Experiment: R2A with Polymyxin B sulfate 0.016 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS11020 and CA265_RS11025 are separated by 251 nucleotides CA265_RS11025 and CA265_RS11030 are separated by 57 nucleotides CA265_RS11030 and CA265_RS11035 are separated by 160 nucleotides CA265_RS11035 and CA265_RS11040 are separated by 224 nucleotides CA265_RS11040 and CA265_RS11045 are separated by 7 nucleotides
CA265_RS11020: CA265_RS11020 - hypothetical protein, at 2,704,968 to 2,708,525
_RS11020
CA265_RS11025: CA265_RS11025 - hypothetical protein, at 2,708,777 to 2,708,989
_RS11025
CA265_RS11030: CA265_RS11030 - twin-arginine translocase TatA/TatE family subunit, at 2,709,047 to 2,709,187
_RS11030
CA265_RS11035: CA265_RS11035 - phosphohydrolase, at 2,709,348 to 2,710,202
_RS11035
CA265_RS11040: CA265_RS11040 - hypothetical protein, at 2,710,427 to 2,711,080
_RS11040
CA265_RS11045: CA265_RS11045 - ABC transporter, at 2,711,088 to 2,712,386
_RS11045
Position (kb)
2709
2710
2711 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2708.400 kb on - strand at 2708.400 kb on - strand at 2708.400 kb on - strand at 2708.400 kb on - strand at 2708.423 kb on - strand at 2708.581 kb on + strand at 2708.582 kb on - strand at 2708.675 kb on + strand at 2708.704 kb on - strand at 2708.771 kb on - strand at 2708.860 kb on + strand, within CA265_RS11025 at 2708.861 kb on - strand, within CA265_RS11025 at 2708.910 kb on - strand, within CA265_RS11025 at 2709.048 kb on + strand at 2709.049 kb on - strand at 2709.053 kb on - strand at 2709.162 kb on - strand, within CA265_RS11030 at 2709.187 kb on + strand at 2709.187 kb on + strand at 2709.195 kb on + strand at 2709.196 kb on - strand at 2709.207 kb on + strand at 2709.207 kb on + strand at 2709.209 kb on + strand at 2709.294 kb on + strand at 2709.295 kb on - strand at 2709.318 kb on + strand at 2709.408 kb on - strand at 2709.464 kb on - strand, within CA265_RS11035 at 2709.522 kb on + strand, within CA265_RS11035 at 2709.580 kb on - strand, within CA265_RS11035 at 2709.584 kb on + strand, within CA265_RS11035 at 2709.597 kb on - strand, within CA265_RS11035 at 2709.602 kb on + strand, within CA265_RS11035 at 2709.602 kb on + strand, within CA265_RS11035 at 2709.602 kb on + strand, within CA265_RS11035 at 2709.603 kb on - strand, within CA265_RS11035 at 2709.603 kb on - strand, within CA265_RS11035 at 2709.839 kb on - strand, within CA265_RS11035 at 2709.856 kb on - strand, within CA265_RS11035 at 2709.856 kb on - strand, within CA265_RS11035 at 2709.908 kb on - strand, within CA265_RS11035 at 2709.921 kb on - strand, within CA265_RS11035 at 2709.968 kb on + strand, within CA265_RS11035 at 2710.011 kb on + strand, within CA265_RS11035 at 2710.011 kb on + strand, within CA265_RS11035 at 2710.134 kb on - strand at 2710.211 kb on - strand at 2710.228 kb on - strand at 2710.284 kb on - strand at 2710.304 kb on + strand at 2710.348 kb on + strand at 2710.348 kb on + strand at 2710.407 kb on + strand at 2710.413 kb on + strand at 2710.424 kb on + strand at 2710.426 kb on + strand at 2710.427 kb on - strand at 2710.461 kb on + strand at 2710.462 kb on - strand at 2710.462 kb on - strand at 2710.504 kb on + strand, within CA265_RS11040 at 2710.573 kb