Experiment: R2A with Polymyxin B sulfate 0.016 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS13275 and CA265_RS13280 are separated by 362 nucleotides CA265_RS13280 and CA265_RS13285 are separated by 9 nucleotides
CA265_RS13275: CA265_RS13275 - redox-regulated ATPase YchF, at 3,297,218 to 3,298,318
_RS13275
CA265_RS13280: CA265_RS13280 - hydrolase, at 3,298,681 to 3,299,295
_RS13280
CA265_RS13285: CA265_RS13285 - magnesium transporter, at 3,299,305 to 3,300,681
_RS13285
Position (kb)
3298
3299
3300 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3297.704 kb on + strand, within CA265_RS13275 at 3297.704 kb on + strand, within CA265_RS13275 at 3297.745 kb on + strand, within CA265_RS13275 at 3297.812 kb on - strand, within CA265_RS13275 at 3297.830 kb on + strand, within CA265_RS13275 at 3297.831 kb on - strand, within CA265_RS13275 at 3297.886 kb on - strand, within CA265_RS13275 at 3297.967 kb on + strand, within CA265_RS13275 at 3297.972 kb on - strand, within CA265_RS13275 at 3298.049 kb on - strand, within CA265_RS13275 at 3298.071 kb on - strand, within CA265_RS13275 at 3298.215 kb on + strand at 3298.216 kb on - strand at 3298.216 kb on - strand at 3298.217 kb on - strand at 3298.252 kb on - strand at 3298.257 kb on + strand at 3298.258 kb on - strand at 3298.258 kb on - strand at 3298.275 kb on + strand at 3298.430 kb on + strand at 3298.573 kb on + strand at 3298.574 kb on - strand at 3298.583 kb on + strand at 3298.583 kb on + strand at 3298.587 kb on + strand at 3298.587 kb on + strand at 3298.588 kb on - strand at 3298.635 kb on + strand at 3298.635 kb on + strand at 3298.668 kb on + strand at 3298.669 kb on - strand at 3298.675 kb on + strand at 3298.682 kb on + strand at 3298.683 kb on - strand at 3298.698 kb on - strand at 3298.711 kb on + strand at 3298.757 kb on - strand, within CA265_RS13280 at 3298.799 kb on + strand, within CA265_RS13280 at 3298.799 kb on + strand, within CA265_RS13280 at 3298.800 kb on - strand, within CA265_RS13280 at 3298.800 kb on - strand, within CA265_RS13280 at 3298.800 kb on - strand, within CA265_RS13280 at 3298.986 kb on + strand, within CA265_RS13280 at 3298.988 kb on + strand, within CA265_RS13280 at 3298.989 kb on - strand, within CA265_RS13280 at 3298.989 kb on - strand, within CA265_RS13280 at 3299.103 kb on + strand, within CA265_RS13280 at 3299.129 kb on - strand, within CA265_RS13280 at 3299.144 kb on + strand, within CA265_RS13280 at 3299.144 kb on + strand, within CA265_RS13280 at 3299.145 kb on - strand, within CA265_RS13280 at 3299.145 kb on - strand, within CA265_RS13280 at 3299.145 kb on - strand, within CA265_RS13280 at 3299.149 kb on - strand, within CA265_RS13280 at 3299.158 kb on - strand, within CA265_RS13280 at 3299.281 kb on - strand at 3299.446 kb on - strand, within CA265_RS13285 at 3299.455 kb on + strand, within CA265_RS13285 at 3299.499 kb on + strand, within CA265_RS13285 at 3299.535 kb on + strand, within CA265_RS13285 at 3299.535 kb on + strand, within CA265_RS13285 at 3299.591 kb on - strand, within CA265_RS13285 at 3299.613 kb on + strand, within CA265_RS13285 at 3299.614 kb on - strand, within CA265_RS13285 at 3299.654 kb on - strand, within CA265_RS13285 at 3299.706 kb on + strand, within CA265_RS13285 at 3299.750 kb on - strand, within CA265_RS13285 at 3299.754 kb on + strand, within CA265_RS13285 at 