Experiment: R2A with Polymyxin B sulfate 0.016 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS11035 and CA265_RS11040 are separated by 224 nucleotides CA265_RS11040 and CA265_RS11045 are separated by 7 nucleotides CA265_RS11045 and CA265_RS11050 are separated by 281 nucleotides CA265_RS11050 and CA265_RS11055 are separated by 269 nucleotides
CA265_RS11035: CA265_RS11035 - phosphohydrolase, at 2,709,348 to 2,710,202
_RS11035
CA265_RS11040: CA265_RS11040 - hypothetical protein, at 2,710,427 to 2,711,080
_RS11040
CA265_RS11045: CA265_RS11045 - ABC transporter, at 2,711,088 to 2,712,386
_RS11045
CA265_RS11050: CA265_RS11050 - hypothetical protein, at 2,712,668 to 2,713,024
_RS11050
CA265_RS11055: CA265_RS11055 - hypothetical protein, at 2,713,294 to 2,713,896
_RS11055
Position (kb)
2711
2712
2713 Strain fitness (log2 ratio)
-2
-1
0
1 at 2710.134 kb on - strand at 2710.211 kb on - strand at 2710.228 kb on - strand at 2710.284 kb on - strand at 2710.304 kb on + strand at 2710.348 kb on + strand at 2710.348 kb on + strand at 2710.407 kb on + strand at 2710.413 kb on + strand at 2710.424 kb on + strand at 2710.426 kb on + strand at 2710.427 kb on - strand at 2710.461 kb on + strand at 2710.462 kb on - strand at 2710.462 kb on - strand at 2710.504 kb on + strand, within CA265_RS11040 at 2710.573 kb on - strand, within CA265_RS11040 at 2710.573 kb on - strand, within CA265_RS11040 at 2710.647 kb on - strand, within CA265_RS11040 at 2710.649 kb on - strand, within CA265_RS11040 at 2710.679 kb on + strand, within CA265_RS11040 at 2710.680 kb on - strand, within CA265_RS11040 at 2710.680 kb on - strand, within CA265_RS11040 at 2710.685 kb on + strand, within CA265_RS11040 at 2710.724 kb on + strand, within CA265_RS11040 at 2710.725 kb on - strand, within CA265_RS11040 at 2710.756 kb on + strand, within CA265_RS11040 at 2710.813 kb on + strand, within CA265_RS11040 at 2710.827 kb on + strand, within CA265_RS11040 at 2710.832 kb on - strand, within CA265_RS11040 at 2710.865 kb on - strand, within CA265_RS11040 at 2711.014 kb on + strand, within CA265_RS11040 at 2711.079 kb on - strand at 2711.114 kb on + strand at 2711.120 kb on + strand at 2711.172 kb on + strand at 2711.175 kb on - strand at 2711.175 kb on - strand at 2711.178 kb on - strand at 2711.228 kb on - strand, within CA265_RS11045 at 2711.255 kb on + strand, within CA265_RS11045 at 2711.321 kb on + strand, within CA265_RS11045 at 2711.337 kb on + strand, within CA265_RS11045 at 2711.341 kb on + strand, within CA265_RS11045 at 2711.582 kb on - strand, within CA265_RS11045 at 2711.631 kb on - strand, within CA265_RS11045 at 2711.634 kb on + strand, within CA265_RS11045 at 2711.635 kb on - strand, within CA265_RS11045 at 2711.676 kb on + strand, within CA265_RS11045 at 2711.751 kb on + strand, within