on - strand, within CA265_RS11040 at 2710.573 kb on - strand, within CA265_RS11040 at 2710.647 kb on - strand, within CA265_RS11040 at 2710.649 kb on - strand, within CA265_RS11040 at 2710.679 kb on + strand, within CA265_RS11040 at 2710.680 kb on - strand, within CA265_RS11040 at 2710.680 kb on - strand, within CA265_RS11040 at 2710.685 kb on + strand, within CA265_RS11040 at 2710.724 kb on + strand, within CA265_RS11040 at 2710.725 kb on - strand, within CA265_RS11040 at 2710.756 kb on + strand, within CA265_RS11040 at 2710.813 kb on + strand, within CA265_RS11040 at 2710.827 kb on + strand, within CA265_RS11040 at 2710.832 kb on - strand, within CA265_RS11040 at 2710.865 kb on - strand, within CA265_RS11040 at 2711.014 kb on + strand, within CA265_RS11040 at 2711.079 kb on - strand at 2711.114 kb on + strand at 2711.120 kb on + strand at 2711.172 kb on + strand at 2711.175 kb on - strand at 2711.175 kb on - strand at 2711.178 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.016 mg/ml remove 2,708,400 - -0.8 2,708,400 - +0.7 2,708,400 - +0.4 2,708,400 - -0.3 2,708,423 - -0.6 2,708,581 + +0.1 2,708,582 - +0.5 2,708,675 + +0.1 2,708,704 - +0.5 2,708,771 - -0.3 2,708,860 + CA265_RS11025 0.39 -0.2 2,708,861 - CA265_RS11025 0.39 -0.7 2,708,910 - CA265_RS11025 0.62 -1.2 2,709,048 + +0.7 2,709,049 - -0.9 2,709,053 - -0.0 2,709,162 - CA265_RS11030 0.82 -0.7 2,709,187 + +0.2 2,709,187 + +0.3 2,709,195 + -0.1 2,709,196 - -0.4 2,709,207 + +1.3 2,709,207 + -0.1 2,709,209 + +0.3 2,709,294 + -0.7 2,709,295 - +0.2 2,709,318 + +0.2 2,709,408 - -0.8 2,709,464 - CA265_RS11035 0.14 +0.0 2,709,522 + CA265_RS11035 0.20 +1.8 2,709,580 - CA265_RS11035 0.27 -1.6 2,709,584 + CA265_RS11035 0.28 +0.0 2,709,597 - CA265_RS11035 0.29 -1.7 2,709,602 + CA265_RS11035 0.30 +1.1 2,709,602 + CA265_RS11035 0.30 -0.6 2,709,602 + CA265_RS11035 0.30 +0.2 2,709,603 - CA265_RS11035 0.30 -0.6 2,709,603 - CA265_RS11035 0.30 -0.9 2,709,839 - CA265_RS11035 0.57 -0.8 2,709,856 - CA265_RS11035 0.59 -0.7 2,709,856 - CA265_RS11035 0.59 +2.1 2,709,908 - CA265_RS11035 0.65 +0.2 2,709,921 - CA265_RS11035 0.67 -0.8 2,709,968 + CA265_RS11035 0.73 +0.2 2,710,011 + CA265_RS11035 0.78 +0.4 2,710,011 + CA265_RS11035 0.78 -0.0 2,710,134 - -0.1 2,710,211 - -0.0 2,710,228 - +0.0 2,710,284 - -0.3 2,710,304 + +0.8 2,710,348 + +0.0 2,710,348 + +0.2 2,710,407 + -0.1 2,710,413 + -0.7 2,710,424 + -0.7 2,710,426 + +0.3 2,710,427 - -0.4 2,710,461 + -0.1 2,710,462 - +0.5 2,710,462 - -1.0 2,710,504 + CA265_RS11040 0.12 +0.7 2,710,573 - CA265_RS11040 0.22 -0.6 2,710,573 - CA265_RS11040 0.22 -1.8 2,710,647 - CA265_RS11040 0.34 -0.0 2,710,649 - CA265_RS11040 0.34 -0.1 2,710,679 + CA265_RS11040 0.39 -0.2 2,710,680 - CA265_RS11040 0.39 -0.9 2,710,680 - CA265_RS11040 0.39 +0.1 2,710,685 + CA265_RS11040 0.39 -1.5 2,710,724 + CA265_RS11040 0.45 -1.2 2,710,725 - CA265_RS11040 0.46 +0.4 2,710,756 + CA265_RS11040 0.50 +0.4 2,710,813 + CA265_RS11040 0.59 -1.0 2,710,827 + CA265_RS11040 0.61 -0.2 2,710,832 - CA265_RS11040 0.62 +0.2 2,710,865 - CA265_RS11040 0.67 +0.6 2,711,014 + CA265_RS11040 0.90 -0.9 2,711,079 - +0.2 2,711,114 + -0.2 2,711,120 + -0.4 2,711,172 + +0.0 2,711,175 - -0.6 2,711,175 - +0.2 2,711,178 - +0.3
Or see this region's nucleotide sequence