3299.876 kb on + strand, within CA265_RS13285 at 3299.926 kb on - strand, within CA265_RS13285 at 3299.974 kb on + strand, within CA265_RS13285 at 3300.067 kb on + strand, within CA265_RS13285 at 3300.144 kb on + strand, within CA265_RS13285 at 3300.145 kb on - strand, within CA265_RS13285 at 3300.157 kb on + strand, within CA265_RS13285 at 3300.169 kb on - strand, within CA265_RS13285 at 3300.179 kb on + strand, within CA265_RS13285 at 3300.180 kb on - strand, within CA265_RS13285 at 3300.180 kb on - strand, within CA265_RS13285 at 3300.180 kb on - strand, within CA265_RS13285 at 3300.243 kb on + strand, within CA265_RS13285 at 3300.268 kb on - strand, within CA265_RS13285 at 3300.294 kb on + strand, within CA265_RS13285
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.016 mg/ml remove 3,297,704 + CA265_RS13275 0.44 -1.1 3,297,704 + CA265_RS13275 0.44 +0.0 3,297,745 + CA265_RS13275 0.48 -1.8 3,297,812 - CA265_RS13275 0.54 -0.6 3,297,830 + CA265_RS13275 0.56 -1.5 3,297,831 - CA265_RS13275 0.56 -0.8 3,297,886 - CA265_RS13275 0.61 -0.2 3,297,967 + CA265_RS13275 0.68 -2.1 3,297,972 - CA265_RS13275 0.68 -1.2 3,298,049 - CA265_RS13275 0.75 -1.5 3,298,071 - CA265_RS13275 0.77 -0.8 3,298,215 + -1.2 3,298,216 - +0.3 3,298,216 - +1.5 3,298,217 - -0.4 3,298,252 - -2.7 3,298,257 + +0.0 3,298,258 - -0.8 3,298,258 - -1.6 3,298,275 + +0.2 3,298,430 + +0.6 3,298,573 + +0.4 3,298,574 - -0.0 3,298,583 + -3.4 3,298,583 + -0.7 3,298,587 + -1.3 3,298,587 + -0.1 3,298,588 - +0.1 3,298,635 + -1.9 3,298,635 + +1.2 3,298,668 + +0.9 3,298,669 - -1.7 3,298,675 + -0.5 3,298,682 + +0.0 3,298,683 - -0.6 3,298,698 - +0.5 3,298,711 + +0.7 3,298,757 - CA265_RS13280 0.12 -0.1 3,298,799 + CA265_RS13280 0.19 -0.5 3,298,799 + CA265_RS13280 0.19 -0.5 3,298,800 - CA265_RS13280 0.19 -0.9 3,298,800 - CA265_RS13280 0.19 -1.2 3,298,800 - CA265_RS13280 0.19 -0.7 3,298,986 + CA265_RS13280 0.50 +0.3 3,298,988 + CA265_RS13280 0.50 -2.6 3,298,989 - CA265_RS13280 0.50 +0.5 3,298,989 - CA265_RS13280 0.50 -0.2 3,299,103 + CA265_RS13280 0.69 +0.6 3,299,129 - CA265_RS13280 0.73 -0.8 3,299,144 + CA265_RS13280 0.75 -0.7 3,299,144 + CA265_RS13280 0.75 +0.5 3,299,145 - CA265_RS13280 0.75 -1.1 3,299,145 - CA265_RS13280 0.75 -0.5 3,299,145 - CA265_RS13280 0.75 -2.9 3,299,149 - CA265_RS13280 0.76 -0.4 3,299,158 - CA265_RS13280 0.78 -0.7 3,299,281 - -0.9 3,299,446 - CA265_RS13285 0.10 -0.4 3,299,455 + CA265_RS13285 0.11 -1.2 3,299,499 + CA265_RS13285 0.14 -0.6 3,299,535 + CA265_RS13285 0.17 +0.6 3,299,535 + CA265_RS13285 0.17 -0.6 3,299,591 - CA265_RS13285 0.21 -1.5 3,299,613 + CA265_RS13285 0.22 +1.0 3,299,614 - CA265_RS13285 0.22 -0.4 3,299,654 - CA265_RS13285 0.25 -3.0 3,299,706 + CA265_RS13285 0.29 -0.8 3,299,750 - CA265_RS13285 0.32 -1.2 3,299,754 + CA265_RS13285 0.33 +0.3 3,299,876 + CA265_RS13285 0.41 -0.4 3,299,926 - CA265_RS13285 0.45 -0.4 3,299,974 + CA265_RS13285 0.49 -0.5 3,300,067 + CA265_RS13285 0.55 +1.3 3,300,144 + CA265_RS13285 0.61 +0.2 3,300,145 - CA265_RS13285 0.61 -1.8 3,300,157 + CA265_RS13285 0.62 -0.0 3,300,169 - CA265_RS13285 0.63 -3.0 3,300,179 + CA265_RS13285 0.63 -0.7 3,300,180 - CA265_RS13285 0.64 -0.3 3,300,180 - CA265_RS13285 0.64 -0.8 3,300,180 - CA265_RS13285 0.64 -0.2 3,300,243 + CA265_RS13285 0.68 -0.2 3,300,268 - CA265_RS13285 0.70 -0.1 3,300,294 + CA265_RS13285 0.72 +0.4
Or see this region's nucleotide sequence