945.900 kb on + strand at 3945.901 kb on - strand at 3945.930 kb on + strand at 3945.930 kb on + strand at 3945.951 kb on - strand at 3946.123 kb on + strand at 3946.123 kb on + strand at 3946.123 kb on + strand at 3946.123 kb on + strand at 3946.170 kb on + strand at 3946.175 kb on + strand at 3946.207 kb on + strand at 3946.207 kb on + strand at 3946.208 kb on - strand at 3946.229 kb on + strand at 3946.229 kb on + strand at 3946.229 kb on + strand at 3946.230 kb on - strand at 3946.235 kb on - strand at 3946.318 kb on - strand at 3946.319 kb on + strand at 3946.322 kb on + strand at 3946.349 kb on - strand at 3946.402 kb on + strand, within CA265_RS15660 at 3946.405 kb on + strand, within CA265_RS15660 at 3946.406 kb on - strand, within CA265_RS15660 at 3946.423 kb on + strand, within CA265_RS15660 at 3946.424 kb on - strand, within CA265_RS15660 at 3946.470 kb on - strand, within CA265_RS15660 at 3946.470 kb on - strand, within CA265_RS15660 at 3946.477 kb on - strand, within CA265_RS15660 at 3946.562 kb on + strand, within CA265_RS15660 at 3946.563 kb on - strand, within CA265_RS15660 at 3946.615 kb on - strand, within CA265_RS15660 at 3946.627 kb on - strand, within CA265_RS15660 at 3946.632 kb on + strand, within CA265_RS15660 at 3946.637 kb on + strand, within CA265_RS15660 at 3946.665 kb on + strand, within CA265_RS15660 at 3946.665 kb on + strand, within CA265_RS15660 at 3946.692 kb on - strand, within CA265_RS15660 at 3946.695 kb on + strand, within CA265_RS15660 at 3946.707 kb on + strand, within CA265_RS15660 at 3946.799 kb on + strand, within CA265_RS15660 at 3946.814 kb on + strand, within CA265_RS15660 at 3946.830 kb on + strand, within CA265_RS15660 at 3946.831 kb on - strand, within CA265_RS15660 at 3946.831 kb on - strand, within CA265_RS15660 at 3946.863 kb on + strand, within CA265_RS15660 at 3946.864 kb on - strand, within CA265_RS15660 at 3946.888 kb on + strand, within CA265_RS15660 at 3946.889 kb on - strand, within CA265_RS15660 at 3946.929 kb on + strand, within CA265_RS15660 at 3946.944 kb on + strand, within CA265_RS15660 at 3946.977 kb on + strand, within CA265_RS15660 at 3946.980 kb on + strand, within CA265_RS15660 at 3946.980 kb on + strand, within CA265_RS15660 at 3946.981 kb on - strand, within CA265_RS15660