CA265_RS11045 at 2711.752 kb on - strand, within CA265_RS11045 at 2711.752 kb on - strand, within CA265_RS11045 at 2711.807 kb on - strand, within CA265_RS11045 at 2711.914 kb on + strand, within CA265_RS11045 at 2711.991 kb on - strand, within CA265_RS11045 at 2712.047 kb on - strand, within CA265_RS11045 at 2712.071 kb on - strand, within CA265_RS11045 at 2712.132 kb on + strand, within CA265_RS11045 at 2712.177 kb on + strand, within CA265_RS11045 at 2712.177 kb on + strand, within CA265_RS11045 at 2712.177 kb on + strand, within CA265_RS11045 at 2712.177 kb on + strand, within CA265_RS11045 at 2712.178 kb on - strand, within CA265_RS11045 at 2712.179 kb on + strand, within CA265_RS11045 at 2712.186 kb on - strand, within CA265_RS11045 at 2712.229 kb on - strand, within CA265_RS11045 at 2712.229 kb on - strand, within CA265_RS11045 at 2712.230 kb on + strand, within CA265_RS11045 at 2712.231 kb on - strand, within CA265_RS11045 at 2712.234 kb on + strand, within CA265_RS11045 at 2712.234 kb on + strand, within CA265_RS11045 at 2712.235 kb on - strand, within CA265_RS11045 at 2712.327 kb on - strand at 2712.335 kb on - strand at 2712.335 kb on - strand at 2712.382 kb on + strand at 2712.391 kb on + strand at 2712.392 kb on - strand at 2712.434 kb on + strand at 2712.467 kb on + strand at 2712.468 kb on - strand at 2712.513 kb on + strand at 2712.516 kb on - strand at 2712.525 kb on + strand at 2712.537 kb on - strand at 2712.570 kb on - strand at 2712.595 kb on + strand at 2712.598 kb on - strand at 2712.726 kb on + strand, within CA265_RS11050 at 2712.761 kb on + strand, within CA265_RS11050 at 2712.794 kb on + strand, within CA265_RS11050 at 2712.795 kb on - strand, within CA265_RS11050 at 2712.795 kb on - strand, within CA265_RS11050 at 2712.820 kb on + strand, within CA265_RS11050 at 2712.873 kb on - strand, within CA265_RS11050 at 2712.908 kb on - strand, within CA265_RS11050 at 2712.908 kb on - strand, within CA265_RS11050 at 2712.908 kb on - strand, within CA265_RS11050 at 2712.974 kb on + strand, within CA265_RS11050 at 2713.008 kb on - strand at 2713.108 kb on + strand at 2713.157 kb on - strand at 2713.157 kb on - strand at 2713.216 kb on + strand at 2713.229 kb on - strand at 2713.274 kb on - strand at 2713.362 kb on + strand, within CA265_RS11055 at 2713.362 kb on + strand, within CA265_RS11055 at 2713.366 kb on - strand, within CA265_RS11055
