Experiment: R2A with Polymyxin B sulfate 0.016 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS01455 and CA265_RS01460 are separated by 54 nucleotides CA265_RS01460 and CA265_RS01465 are separated by 338 nucleotides CA265_RS01465 and CA265_RS01470 are separated by 262 nucleotides CA265_RS01470 and CA265_RS01475 are separated by 172 nucleotides
CA265_RS01455: CA265_RS01455 - MFS transporter, at 371,177 to 372,418
_RS01455
CA265_RS01460: CA265_RS01460 - hypothetical protein, at 372,473 to 372,841
_RS01460
CA265_RS01465: CA265_RS01465 - hypothetical protein, at 373,180 to 373,386
_RS01465
CA265_RS01470: CA265_RS01470 - tRNA-Ser, at 373,649 to 373,736
_RS01470
CA265_RS01475: CA265_RS01475 - DoxX family protein, at 373,909 to 374,355
_RS01475
Position (kb) 373
374 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 372.241 kb on + strand, within CA265_RS01455 at 372.246 kb on + strand, within CA265_RS01455 at 372.264 kb on + strand, within CA265_RS01455 at 372.278 kb on + strand, within CA265_RS01455 at 372.300 kb on + strand at 372.322 kb on + strand at 372.323 kb on - strand at 372.427 kb on - strand at 372.552 kb on + strand, within CA265_RS01460 at 372.568 kb on + strand, within CA265_RS01460 at 372.609 kb on + strand, within CA265_RS01460 at 372.651 kb on - strand, within CA265_RS01460 at 372.679 kb on + strand, within CA265_RS01460 at 372.734 kb on + strand, within CA265_RS01460 at 372.735 kb on - strand, within CA265_RS01460 at 372.735 kb on - strand, within CA265_RS01460 at 372.742 kb on - strand, within CA265_RS01460 at 372.742 kb on - strand, within CA265_RS01460 at 372.742 kb on - strand, within CA265_RS01460 at 372.742 kb on - strand, within CA265_RS01460 at 372.779 kb on - strand, within CA265_RS01460 at 372.881 kb on - strand at 372.916 kb on - strand at 372.941 kb on + strand at 372.942 kb on - strand at 372.943 kb on + strand at 372.965 kb on + strand at 372.966 kb on - strand at 372.969 kb on - strand at 372.985 kb on - strand at 373.026 kb on - strand at 373.027 kb on + strand at 373.127 kb on - strand at 373.130 kb on + strand at 373.206 kb on + strand, within CA265_RS01465 at 373.224 kb on + strand, within CA265_RS01465 at 373.225 kb on - strand, within CA265_RS01465 at 373.225 kb on - strand, within CA265_RS01465 at 373.244 kb on + strand, within CA265_RS01465 at 373.246 kb on + strand, within CA265_RS01465 at 373.248 kb on + strand, within CA265_RS01465 at 373.248 kb on + strand, within CA265_RS01465 at 373.322 kb on - strand, within CA265_RS01465 at 373.322 kb on - strand, within CA265_RS01465 at 373.336 kb on - strand, within CA265_RS01465 at 373.420 kb on + strand at 373.421 kb on - strand at 373.440 kb on + strand at 373.445 kb on + strand at 373.445 kb on + strand at 373.446 kb on - strand at 373.530 kb on + strand at 373.531 kb on - strand at 373.531 kb on - strand at 373.588 kb on - strand at 373.588 kb on - strand at 373.597 kb on + strand at 373.623 kb on - strand at 373.623 kb on - strand at 373.623 kb on - strand at 373.623 kb on - strand at 373.805 kb on - strand at 373.868 kb on + strand at 373.946 kb on - strand at 373.993 kb on + strand, within CA265_RS01475 at 373.997 kb on - strand, within CA265_RS01475 at 374.050 kb on - strand, within CA265_RS01475 at 374.089 kb on - strand, within CA265_RS01475 