Experiment: R2A with Polymyxin B sulfate 0.016 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS02550 and CA265_RS02555 are separated by 59 nucleotides CA265_RS02555 and CA265_RS02560 are separated by 320 nucleotides CA265_RS02560 and CA265_RS02565 are separated by 67 nucleotides CA265_RS02565 and CA265_RS02570 are separated by 266 nucleotides
CA265_RS02550: CA265_RS02550 - tRNA-Phe, at 641,889 to 641,961
_RS02550
CA265_RS02555: CA265_RS02555 - ATPase, at 642,021 to 642,470
_RS02555
CA265_RS02560: CA265_RS02560 - hypothetical protein, at 642,791 to 644,398
_RS02560
CA265_RS02565: CA265_RS02565 - hypothetical protein, at 644,466 to 645,068
_RS02565
CA265_RS02570: CA265_RS02570 - GNAT family N-acetyltransferase, at 645,335 to 645,817
_RS02570
Position (kb) 642
643
644
645 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 641.867 kb on + strand at 641.897 kb on - strand, within CA265_RS02550 at 641.964 kb on + strand at 641.981 kb on - strand at 642.018 kb on + strand at 642.030 kb on + strand at 642.037 kb on + strand at 642.037 kb on + strand at 642.071 kb on + strand, within CA265_RS02555 at 642.208 kb on - strand, within CA265_RS02555 at 642.228 kb on + strand, within CA265_RS02555 at 642.386 kb on + strand, within CA265_RS02555 at 642.387 kb on - strand, within CA265_RS02555 at 642.391 kb on + strand, within CA265_RS02555 at 642.453 kb on - strand at 642.498 kb on + strand at 642.499 kb on - strand at 642.504 kb on + strand at 642.554 kb on + strand at 642.555 kb on - strand at 642.598 kb on - strand at 642.602 kb on - strand at 642.602 kb on - strand at 642.672 kb on + strand at 642.744 kb on - strand at 642.762 kb on - strand at 642.775 kb on + strand at 642.788 kb on + strand at 642.800 kb on - strand at 642.834 kb on + strand at 642.974 kb on + strand, within CA265_RS02560 at 643.047 kb on + strand, within CA265_RS02560 at 643.048 kb on - strand, within CA265_RS02560 at 643.050 kb on - strand, within CA265_RS02560 at 643.064 kb on + strand, within CA265_RS02560 at 643.094 kb on + strand, within CA265_RS02560 at 643.238 kb on - strand, within CA265_RS02560 at 643.265 kb on + strand, within CA265_RS02560 at 643.329 kb on + strand, within CA265_RS02560 at 643.330 kb on - strand, within CA265_RS02560 at 643.453 kb on + strand, within CA265_RS02560 at 643.453 kb on + strand at 643.456 kb on - strand, within CA265_RS02560 at 643.456 kb on - strand, within CA265_RS02560 at 643.486 kb on - strand, within CA265_RS02560 at 643.537 kb on - strand, within CA265_RS02560 at 643.543 kb on + strand, within CA265_RS02560 at 643.543 kb on + strand, within CA265_RS02560 at 643.543 kb on + strand, within CA265_RS02560 at 643.576 kb on + strand, within CA265_RS02560 at 643.577 kb on - strand, within CA265_RS02560 at 643.629 kb on + strand, within CA265_RS02560 at 643.634 kb on + strand, within CA265_RS02560 at 643.635 kb on - strand, within CA265_RS02560 at 643.650 kb on - strand, within CA265_RS02560 at 643.676 kb on - strand, within CA265_RS02560 at 643.712 kb on - strand, within CA265_RS02560 at 643.717 kb on + strand, within CA265_RS02560 at 643.725 kb on - strand, within CA265_RS02560 at 643.736 kb on + strand, within CA265_RS02560 at 643.737 kb on - strand, within CA265_RS02560 at 643.797 kb on + strand, within CA265_RS02560 at 643.816 kb on - strand, within CA265_RS02560 at 643.924 kb on - strand, within CA265_RS02560 at 643.940 kb on + strand, within CA265_RS02560 at 643.946 kb on - strand, within CA265_RS02560 at 644.014 kb on - strand, within CA265_RS02560 at 644.020 kb on - strand, within CA265_RS02560 at 644.036 kb on + strand, within CA265_RS02560 at 644.071 kb on - strand, within CA265_RS02560 at 644.074 kb on + strand, within CA265_RS02560 at 644.075 kb on - strand, within CA265_RS02560 at 644.075 kb on - strand, within CA265_RS02560 at 644.087 kb on + strand, within CA265_RS02560 at 644.087 kb on + strand, within CA265_RS02560 at 644.087 kb on + strand, within CA265_RS02560 at 644.114 kb on - strand, within CA265_RS02560 at 644.217 kb on - strand, within CA265_RS02560 at 644.228 kb on + strand, within CA265_RS02560 at 644.255 kb on - strand at 644.255 kb on - strand at 644.277 kb on + strand at 644.278 kb on - strand at 644.363 kb on - strand at 644.374 