Experiment: R2A with Polymyxin B sulfate 0.016 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS04030 and CA265_RS04035 are separated by 30 nucleotides CA265_RS04035 and CA265_RS04040 are separated by 36 nucleotides CA265_RS04040 and CA265_RS04045 are separated by 134 nucleotides CA265_RS04045 and CA265_RS04050 are separated by 66 nucleotides
CA265_RS04030: CA265_RS04030 - alpha-galactosidase, at 966,761 to 967,960
_RS04030
CA265_RS04035: CA265_RS04035 - hypothetical protein, at 967,991 to 969,499
_RS04035
CA265_RS04040: CA265_RS04040 - hypothetical protein, at 969,536 to 969,853
_RS04040
CA265_RS04045: CA265_RS04045 - hypothetical protein, at 969,988 to 970,380
_RS04045
CA265_RS04050: CA265_RS04050 - nucleoside-diphosphate sugar epimerase, at 970,447 to 971,322
_RS04050
Position (kb)
967
968
969
970 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 967.039 kb on - strand, within CA265_RS04030 at 967.088 kb on + strand, within CA265_RS04030 at 967.089 kb on - strand, within CA265_RS04030 at 967.123 kb on + strand, within CA265_RS04030 at 967.184 kb on + strand, within CA265_RS04030 at 967.282 kb on + strand, within CA265_RS04030 at 967.282 kb on + strand, within CA265_RS04030 at 967.282 kb on + strand, within CA265_RS04030 at 967.282 kb on + strand, within CA265_RS04030 at 967.294 kb on + strand, within CA265_RS04030 at 967.295 kb on - strand, within CA265_RS04030 at 967.352 kb on - strand, within CA265_RS04030 at 967.367 kb on + strand, within CA265_RS04030 at 967.368 kb on - strand, within CA265_RS04030 at 967.381 kb on + strand, within CA265_RS04030 at 967.381 kb on - strand, within CA265_RS04030 at 967.417 kb on + strand, within CA265_RS04030 at 967.417 kb on + strand, within CA265_RS04030 at 967.418 kb on - strand, within CA265_RS04030 at 967.418 kb on - strand, within CA265_RS04030 at 967.418 kb on - strand, within CA265_RS04030 at 967.421 kb on + strand, within CA265_RS04030 at 967.506 kb on + strand, within CA265_RS04030 at 967.506 kb on + strand, within CA265_RS04030 at 967.506 kb on + strand, within CA265_RS04030 at 967.538 kb on - strand, within CA265_RS04030 at 967.587 kb on - strand, within CA265_RS04030 at 967.597 kb on + strand, within CA265_RS04030 at 967.598 kb on - strand, within CA265_RS04030 at 967.609 kb on - strand, within CA265_RS04030 at 967.675 kb on - strand, within CA265_RS04030 at 967.853 kb on + strand at 967.853 kb on + strand at 967.885 kb on - strand at 967.917 kb on + strand at 967.959 kb on - strand at 967.970 kb on + strand at 968.002 kb on - strand at 968.080 kb on + strand at 968.170 kb on + strand, within CA265_RS04035 at 968.171 kb on - strand, within CA265_RS04035 at 968.291 kb on - strand, within CA265_RS04035 at 968.336 kb on + strand, within CA265_RS04035 at 968.336 kb on + strand, within CA265_RS04035 at 968.337 kb on - strand, within CA265_RS04035 at 968.337 kb on - strand, within CA265_RS04035 at 968.370 kb on - strand, within CA265_RS04035 at 968.403 kb on + strand, within CA265_RS04035 at 968.403 kb on + strand, within CA265_RS04035 at 968.404 kb on - strand, within CA265_RS04035 at 968.412 kb on + strand, within CA265_RS04035 at 968.434 kb on + strand, within CA265_RS04035 at 968.434 kb on + strand, within CA265_RS04035 at 968.435 kb on - strand, within CA265_RS04035 at 968.451 kb on - strand, within CA265_RS04035 at 968.457 kb on + strand, within CA265_RS04035 at 968.515 kb on - strand, within CA265_RS04035 at 968.559 kb on - strand, within CA265_RS04035 at 968.561 kb on - strand, within CA265_RS04035 at 968.561 kb on - strand, within CA265_RS04035 at 968.608 kb on + strand, within CA265_RS04035 at 968.677 kb on - strand, within CA265_RS04035 at 968.784 kb on + strand, within CA265_RS04035 at 968.872 kb on + strand, within CA265_RS04035 at 968.872 kb on + strand, within CA265_RS04035 at 968.885 kb on + strand, within CA265_RS04035 at 968.886 kb on - strand, within CA265_RS04035 at 968.886 kb on - strand, within CA265_RS04035 at 968.970 kb on + strand, within CA265_RS04035 at 968.987 kb on - strand, within CA265_RS04035 at 969.097 kb on + strand, within CA265_RS04035 at 969.098 kb on - strand, within CA265_RS04035 at 969.299 kb on + strand, within CA265_RS04035 at 969.369 kb on - strand at 969.374 kb on - strand at 969.479 kb on - strand at 969.543 kb on + strand at 969.584 kb on - strand, within CA265_RS04040 at 969.595 kb on + strand, within CA265_RS04040 at 969.596 kb on - strand, within CA265_RS04040 at 969.596 kb on - strand, within CA265_RS04040 at 969.600 kb on - strand, within CA265_RS04040 at 969.892 kb on - strand at 969.927 kb on + strand at 969.928 kb on - strand at 969.944 kb on - strand at 969.970 kb on - strand at 969.970 kb on - strand at 970.005 kb on + strand at 970.023 kb on - strand at 970.105 kb on + strand, within CA265_RS04045 at 970.106 kb on - strand, within CA265_RS04045 at 970.183 kb on - strand, within CA265_RS04045 at 970.216 kb on + strand, within CA265_RS04045 at 970.230 kb on - strand, within CA265_RS04045 at 970.256 kb on + strand, within CA265_RS04045 at 970.274 kb on + strand, within CA265_RS04045 at 970.275 kb on - strand, within CA265_RS04045 at 970.304 kb on + strand, within CA265_RS04045 at 970.378 kb on + strand at 970.378 kb on + strand at 970.379 kb on - strand at 970.396 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.016 mg/ml remove 967,039 - CA265_RS04030 0.23 -0.2 967,088 + CA265_RS04030 0.27 +1.2 967,089 - CA265_RS04030 0.27 +0.0 967,123 + CA265_RS04030 0.30 +0.8 967,184 + CA265_RS04030 0.35 -0.7 967,282 + CA265_RS04030 0.43 -1.1 967,282 + CA265_RS04030 0.43 -1.6 967,282 + CA265_RS04030 0.43 -1.0 967,282 + CA265_RS04030 0.43 -0.2 967,294 + CA265_RS04030 0.44 -0.0 967,295 - CA265_RS04030 0.45 -2.1 967,352 - CA265_RS04030 0.49 -1.6 967,367 + CA265_RS04030 0.51 +0.0 967,368 - CA265_RS04030 0.51 -1.8 967,381 + CA265_RS04030 0.52 +0.7 967,381 - CA265_RS04030 0.52 +0.0 967,417 + CA265_RS04030 0.55 -0.4 967,417 + CA265_RS04030 0.55 -3.4 967,418 - CA265_RS04030 0.55 -0.8 967,418 - CA265_RS04030 0.55 +1.0 967,418 - CA265_RS04030 0.55 -0.2 967,421 + CA265_RS04030 0.55 -0.0 967,506 + CA265_RS04030 0.62 -0.8 967,506 + CA265_RS04030 0.62 +0.1 967,506 + CA265_RS04030 0.62 -0.6 967,538 - CA265_RS04030 0.65 -0.1 967,587 - CA265_RS04030 0.69 +0.1 967,597 + CA265_RS04030 0.70 +0.3 967,598 - CA265_RS04030 0.70 +0.6 967,609 - CA265_RS04030 0.71 +0.2 967,675 - CA265_RS04030 0.76 +1.3 967,853 + -0.5 967,853 + +0.5 967,885 - +0.0 967,917 + -0.6 967,959 - +0.1 967,970 + +0.8 968,002 - -0.2 968,080 + -0.4 968,170 + CA265_RS04035 0.12 -0.6 968,171 - CA265_RS04035 0.12 -0.3 968,291 - CA265_RS04035 0.20 +0.5 968,336 + CA265_RS04035 0.23 -1.2 968,336 + CA265_RS04035 0.23 -0.3 968,337 - CA265_RS04035 0.23 -1.2 968,337 - CA265_RS04035 0.23 +0.7 968,370 - CA265_RS04035 0.25 -0.9 968,403 + CA265_RS04035 0.27 -2.1 968,403 + CA265_RS04035 0.27 -1.6 968,404 - CA265_RS04035 0.27 -0.9 968,412 + CA265_RS04035 0.28 -0.1 968,434 + CA265_RS04035 0.29 +0.6 968,434 + CA265_RS04035 0.29 +0.0 968,435 - CA265_RS04035 0.29 -1.2 968,451 - CA265_RS04035 0.30 -0.2 968,457 + CA265_RS04035 0.31 -1.2 968,515 - CA265_RS04035 0.35 +0.1 968,559 - CA265_RS04035 0.38 +0.3 968,561 - CA265_RS04035 0.38 +1.1 968,561 - CA265_RS04035 0.38 +0.5 968,608 + CA265_RS04035 0.41 +0.0 968,677 - CA265_RS04035 0.45 +1.1 968,784 + CA265_RS04035 0.53 +0.3 968,872 + CA265_RS04035 0.58 -0.4 968,872 + CA265_RS04035 0.58 -1.8 968,885 + CA265_RS04035 0.59 -1.6 968,886 - CA265_RS04035 0.59 +0.3 968,886 - CA265_RS04035 0.59 +0.1 968,970 + CA265_RS04035 0.65 -1.4 968,987 - CA265_RS04035 0.66 +0.5 969,097 + CA265_RS04035 0.73 -0.7 969,098 - CA265_RS04035 0.73 +0.4 969,299 + CA265_RS04035 0.87 -0.3 969,369 - -0.2 969,374 - +0.1 969,479 - +0.5 969,543 + -0.9 969,584 - CA265_RS04040 0.15 -0.8 969,595 + CA265_RS04040 0.19 -1.8 969,596 - CA265_RS04040 0.19 -0.4 969,596 - CA265_RS04040 0.19 -1.2 969,600 - CA265_RS04040 0.20 +0.0 969,892 - +0.3 969,927 + -1.0 969,928 - +1.1 969,944 - -0.5 969,970 - -1.2 969,970 - -0.2 970,005 + +1.3 970,023 - -0.3 970,105 + CA265_RS04045 0.30 -2.5 970,106 - CA265_RS04045 0.30 -0.2 970,183 - CA265_RS04045 0.50 -0.2 970,216 + CA265_RS04045 0.58 -0.8 970,230 - CA265_RS04045 0.62 +1.1 970,256 + CA265_RS04045 0.68 -1.3 970,274 + CA265_RS04045 0.73 +0.8 970,275 - CA265_RS04045 0.73 -0.6 970,304 + CA265_RS04045 0.80 -2.5 970,378 + -1.1 970,378 + -1.4 970,379 - +0.4 970,396 + -1.2
Or see this region's nucleotide sequence