at 374.101 kb on + strand, within CA265_RS01475 at 374.124 kb on + strand, within CA265_RS01475 at 374.125 kb on - strand, within CA265_RS01475 at 374.131 kb on + strand, within CA265_RS01475 at 374.145 kb on + strand, within CA265_RS01475 at 374.145 kb on + strand, within CA265_RS01475 at 374.146 kb on - strand, within CA265_RS01475 at 374.149 kb on + strand, within CA265_RS01475 at 374.150 kb on - strand, within CA265_RS01475 at 374.150 kb on - strand, within CA265_RS01475 at 374.157 kb on + strand, within CA265_RS01475 at 374.179 kb on + strand, within CA265_RS01475 at 374.224 kb on - strand, within CA265_RS01475 at 374.272 kb on - strand, within CA265_RS01475 at 374.279 kb on - strand, within CA265_RS01475 at 374.281 kb on + strand, within CA265_RS01475 at 374.281 kb on + strand, within CA265_RS01475 at 374.282 kb on - strand, within CA265_RS01475 at 374.289 kb on + strand, within CA265_RS01475
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.016 mg/ml remove 372,241 + CA265_RS01455 0.86 +0.4 372,246 + CA265_RS01455 0.86 -0.4 372,264 + CA265_RS01455 0.88 -0.5 372,278 + CA265_RS01455 0.89 -0.1 372,300 + -0.6 372,322 + +0.7 372,323 - +0.4 372,427 - -2.3 372,552 + CA265_RS01460 0.21 -0.6 372,568 + CA265_RS01460 0.26 +0.8 372,609 + CA265_RS01460 0.37 -1.8 372,651 - CA265_RS01460 0.48 +0.1 372,679 + CA265_RS01460 0.56 -0.1 372,734 + CA265_RS01460 0.71 +0.2 372,735 - CA265_RS01460 0.71 +0.6 372,735 - CA265_RS01460 0.71 +1.0 372,742 - CA265_RS01460 0.73 -0.2 372,742 - CA265_RS01460 0.73 +1.3 372,742 - CA265_RS01460 0.73 +0.4 372,742 - CA265_RS01460 0.73 +0.5 372,779 - CA265_RS01460 0.83 +0.1 372,881 - -0.7 372,916 - -0.2 372,941 + -1.3 372,942 - +0.0 372,943 + -0.6 372,965 + +0.4 372,966 - +0.5 372,969 - -0.5 372,985 - +0.2 373,026 - +0.4 373,027 + -0.7 373,127 - +2.9 373,130 + +0.6 373,206 + CA265_RS01465 0.13 +0.6 373,224 + CA265_RS01465 0.21 -1.3 373,225 - CA265_RS01465 0.22 +0.0 373,225 - CA265_RS01465 0.22 +0.4 373,244 + CA265_RS01465 0.31 -0.4 373,246 + CA265_RS01465 0.32 -1.5 373,248 + CA265_RS01465 0.33 +0.1 373,248 + CA265_RS01465 0.33 -0.1 373,322 - CA265_RS01465 0.69 +0.2 373,322 - CA265_RS01465 0.69 -0.5 373,336 - CA265_RS01465 0.75 +0.3 373,420 + -0.7 373,421 - -0.6 373,440 + +0.7 373,445 + -0.2 373,445 + -0.8 373,446 - -0.5 373,530 + -1.4 373,531 - +1.1 373,531 - +0.0 373,588 - -0.2 373,588 - -0.2 373,597 + -1.3 373,623 - -0.4 373,623 - -0.3 373,623 - +1.0 373,623 - -0.1 373,805 - +0.4 373,868 + +0.5 373,946 - -0.0 373,993 + CA265_RS01475 0.19 -0.4 373,997 - CA265_RS01475 0.20 -0.7 374,050 - CA265_RS01475 0.32 -2.8 374,089 - CA265_RS01475 0.40 -1.1 374,101 + CA265_RS01475 0.43 +0.2 374,124 + CA265_RS01475 0.48 -1.1 374,125 - CA265_RS01475 0.48 -0.6 374,131 + CA265_RS01475 0.50 -0.4 374,145 + CA265_RS01475 0.53 +0.4 374,145 + CA265_RS01475 0.53 -0.8 374,146 - CA265_RS01475 0.53 -0.9 374,149 + CA265_RS01475 0.54 -0.8 374,150 - CA265_RS01475 0.54 -1.7 374,150 - CA265_RS01475 0.54 -1.6 374,157 + CA265_RS01475 0.55 +1.1 374,179 + CA265_RS01475 0.60 -1.1 374,224 - CA265_RS01475 0.70 -0.1 374,272 - CA265_RS01475 0.81 -2.0 374,279 - CA265_RS01475 0.83 -2.7 374,281 + CA265_RS01475 0.83 +0.8 374,281 + CA265_RS01475 0.83 +0.4 374,282 - CA265_RS01475 0.83 -1.6 374,289 + CA265_RS01475 0.85 -0.9
Or see this region's nucleotide sequence