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.016 mg/ml remove 2,710,134 - -0.1 2,710,211 - -0.0 2,710,228 - +0.0 2,710,284 - -0.3 2,710,304 + +0.8 2,710,348 + +0.0 2,710,348 + +0.2 2,710,407 + -0.1 2,710,413 + -0.7 2,710,424 + -0.7 2,710,426 + +0.3 2,710,427 - -0.4 2,710,461 + -0.1 2,710,462 - +0.5 2,710,462 - -1.0 2,710,504 + CA265_RS11040 0.12 +0.7 2,710,573 - CA265_RS11040 0.22 -0.6 2,710,573 - CA265_RS11040 0.22 -1.8 2,710,647 - CA265_RS11040 0.34 -0.0 2,710,649 - CA265_RS11040 0.34 -0.1 2,710,679 + CA265_RS11040 0.39 -0.2 2,710,680 - CA265_RS11040 0.39 -0.9 2,710,680 - CA265_RS11040 0.39 +0.1 2,710,685 + CA265_RS11040 0.39 -1.5 2,710,724 + CA265_RS11040 0.45 -1.2 2,710,725 - CA265_RS11040 0.46 +0.4 2,710,756 + CA265_RS11040 0.50 +0.4 2,710,813 + CA265_RS11040 0.59 -1.0 2,710,827 + CA265_RS11040 0.61 -0.2 2,710,832 - CA265_RS11040 0.62 +0.2 2,710,865 - CA265_RS11040 0.67 +0.6 2,711,014 + CA265_RS11040 0.90 -0.9 2,711,079 - +0.2 2,711,114 + -0.2 2,711,120 + -0.4 2,711,172 + +0.0 2,711,175 - -0.6 2,711,175 - +0.2 2,711,178 - +0.3 2,711,228 - CA265_RS11045 0.11 -1.2 2,711,255 + CA265_RS11045 0.13 -0.5 2,711,321 + CA265_RS11045 0.18 -0.6 2,711,337 + CA265_RS11045 0.19 +0.3 2,711,341 + CA265_RS11045 0.19 -0.0 2,711,582 - CA265_RS11045 0.38 +0.1 2,711,631 - CA265_RS11045 0.42 -1.1 2,711,634 + CA265_RS11045 0.42 -1.9 2,711,635 - CA265_RS11045 0.42 +0.0 2,711,676 + CA265_RS11045 0.45 -0.9 2,711,751 + CA265_RS11045 0.51 +0.5 2,711,752 - CA265_RS11045 0.51 +0.2 2,711,752 - CA265_RS11045 0.51 -0.6 2,711,807 - CA265_RS11045 0.55 -0.7 2,711,914 + CA265_RS11045 0.64 -0.3 2,711,991 - CA265_RS11045 0.70 -0.5 2,712,047 - CA265_RS11045 0.74 +1.1 2,712,071 - CA265_RS11045 0.76 +0.8 2,712,132 + CA265_RS11045 0.80 -0.4 2,712,177 + CA265_RS11045 0.84 +0.5 2,712,177 + CA265_RS11045 0.84 -0.1 2,712,177 + CA265_RS11045 0.84 -0.4 2,712,177 + CA265_RS11045 0.84 +0.4 2,712,178 - CA265_RS11045 0.84 +1.1 2,712,179 + CA265_RS11045 0.84 +0.4 2,712,186 - CA265_RS11045 0.85 -0.6 2,712,229 - CA265_RS11045 0.88 -0.2 2,712,229 - CA265_RS11045 0.88 -0.1 2,712,230 + CA265_RS11045 0.88 +0.5 2,712,231 - CA265_RS11045 0.88 -0.2 2,712,234 + CA265_RS11045 0.88 -0.8 2,712,234 + CA265_RS11045 0.88 +0.2 2,712,235 - CA265_RS11045 0.88 -0.2 2,712,327 - -0.3 2,712,335 - -0.2 2,712,335 - +0.9 2,712,382 + -0.2 2,712,391 + -0.6 2,712,392 - -0.2 2,712,434 + +0.3 2,712,467 + -0.3 2,712,468 - -0.6 2,712,513 + -1.6 2,712,516 - +0.7 2,712,525 + +0.4 2,712,537 - +0.8 2,712,570 - +0.2 2,712,595 + +0.2 2,712,598 - +0.8 2,712,726 + CA265_RS11050 0.16 -0.0 2,712,761 + CA265_RS11050 0.26 -0.0 2,712,794 + CA265_RS11050 0.35 +0.3 2,712,795 - CA265_RS11050 0.36 -1.5 2,712,795 - CA265_RS11050 0.36 -0.2 2,712,820 + CA265_RS11050 0.43 +0.4 2,712,873 - CA265_RS11050 0.57 +1.7 2,712,908 - CA265_RS11050 0.67 +0.6 2,712,908 - CA265_RS11050 0.67 -0.5 2,712,908 - CA265_RS11050 0.67 +0.6 2,712,974 + CA265_RS11050 0.86 -2.4 2,713,008 - +0.3 2,713,108 + -0.1 2,713,157 - +0.1 2,713,157 - +0.4 2,713,216 + +0.7 2,713,229 - -0.4 2,713,274 - -0.0 2,713,362 + CA265_RS11055 0.11 +1.0 2,713,362 + CA265_RS11055 0.11 -0.5 2,713,366 - CA265_RS11055 0.12 +1.2
Or see this region's nucleotide sequence