kb on + strand at 644.375 kb on - strand at 644.396 kb on + strand at 644.425 kb on + strand at 644.451 kb on + strand at 644.480 kb on + strand at 644.490 kb on - strand at 644.622 kb on - strand, within CA265_RS02565 at 644.622 kb on - strand, within CA265_RS02565 at 644.646 kb on - strand, within CA265_RS02565 at 644.652 kb on + strand, within CA265_RS02565 at 644.653 kb on - strand, within CA265_RS02565 at 644.757 kb on - strand, within CA265_RS02565 at 644.764 kb on - strand, within CA265_RS02565 at 644.952 kb on + strand, within CA265_RS02565 at 644.953 kb on - strand, within CA265_RS02565 at 645.012 kb on + strand at 645.048 kb on + strand at 645.049 kb on - strand at 645.049 kb on - strand at 645.051 kb on + strand at 645.052 kb on - strand at 645.090 kb on + strand at 645.091 kb on - strand at 645.092 kb on + strand at 645.092 kb on + strand at 645.092 kb on + strand at 645.093 kb on - strand at 645.118 kb on + strand at 645.118 kb on + strand at 645.260 kb on + strand at 645.260 kb on + strand at 645.277 kb on - strand at 645.316 kb on - strand at 645.370 kb on + strand at 645.374 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.016 mg/ml remove 641,867 + +0.2 641,897 - CA265_RS02550 0.11 +0.3 641,964 + -2.5 641,981 - -0.3 642,018 + +0.4 642,030 + +0.3 642,037 + +0.3 642,037 + +1.6 642,071 + CA265_RS02555 0.11 +0.1 642,208 - CA265_RS02555 0.42 -0.1 642,228 + CA265_RS02555 0.46 +0.8 642,386 + CA265_RS02555 0.81 +1.3 642,387 - CA265_RS02555 0.81 -1.3 642,391 + CA265_RS02555 0.82 -0.2 642,453 - -0.5 642,498 + +0.1 642,499 - -1.5 642,504 + +2.2 642,554 + +0.3 642,555 - -0.2 642,598 - -1.0 642,602 - +0.6 642,602 - -1.0 642,672 + +1.5 642,744 - -0.6 642,762 - -0.7 642,775 + +0.3 642,788 + -0.3 642,800 - -1.1 642,834 + -0.1 642,974 + CA265_RS02560 0.11 -2.3 643,047 + CA265_RS02560 0.16 -2.5 643,048 - CA265_RS02560 0.16 -2.2 643,050 - CA265_RS02560 0.16 -2.7 643,064 + CA265_RS02560 0.17 -1.7 643,094 + CA265_RS02560 0.19 -1.9 643,238 - CA265_RS02560 0.28 -2.0 643,265 + CA265_RS02560 0.29 -1.1 643,329 + CA265_RS02560 0.33 -1.2 643,330 - CA265_RS02560 0.34 -0.9 643,453 + CA265_RS02560 0.41 -1.3 643,453 + +1.5 643,456 - CA265_RS02560 0.41 -0.5 643,456 - CA265_RS02560 0.41 -1.0 643,486 - CA265_RS02560 0.43 -0.4 643,537 - CA265_RS02560 0.46 -0.4 643,543 + CA265_RS02560 0.47 -1.1 643,543 + CA265_RS02560 0.47 -0.8 643,543 + CA265_RS02560 0.47 -2.1 643,576 + CA265_RS02560 0.49 -0.2 643,577 - CA265_RS02560 0.49 -1.5 643,629 + CA265_RS02560 0.52 -1.0 643,634 + CA265_RS02560 0.52 -2.2 643,635 - CA265_RS02560 0.52 -1.1 643,650 - CA265_RS02560 0.53 -0.6 643,676 - CA265_RS02560 0.55 -1.3 643,712 - CA265_RS02560 0.57 +0.0 643,717 + CA265_RS02560 0.58 -1.1 643,725 - CA265_RS02560 0.58 -1.1 643,736 + CA265_RS02560 0.59 -1.8 643,737 - CA265_RS02560 0.59 -0.2 643,797 + CA265_RS02560 0.63 -1.2 643,816 - CA265_RS02560 0.64 -0.5 643,924 - CA265_RS02560 0.70 +0.1 643,940 + CA265_RS02560 0.71 -1.3 643,946 - CA265_RS02560 0.72 -1.1 644,014 - CA265_RS02560 0.76 -0.4 644,020 - CA265_RS02560 0.76 -0.7 644,036 + CA265_RS02560 0.77 -2.0 644,071 - CA265_RS02560 0.80 -1.0 644,074 + CA265_RS02560 0.80 -2.8 644,075 - CA265_RS02560 0.80 -0.5 644,075 - CA265_RS02560 0.80 -1.3 644,087 + CA265_RS02560 0.81 -2.6 644,087 + CA265_RS02560 0.81 -2.1 644,087 + CA265_RS02560 0.81 -0.3 644,114 - CA265_RS02560 0.82 -1.9 644,217 - CA265_RS02560 0.89 -1.5 644,228 + CA265_RS02560 0.89 -1.1 644,255 - -0.7 644,255 - -1.1 644,277 + -1.1 644,278 - +0.9 644,363 - -0.6 644,374 + -0.7 644,375 - +0.4 644,396 + -2.0 644,425 + -0.5 644,451 + -0.0 644,480 + +0.6 644,490 - -0.6 644,622 - CA265_RS02565 0.26 -0.3 644,622 - CA265_RS02565 0.26 -0.5 644,646 - CA265_RS02565 0.30 -1.1 644,652 + CA265_RS02565 0.31 -0.1 644,653 - CA265_RS02565 0.31 +0.6 644,757 - CA265_RS02565 0.48 -2.0 644,764 - CA265_RS02565 0.49 -0.4 644,952 + CA265_RS02565 0.81 -0.0 644,953 - CA265_RS02565 0.81 +0.0 645,012 + +0.5 645,048 + +0.2 645,049 - -2.1 645,049 - -1.6 645,051 + -0.5 645,052 - -0.7 645,090 + -0.1 645,091 - -0.1 645,092 + -0.5 645,092 + -1.5 645,092 + -0.8 645,093 - -0.1 645,118 + -0.0 645,118 + +0.2 645,260 + -0.2 645,260 + -0.1 645,277 - -0.7 645,316 - -0.1 645,370 + +0.1 645,374 - -0.1
Or see this region's nucleotide sequence