Experiment: R2A with Polymyxin B sulfate 0.016 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS04455 and CA265_RS04460 are separated by 52 nucleotides CA265_RS04460 and CA265_RS04465 are separated by 131 nucleotides CA265_RS04465 and CA265_RS04470 are separated by 82 nucleotides
CA265_RS04455: CA265_RS04455 - hypothetical protein, at 1,066,568 to 1,067,851
_RS04455
CA265_RS04460: CA265_RS04460 - hypothetical protein, at 1,067,904 to 1,068,119
_RS04460
CA265_RS04465: CA265_RS04465 - PspC family transcriptional regulator, at 1,068,251 to 1,068,469
_RS04465
CA265_RS04470: CA265_RS04470 - hypothetical protein, at 1,068,552 to 1,069,208
_RS04470
Position (kb)
1068
1069 Strain fitness (log2 ratio)
-2
-1
0
1 at 1067.324 kb on + strand, within CA265_RS04455 at 1067.332 kb on + strand, within CA265_RS04455 at 1067.419 kb on - strand, within CA265_RS04455 at 1067.419 kb on - strand, within CA265_RS04455 at 1067.425 kb on - strand, within CA265_RS04455 at 1067.460 kb on + strand, within CA265_RS04455 at 1067.488 kb on - strand, within CA265_RS04455 at 1067.584 kb on - strand, within CA265_RS04455 at 1067.584 kb on - strand, within CA265_RS04455 at 1067.588 kb on - strand, within CA265_RS04455 at 1067.591 kb on - strand, within CA265_RS04455 at 1067.607 kb on + strand, within CA265_RS04455 at 1067.651 kb on + strand, within CA265_RS04455 at 1067.651 kb on + strand, within CA265_RS04455 at 1067.652 kb on - strand, within CA265_RS04455 at 1067.655 kb on - strand, within CA265_RS04455 at 1067.655 kb on - strand, within CA265_RS04455 at 1067.657 kb on - strand, within CA265_RS04455 at 1067.812 kb on - strand at 1067.812 kb on - strand at 1067.812 kb on - strand at 1067.888 kb on - strand at 1067.898 kb on + strand at 1067.898 kb on + strand at 1067.924 kb on - strand at 1067.977 kb on + strand, within CA265_RS04460 at 1067.984 kb on + strand, within CA265_RS04460 at 1067.985 kb on - strand, within CA265_RS04460 at 1068.011 kb on + strand, within CA265_RS04460 at 1068.087 kb on - strand, within CA265_RS04460 at 1068.135 kb on + strand at 1068.154 kb on - strand at 1068.228 kb on + strand at 1068.242 kb on - strand at 1068.273 kb on - strand, within CA265_RS04465 at 1068.302 kb on - strand, within CA265_RS04465 at 1068.305 kb on + strand, within CA265_RS04465 at 1068.317 kb on - strand, within CA265_RS04465 at 1068.359 kb on + strand, within CA265_RS04465 at 1068.394 kb on - strand, within CA265_RS04465 at 1068.435 kb on - strand, within CA265_RS04465 at 1068.435 kb on - strand at 1068.459 kb on + strand at 1068.686 kb on + strand, within CA265_RS04470 at 1068.686 kb on + strand, within CA265_RS04470 at 1068.686 kb on + strand, within CA265_RS04470 at 1068.720 kb on + strand, within CA265_RS04470 at 1068.762 kb on + strand, within CA265_RS04470 at 1068.762 kb on + strand, within CA265_RS04470 at 1068.779 kb on + strand, within CA265_RS04470 at 1068.789 kb on - strand, within CA265_RS04470 at 1068.879 kb on - strand, within CA265_RS04470 at 1068.902 kb on - strand, within CA265_RS04470 at 1068.950 kb on + strand, within CA265_RS04470 at 1069.047 kb on + strand, within CA265_RS04470 at 1069.048 kb on - strand, within CA265_RS04470 at 1069.076 kb on - strand, within CA265_RS04470 at 1069.087 kb on - strand, within CA265_RS04470 at 1069.089 kb on + strand, within CA265_RS04470 at 1069.090 kb on - strand, within CA265_RS04470 at 1069.118 kb on + strand, within CA265_RS04470 at 1069.156 kb on - strand at 1069.211 kb on + strand at 1069.211 kb on + strand at 1069.212 kb on - strand at 1069.275 kb on - strand at 1069.276 kb on + strand at 1069.276 kb on + strand at 1069.276 kb on + strand at 1069.276 kb on + strand at 1069.332 kb on - strand at 1069.415 kb on + strand at 1069.415 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.016 mg/ml remove 1,067,324 + CA265_RS04455 0.59 +0.7 1,067,332 + CA265_RS04455 0.60 +0.4 1,067,419 - CA265_RS04455 0.66 +0.7 1,067,419 - CA265_RS04455 0.66 -0.1 1,067,425 - CA265_RS04455 0.67 +0.0 1,067,460 + CA265_RS04455 0.69 -0.5 1,067,488 - CA265_RS04455 0.72 +0.1 1,067,584 - CA265_RS04455 0.79 +0.5 1,067,584 - CA265_RS04455 0.79 +0.0 1,067,588 - CA265_RS04455 0.79 -0.4 1,067,591 - CA265_RS04455 0.80 +0.8 1,067,607 + CA265_RS04455 0.81 +0.0 1,067,651 + CA265_RS04455 0.84 -0.1 1,067,651 + CA265_RS04455 0.84 -0.2 1,067,652 - CA265_RS04455 0.84 -0.8 1,067,655 - CA265_RS04455 0.85 +0.1 1,067,655 - CA265_RS04455 0.85 +0.1 1,067,657 - CA265_RS04455 0.85 +0.1 1,067,812 - +0.6 1,067,812 - -1.4 1,067,812 - +0.1 1,067,888 - -0.6 1,067,898 + -0.9 1,067,898 + -0.5 1,067,924 - -1.0 1,067,977 + CA265_RS04460 0.34 -1.2 1,067,984 + CA265_RS04460 0.37 -0.1 1,067,985 - CA265_RS04460 0.38 -0.9 1,068,011 + CA265_RS04460 0.50 -0.6 1,068,087 - CA265_RS04460 0.85 -0.2 1,068,135 + +0.0 1,068,154 - -0.6 1,068,228 + +0.2 1,068,242 - +0.7 1,068,273 - CA265_RS04465 0.10 +1.0 1,068,302 - CA265_RS04465 0.23 +0.6 1,068,305 + CA265_RS04465 0.25 +0.5 1,068,317 - CA265_RS04465 0.30 +0.8 1,068,359 + CA265_RS04465 0.49 -0.5 1,068,394 - CA265_RS04465 0.65 -0.4 1,068,435 - CA265_RS04465 0.84 +0.9 1,068,435 - -1.3 1,068,459 + +0.7 1,068,686 + CA265_RS04470 0.20 +0.6 1,068,686 + CA265_RS04470 0.20 +0.0 1,068,686 + CA265_RS04470 0.20 -0.4 1,068,720 + CA265_RS04470 0.26 -0.2 1,068,762 + CA265_RS04470 0.32 +0.2 1,068,762 + CA265_RS04470 0.32 -0.1 1,068,779 + CA265_RS04470 0.35 -2.0 1,068,789 - CA265_RS04470 0.36 +0.5 1,068,879 - CA265_RS04470 0.50 +0.1 1,068,902 - CA265_RS04470 0.53 -0.7 1,068,950 + CA265_RS04470 0.61 +0.1 1,069,047 + CA265_RS04470 0.75 -0.1 1,069,048 - CA265_RS04470 0.75 +0.1 1,069,076 - CA265_RS04470 0.80 +0.7 1,069,087 - CA265_RS04470 0.81 +0.3 1,069,089 + CA265_RS04470 0.82 -0.2 1,069,090 - CA265_RS04470 0.82 +0.4 1,069,118 + CA265_RS04470 0.86 -0.4 1,069,156 - -0.2 1,069,211 + +0.1 1,069,211 + -0.7 1,069,212 - +0.6 1,069,275 - +0.0 1,069,276 + -0.6 1,069,276 + -1.1 1,069,276 + -0.3 1,069,276 + -0.2 1,069,332 - +1.5 1,069,415 + +0.0 1,069,415 + +0.0
Or see this region's nucleotide sequence