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.016 mg/ml remove 3,943,316 + CA265_RS15645 0.76 +0.6 3,943,317 - CA265_RS15645 0.76 -0.8 3,943,331 + CA265_RS15645 0.77 -0.4 3,943,408 + CA265_RS15645 0.83 +0.1 3,943,437 - CA265_RS15645 0.86 +0.6 3,943,438 + CA265_RS15645 0.86 +0.9 3,943,439 - CA265_RS15645 0.86 +0.8 3,943,450 - CA265_RS15645 0.87 +0.2 3,943,463 + CA265_RS15645 0.88 +0.4 3,943,488 + CA265_RS15645 0.89 +0.6 3,943,548 - +0.6 3,943,548 - -0.6 3,943,567 - -1.5 3,943,665 + +0.4 3,943,665 + -0.0 3,943,666 - -1.6 3,943,669 - +0.2 3,943,695 + +0.9 3,943,696 - +0.6 3,943,710 + +0.1 3,943,729 + CA265_RS15650 0.13 +0.7 3,943,921 - CA265_RS15650 0.45 +1.6 3,943,921 - CA265_RS15650 0.45 +0.4 3,943,933 - CA265_RS15650 0.47 +0.3 3,943,957 - CA265_RS15650 0.51 +1.1 3,944,074 + CA265_RS15650 0.71 -0.0 3,944,075 - CA265_RS15650 0.71 +0.8 3,944,075 - CA265_RS15650 0.71 +0.8 3,944,206 - +0.6 3,944,230 + +0.4 3,944,230 + -0.3 3,944,275 - +1.2 3,944,306 - +0.3 3,944,313 + -0.6 3,944,441 + -0.1 3,944,453 - -1.5 3,944,483 - CA265_RS15655 0.10 -1.5 3,944,698 + CA265_RS15655 0.23 -0.2 3,944,698 + CA265_RS15655 0.23 +1.0 3,944,702 + CA265_RS15655 0.23 -1.3 3,944,703 - CA265_RS15655 0.23 -1.3 3,944,707 + CA265_RS15655 0.23 -0.1 3,944,712 + CA265_RS15655 0.24 -0.2 3,944,712 + CA265_RS15655 0.24 -0.7 3,944,742 + CA265_RS15655 0.26 -0.1 3,944,743 - CA265_RS15655 0.26 +1.1 3,944,743 - CA265_RS15655 0.26 -0.8 3,944,745 + CA265_RS15655 0.26 -0.0 3,944,818 - CA265_RS15655 0.30 -0.8 3,944,818 - CA265_RS15655 0.30 -0.6 3,944,818 - CA265_RS15655 0.30 -0.2 3,944,822 + CA265_RS15655 0.30 -0.1 3,944,823 - CA265_RS15655 0.30 +0.0 3,944,848 + CA265_RS15655 0.32 -0.2 3,944,875 + CA265_RS15655 0.33 -0.0 3,944,875 + CA265_RS15655 0.33 -0.5 3,944,876 - CA265_RS15655 0.34 -0.2 3,944,895 - CA265_RS15655 0.35 +0.1 3,944,904 - CA265_RS15655 0.35 -0.8 3,944,947 + CA265_RS15655 0.38 -0.5 3,944,948 - CA265_RS15655 0.38 +0.1 3,944,959 + CA265_RS15655 0.38 -0.9 3,944,959 + CA265_RS15655 0.38 +0.1 3,944,989 - CA265_RS15655 0.40 -1.6 3,945,119 - CA265_RS15655 0.48 -1.4 3,945,144 + CA265_RS15655 0.49 +0.1 3,945,161 - CA265_RS15655 0.50 -0.2 3,945,173 - CA265_RS15655 0.51 +1.1 3,945,203 + CA265_RS15655 0.53 -0.6 3,945,255 - CA265_RS15655 0.56 -1.2 3,945,258 - CA265_RS15655 0.56 -0.3 3,945,258 - CA265_RS15655 0.56 -1.6 3,945,259 + CA265_RS15655 0.56 -0.7 3,945,260 - CA265_RS15655 0.56 -1.0 3,945,260 - CA265_RS15655 0.56 +0.3 3,945,270 - CA265_RS15655 0.57 -0.3 3,945,317 + CA265_RS15655 0.60 -0.0 3,945,318 - CA265_RS15655 0.60 -0.2 3,945,321 - CA265_RS15655 0.60 -2.3 3,945,369 + CA265_RS15655 0.63 -0.1 3,945,496 + CA265_RS15655 0.70 -0.5 3,945,508 + CA265_RS15655 0.71 -0.4 3,945,509 - CA265_RS15655 0.71 +0.0 3,945,509 - CA265_RS15655 0.71 -1.2 3,945,510 + CA265_RS15655 0.71 +0.3 3,945,510 + CA265_RS15655 0.71 -0.5 3,945,533 - CA265_RS15655 0.73 -0.1 3,945,533 - CA265_RS15655 0.73 -0.3 3,945,533 - CA265_RS15655 0.73 +0.3 3,945,533 - CA265_RS15655 0.73 +0.7 3,945,533 - CA265_RS15655 0.73 -0.5 3,945,541 + CA265_RS15655 0.73 +0.3 3,945,616 - CA265_RS15655 0.77 -0.4 3,945,644 - CA265_RS15655 0.79 +1.8 3,945,714 - CA265_RS15655 0.83 -1.8 3,945,716 - CA265_RS15655 0.83 +0.1 3,945,734 - CA265_RS15655 0.84 -0.2 3,945,745 + CA265_RS15655 0.85 +0.5 3,945,746 - CA265_RS15655 0.85 -0.6 3,945,746 - CA265_RS15655 0.85 -0.7 3,945,749 - CA265_RS15655 0.85 -0.2 3,945,785 + CA265_RS15655 0.88 +0.5 3,945,786 - CA265_RS15655 0.88 -2.5 3,945,796 - CA265_RS15655 0.88 +0.4 3,945,804 + CA265_RS15655 0.89 -0.4 3,945,820 + CA265_RS15655 0.90 -0.5 3,945,869 + +0.3 3,945,869 + -0.3 3,945,898 + -0.5 3,945,899 - -0.9 3,945,899 - -1.5 3,945,899 - +0.1 3,945,900 + -0.1 3,945,901 - -0.2 3,945,930 + -0.8 3,945,930 + +0.1 3,945,951 - +0.3 3,946,123 + -0.3 3,946,123 + +0.1 3,946,123 + +0.6 3,946,123 + +0.9 3,946,170 + +0.8 3,946,175 + +0.4 3,946,207 + -0.3 3,946,207 + +1.5 3,946,208 - +1.1 3,946,229 + +0.0 3,946,229 + -0.3 3,946,229 + -2.5 3,946,230 - +0.9 3,946,235 - -0.4 3,946,318 - -0.1 3,946,319 + -0.7 3,946,322 + -1.8 3,946,349 - -0.4 3,946,402 + CA265_RS15660 0.12 +0.0 3,946,405 + CA265_RS15660 0.12 +0.6 3,946,406 - CA265_RS15660 0.12 +0.4 3,946,423 + CA265_RS15660 0.12 +0.3 3,946,424 - CA265_RS15660 0.12 -0.4 3,946,470 - CA265_RS15660 0.14 -0.7 3,946,470 - CA265_RS15660 0.14 -0.7 3,946,477 - CA265_RS15660 0.14 -0.0 3,946,562 + CA265_RS15660 0.16 -0.1 3,946,563 - CA265_RS15660 0.16 -0.6 3,946,615 - CA265_RS15660 0.18 -0.2 3,946,627 - CA265_RS15660 0.18 -0.6 3,946,632 + CA265_RS15660 0.18 +0.5 3,946,637 + CA265_RS15660 0.19 -1.0 3,946,665 + CA265_RS15660 0.19 +0.8 3,946,665 + CA265_RS15660 0.19 +0.8 3,946,692 - CA265_RS15660 0.20 +1.1 3,946,695 + CA265_RS15660 0.20 +0.3 3,946,707 + CA265_RS15660 0.21 +0.8 3,946,799 + CA265_RS15660 0.24 +0.6 3,946,814 + CA265_RS15660 0.24 +0.8 3,946,830 + CA265_RS15660 0.24 +0.8 3,946,831 - CA265_RS15660 0.25 -0.6 3,946,831 - CA265_RS15660 0.25 -0.2 3,946,863 + CA265_RS15660 0.25 +0.8 3,946,864 - CA265_RS15660 0.26 -0.4 3,946,888 + CA265_RS15660 0.26 +0.4 3,946,889 - CA265_RS15660 0.26 +1.1 3,946,929 + CA265_RS15660 0.27 -0.7 3,946,944 + CA265_RS15660 0.28 +1.9 3,946,977 + CA265_RS15660 0.29 -0.9 3,946,980 + CA265_RS15660 0.29 -0.9 3,946,980 + CA265_RS15660 0.29 +0.6 3,946,981 - CA265_RS15660 0.29 -0.0
Or see this region's nucleotide sequence